KEGG   ENZYME: 1.14.13.18Help
Entry
EC 1.14.13.18               Enzyme                                 

Name
4-hydroxyphenylacetate 1-monooxygenase;
4-hydroxyphenylacetate 1-hydroxylase;
4-hydroxyphenylacetic 1-hydroxylase;
4-HPA 1-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
4-hydroxyphenylacetate,NAD(P)H:oxygen oxidoreductase (1-hydroxylating)
Reaction(IUBMB)
4-hydroxyphenylacetate + NAD(P)H + H+ + O2 = homogentisate + NAD(P)+ + H2O [RN:R02514 R02516]
Reaction(KEGG)
Substrate
4-hydroxyphenylacetate [CPD:C00642];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
homogentisate [CPD:C00544];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A flavoprotein (FAD). Also acts on 4-hydroxyhydratropate (forming 2-methylhomogentisate) and on 4-hydroxyphenoxyacetate (forming hydroquinone and glycolate).
History
EC 1.14.13.18 created 1976
Pathway
Tyrosine metabolism
Microbial metabolism in diverse environments
Reference
1  [PMID:234937]
  Authors
Hareland WA, Crawford RL, Chapman PJ, Dagley S.
  Title
Metabolic function and properties of 4-hydroxyphenylacetic acid 1-hydroxylase from Pseudomonas acidovorans.
  Journal
J. Bacteriol. 121 (1975) 272-85.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
55326-44-8
LinkDB All DBs

KEGG   REACTION: R02514Help
Entry
R02514                      Reaction                               

Name
4-hydroxyphenylacetate,NADH:oxygen oxidoreductase (1-hydroxylating)
Definition
4-Hydroxyphenylacetate + Oxygen + NADH + H+ <=> Homogentisate + NAD+ + H2O
Equation
Comment
multi-step reaction
NADPH (see R02516)
Reaction class
C00003_C00004
C00544_C00642
Enzyme
Pathway
Tyrosine metabolism
Microbial metabolism in diverse environments
Other DBs
RHEA: 
LinkDB All DBs

KEGG   REACTION: R02516Help
Entry
R02516                      Reaction                               

Name
4-hydroxyphenylacetate,NADPH:oxygen oxidoreductase (1-hydroxylating)
Definition
4-Hydroxyphenylacetate + Oxygen + NADPH + H+ <=> Homogentisate + NADP+ + H2O
Equation
Comment
multi-step reaction
NADH (see R02514)
Reaction class
C00005_C00006
C00544_C00642
Enzyme
Pathway
Tyrosine metabolism
Microbial metabolism in diverse environments
Other DBs
RHEA: 
LinkDB All DBs

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