KEGG   ORTHOLOGY: K00070Help
Entry
K00070                      KO                                     

Name
HSD3B
Definition
3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase [EC:1.1.1.145 5.3.3.1]
Pathway
Steroid hormone biosynthesis
Metabolic pathways
Ovarian steroidogenesis
Aldosterone synthesis and secretion
Module
M00107  
Steroid hormone biosynthesis, cholesterol => prognenolone => progesterone
M00110  
C19/C18-Steroid hormone biosynthesis, pregnenolone => androstenedione => estrone
Disease
H00216  
Congenital adrenal hyperplasia (CAH)
H00599  
46,XX disorders of sex development (Disorders related to androgen excess)
H00608  
46,XY disorders of sex development (Disorders in androgen synthesis or action)
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Lipid metabolism
   00140 Steroid hormone biosynthesis
    K00070  HSD3B; 3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
 Organismal Systems
  Endocrine system
   04913 Ovarian Steroidogenesis
    K00070  HSD3B; 3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
   04925 Aldosterone synthesis and secretion
    K00070  HSD3B; 3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
KEGG modules [BR:ko00002]
 Pathway module
  Carbohydrate and lipid metabolism
   Sterol biosynthesis
    M00107  Steroid hormone biosynthesis, cholesterol => prognenolone => progesterone
     K00070  HSD3B; 3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
    M00110  C19/C18-Steroid hormone biosynthesis, pregnenolone => androstenedione => estrone
     K00070  HSD3B; 3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.1  Acting on the CH-OH group of donors
   1.1.1  With NAD+ or NADP+ as acceptor
    1.1.1.145  3beta-hydroxy-Delta5-steroid dehydrogenase
     K00070  HSD3B; 3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
 5. Isomerases
  5.3  Intramolecular oxidoreductases
   5.3.3  Transposing C=C bonds
    5.3.3.1  steroid Delta-isomerase
     K00070  HSD3B; 3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
BRITE hierarchy
Other DBs
Genes
HSA: 
3283(HSD3B1) 3284(HSD3B2)
PTR: 
457165(HSD3B2) 469446(HSD3B1)
PPS: 
GGO: 
PON: 
100451655(HSD3B1) 100452394(HSD3B2)
NLE: 
MCC: 
712686(HSD3B2) 713091(HSD3B1)
MCF: 
CSAB: 
RRO: 
RBB: 
CJC: 
SBQ: 
MMU: 
100043456(Gm10681) 15492(Hsd3b1) 15493(Hsd3b2) 15495(Hsd3b4) 15496(Hsd3b5) 15497(Hsd3b6)
RNO: 
108348086 24470(Hsd3b5) 29632(Hsd3b2) 360348(Hsd3b1) 682974(Hsd3b3)
CGE: 
NGI: 
HGL: 
CCAN: 
OCU: 
TUP: 
CFA: 
483146(HSD3B2)
AML: 
100464280(HSD3B1)
UMR: 
ORO: 
FCA: 
100616070(HSD3B2)
PTG: 
AJU: 
BTA: 
281824(HSD3B1)
BOM: 
BIU: 
PHD: 
CHX: 
100860869(3BHSD)
OAS: 
100190978(HSD3B1)
SSC: 
445539(HSD3B1)
CFR: 
CDK: 
BACU: 
LVE: 
ECB: 
100034078(HSD3B2)
EPZ: 
EAI: 
106832920(HSD3B2)
MYB: 
106723550(HSD3B2)
HAI: 
109389868(HSD3B2)
RSS: 
109446820(HAO2)
PALE: 
LAV: 
100659049(HSD3B2)
TMU: 
MDO: 
SHR: 
100913530(HSD3B2)
OAA: 
GGA: 
396015(HSD3B1)
MGP: 
CJO: 
107319373(3beta-HSD)
APLA: 
ACYG: 
TGU: 
751770(HSD3B1)
GFR: 
FAB: 
PHI: 
PMAJ: 
CCW: 
FPG: 
FCH: 
CLV: 
EGZ: 
AAM: 
ASN: 
102367786(HSD3B1)
AMJ: 
109280168(HSD3B1)
PSS: 
CMY: 
CPIC: 
ACS: 
100561899(hsd3b1)
PVT: 
PBI: 
GJA: 
XLA: 
734818(hsd3b1.L)
XTR: 
NPR: 
DRE: 
373131(hsd3b2) 553532(hsd3b1)
SRX: 
SANH: 
SGH: 
CCAR: 
IPU: 
100304475(HSD3B)
AMEX: 
103027852(hsd3b1)
TRU: 
101069884(hsd3b1)
TNG: 
LCO: 
104921488(hsd3b1)
MZE: 
OLA: 
100144358(3bhsd)
XMA: 
PRET: 
NFU: 
107389612(hsd3b1)
CSEM: 
103391994(hsd3b1)
LCF: 
HCQ: 
BPEC: 
110156192(hsd3b1)
SASA: 
ELS: 
SFM: 
LCM: 
CMK: 
BFO: 
SKO: 
DME: 
DPO: 
DAN: 
DER: 
DSE: 
DSI: 
Dsimw501_GD14693(Dsim_GD14693)
DWI: 
DYA: 
DGR: 
DMO: 
DVI: 
MDE: 
AGA: 
AAG: 
CQU: 
NVI: 
ZNE: 
FCD: 
ISC: 
TUT: 
TSP: 
HRO: 
LGI: 
CRG: 
MYI: 
OBI: 
LAK: 
 » show all
TaxonomyKoalaUniProt
Reference
PMID:2243100
  Authors
Lachance Y, Luu-The V, Labrie C, Simard J, Dumont M, de Launoit Y, Guerin S, Leblanc G, Labrie F
  Title
Characterization of human 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase gene and its expression in mammalian cells.
  Journal
J Biol Chem 265:20469-75 (1990)
  Sequence
[hsa:3283] [rno:360348 682974]
LinkDB All DBs

KEGG   ENZYME: 1.1.1.145Help
Entry
EC 1.1.1.145                Enzyme                                 

Name
3beta-hydroxy-Delta5-steroid dehydrogenase;
progesterone reductase;
Delta5-3beta-hydroxysteroid dehydrogenase;
3beta-hydroxy-5-ene steroid dehydrogenase;
3beta-hydroxy steroid dehydrogenase/isomerase;
3beta-hydroxy-Delta5-C27-steroid dehydrogenase/isomerase;
3beta-hydroxy-Delta5-C27-steroid oxidoreductase;
3beta-hydroxy-5-ene-steroid oxidoreductase;
steroid-Delta5-3beta-ol dehydrogenase;
3beta-HSDH;
5-ene-3-beta-hydroxysteroid dehydrogenase;
3beta-hydroxy-5-ene-steroid dehydrogenase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
3beta-hydroxy-Delta5-steroid:NAD+ 3-oxidoreductase
Reaction(IUBMB)
a 3beta-hydroxy-Delta5-steroid + NAD+ = a 3-oxo-Delta5-steroid + NADH + H+ [RN:R03176]
Reaction(KEGG)
Substrate
3beta-hydroxy-Delta5-steroid [CPD:C03836];
NAD+ [CPD:C00003]
Product
3-oxo-Delta5-steroid [CPD:C01034];
NADH [CPD:C00004];
H+ [CPD:C00080]
Comment
This activity is found in several bifunctional enzymes that catalyse the oxidative conversion of Delta5-3-hydroxy steroids to a Delta4-3-oxo configuration. This conversion is carried out in two separate, sequential reactions; in the first reaction, which requires NAD+, the enzyme catalyses the dehydrogenation of the 3beta-hydroxy steroid to a 3-oxo intermediate. In the second reaction the reduced coenzyme, which remains attached to the enzyme, activates the isomerization of the Delta5 form to a Delta4 form (cf. EC 5.3.3.1, steroid Delta-isomerase). Substrates include dehydroepiandrosterone (which is converted into androst-5-ene-3,17-dione), pregnenolone (converted to progesterone) and cholest-5-en-3-one, an intermediate of cholesterol degradation.
History
EC 1.1.1.145 created 1972
Pathway
Steroid hormone biosynthesis
Steroid degradation
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K00070  
3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
K16045  
3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
Genes
HSA: 
3283(HSD3B1) 3284(HSD3B2)
PTR: 
457165(HSD3B2) 469446(HSD3B1)
PPS: 
GGO: 
PON: 
100451655(HSD3B1) 100452394(HSD3B2)
NLE: 
MCC: 
712686(HSD3B2) 713091(HSD3B1)
MCF: 
CSAB: 
RRO: 
RBB: 
CJC: 
SBQ: 
MMU: 
100043456(Gm10681) 15492(Hsd3b1) 15493(Hsd3b2) 15495(Hsd3b4) 15496(Hsd3b5) 15497(Hsd3b6)
RNO: 
108348086 24470(Hsd3b5) 29632(Hsd3b2) 360348(Hsd3b1) 682974(Hsd3b3)
CGE: 
NGI: 
HGL: 
CCAN: 
OCU: 
TUP: 
CFA: 
483146(HSD3B2)
AML: 
100464280(HSD3B1)
UMR: 
ORO: 
FCA: 
100616070(HSD3B2)
PTG: 
AJU: 
BTA: 
281824(HSD3B1)
BOM: 
BIU: 
PHD: 
CHX: 
100860869(3BHSD)
OAS: 
100190978(HSD3B1)
SSC: 
445539(HSD3B1)
CFR: 
CDK: 
BACU: 
LVE: 
ECB: 
100034078(HSD3B2)
EPZ: 
EAI: 
106832920(HSD3B2)
MYB: 
106723550(HSD3B2)
HAI: 
109389868(HSD3B2)
RSS: 
109446820(HAO2)
PALE: 
LAV: 
100659049(HSD3B2)
TMU: 
MDO: 
SHR: 
100913530(HSD3B2)
OAA: 
GGA: 
396015(HSD3B1)
MGP: 
CJO: 
107319373(3beta-HSD)
APLA: 
ACYG: 
TGU: 
751770(HSD3B1)
GFR: 
FAB: 
PHI: 
PMAJ: 
CCW: 
FPG: 
FCH: 
CLV: 
EGZ: 
AAM: 
ASN: 
102367786(HSD3B1)
AMJ: 
109280168(HSD3B1)
PSS: 
CMY: 
CPIC: 
ACS: 
100561899(hsd3b1)
PVT: 
PBI: 
GJA: 
XLA: 
734818(hsd3b1.L)
XTR: 
NPR: 
DRE: 
373131(hsd3b2) 553532(hsd3b1)
SRX: 
SANH: 
SGH: 
CCAR: 
IPU: 
100304475(HSD3B)
AMEX: 
103027852(hsd3b1)
TRU: 
101069884(hsd3b1)
TNG: 
LCO: 
104921488(hsd3b1)
MZE: 
OLA: 
100144358(3bhsd)
XMA: 
PRET: 
NFU: 
107389612(hsd3b1)
CSEM: 
103391994(hsd3b1)
LCF: 
HCQ: 
BPEC: 
110156192(hsd3b1)
SASA: 
ELS: 
SFM: 
LCM: 
CMK: 
BFO: 
SKO: 
DME: 
DPO: 
DAN: 
DER: 
DSE: 
DSI: 
Dsimw501_GD14693(Dsim_GD14693)
DWI: 
DYA: 
DGR: 
DMO: 
DVI: 
MDE: 
AGA: 
AAG: 
CQU: 
NVI: 
ZNE: 
FCD: 
ISC: 
TUT: 
TSP: 
HRO: 
LGI: 
CRG: 
MYI: 
OBI: 
LAK: 
MTU: 
MTV: 
MTC: 
MRA: 
MTF: 
MTB: 
MTK: 
MTZ: 
MTG: 
MTI: 
MTE: 
MTUR: 
MTL: 
MTO: 
MTD: 
MTN: 
MTJ: 
MTUB: 
MTUC: 
MTUE: 
MTX: 
MTUH: 
MTUL: 
MTUT: 
MTUU: 
MTQ: 
MBO: 
MBB: 
MBT: 
MBM: 
MBK: 
MBX: 
MBZ: 
MAF: 
MCE: 
MCQ: 
MCV: 
MCX: 
MCZ: 
MMIC: 
MLE: 
MLB: 
MPA: 
MAO: 
MAVI: 
MAVU: 
MAV: 
MAVD: 
MAVR: 
MAVA: 
MIT: 
MIA: 
MIE: 
MIR: 
MID: 
MYO: 
MCHI: 
MMAL: 
MSM: 
MSG: 
MSB: 
MSN: 
MSH: 
MSA: 
MUL: 
MVA: 
MGI: 
MSP: 
MMC: 
MKM: 
MJL: 
MJD: 
MMI: 
MMAE: 
MRH: 
MMM: 
MCB: 
MLI: 
MKN: 
MNE: 
MYV: 
MYE: 
MGO: 
MFT: 
MHAD: 
MPHL: 
MVQ: 
MYN: 
MLL: 
MDX: 
 » show all
Taxonomy
Reference
1  [PMID:4226148]
  Authors
Cheatum SG, Watten JC.
  Title
Purification and properties of 3-beta-hydroxysteroid dehydrogenase and delta-5-3-ketosteroid isomerase from bovine corpora lutea.
  Journal
Biochim. Biophys. Acta. 122 (1966) 1-13.
Reference
2  [PMID:14220672]
  Authors
KORITZ SB.
  Title
THE CONVERSION OF PREGNENOLONE TO PROGESTERONE BY SMALL PARTICLES FROM RAT ADRENAL.
  Journal
Biochemistry. 3 (1964) 1098-102.
Reference
3
  Authors
Neville, A.M., Orr, J.C. and Engel, L.L.
  Title
Delta5-3beta-Hydroxy steroid dehydrogenase activities of bovine adrenal cortex.
  Journal
Biochem. J. 107 (1968) 20.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9044-85-3
LinkDB All DBs

KEGG   REACTION: R02216Help
Entry
R02216                      Reaction                               

Name
Pregnenolone:NAD+ 3-oxidoreductase
Definition
Pregnenolone + NAD+ <=> Progesterone + NADH + H+
Equation
Comment
3beta-hydroxysteroid dehydrogenase/ Delta5-Delta4-isomerase
two-step reaction
Reaction class
C00003_C00004
C00410_C01953
Enzyme
1.1.1.145       5.3.3.1
Pathway
Steroid hormone biosynthesis
Metabolic pathways
Module
M00107  
Steroid hormone biosynthesis, cholesterol => prognenolone => progesterone
Orthology
K00070  
3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase [EC:1.1.1.145 5.3.3.1]
K01822  
steroid Delta-isomerase [EC:5.3.3.1]
LinkDB All DBs

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