KEGG ORTHOLOGY: K00365

ORTHOLOGY: K00365

Entry

K00365 KO

Name

uaZ

Definition

urate oxidase [EC:1.7.3.3]

Pathway

ko00230	Purine metabolism
ko00232	Caffeine metabolism

Module

M00546

Purine degradation, xanthine => urea

Brite

KEGG Orthology (KO) [BR:ko00001]
Metabolism
  Nucleotide metabolism
   00230 Purine metabolism
    K00365  uaZ; urate oxidase
  Biosynthesis of other secondary metabolites
   00232 Caffeine metabolism
    K00365  uaZ; urate oxidase
KEGG modules [BR:ko00002]
Pathway module
  Nucleotide and amino acid metabolism
   Purine metabolism
    M00546  Purine degradation, xanthine => urea
     K00365  uaZ; urate oxidase
Enzymes [BR:ko01000]
1. Oxidoreductases
  1.7  Acting on other nitrogenous compounds as donors
   1.7.3  With oxygen as acceptor
    1.7.3.3  factor-independent urate hydroxylase
     K00365  uaZ; urate oxidase

Other DBs

RN:

R02106 R07981

GO:

0004846

Genes

GGO:	101139849
PON:	100456896
NLE:	100597726
MCC:	714004(Uox)
MCF:	102117077(Uox)
CSAB:	103224536
RRO:	104677513
CJC:	100397154
SBQ:	101040936
MMU:	22262(Uox)
RNO:	114768(Uox)
CGE:	100768251
NGI:	103728098
HGL:	101719675
CCAN:	109688705
OCU:	100009266(UOX)
TUP:	102500418
CFA:	490189(UOX)
AML:	100470134
UMR:	103662970
FCA:	101096750
PTG:	102971100
AJU:	106973351
BTA:	514113(UOX)
BOM:	102267915
BIU:	109556136
PHD:	102330860
CHX:	102168228
OAS:	101109838
SSC:	397510(UOX)
CFR:	102505313
BACU:	103009966
LVE:	103070467
ECB:	100064919
MYB:	102244414
MYD:	102751767
HAI:	109385433 109385446
RSS:	109436574 109442137
PALE:	102878680
LAV:	100668450
MDO:	100012925
SHR:	100917431
OAA:	100078972
GGA:	101747367
MGP:	100551010
CJO:	107317543
APLA:	101797537
ACYG:	106033995
TGU:	100189932(UOX)
GFR:	102040716
FAB:	101806442
PHI:	102109233
CCW:	104695300
FPG:	101917753
FCH:	102059654
CLV:	102096622
AAM:	106499688
ASN:	102373915
AMJ:	102571917(UOX)
PSS:	102453497 102453977
CMY:	102932244(Uricase) 102932468(Uricase)
CPIC:	101936730 101944737
ACS:	100552598
PBI:	103058911
GJA:	107117302
XLA:	108714977 399031(MGC68684)
XTR:	496896(uox)
NPR:	108791380
DRE:	436604(uox)
SRX:	107742901
SANH:	107658581 107669002
SGH:	107560424
IPU:	100528126(uric)
TRU:	101078943
TNG:	GSTEN00022862G001
LCO:	104931943(Uricase)
NCC:	104941345
MZE:	101475077
OLA:	101159660
XMA:	102230976
CSEM:	103392251
LCF:	108881276
SASA:	100136464
ELS:	105008143
SFM:	108923494
LCM:	102360643
CMK:	103174706
BFO:	BRAFLDRAFT_113702
SPU:	588974
SKO:	100368654
DME:	Dmel_CG7171(Uro)
DPO:	Dpse_GA20153(Dpse_Uro)
DAN:	Dana_GF15322
DER:	Dere_GG10494
DPE:	Dper_GL19284
DSE:	Dsec_GM16558 Dsec_GM16560
DSI:	Dsimw501_GD23486(Dsim_GD23486)
DWI:	Dwil_GK24407 Dwil_GK24639
DYA:	Dyak_GE14614
DGR:	Dgri_GH19188 Dgri_GH19189
DMO:	Dmoj_GI24222
DVI:	Dvir_GJ10793(Dvir_Uro) Dvir_GJ26133
MDE:	101898809
AGA:	AgaP_AGAP008440
AAG:	AaeL_AAEL002194
CQU:	CpipJ_CPIJ003456
SOC:	105195412
AEC:	105151023
ACEP:	105619788
PBAR:	105424989
CFO:	105250801
NVI:	100123613
NVL:	108565014
BMOR:	692784
PXY:	105386323
DPX:	DAPPUDRAFT_233847
CRG:	105344267
NVE:	NEMVE_v1g175574
ADF:	107344624
ATH:	AT2G26230
ALY:	ARALYDRAFT_474714
CRB:	CARUB_v10020697mg
EUS:	EUTSA_v10021194mg
BRP:	103828734
BNA:	106357878 106452898
THJ:	104801755 104804947 104823771
CIT:	102631080
CIC:	CICLE_v10016037mg
TCC:	18586835
GRA:	105779785
GHI:	107927605 107943104
EGR:	104421156
GMX:	100037445(UOX) 547453(N-35)
PVU:	PHAVU_007G234300g
VRA:	106770791
VAR:	108326630
CCAJ:	109815339
MTR:	MTR_1g048370
CAM:	101500535(nodulin-35)
ADU:	107474240 107474983 107479189
AIP:	107621024 107622482 107625265 107625653
LJA:	Lj6g3v0143890.1(Lj6g3v0143890.1)
LANG:	109336232 109359424
FVE:	101304417
PPER:	18778286
PMUM:	103330981
MDM:	103418481 103438828
PXB:	103933611
ZJU:	107413131
CSV:	101206975
CMO:	103489372
RCU:	8272393
JCU:	105649139
POP:	POPTR_0010s24880g(POPTRDRAFT_822690)
VVI:	100232988
SLY:	100037732
SPEN:	107002894
SOT:	102581180
INI:	109147930
SIND:	105161011
BVG:	104907803
NNU:	104590108 104601391
OSA:	4324793
DOSA:	Os01t0865100-01(Os01g0865100)
OBR:	102704100
BDI:	100845980
ATS:	F775_07309
ZMA:	100274529
SITA:	101785812
PDA:	103721005
EGU:	105032853 105034366
MUS:	103979093
ATR:	18444423
SMO:	SELMODRAFT_156451 SELMODRAFT_172716
PPP:	PHYPADRAFT_143855
CRE:	CHLREDRAFT_183011(UOX)
VCN:	VOLCADRAFT_73602
BPG:	Bathy08g04420
MIS:	MICPUN_64532
CSL:	COCSUDRAFT_52384
CVR:	CHLNCDRAFT_20118
GSL:	Gasu_65630
CCP:	CHC_T00000418001
KLA:	KLLA0E10935g
LTH:	KLTH0H09262g
ZRO:	ZYRO0G09482g
TDL:	TDEL_0F02670(TDEL0F02670)
PPA:	PAS_chr2-2_0279
DHA:	DEHA2A07194g
PIC:	PICST_40781(URO1)
PGU:	PGUG_02092
SPAA:	SPAPADRAFT_136352
LEL:	LELG_01182
CAL:	CAALFM_C200180CA(CaO19.2114)
CTP:	CTRG_01093
COT:	CORT_0A12930
CDU:	CD36_15160
CTEN:	CANTEDRAFT_116017
YLI:	YALI0F09251g
CLU:	CLUG_05509
CAUR:	QG37_07102
NCR:	NCU07853
NTE:	NEUTE1DRAFT62263(NEUTE1DRAFT_62263)
SMP:	SMAC_02127
PAN:	PODANSg5539
TTT:	THITE_2171067
MTM:	MYCTH_2315024
CTHR:	CTHT_0071370
MGR:	MGG_05283
TMN:	UCRPA7_3858
FGR:	FGSG_04126
FPU:	FPSE_00146
FVR:	FVEG_10312
FOX:	FOXG_11511
NHE:	NECHADRAFT_81764
TRE:	TRIREDRAFT_76381
MAW:	MAC_01658
MAJ:	MAA_02492
CMT:	CCM_09432
VAL:	VDBG_06639
VDA:	VDAG_05692
CFJ:	CFIO01_08679
ELA:	UCREL1_184
PFY:	PFICI_05327
SSL:	SS1G_00106
BFU:	BC1G_06199
MBE:	MBM_07067
PSCO:	LY89DRAFT_688903
GLZ:	GLAREA_06901
ANI:	AN9470.2
AFM:	AFUA_2G10520
AOR:	AOR_1_994054(AO090011000588)
ANG:	ANI_1_1150034(An03g02330) ANI_1_826024(An02g06030)
AFV:	AFLA_044090
ACT:	ACLA_069530
NFI:	NFIA_085890
PCS:	Pc22g20960
PDP:	PDIP_63940
CIM:	CIMG_04344
CPW:	CPC735_073860
PBL:	PAAG_01437
PBN:	PADG_00331
URE:	UREG_01592
ABE:	ARB_05777
TVE:	TRV_04465
AJE:	HCAG_08071
PNO:	SNOG_08332
PTE:	PTT_12595
BZE:	COCCADRAFT_86661
BSC:	COCSADRAFT_124032
BOR:	COCMIDRAFT_82479
ZTR:	MYCGRDRAFT_70045
PFJ:	MYCFIDRAFT_184206
BCOM:	BAUCODRAFT_114390
NPA:	UCRNP2_5250
TML:	GSTUM_00004503001
SPO:	SPCC1223.09
CNE:	CNI02420
CNB:	CNBH2300
CGI:	CGB_H3440W
TMS:	TREMEDRAFT_70604
TVS:	TRAVEDRAFT_156281
DSQ:	DICSQDRAFT_145006 DICSQDRAFT_70560
PCO:	PHACADRAFT_251616
SHS:	STEHIDRAFT_117903
HIR:	HETIRDRAFT_439423
PSQ:	PUNSTDRAFT_91772
ADL:	AURDEDRAFT_59877
FME:	FOMMEDRAFT_102646
GTR:	GLOTRDRAFT_62332
LBC:	LACBIDRAFT_190858
MPR:	MPER_10554
MRR:	Moror_16610
CCI:	CC1G_04058
ABP:	AGABI1DRAFT42333(AGABI1DRAFT_42333)
ABV:	AGABI2DRAFT65743(AGABI2DRAFT_65743)
CPUT:	CONPUDRAFT_118951
SLA:	SERLADRAFT_356374
WSE:	WALSEDRAFT_59270
WIC:	J056_002062
UMA:	UMAG_00672
PFP:	PFL1_00057
PGR:	PGTG_04959
MLR:	MELLADRAFT_39009
DDI:	DDB_G0286427(uox)
DPP:	DICPUDRAFT_49836
DFA:	DFA_04065(uox)
ACAN:	ACA1_220120
PTI:	PHATRDRAFT_29736
PIF:	PITG_18410 PITG_18435 PITG_18437 PITG_22577
PSOJ:	PHYSODRAFT_469313 PHYSODRAFT_477479
SPAR:	SPRG_09685
GTT:	GUITHDRAFT_161824
NGR:	NAEGRDRAFT_80401
SCL:	sce4023
SCU:	SCE1572_24240
SME:	SM_b21284
SNO:	Snov_3388
MRD:	Mrad2831_0199
MET:	M446_5249
MNO:	Mnod_5813
MOR:	MOC_0208(pucL)
MAQU:	Maq22A_1p31805
PSIN:	CAK95_13210
MSM:	MSMEG_1296
MSG:	MSMEI_1258
MSB:	LJ00_06455
MSN:	LI99_06455
MSH:	LI98_06455
MAB:	MAB_2929c
MABB:	MASS_2864
MMV:	MYCMA_1623
MAY:	LA62_14885
MABO:	NF82_14655
MABL:	MMASJCM_2938
MAZ:	LA61_14785
MAK:	LH56_09415
MYS:	NF92_09560
MYC:	NF90_09560
MCHE:	BB28_14755
MIZ:	BAB75_15845
MGO:	AFA91_32355
ASD:	AS9A_4198
NFA:	NFA_52420
NFR:	ERS450000_04639(uox)
NBR:	O3I_016180 O3I_039235
NSL:	BOX37_30035
NSR:	NS506_06726
RHA:	RHA1_ro01568 RHA1_ro04509
RER:	RER_21140
REY:	O5Y_10090
REB:	XU06_10315
ROP:	ROP_12690 ROP_44290
ROA:	Pd630_LPD00958 Pd630_LPD05707
RPY:	Y013_22670
RHB:	NY08_1727 NY08_3502
RAV:	AAT18_25545
RFA:	A3L23_00088(uox)
SCO:	SCO6211(SC2G5.32)
SMA:	SAV_2018(pucL)
SGR:	SGR_1309
SGB:	WQO_28725
SCB:	SCAB_18521
SSX:	SACTE_5453
SVL:	Strvi_2410
SCT:	SCAT_4779
SCY:	SCATT_47730
SFA:	Sfla_1052
SBH:	SBI_02950
SHY:	SHJG_7271
SHO:	SHJGH_7031
SVE:	SVEN_2512 SVEN_6093
SDV:	BN159_2098(uox)
SALB:	XNR_0633
SALS:	SLNWT_0615
STRP:	F750_5793
SFI:	SFUL_6171
SCI:	B446_29175
SRC:	M271_13630
SALU:	DC74_6400
SALL:	SAZ_32150
SLV:	SLIV_07080
SGU:	SGLAU_26725
SVT:	SVTN_13450 SVTN_30295
STRE:	GZL_02614
SCW:	TU94_26730
SLD:	T261_1848
SXI:	SXIM_47140
STRM:	M444_18340 M444_27195
STRC:	AA958_28425
SAMB:	SAM23877_5951(uox)
SPRI:	SPRI_1552
SCZ:	ABE83_05550
SCX:	AS200_11350
SRW:	TUE45_06834(uox)
STRF:	ASR50_28400
SLE:	sle_14680(sle_14680)
SPAV:	Spa2297_26325
SLC:	SL103_25185
STRT:	A8713_25925
SCLF:	BB341_03990
SGS:	AVL59_12990
STSI:	A4E84_32100
SLS:	SLINC_6745
SNR:	SNOUR_11160
SPLU:	LK06_027275
STRD:	NI25_08290
SNW:	BBN63_05825
KSK:	KSE_63370
CMC:	CMN_00267
MIO:	AOA12_01320
MCW:	A8L33_04275
RTN:	A6122_1518
CUM:	NI26_12225
CUB:	BJK06_04275
FRP:	AX769_03080
AGY:	ATC03_05300
CART:	PA27867_1468
CRY:	B7495_07110(pucL)
CPHY:	B5808_11810
ART:	Arth_3427
ARR:	ARUE_c35390(uox)
ARL:	AFL94_16680
ARE:	AL755_03375
ARH:	AHiyo8_43670 AHiyo8_43690
ARY:	ATC04_01015
ARW:	MB46_14750
ARZ:	AUT26_16280
AAU:	AAur_3402
ACH:	Achl_3208
APN:	Asphe3_33450
PSUL:	AU252_13710
AAI:	AARI_33710
GAR:	AOZ07_17635
KRH:	KRH_19780
KFV:	AS188_04945
SATK:	SA2016_3345
BFA:	Bfae_08250
LMOI:	VV02_25695
CET:	B8281_09725
CCEU:	CBR64_01400
CFL:	Cfla_2783
SERJ:	SGUI_2186
BLY:	A2T55_04520
BLIN:	BLSMQ_0835
MPH:	MLP_22140
NCA:	Noca_1142
AER:	AERYTH_00570
NDA:	Ndas_0718
NAL:	B005_3625(pucL)
SRO:	Sros_2773
NOA:	BKM31_28615
FRA:	Francci3_1910
FRE:	Franean1_2243
FRI:	FraEuI1c_4801
FAL:	FRAAL4508
FSY:	FsymDg_1782
NML:	Namu_0238 Namu_0250
GOB:	Gobs_2838
MMAR:	MODMU_0113(uox)
KRA:	Krad_2071
SEN:	SACE_6736
AMD:	AMED_1926
AMN:	RAM_09770
AMM:	AMES_1910
AMZ:	B737_1912
AOI:	AORI_5946
AJA:	AJAP_09695(uox)
AMQ:	AMETH_1603
ALU:	BB31_14635
PDX:	Psed_1766
PSEA:	WY02_01130
PSEE:	FRP1_13340
PSEH:	XF36_13125
PSEQ:	AD006_21020
PECQ:	AD017_00600
PHH:	AFB00_24820
AMI:	Amir_5939
SESP:	BN6_71160(uox)
KAL:	KALB_4480
KPHY:	AOZ06_19875
LED:	BBK82_21895
ALL:	CRK55966
AMS:	AMIS_63790
ACTN:	L083_6156
AFS:	AFR_31535
CAI:	Caci_4737
SNA:	Snas_2538
TBI:	Tbis_2592
RXY:	Rxyl_2843
RRD:	RradSPS_1305
DRA:	DR_1160
DGE:	Dgeo_2601
DGO:	DGo_PA0216
DPD:	Deipe_0078
DSW:	QR90_03870
DPU:	SU48_09955
TRA:	Trad_1503
SACI:	Sinac_4646
PHM:	PSMK_30590
ACM:	AciX9_2872
GMA:	AciX8_0720
TSA:	AciPR4_3479
TRS:	Terro_0864
SUS:	Acid_0359
CPI:	Cpin_6768
NKO:	Niako_0442
HYP:	A0257_17680
HJE:	HacjB3_16061
HVO:	HVO_B0300

» show all

Reference

PMID:9360612

Authors

Colloc'h N, el Hajji M, Bachet B, L'Hermite G, Schiltz M, Prange T, Castro B, Mornon JP

Title

Crystal structure of the protein drug urate oxidase-inhibitor complex at 2.05 A resolution.

Journal

Nat Struct Biol 4:947-52 (1997)
DOI:10.1038/nsb1197-947

Sequence

[afv:AFLA_044090]

LinkDB

ORTHOLOGY: K16838

Entry

K16838 KO

Name

pucL

Definition

urate oxidase / 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase [EC:1.7.3.3 4.1.1.97]

Pathway

ko00230	Purine metabolism
ko00232	Caffeine metabolism

Module

M00546

Purine degradation, xanthine => urea

Brite

KEGG Orthology (KO) [BR:ko00001]
Metabolism
  Nucleotide metabolism
   00230 Purine metabolism
    K16838  pucL; urate oxidase / 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase
  Biosynthesis of other secondary metabolites
   00232 Caffeine metabolism
    K16838  pucL; urate oxidase / 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase
KEGG modules [BR:ko00002]
Pathway module
  Nucleotide and amino acid metabolism
   Purine metabolism
    M00546  Purine degradation, xanthine => urea
     K16838  pucL; urate oxidase / 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase
Enzymes [BR:ko01000]
1. Oxidoreductases
  1.7  Acting on other nitrogenous compounds as donors
   1.7.3  With oxygen as acceptor
    1.7.3.3  factor-independent urate hydroxylase
     K16838  pucL; urate oxidase / 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase
4. Lyases
  4.1  Carbon-carbon lyases
   4.1.1  Carboxy-lyases
    4.1.1.97  2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase
     K16838  pucL; urate oxidase / 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase

Other DBs

RN:

R02106 R06604 R07981

GO:

0004846 0051997

Genes

BSU:	BSU32450(pucL)
BSR:	I33_3345(pucL)
BSL:	A7A1_2459
BSH:	BSU6051_32450(pucL)
BSY:	I653_15640
BSUT:	BSUB_03463(pucL)
BSUL:	BSUA_03463(pucL)
BSUS:	Q433_17750
BSS:	BSUW23_15825(pucL)
BST:	GYO_3538(pucL)
BSO:	BSNT_09727(pucL)
BSN:	BSn5_07115
BSQ:	B657_32450(pucL)
BSX:	C663_3103(pucL)
BSP:	U712_16180
BHA:	BH0759
BCL:	ABC3735
BJS:	MY9_3258
BACI:	B1NLA3E_12615
BACY:	QF06_14540
BACL:	BS34A_35410(pucL)
BALM:	BsLM_3241
BEO:	BEH_09915
BSM:	BSM4216_2147
BFX:	BC359_11590
BGI:	BGM20_10325
GGH:	GHH_c21820(pucL)
GEA:	GARCT_02054(uao)
BSE:	Bsel_0582
PJD:	Pjdr2_3315
PMS:	KNP414_03236(uao)
PMQ:	PM3016_3460
PMW:	B2K_17880
POD:	PODO_06605
PAEF:	R50345_07070
PAEJ:	H70737_06880
PNP:	IJ22_16630
PXL:	BS614_20795
PYG:	AWM70_00910
PSWU:	SY83_16740
BTS:	Btus_2019
ABAC:	LuPra_02263(pucL_1)
NMO:	Nmlp_3624(pucL1)
HWC:	Hqrw_2223(pucL1)
NOU:	Natoc_3147

» show all

Reference

PMID:17567580

Authors

Kim K, Park J, Rhee S

Title

Structural and functional basis for (S)-allantoin formation in the ureide pathway.

Journal

J Biol Chem 282:23457-64 (2007)
DOI:10.1074/jbc.M703211200

Sequence

[ath:AT5G58220] [bsu:BSU32450]

LinkDB

ORTHOLOGY: K16839

Entry

K16839 KO

Name

hpxO

Definition

FAD-dependent urate hydroxylase [EC:1.14.13.113]

Pathway

ko00230

Purine metabolism

Module

M00546

Purine degradation, xanthine => urea

Brite

KEGG Orthology (KO) [BR:ko00001]
Metabolism
  Nucleotide metabolism
   00230 Purine metabolism
    K16839  hpxO; FAD-dependent urate hydroxylase
KEGG modules [BR:ko00002]
Pathway module
  Nucleotide and amino acid metabolism
   Purine metabolism
    M00546  Purine degradation, xanthine => urea
     K16839  hpxO; FAD-dependent urate hydroxylase
Enzymes [BR:ko01000]
1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.113  FAD-dependent urate hydroxylase
     K16839  hpxO; FAD-dependent urate hydroxylase

Other DBs

RN:

R09514

Genes

ENC:	ECL_02668
ENO:	ECENHK_07995
EEC:	EcWSU1_01566(nagX)
ENL:	A3UG_07945
ECLG:	EC036_15290
ECLN:	ECNIH4_14645
EAS:	Entas_1494
EAU:	DI57_10870
EKB:	BFV64_07975
ELG:	BH714_03925
ECLS:	LI67_009050
ENX:	NI40_007480
KPN:	KPN_01663
KPU:	KP1_2709
KPM:	KPHS_25970
KPP:	A79E_2563
KPK:	A593_23035
KPH:	KPNIH24_15295
KPZ:	KPNIH27_12610
KPV:	KPNIH29_13085
KPW:	KPNIH30_13415
KPY:	KPNIH31_12490
KPG:	KPNIH32_13265
KPC:	KPNIH10_12940
KPQ:	KPR0928_12960
KPT:	VK055_0825
KPE:	KPK_2712
KPO:	KPN2242_11215
KPR:	KPR_2563
KPJ:	N559_2645
KPI:	D364_08515
KPA:	KPNJ1_02826
KPS:	KPNJ2_02799
KPX:	PMK1_03994(hpxO)
KPB:	FH42_04315
KPNE:	KU54_013245
KPNU:	LI86_13180
KVA:	Kvar_2665
KVD:	KR75_20845
KVQ:	SP68_01810
KOX:	KOX_16735
KOE:	A225_2194
KOK:	KONIH1_10735
KOC:	AB185_25405
KOM:	HR38_15255
KQU:	AVR78_17435
EAE:	EAE_19215
EAR:	CCG30039
ROR:	RORB6_09725
RON:	TE10_10845
CED:	LH89_09680
PGE:	LG71_17645
KOR:	AWR26_16100
KRD:	A3780_08190
SPE:	Spro_2318
SRR:	SerAS9_2275
SRL:	SOD_c21490(hpxO)
SRY:	M621_11770
SPLY:	Q5A_011910(hpxO)
SRS:	SerAS12_2275
SRA:	SerAS13_2276
SMAF:	D781_1702
SLQ:	M495_11550
SRZ:	AXX16_3421
RAH:	Rahaq_4059
RAQ:	Rahaq2_4162
RAA:	Q7S_20625
DZE:	Dd1591_3565
DSO:	A4U42_11715
ETA:	ETA_17480
EPY:	EpC_18390
EPR:	EPYR_01981
EAM:	EAMY_1750
EAY:	EAM_1718
EBI:	EbC_22690
ERJ:	EJP617_28860
EGE:	EM595_1762(hpxO)
PAM:	PANA_1960(aba2)
PLF:	PANA5342_2219
PAJ:	PAJ_1292(aba2)
PAQ:	PAGR_g2145
PVA:	Pvag_1367(kmo)
PAO:	Pat9b_1955
KLN:	LH22_12690
PANT:	PSNIH1_13525
PANP:	PSNIH2_08370
PAGG:	AL522_14090
PAGC:	BEE12_16815
TCI:	A7K98_09905
POR:	APT59_07870
PRH:	LT40_12725
ACB:	A1S_3356
ABM:	ABSDF3498
ABY:	ABAYE0129
ABC:	ACICU_03553
ABN:	AB57_3806
ABB:	ABBFA_000127
ABX:	ABK1_3605
ABZ:	ABZJ_03745
ABR:	ABTJ_00125
ABD:	ABTW07_3761
ABH:	M3Q_95
ABAD:	ABD1_32520
ABJ:	BJAB07104_03649
ABAB:	BJAB0715_03737
ABAJ:	BJAB0868_03602
ABAZ:	P795_0640
ABK:	LX00_18140
ABAU:	IX87_13830
ABAA:	IX88_01560
ABW:	BL01_07135
ABAL:	ABLAC_01420
ACC:	BDGL_002824(aba2)
ANO:	RR32_00645
ACD:	AOLE_00610
ACI:	ACIAD3540
AEI:	AOY20_04610
ACW:	A0J50_00575 A0J50_08030 A0J50_18335
AHL:	AHTJS_06265
AJN:	BVL33_08235
ASOL:	BEN76_12465
ALA:	BFG52_06765
ASJ:	AsACE_CH00104(hpxO)
MOS:	AXE82_11135
CSA:	Csal_1784
HAA:	A5892_01090
KUS:	B9G99_09330
KMA:	B9H00_05520
MMW:	Mmwyl1_2710
MME:	Marme_3806
MPC:	Mar181_1373
KVU:	EIO_2118
KVL:	KVU_1677
KRO:	BVG79_01729
MPA:	MAP_4203
MAO:	MAP4_4328
MAVI:	RC58_21500
MAVU:	RE97_21550
MAV:	MAV_4429
MAVD:	NF84_20350
MAVR:	LA63_20550
MAVA:	LA64_20530
MIT:	OCO_43190
MIA:	OCU_42960
MIE:	LG41_20430
MIR:	OCQ_44310
MID:	MIP_06487
MYO:	OEM_43430
MSA:	Mycsm_00307 Mycsm_04326
MVA:	Mvan_5278
MCHE:	BB28_05800
MMI:	MMAR_4944
MMAE:	MMARE11_47540
MRH:	MycrhN_1446
MMM:	W7S_21580
MLI:	MULP_05189
MKN:	MKAN_19930
MKS:	LG40_19745
MNE:	D174_24580
MYV:	G155_23760
MFT:	XA26_48000
MHAD:	B586_12100
GBR:	Gbro_3294
GPO:	GPOL_c30910
GOR:	KTR9_3334
GTA:	BCM27_16930
TPR:	Tpau_2962
SRT:	Srot_0187
MTS:	MTES_1339
MIO:	AOA12_20870
MPAL:	BO218_10190
MIH:	BJP65_07865
CPHY:	B5808_14970
IDO:	I598_1238(hpxO_2)
ACTN:	L083_1264(kmo)
SYNP:	Syn7502_02605
DSL:	Dacsa_0407
CMP:	Cha6605_0874
LEN:	LEP3755_32360
LET:	O77CONTIG1_04142(hpxO)
LBO:	LBWT_43080
GLP:	Glo7428_4733
CTHE:	Chro_0933

» show all

Reference

PMID:19260710

Authors

O'Leary SE, Hicks KA, Ealick SE, Begley TP

Title

Biochemical characterization of the HpxO enzyme from Klebsiella pneumoniae, a novel FAD-dependent urate oxidase.

Journal

Biochemistry 48:3033-5 (2009)
DOI:10.1021/bi900160b

Sequence

[kpn:KPN_01663]

Reference

PMID:19060149

Authors

Pope SD, Chen LL, Stewart V

Title

Purine utilization by Klebsiella oxytoca M5al: genes for ring-oxidizing and -opening enzymes.

Journal

J Bacteriol 191:1006-17 (2009)
DOI:10.1128/JB.01281-08

Sequence

[kox:KOX_16735]

LinkDB

ENZYME: 1.7.3.3

Entry

EC 1.7.3.3 Enzyme

Name

factor-independent urate hydroxylase;
uric acid oxidase;
uricase;
uricase II;
urate oxidase

Class

Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With oxygen as acceptor

Sysname

urate:oxygen oxidoreductase

Reaction(IUBMB)

urate + O2 + H2O = 5-hydroxyisourate + H2O2 [RN:R02106]

Reaction(KEGG)

R02106;
(other) R07981

Substrate

urate [CPD:C00366];
O2 [CPD:C00007];
H2O [CPD:C00001]

Product

5-hydroxyisourate [CPD:C11821];
H2O2 [CPD:C00027]

Comment

This enzyme was previously thought to be a copper protein, but it is now known that the enzymes from soy bean (Glycine max), the mould Aspergillus flavus and Bacillus subtilis contains no copper nor any other transition-metal ion. The 5-hydroxyisourate formed decomposes spontaneously to form allantoin and CO2, although there is an enzyme-catalysed pathway in which EC 3.5.2.17, hydroxyisourate hydrolase, catalyses the first step. The enzyme is different from EC 1.14.13.113 (FAD-dependent urate hydroxylase).

History

EC 1.7.3.3 created 1961, modified 2002, modified 2005, modified 2010

Pathway

ec00230	Purine metabolism
ec00232	Caffeine metabolism
ec01100	Metabolic pathways
ec01120	Microbial metabolism in diverse environments

Orthology

K00365	urate oxidase
K16838	urate oxidase / 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase

Genes

GGO:	101139849
PON:	100456896
NLE:	100597726
MCC:	714004(Uox)
MCF:	102117077(Uox)
CSAB:	103224536
RRO:	104677513
CJC:	100397154
SBQ:	101040936
MMU:	22262(Uox)
RNO:	114768(Uox)
CGE:	100768251
NGI:	103728098
HGL:	101719675
CCAN:	109688705
OCU:	100009266(UOX)
TUP:	102500418
CFA:	490189(UOX)
AML:	100470134
UMR:	103662970
FCA:	101096750
PTG:	102971100
AJU:	106973351
BTA:	514113(UOX)
BOM:	102267915
BIU:	109556136
PHD:	102330860
CHX:	102168228
OAS:	101109838
SSC:	397510(UOX)
CFR:	102505313
BACU:	103009966
LVE:	103070467
ECB:	100064919
MYB:	102244414
MYD:	102751767
HAI:	109385433 109385446
RSS:	109436574 109442137
PALE:	102878680
LAV:	100668450
MDO:	100012925
SHR:	100917431
OAA:	100078972
GGA:	101747367
MGP:	100551010
CJO:	107317543
APLA:	101797537
ACYG:	106033995
TGU:	100189932(UOX)
GFR:	102040716
FAB:	101806442
PHI:	102109233
CCW:	104695300
FPG:	101917753
FCH:	102059654
CLV:	102096622
AAM:	106499688
ASN:	102373915
AMJ:	102571917(UOX)
PSS:	102453497 102453977
CMY:	102932244(Uricase) 102932468(Uricase)
CPIC:	101936730 101944737
ACS:	100552598
PBI:	103058911
GJA:	107117302
XLA:	108714977 399031(MGC68684)
XTR:	496896(uox)
NPR:	108791380
DRE:	436604(uox)
SRX:	107742901
SANH:	107658581 107669002
SGH:	107560424
IPU:	100528126(uric)
TRU:	101078943
TNG:	GSTEN00022862G001
LCO:	104931943(Uricase)
NCC:	104941345
MZE:	101475077
OLA:	101159660
XMA:	102230976
CSEM:	103392251
LCF:	108881276
SASA:	100136464
ELS:	105008143
SFM:	108923494
LCM:	102360643
CMK:	103174706
BFO:	BRAFLDRAFT_113702
SPU:	588974
SKO:	100368654
DME:	Dmel_CG7171(Uro)
DPO:	Dpse_GA20153(Dpse_Uro)
DAN:	Dana_GF15322
DER:	Dere_GG10494
DPE:	Dper_GL19284
DSE:	Dsec_GM16558 Dsec_GM16560
DSI:	Dsimw501_GD23486(Dsim_GD23486)
DWI:	Dwil_GK24407 Dwil_GK24639
DYA:	Dyak_GE14614
DGR:	Dgri_GH19188 Dgri_GH19189
DMO:	Dmoj_GI24222
DVI:	Dvir_GJ10793(Dvir_Uro) Dvir_GJ26133
MDE:	101898809
AGA:	AgaP_AGAP008440
AAG:	AaeL_AAEL002194
CQU:	CpipJ_CPIJ003456
SOC:	105195412
AEC:	105151023
ACEP:	105619788
PBAR:	105424989
CFO:	105250801
NVI:	100123613
NVL:	108565014
BMOR:	692784
PXY:	105386323
DPX:	DAPPUDRAFT_233847
CRG:	105344267
NVE:	NEMVE_v1g175574
ADF:	107344624
ATH:	AT2G26230
ALY:	ARALYDRAFT_474714
CRB:	CARUB_v10020697mg
EUS:	EUTSA_v10021194mg
BRP:	103828734
BNA:	106357878 106452898
THJ:	104801755 104804947 104823771
CIT:	102631080
CIC:	CICLE_v10016037mg
TCC:	18586835
GRA:	105779785
GHI:	107927605 107943104
EGR:	104421156
GMX:	100037445(UOX) 547453(N-35)
PVU:	PHAVU_007G234300g
VRA:	106770791
VAR:	108326630
CCAJ:	109815339
MTR:	MTR_1g048370
CAM:	101500535(nodulin-35)
ADU:	107474240 107474983 107479189
AIP:	107621024 107622482 107625265 107625653
LJA:	Lj6g3v0143890.1(Lj6g3v0143890.1)
LANG:	109336232 109359424
FVE:	101304417
PPER:	18778286
PMUM:	103330981
MDM:	103418481 103438828
PXB:	103933611
ZJU:	107413131
CSV:	101206975
CMO:	103489372
RCU:	8272393
JCU:	105649139
POP:	POPTR_0010s24880g(POPTRDRAFT_822690)
VVI:	100232988
SLY:	100037732
SPEN:	107002894
SOT:	102581180
INI:	109147930
SIND:	105161011
BVG:	104907803
NNU:	104590108 104601391
OSA:	4324793
DOSA:	Os01t0865100-01(Os01g0865100)
OBR:	102704100
BDI:	100845980
ATS:	F775_07309
ZMA:	100274529
SITA:	101785812
PDA:	103721005
EGU:	105032853 105034366
MUS:	103979093
ATR:	18444423
SMO:	SELMODRAFT_156451 SELMODRAFT_172716
PPP:	PHYPADRAFT_143855
CRE:	CHLREDRAFT_183011(UOX)
VCN:	VOLCADRAFT_73602
BPG:	Bathy08g04420
MIS:	MICPUN_64532
CSL:	COCSUDRAFT_52384
CVR:	CHLNCDRAFT_20118
GSL:	Gasu_65630
CCP:	CHC_T00000418001
KLA:	KLLA0E10935g
LTH:	KLTH0H09262g
ZRO:	ZYRO0G09482g
TDL:	TDEL_0F02670(TDEL0F02670)
PPA:	PAS_chr2-2_0279
DHA:	DEHA2A07194g
PIC:	PICST_40781(URO1)
PGU:	PGUG_02092
SPAA:	SPAPADRAFT_136352
LEL:	LELG_01182
CAL:	CAALFM_C200180CA(CaO19.2114)
CTP:	CTRG_01093
COT:	CORT_0A12930
CDU:	CD36_15160
CTEN:	CANTEDRAFT_116017
YLI:	YALI0F09251g
CLU:	CLUG_05509
CAUR:	QG37_07102
NCR:	NCU07853
NTE:	NEUTE1DRAFT62263(NEUTE1DRAFT_62263)
SMP:	SMAC_02127
PAN:	PODANSg5539
TTT:	THITE_2171067
MTM:	MYCTH_2315024
CTHR:	CTHT_0071370
MGR:	MGG_05283
TMN:	UCRPA7_3858
FGR:	FGSG_04126
FPU:	FPSE_00146
FVR:	FVEG_10312
FOX:	FOXG_11511
NHE:	NECHADRAFT_81764
TRE:	TRIREDRAFT_76381
MAW:	MAC_01658
MAJ:	MAA_02492
CMT:	CCM_09432
VAL:	VDBG_06639
VDA:	VDAG_05692
CFJ:	CFIO01_08679
ELA:	UCREL1_184
PFY:	PFICI_05327
SSL:	SS1G_00106
BFU:	BC1G_06199
MBE:	MBM_07067
PSCO:	LY89DRAFT_688903
GLZ:	GLAREA_06901
ANI:	AN9470.2
AFM:	AFUA_2G10520
AOR:	AOR_1_994054(AO090011000588)
ANG:	ANI_1_1150034(An03g02330) ANI_1_826024(An02g06030)
AFV:	AFLA_044090
ACT:	ACLA_069530
NFI:	NFIA_085890
PCS:	Pc22g20960
PDP:	PDIP_63940
CIM:	CIMG_04344
CPW:	CPC735_073860
PBL:	PAAG_01437
PBN:	PADG_00331
URE:	UREG_01592
ABE:	ARB_05777
TVE:	TRV_04465
AJE:	HCAG_08071
PNO:	SNOG_08332
PTE:	PTT_12595
BZE:	COCCADRAFT_86661
BSC:	COCSADRAFT_124032
BOR:	COCMIDRAFT_82479
ZTR:	MYCGRDRAFT_70045
PFJ:	MYCFIDRAFT_184206
BCOM:	BAUCODRAFT_114390
NPA:	UCRNP2_5250
TML:	GSTUM_00004503001
SPO:	SPCC1223.09
CNE:	CNI02420
CNB:	CNBH2300
CGI:	CGB_H3440W
TMS:	TREMEDRAFT_70604
TVS:	TRAVEDRAFT_156281
DSQ:	DICSQDRAFT_145006 DICSQDRAFT_70560
PCO:	PHACADRAFT_251616
SHS:	STEHIDRAFT_117903
HIR:	HETIRDRAFT_439423
PSQ:	PUNSTDRAFT_91772
ADL:	AURDEDRAFT_59877
FME:	FOMMEDRAFT_102646
GTR:	GLOTRDRAFT_62332
LBC:	LACBIDRAFT_190858
MPR:	MPER_10554
MRR:	Moror_16610
CCI:	CC1G_04058
ABP:	AGABI1DRAFT42333(AGABI1DRAFT_42333)
ABV:	AGABI2DRAFT65743(AGABI2DRAFT_65743)
CPUT:	CONPUDRAFT_118951
SLA:	SERLADRAFT_356374
WSE:	WALSEDRAFT_59270
WIC:	J056_002062
UMA:	UMAG_00672
PFP:	PFL1_00057
PGR:	PGTG_04959
MLR:	MELLADRAFT_39009
DDI:	DDB_G0286427(uox)
DPP:	DICPUDRAFT_49836
DFA:	DFA_04065(uox)
ACAN:	ACA1_220120
PTI:	PHATRDRAFT_29736
PIF:	PITG_18410 PITG_18435 PITG_18437 PITG_22577
PSOJ:	PHYSODRAFT_469313 PHYSODRAFT_477479
SPAR:	SPRG_09685
GTT:	GUITHDRAFT_161824
NGR:	NAEGRDRAFT_80401
SCL:	sce4023
SCU:	SCE1572_24240
SME:	SM_b21284
SNO:	Snov_3388
MRD:	Mrad2831_0199
MET:	M446_5249
MNO:	Mnod_5813
MOR:	MOC_0208(pucL)
MAQU:	Maq22A_1p31805
PSIN:	CAK95_13210
BSU:	BSU32450(pucL)
BSR:	I33_3345(pucL)
BSL:	A7A1_2459
BSH:	BSU6051_32450(pucL)
BSY:	I653_15640
BSUT:	BSUB_03463(pucL)
BSUL:	BSUA_03463(pucL)
BSUS:	Q433_17750
BSS:	BSUW23_15825(pucL)
BST:	GYO_3538(pucL)
BSO:	BSNT_09727(pucL)
BSN:	BSn5_07115
BSQ:	B657_32450(pucL)
BSX:	C663_3103(pucL)
BSP:	U712_16180
BHA:	BH0759
BCL:	ABC3735
BJS:	MY9_3258
BACI:	B1NLA3E_12615
BACY:	QF06_14540
BACL:	BS34A_35410(pucL)
BALM:	BsLM_3241
BEO:	BEH_09915
BSM:	BSM4216_2147
BFX:	BC359_11590
BGI:	BGM20_10325
GGH:	GHH_c21820(pucL)
GEA:	GARCT_02054(uao)
BSE:	Bsel_0582
PJD:	Pjdr2_3315
PMS:	KNP414_03236(uao)
PMQ:	PM3016_3460
PMW:	B2K_17880
POD:	PODO_06605
PAEF:	R50345_07070
PAEJ:	H70737_06880
PNP:	IJ22_16630
PXL:	BS614_20795
PYG:	AWM70_00910
PSWU:	SY83_16740
BTS:	Btus_2019
MSM:	MSMEG_1296
MSG:	MSMEI_1258
MSB:	LJ00_06455
MSN:	LI99_06455
MSH:	LI98_06455
MAB:	MAB_2929c
MABB:	MASS_2864
MMV:	MYCMA_1623
MAY:	LA62_14885
MABO:	NF82_14655
MABL:	MMASJCM_2938
MAZ:	LA61_14785
MAK:	LH56_09415
MYS:	NF92_09560
MYC:	NF90_09560
MCHE:	BB28_14755
MIZ:	BAB75_15845
MGO:	AFA91_32355
ASD:	AS9A_4198
NFA:	NFA_52420
NFR:	ERS450000_04639(uox)
NBR:	O3I_016180 O3I_039235
NSL:	BOX37_30035
NSR:	NS506_06726
RHA:	RHA1_ro01568 RHA1_ro04509
RER:	RER_21140
REY:	O5Y_10090
REB:	XU06_10315
ROP:	ROP_12690 ROP_44290
ROA:	Pd630_LPD00958 Pd630_LPD05707
RPY:	Y013_22670
RHB:	NY08_1727 NY08_3502
RAV:	AAT18_25545
RFA:	A3L23_00088(uox)
SCO:	SCO6211(SC2G5.32)
SMA:	SAV_2018(pucL)
SGR:	SGR_1309
SGB:	WQO_28725
SCB:	SCAB_18521
SSX:	SACTE_5453
SVL:	Strvi_2410
SCT:	SCAT_4779
SCY:	SCATT_47730
SFA:	Sfla_1052
SBH:	SBI_02950
SHY:	SHJG_7271
SHO:	SHJGH_7031
SVE:	SVEN_2512 SVEN_6093
SDV:	BN159_2098(uox)
SALB:	XNR_0633
SALS:	SLNWT_0615
STRP:	F750_5793
SFI:	SFUL_6171
SCI:	B446_29175
SRC:	M271_13630
SALU:	DC74_6400
SALL:	SAZ_32150
SLV:	SLIV_07080
SGU:	SGLAU_26725
SVT:	SVTN_13450 SVTN_30295
STRE:	GZL_02614
SCW:	TU94_26730
SLD:	T261_1848
SXI:	SXIM_47140
STRM:	M444_18340 M444_27195
STRC:	AA958_28425
SAMB:	SAM23877_5951(uox)
SPRI:	SPRI_1552
SCZ:	ABE83_05550
SCX:	AS200_11350
SRW:	TUE45_06834(uox)
STRF:	ASR50_28400
SLE:	sle_14680(sle_14680)
SPAV:	Spa2297_26325
SLC:	SL103_25185
STRT:	A8713_25925
SCLF:	BB341_03990
SGS:	AVL59_12990
STSI:	A4E84_32100
SLS:	SLINC_6745
SNR:	SNOUR_11160
SPLU:	LK06_027275
STRD:	NI25_08290
SNW:	BBN63_05825
KSK:	KSE_63370
CMC:	CMN_00267
MIO:	AOA12_01320
MCW:	A8L33_04275
RTN:	A6122_1518
CUM:	NI26_12225
CUB:	BJK06_04275
FRP:	AX769_03080
AGY:	ATC03_05300
CART:	PA27867_1468
CRY:	B7495_07110(pucL)
CPHY:	B5808_11810
ART:	Arth_3427
ARR:	ARUE_c35390(uox)
ARL:	AFL94_16680
ARE:	AL755_03375
ARH:	AHiyo8_43670 AHiyo8_43690
ARY:	ATC04_01015
ARW:	MB46_14750
ARZ:	AUT26_16280
AAU:	AAur_3402
ACH:	Achl_3208
APN:	Asphe3_33450
PSUL:	AU252_13710
AAI:	AARI_33710
GAR:	AOZ07_17635
KRH:	KRH_19780
KFV:	AS188_04945
SATK:	SA2016_3345
BFA:	Bfae_08250
LMOI:	VV02_25695
CET:	B8281_09725
CCEU:	CBR64_01400
CFL:	Cfla_2783
SERJ:	SGUI_2186
BLY:	A2T55_04520
BLIN:	BLSMQ_0835
MPH:	MLP_22140
NCA:	Noca_1142
AER:	AERYTH_00570
NDA:	Ndas_0718
NAL:	B005_3625(pucL)
SRO:	Sros_2773
NOA:	BKM31_28615
FRA:	Francci3_1910
FRE:	Franean1_2243
FRI:	FraEuI1c_4801
FAL:	FRAAL4508
FSY:	FsymDg_1782
NML:	Namu_0238 Namu_0250
GOB:	Gobs_2838
MMAR:	MODMU_0113(uox)
KRA:	Krad_2071
SEN:	SACE_6736
AMD:	AMED_1926
AMN:	RAM_09770
AMM:	AMES_1910
AMZ:	B737_1912
AOI:	AORI_5946
AJA:	AJAP_09695(uox)
AMQ:	AMETH_1603
ALU:	BB31_14635
PDX:	Psed_1766
PSEA:	WY02_01130
PSEE:	FRP1_13340
PSEH:	XF36_13125
PSEQ:	AD006_21020
PECQ:	AD017_00600
PHH:	AFB00_24820
AMI:	Amir_5939
SESP:	BN6_71160(uox)
KAL:	KALB_4480
KPHY:	AOZ06_19875
LED:	BBK82_21895
ALL:	CRK55966
AMS:	AMIS_63790
ACTN:	L083_6156
AFS:	AFR_31535
CAI:	Caci_4737
SNA:	Snas_2538
TBI:	Tbis_2592
RXY:	Rxyl_2843
RRD:	RradSPS_1305
DRA:	DR_1160
DGE:	Dgeo_2601
DGO:	DGo_PA0216
DPD:	Deipe_0078
DSW:	QR90_03870
DPU:	SU48_09955
TRA:	Trad_1503
SACI:	Sinac_4646
PHM:	PSMK_30590
ACM:	AciX9_2872
GMA:	AciX8_0720
TSA:	AciPR4_3479
TRS:	Terro_0864
SUS:	Acid_0359
ABAC:	LuPra_02263(pucL_1)
CPI:	Cpin_6768
NKO:	Niako_0442
HYP:	A0257_17680
HJE:	HacjB3_16061
NMO:	Nmlp_3624(pucL1)
HWC:	Hqrw_2223(pucL1)
HVO:	HVO_B0300
NOU:	Natoc_3147

» show all

Reference

1 [PMID:13363909]

Authors

HUDSON PB, LONDON M.

Title

Purification and properties of solubilized uricase.

Journal

Biochim. Biophys. Acta. 21 (1956) 290-8.

Reference

2 [PMID:13271340]

Authors

MAHLER HR, HUBSCHER G, BAUM R.

Title

Studies on uricase. I. Preparation, purification, and properties of a cuproprotein.

Journal

J. Biol. Chem. 216 (1955) 625-41.

Reference

3 [PMID:13252004]

Authors

ROBBINS KC, BARNETT EL, GRANT NH.

Title

Partial pruficiation of procine liver uricase.

Journal

J. Biol. Chem. 216 (1955) 27-35.

Reference

4 [PMID:9709003]

Authors

Kahn K, Tipton PA.

Title

Spectroscopic characterization of intermediates in the urate oxidase reaction.

Journal

Biochemistry. 37 (1998) 11651-9.

Reference

5 [PMID:9360612]

Authors

Colloc'h N, el Hajji M, Bachet B, L'Hermite G, Schiltz M, Prange T, Castro B, Mornon JP.

Title

Crystal structure of the protein drug urate oxidase-inhibitor complex at 2.05 A resolution.

Journal

Nat. Struct. Biol. 4 (1997) 947-52.

Sequence

[afv:AFLA_044090]

Reference

6 [PMID:12680763]

Authors

Imhoff RD, Power NP, Borrok MJ, Tipton PA.

Title

General base catalysis in the urate oxidase reaction: evidence for a novel Thr-Lys catalytic diad.

Journal

Biochemistry. 42 (2003) 4094-100.

Other DBs

ExplorEnz - The Enzyme Database:

1.7.3.3

IUBMB Enzyme Nomenclature:

1.7.3.3

ExPASy - ENZYME nomenclature database:

1.7.3.3

UM-BBD (Biocatalysis/Biodegradation Database):

1.7.3.3

BRENDA, the Enzyme Database:

1.7.3.3

CAS:

9002-12-4

LinkDB

ENZYME: 1.14.13.113

Entry

EC 1.14.13.113 Enzyme

Name

FAD-dependent urate hydroxylase;
HpxO enzyme;
FAD-dependent urate oxidase;
urate hydroxylase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

urate,NADH:oxygen oxidoreductase (5-hydroxyisourate forming)

Reaction(IUBMB)

urate + NADH + H+ + O2 = 5-hydroxyisourate + NAD+ + H2O [RN:R09514]

Reaction(KEGG)

R09514

Substrate

urate [CPD:C00366];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

5-hydroxyisourate [CPD:C11821];
NAD+ [CPD:C00003];
H2O [CPD:C00001]

Comment

A flavoprotein. The reaction is part of the purine catabolic pathway in the bacterium Klebsiella pneumoniae. The enzyme is different from EC 1.7.3.3, factor-independent urate hydroxylase, found in most plants, which produces hydrogen peroxide. The product of the enzyme is a substrate for EC 3.5.2.17, hydroxyisourate hydrolase.

History

EC 1.14.13.113 created 2010

Pathway

ec00230	Purine metabolism
ec01100	Metabolic pathways
ec01120	Microbial metabolism in diverse environments

Orthology

K16839

FAD-dependent urate hydroxylase

Genes

ENC:	ECL_02668
ENO:	ECENHK_07995
EEC:	EcWSU1_01566(nagX)
ENL:	A3UG_07945
ECLG:	EC036_15290
ECLN:	ECNIH4_14645
EAS:	Entas_1494
EAU:	DI57_10870
EKB:	BFV64_07975
ELG:	BH714_03925
ECLS:	LI67_009050
ENX:	NI40_007480
KPN:	KPN_01663
KPU:	KP1_2709
KPM:	KPHS_25970
KPP:	A79E_2563
KPK:	A593_23035
KPH:	KPNIH24_15295
KPZ:	KPNIH27_12610
KPV:	KPNIH29_13085
KPW:	KPNIH30_13415
KPY:	KPNIH31_12490
KPG:	KPNIH32_13265
KPC:	KPNIH10_12940
KPQ:	KPR0928_12960
KPT:	VK055_0825
KPE:	KPK_2712
KPO:	KPN2242_11215
KPR:	KPR_2563
KPJ:	N559_2645
KPI:	D364_08515
KPA:	KPNJ1_02826
KPS:	KPNJ2_02799
KPX:	PMK1_03994(hpxO)
KPB:	FH42_04315
KPNE:	KU54_013245
KPNU:	LI86_13180
KVA:	Kvar_2665
KVD:	KR75_20845
KVQ:	SP68_01810
KOX:	KOX_16735
KOE:	A225_2194
KOK:	KONIH1_10735
KOC:	AB185_25405
KOM:	HR38_15255
KQU:	AVR78_17435
EAE:	EAE_19215
EAR:	CCG30039
ROR:	RORB6_09725
RON:	TE10_10845
CED:	LH89_09680
PGE:	LG71_17645
KOR:	AWR26_16100
KRD:	A3780_08190
SPE:	Spro_2318
SRR:	SerAS9_2275
SRL:	SOD_c21490(hpxO)
SRY:	M621_11770
SPLY:	Q5A_011910(hpxO)
SRS:	SerAS12_2275
SRA:	SerAS13_2276
SMAF:	D781_1702
SLQ:	M495_11550
SRZ:	AXX16_3421
RAH:	Rahaq_4059
RAQ:	Rahaq2_4162
RAA:	Q7S_20625
DZE:	Dd1591_3565
DSO:	A4U42_11715
ETA:	ETA_17480
EPY:	EpC_18390
EPR:	EPYR_01981
EAM:	EAMY_1750
EAY:	EAM_1718
EBI:	EbC_22690
ERJ:	EJP617_28860
EGE:	EM595_1762(hpxO)
PAM:	PANA_1960(aba2)
PLF:	PANA5342_2219
PAJ:	PAJ_1292(aba2)
PAQ:	PAGR_g2145
PVA:	Pvag_1367(kmo)
PAO:	Pat9b_1955
KLN:	LH22_12690
PANT:	PSNIH1_13525
PANP:	PSNIH2_08370
PAGG:	AL522_14090
PAGC:	BEE12_16815
TCI:	A7K98_09905
POR:	APT59_07870
PRH:	LT40_12725
ACB:	A1S_3356
ABM:	ABSDF3498
ABY:	ABAYE0129
ABC:	ACICU_03553
ABN:	AB57_3806
ABB:	ABBFA_000127
ABX:	ABK1_3605
ABZ:	ABZJ_03745
ABR:	ABTJ_00125
ABD:	ABTW07_3761
ABH:	M3Q_95
ABAD:	ABD1_32520
ABJ:	BJAB07104_03649
ABAB:	BJAB0715_03737
ABAJ:	BJAB0868_03602
ABAZ:	P795_0640
ABK:	LX00_18140
ABAU:	IX87_13830
ABAA:	IX88_01560
ABW:	BL01_07135
ABAL:	ABLAC_01420
ACC:	BDGL_002824(aba2)
ANO:	RR32_00645
ACD:	AOLE_00610
ACI:	ACIAD3540
AEI:	AOY20_04610
ACW:	A0J50_00575 A0J50_08030 A0J50_18335
AHL:	AHTJS_06265
AJN:	BVL33_08235
ASOL:	BEN76_12465
ALA:	BFG52_06765
ASJ:	AsACE_CH00104(hpxO)
MOS:	AXE82_11135
CSA:	Csal_1784
HAA:	A5892_01090
KUS:	B9G99_09330
KMA:	B9H00_05520
MMW:	Mmwyl1_2710
MME:	Marme_3806
MPC:	Mar181_1373
KVU:	EIO_2118
KVL:	KVU_1677
KRO:	BVG79_01729
MPA:	MAP_4203
MAO:	MAP4_4328
MAVI:	RC58_21500
MAVU:	RE97_21550
MAV:	MAV_4429
MAVD:	NF84_20350
MAVR:	LA63_20550
MAVA:	LA64_20530
MIT:	OCO_43190
MIA:	OCU_42960
MIE:	LG41_20430
MIR:	OCQ_44310
MID:	MIP_06487
MYO:	OEM_43430
MSA:	Mycsm_00307 Mycsm_04326
MVA:	Mvan_5278
MCHE:	BB28_05800
MMI:	MMAR_4944
MMAE:	MMARE11_47540
MRH:	MycrhN_1446
MMM:	W7S_21580
MLI:	MULP_05189
MKN:	MKAN_19930
MKS:	LG40_19745
MNE:	D174_24580
MYV:	G155_23760
MFT:	XA26_48000
MHAD:	B586_12100
GBR:	Gbro_3294
GPO:	GPOL_c30910
GOR:	KTR9_3334
GTA:	BCM27_16930
TPR:	Tpau_2962
SRT:	Srot_0187
MTS:	MTES_1339
MIO:	AOA12_20870
MPAL:	BO218_10190
MIH:	BJP65_07865
CPHY:	B5808_14970
IDO:	I598_1238(hpxO_2)
ACTN:	L083_1264(kmo)
SYNP:	Syn7502_02605
DSL:	Dacsa_0407
CMP:	Cha6605_0874
LEN:	LEP3755_32360
LET:	O77CONTIG1_04142(hpxO)
LBO:	LBWT_43080
GLP:	Glo7428_4733
CTHE:	Chro_0933