| Entry |
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| Name |
2-oxo-3-hexenedioate decarboxylase;
4-oxalocrotonate carboxy-lyase (misleading);
4-oxalocrotonate decarboxylase (misleading);
cnbF (gene name);
praD (gene name);
amnE (gene name);
nbaG (gene name);
xylI (gene name)
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| Class |
Lyases;
Carbon-carbon lyases;
Carboxy-lyases
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| Sysname |
(3E)-2-oxohex-3-enedioate carboxy-lyase (2-oxopent-4-enoate-forming)
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| Reaction(IUBMB) |
(3E)-2-oxohex-3-enedioate = 2-oxopent-4-enoate + CO2 [RN: R02602]
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| Reaction(KEGG) |
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| Substrate |
(3E)-2-oxohex-3-enedioate
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| Product |
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| Comment |
Involved in the meta-cleavage pathway for the degradation of phenols, modified phenols and catechols. The enzyme has been reported to accept multiple tautomeric forms [1-4]. However, careful analysis of the stability of the different tautomers, as well as characterization of the enzyme that produces its substrate, EC 5.3.2.6, 2-hydroxymuconate tautomerase, showed that the actual substrate for the enzyme is (3E)-2-oxohex-3-enedioate [4].
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| History |
EC 4.1.1.77 created 1999, modified 2011, modified 2012
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| Pathway |
| Benzoate degradation | | Dioxin degradation | | Xylene degradation | | Metabolic pathways | | Microbial metabolism in diverse environments |
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| Orthology |
| 2-oxo-3-hexenedioate decarboxylase |
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| Genes |
PGE: | | SLQ: | | PSD: | | PPSE: | | PCQ: | | PPW: | | PPJ: | | PVR: | | PSC: | | PSTU: | | PBM: | | PKC: | | PALK: | | PSOS: | | PFK: | | AVN: | | AVL: | | AVD: | | ACX: | | SWD: | | MAD: | | MSR: | | MLQ: | | MSQ: | | LAL: | | CYQ: | | CZA: | | BLEP: | | HCS: | | APAC: | | GBI: | | TBN: | | AQL: | | RSO: | | RSM: | | RSE: | | RPI: | | RPF: | | RPJ: | | RIN: | | REU: | | REH: | | CNC: | | RME: | | CBW: | | CCUP: | | BVI: | | BUR: | | BCJ: | | BCEN: | | BCEO: | | BMUL: | | BCT: | | BCED: | | BCON: | | BDF: | | BTEI: | | BPSL: | | BUG: | | BUQ: | | BXE: | | BXB: | | BRH: | | BPX: | | BPY: | | BFN: | | PPK: | | PPNO: | | PPNM: | | PRB: | | PPUL: | | PSPU: | | PAPI: | | PVE: | | POX: | | PFG: | | PNR: | | BHZ: | | AKA: | | AMIM: | | CDN: | | POL: | | AJS: | | ACID: | | VEI: | | VPD: | | CTT: | | ADN: | | ADK: | | HYL: | | MPT: | | JAG: | | LCH: | | RBU: | | DAR: | | AZO: | | TMZ: | | THU: | | ARC: | | MXA: | | MFU: | | MSD: | | MYM: | | CCX: | | SUR: | | AGE: | | ARA: | | RLE: | | BRA: | | BBT: | | BIC: | | MRD: | | MET: | | MSL: | | MAAD: | | SUAM: | | NAR: | | NPP: | | NPN: | | SMAG: | | SMAZ: | | STER: | | SGI: | | SWI: | | SJP: | | SYB: | | SINB: | | ERY: | | CNA: | | MAGQ: | | BLI: | | BLD: | | BLH: | | BCA: | | BCX: | | BCF: | | BCER: | | BCEF: | | BTL: | | BMYC: | | BPF: | | BMQ: | | BMD: | | BMEG: | | BACI: | | GST: | | BACO: | | BSM: | | BSJ: | | BGY: | | BHK: | | GTN: | | GYC: | | GYA: | | GCT: | | GMC: | | GGH: | | GJF: | | GEJ: | | GTH: | | PTL: | | LGY: | | VIG: | | LAO: | | FPN: | | FAR: | | BBE: | | PNP: | | ANX: | | AAC: | | AAD: | | BTS: | | AUI: | | AVS: | | SAY: | | SAP: | | MIT: | | MIA: | | MVA: | | MJL: | | MYV: | | MGO: | | MFT: | | CHN: | | NFA: | | NFR: | | NNO: | | RHA: | | RER: | | REY: | | REB: | | ROP: | | REQ: | | RPY: | | RAV: | | GBR: | | GTA: | | SCB: | | SSX: | | SBH: | | SRC: | | STRT: | | MVD: | | AGY: | | ARZ: | | ICA: | | JTE: | | NCA: | | TCU: | | SRO: | | FRI: | | NML: | | AMD: | | AMN: | | AMM: | | AMZ: | | AOI: | | AJA: | | AMQ: | | ALU: | | PDX: | | ALL: | | STP: | | SAQ: | | MAU: | | MIL: | | VMA: | | IIS: | |
» show all
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| Reference |
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| Authors |
Shingler V, Powlowski J, Marklund U. |
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| Title |
Nucleotide sequence and functional analysis of the complete phenol/3,4-dimethylphenol catabolic pathway of Pseudomonas sp. strain CF600. |
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| Journal |
J. Bacteriol. 174 (1992) 711-24. |
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| Sequence |
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| Reference |
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| Authors |
Takenaka S, Murakami S, Shinke R, Aoki K |
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| Title |
Metabolism of 2-aminophenol by Pseudomonas sp. AP-3: modified meta-cleavage pathway. |
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| Journal |
Arch. Microbiol. 170 (1998) 132-7. |
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| Reference |
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| Authors |
Stanley TM, Johnson WH Jr, Burks EA, Whitman CP, Hwang CC, Cook PF |
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| Title |
Expression and stereochemical and isotope effect studies of active 4-oxalocrotonate decarboxylase. |
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| Journal |
Biochemistry. 39 (2000) 718-26. |
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| Reference |
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| Authors |
Wang SC, Johnson WH Jr, Czerwinski RM, Stamps SL, Whitman CP |
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| Title |
Kinetic and stereochemical analysis of YwhB, a 4-oxalocrotonate tautomerase homologue in Bacillus subtilis: mechanistic implications for the YwhB- and 4-oxalocrotonate tautomerase-catalyzed reactions. |
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| Journal |
Biochemistry. 46 (2007) 11919-29. |
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| Reference |
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| Authors |
Kasai D, Fujinami T, Abe T, Mase K, Katayama Y, Fukuda M, Masai E |
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| Title |
Uncovering the protocatechuate 2,3-cleavage pathway genes. |
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| Journal |
J. Bacteriol. 191 (2009) 6758-68. |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: UM-BBD (Biocatalysis/Biodegradation Database): BRENDA, the Enzyme Database: CAS: 37325-55-6 |
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| LinkDB |
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