KEGG ORTHOLOGY: K03268

ORTHOLOGY: K03268

Entry

K03268 Tight KO

Name

todC1, bedC1, tcbAa

Definition

benzene/toluene/chlorobenzene dioxygenase subunit alpha [EC:1.14.12.3 1.14.12.11 1.14.12.-]

Pathway

ko00361	Chlorocyclohexane and chlorobenzene degradation
ko00362	Benzoate degradation
ko00623	Toluene degradation
ko00625	Chloroalkane and chloroalkene degradation
ko01220	Degradation of aromatic compounds

Module

M00547

Benzene/toluene degradation, benzene => catechol / toluene => 3-methylcatechol

Brite

KEGG Orthology (KO) [BR:ko00001]
Metabolism
  Overview
   01220 Degradation of aromatic compounds
    K03268  todC1, bedC1, tcbAa; benzene/toluene/chlorobenzene dioxygenase subunit alpha
  Xenobiotics biodegradation and metabolism
   00362 Benzoate degradation
    K03268  todC1, bedC1, tcbAa; benzene/toluene/chlorobenzene dioxygenase subunit alpha
   00625 Chloroalkane and chloroalkene degradation
    K03268  todC1, bedC1, tcbAa; benzene/toluene/chlorobenzene dioxygenase subunit alpha
   00361 Chlorocyclohexane and chlorobenzene degradation
    K03268  todC1, bedC1, tcbAa; benzene/toluene/chlorobenzene dioxygenase subunit alpha
   00623 Toluene degradation
    K03268  todC1, bedC1, tcbAa; benzene/toluene/chlorobenzene dioxygenase subunit alpha
KEGG modules [BR:ko00002]
Pathway module
  Secondary metabolism
   Aromatics degradation
    M00547  Benzene/toluene degradation, benzene => catechol / toluene => 3-methylcatechol
     K03268  todC1, bedC1, tcbAa; benzene/toluene/chlorobenzene dioxygenase subunit alpha
Enzymes [BR:ko01000]
1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.12  With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
    1.14.12.3  benzene 1,2-dioxygenase
     K03268  todC1, bedC1, tcbAa; benzene/toluene/chlorobenzene dioxygenase subunit alpha
    1.14.12.11  toluene dioxygenase
     K03268  todC1, bedC1, tcbAa; benzene/toluene/chlorobenzene dioxygenase subunit alpha
    1.14.12.-
     K03268  todC1, bedC1, tcbAa; benzene/toluene/chlorobenzene dioxygenase subunit alpha

Other DBs

RN:

R03543 R03559 R05429 R06831 R06856 R09151 R09152

GO:

0018619 0018624 0018609

Genes

PPF:	Pput_2881
PPX:	T1E_4266(todC1)
PMON:	X969_10930
PMOT:	X970_10585
BPT:	Bpet3738
AG:	AAC43632(tcbAa)

Reference

PMID:8824602

Authors

Fong KP, Goh CB, Tan HM

Title

Characterization and expression of the plasmid-borne bedD gene from Pseudomonas putida ML2, which codes for a NAD+-dependent cis-benzene dihydrodiol dehydrogenase.

Journal

J Bacteriol 178:5592-601 (1996)
DOI:10.1128/JB.178.19.5592-5601.1996

Reference

PMID:2670929

Authors

Zylstra GJ, Gibson DT

Title

Toluene degradation by Pseudomonas putida F1. Nucleotide sequence of the todC1C2BADE genes and their expression in Escherichia coli.

Journal

J Biol Chem 264:14940-6 (1989)

Reference

PMID:8626733

Authors

Werlen C, Kohler HP, van der Meer JR

Title

The broad substrate chlorobenzene dioxygenase and cis-chlorobenzene dihydrodiol dehydrogenase of Pseudomonas sp. strain P51 are linked evolutionarily to the enzymes for benzene and toluene degradation.

Journal

J Biol Chem 271:4009-16 (1996)
DOI:10.1074/jbc.271.8.4009

Sequence

[ag:AAC43632]

LinkDB

ORTHOLOGY: K16268

Entry

K16268 Tight KO

Name

todC2, bedC2, tcbAb

Definition

benzene/toluene/chlorobenzene dioxygenase subunit beta [EC:1.14.12.3 1.14.12.11 1.14.12.-]

Pathway

ko00361	Chlorocyclohexane and chlorobenzene degradation
ko00362	Benzoate degradation
ko00623	Toluene degradation
ko00625	Chloroalkane and chloroalkene degradation
ko01220	Degradation of aromatic compounds

Module

M00547

Benzene/toluene degradation, benzene => catechol / toluene => 3-methylcatechol

Brite

KEGG Orthology (KO) [BR:ko00001]
Metabolism
  Overview
   01220 Degradation of aromatic compounds
    K16268  todC2, bedC2, tcbAb; benzene/toluene/chlorobenzene dioxygenase subunit beta
  Xenobiotics biodegradation and metabolism
   00362 Benzoate degradation
    K16268  todC2, bedC2, tcbAb; benzene/toluene/chlorobenzene dioxygenase subunit beta
   00625 Chloroalkane and chloroalkene degradation
    K16268  todC2, bedC2, tcbAb; benzene/toluene/chlorobenzene dioxygenase subunit beta
   00361 Chlorocyclohexane and chlorobenzene degradation
    K16268  todC2, bedC2, tcbAb; benzene/toluene/chlorobenzene dioxygenase subunit beta
   00623 Toluene degradation
    K16268  todC2, bedC2, tcbAb; benzene/toluene/chlorobenzene dioxygenase subunit beta
KEGG modules [BR:ko00002]
Pathway module
  Secondary metabolism
   Aromatics degradation
    M00547  Benzene/toluene degradation, benzene => catechol / toluene => 3-methylcatechol
     K16268  todC2, bedC2, tcbAb; benzene/toluene/chlorobenzene dioxygenase subunit beta
Enzymes [BR:ko01000]
1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.12  With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
    1.14.12.3  benzene 1,2-dioxygenase
     K16268  todC2, bedC2, tcbAb; benzene/toluene/chlorobenzene dioxygenase subunit beta
    1.14.12.11  toluene dioxygenase
     K16268  todC2, bedC2, tcbAb; benzene/toluene/chlorobenzene dioxygenase subunit beta
    1.14.12.-
     K16268  todC2, bedC2, tcbAb; benzene/toluene/chlorobenzene dioxygenase subunit beta

Other DBs

RN:

R03543 R03559 R05429 R06831 R06856 R09151 R09152

GO:

0018619 0018624 0018609

Genes

PPF:	Pput_2880
PPX:	T1E_4267(todC2)
PMON:	X969_10935
PMOT:	X970_10590
BPT:	Bpet3739
AG:	AAC43633(tcbAb)

Reference

PMID:8824602

Authors

Fong KP, Goh CB, Tan HM

Title

Characterization and expression of the plasmid-borne bedD gene from Pseudomonas putida ML2, which codes for a NAD+-dependent cis-benzene dihydrodiol dehydrogenase.

Journal

J Bacteriol 178:5592-601 (1996)
DOI:10.1128/JB.178.19.5592-5601.1996

Reference

PMID:2670929

Authors

Zylstra GJ, Gibson DT

Title

Toluene degradation by Pseudomonas putida F1. Nucleotide sequence of the todC1C2BADE genes and their expression in Escherichia coli.

Journal

J Biol Chem 264:14940-6 (1989)

Reference

PMID:8626733

Authors

Werlen C, Kohler HP, van der Meer JR

Title

Journal

J Biol Chem 271:4009-16 (1996)
DOI:10.1074/jbc.271.8.4009

Sequence

[ag:AAC43633]

LinkDB

ORTHOLOGY: K18089

Entry

K18089 Tight KO

Name

todB, tcbAc

Definition

benzene/toluene/chlorobenzene dioxygenase ferredoxin component

Pathway

ko00361	Chlorocyclohexane and chlorobenzene degradation
ko00362	Benzoate degradation
ko00623	Toluene degradation
ko00625	Chloroalkane and chloroalkene degradation
ko01220	Degradation of aromatic compounds

Module

M00547

Benzene/toluene degradation, benzene => catechol / toluene => 3-methylcatechol

Brite

KEGG Orthology (KO) [BR:ko00001]
Metabolism
  Overview
   01220 Degradation of aromatic compounds
    K18089  todB, tcbAc; benzene/toluene/chlorobenzene dioxygenase ferredoxin component
  Xenobiotics biodegradation and metabolism
   00362 Benzoate degradation
    K18089  todB, tcbAc; benzene/toluene/chlorobenzene dioxygenase ferredoxin component
   00625 Chloroalkane and chloroalkene degradation
    K18089  todB, tcbAc; benzene/toluene/chlorobenzene dioxygenase ferredoxin component
   00361 Chlorocyclohexane and chlorobenzene degradation
    K18089  todB, tcbAc; benzene/toluene/chlorobenzene dioxygenase ferredoxin component
   00623 Toluene degradation
    K18089  todB, tcbAc; benzene/toluene/chlorobenzene dioxygenase ferredoxin component
KEGG modules [BR:ko00002]
Pathway module
  Secondary metabolism
   Aromatics degradation
    M00547  Benzene/toluene degradation, benzene => catechol / toluene => 3-methylcatechol
     K18089  todB, tcbAc; benzene/toluene/chlorobenzene dioxygenase ferredoxin component

Other DBs

RN:

R03543 R03559 R05429 R06831 R06856 R09151 R09152

COG:

COG2146

Genes

PPF:	Pput_2879
PPX:	T1E_4268(tobB)
PMON:	X969_10940
PMOT:	X970_10595
BPT:	Bpet3740
AG:	AAC43634(tcbAc)

Reference

PMID:8626733

Authors

Werlen C, Kohler HP, van der Meer JR

Title

Journal

J Biol Chem 271:4009-16 (1996)
DOI:10.1074/jbc.271.8.4009

Sequence

[ag:AAC43634]

LinkDB

ORTHOLOGY: K18090

Entry

K18090 Tight KO

Name

todA, tcbAd

Definition

benzene/toluene/chlorobenzene dioxygenase ferredoxin reductase component [EC:1.18.1.3 1.18.1.-]

Pathway

ko00361	Chlorocyclohexane and chlorobenzene degradation
ko00362	Benzoate degradation
ko00623	Toluene degradation
ko00625	Chloroalkane and chloroalkene degradation
ko01220	Degradation of aromatic compounds

Module

M00547

Benzene/toluene degradation, benzene => catechol / toluene => 3-methylcatechol

Brite

KEGG Orthology (KO) [BR:ko00001]
Metabolism
  Overview
   01220 Degradation of aromatic compounds
    K18090  todA, tcbAd; benzene/toluene/chlorobenzene dioxygenase ferredoxin reductase component
  Xenobiotics biodegradation and metabolism
   00362 Benzoate degradation
    K18090  todA, tcbAd; benzene/toluene/chlorobenzene dioxygenase ferredoxin reductase component
   00625 Chloroalkane and chloroalkene degradation
    K18090  todA, tcbAd; benzene/toluene/chlorobenzene dioxygenase ferredoxin reductase component
   00361 Chlorocyclohexane and chlorobenzene degradation
    K18090  todA, tcbAd; benzene/toluene/chlorobenzene dioxygenase ferredoxin reductase component
   00623 Toluene degradation
    K18090  todA, tcbAd; benzene/toluene/chlorobenzene dioxygenase ferredoxin reductase component
KEGG modules [BR:ko00002]
Pathway module
  Secondary metabolism
   Aromatics degradation
    M00547  Benzene/toluene degradation, benzene => catechol / toluene => 3-methylcatechol
     K18090  todA, tcbAd; benzene/toluene/chlorobenzene dioxygenase ferredoxin reductase component
Enzymes [BR:ko01000]
1. Oxidoreductases
  1.18  Acting on iron-sulfur proteins as donors
   1.18.1  With NAD+ or NADP+ as acceptor
    1.18.1.3  ferredoxin---NAD+ reductase
     K18090  todA, tcbAd; benzene/toluene/chlorobenzene dioxygenase ferredoxin reductase component
    1.18.1.-
     K18090  todA, tcbAd; benzene/toluene/chlorobenzene dioxygenase ferredoxin reductase component

Other DBs

RN:

R03543 R03559 R05429 R06831 R06856 R09151 R09152

COG:

COG0446

GO:

0008860

Genes

PPF:	Pput_2878
PPX:	T1E_4269(tobA)
PMON:	X969_10945
PMOT:	X970_10600
BPT:	Bpet3741
AG:	AAC43635(tcbAd)

Reference

PMID:8626733

Authors

Werlen C, Kohler HP, van der Meer JR

Title

Journal

J Biol Chem 271:4009-16 (1996)
DOI:10.1074/jbc.271.8.4009

Sequence

[ag:AAC43635]

LinkDB

ENZYME: 1.14.12.11

Entry

EC 1.14.12.11 Enzyme

Name

toluene dioxygenase;
toluene 2,3-dioxygenase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor

Sysname

toluene,NADH:oxygen oxidoreductase (1,2-hydroxylating)

Reaction(IUBMB)

toluene + NADH + H+ + O2 = (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+ [RN:R03559]

Reaction(KEGG)

R03559;
(other) R09151 R09152

Substrate

toluene [CPD:C01455];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

(1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol [CPD:C04592];
NAD+ [CPD:C00003]

Comment

A system, containing a reductase which is an iron-sulfur flavoprotein (FAD), an iron-sulfur oxygenase, and a ferredoxin. Some other aromatic compounds, including ethylbenzene, 4-xylene and some halogenated toluenes, are converted into the corresponding cis-dihydrodiols.

History

EC 1.14.12.11 created 1992

Pathway

ec00623	Toluene degradation
ec00625	Chloroalkane and chloroalkene degradation
ec01120	Microbial metabolism in diverse environments

Orthology

K03268	benzene/toluene/chlorobenzene dioxygenase subunit alpha
K16268	benzene/toluene/chlorobenzene dioxygenase subunit beta

Genes

PPF:	Pput_2880 Pput_2881
PPX:	T1E_4266(todC1) T1E_4267(todC2)
PMON:	X969_10930 X969_10935
PMOT:	X970_10585 X970_10590
BPT:	Bpet3738 Bpet3739

Reference

1 [PMID:16347845]

Authors

Renganathan V.

Title

Possible Involvement of Toluene-2,3-Dioxygenase in Defluorination of 3-Fluoro-Substituted Benzenes by Toluene-Degrading Pseudomonas sp. Strain T-12.

Journal

Appl. Environ. Microbiol. 55 (1989) 330-334.

Reference

2 [PMID:526270]

Authors

Subramanian V, Liu TN, Yeh WK, Gibson DT.

Title

Toluene dioxygenase: purification of an iron-sulfur protein by affinity chromatography.

Journal

Biochem. Biophys. Res. Commun. 91 (1979) 1131-9.

Other DBs

ExplorEnz - The Enzyme Database:

1.14.12.11

IUBMB Enzyme Nomenclature:

1.14.12.11

ExPASy - ENZYME nomenclature database:

1.14.12.11

UM-BBD (Biocatalysis/Biodegradation Database):

1.14.12.11

BRENDA, the Enzyme Database:

1.14.12.11

CAS:

120038-36-0

LinkDB

REACTION: R09152

Entry

R09152 Reaction

Name

trichloroethene,NADH:oxygen oxidoreductase (formate-forming)

Definition

Trichloroethene + Oxygen + 2 H2O + NADH + H+ <=> 2 Formate + 3 Hydrochloric acid + NAD+

Equation

C06790 + C00007 + 2 C00001 + C00004 + C00080 <=> 2 C00058 + 3 C01327 + C00003

Comment

dioxygenase (distinct from R09141+R09145)
requiring ferredoxin and ferredoxin reductase (ec 1.18.1.3)

Reaction class

RC03012	C00058_C06790
RC03013	C00058_C06790

Enzyme

1.14.12.11

Pathway

rn00625	Chloroalkane and chloroalkene degradation
rn01120	Microbial metabolism in diverse environments

Orthology

K03268	benzene/toluene/chlorobenzene dioxygenase subunit alpha [EC:1.14.12.3 1.14.12.11 1.14.12.-]
K16268	benzene/toluene/chlorobenzene dioxygenase subunit beta [EC:1.14.12.3 1.14.12.11 1.14.12.-]
K18089	benzene/toluene/chlorobenzene dioxygenase ferredoxin component
K18090	benzene/toluene/chlorobenzene dioxygenase ferredoxin reductase component [EC:1.18.1.3 1.18.1.-]

LinkDB

DBGET integrated database retrieval system