| Entry |
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| Name |
magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase;
Mg-protoporphyrin IX monomethyl ester (oxidative) cyclase
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| Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
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| Sysname |
magnesium-protoporphyrin-IX 13-monomethyl ester,NADPH:oxygen oxidoreductase (hydroxylating)
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| Reaction(IUBMB) |
magnesium-protoporphyrin IX 13-monomethyl ester + 3 NADPH + 3 H+ + 3 O2 = 3,8-divinyl protochlorophyllide a + 3 NADP+ + 5 H2O (overall reaction) [RN: R10068];
(1a) magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 = 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O [RN: R06265];
(1b) 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 = 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + 2 H2O [RN: R06266];
(1c) 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 = 3,8-divinyl protochlorophyllide a + NADP+ + 2 H2O [RN: R06267]
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| Reaction(KEGG) |
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| Substrate |
magnesium-protoporphyrin IX 13-monomethyl ester [CPD: C04536];
NADPH [CPD: C00005];
H+ [CPD: C00080];
O2 [CPD: C00007];
131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester;
131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester
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| Product |
3,8-divinyl protochlorophyllide a [CPD: C11831];
NADP+ [CPD: C00006];
H2O [CPD: C00001];
131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester;
131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester
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| Comment |
Requires Fe(II) for activity. The enzyme participates in the biosynthesis of chlorophyllide a in aerobic organisms. The same transformation is achieved in anaerobic organisms by EC 1.21.98.3, anaerobic magnesium-protoporphyrin IX monomethyl ester cyclase. Some facultative phototrophic bacteria, such as Rubrivivax gelatinosus, possess both enzymes.
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| History |
EC 1.14.13.81 created 2003, modified 2017
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| Pathway |
| Porphyrin and chlorophyll metabolism | | Metabolic pathways | | Biosynthesis of secondary metabolites |
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| Orthology |
| magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase |
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| Genes |
ATH: | | ALY: | | CRB: | | EUS: | | BRP: | | BNA: | | THJ: | | CIT: | | CIC: | | TCC: | | GRA: | | GHI: | | EGR: | | GMX: | | PVU: | | VRA: | | VAR: | | CCAJ: | | MTR: | | CAM: | | ADU: | | AIP: | | LJA: | | LANG: | | FVE: | | PPER: | | PMUM: | | MDM: | | PXB: | | ZJU: | | CSV: | | CMO: | | RCU: | | JCU: | | POP: | | VVI: | | SLY: | | SPEN: | | SOT: | | INI: | | SIND: | | BVG: | | NNU: | | OSA: | | DOSA: | | OBR: | | BDI: | | ATS: | | SBI: | | ZMA: | | SITA: | | PDA: | | EGU: | | MUS: | | ATR: | | SMO: | | PPP: | | CRE: | | VCN: | | OLU: | | OTA: | | BPG: | | MIS: | | MPP: | | CSL: | | CVR: | | APRO: | | PDQ: | | RAC: | | LIM: | | LIH: | | HYR: | | RGE: | | RBN: | | RDP: | | METR: | | BEB: | | AGC: | | BRA: | | BBT: | | BRS: | | AOL: | | RPA: | | RPB: | | RPC: | | RPD: | | RPE: | | RPT: | | RPX: | | BOS: | | MEX: | | MEA: | | MDI: | | MCH: | | MRD: | | MET: | | MPO: | | MOR: | | META: | | MAQU: | | MSL: | | BSB: | | BRL: | | RSP: | | RSH: | | RSQ: | | RSK: | | JAN: | | RDE: | | RLI: | | DSH: | | RED: | | PTP: | | RSU: | | RHM: | | RHC: | | SUAM: | | TOM: | | RMM: | | LVS: | | HBC: | | SPHI: | | SSAN: | | CIJ: | | BLAS: | | ELQ: | | PNS: | | PORL: | | AMV: | | RCE: | | SYN: | | SYZ: | | SYY: | | SYT: | | SYS: | | SYQ: | | SYJ: | | SYW: | | SYC: | | SYF: | | SYD: | | SYE: | | SYG: | | SYR: | | SYX: | | SYP: | | CYA: | | CYB: | | SYNE: | | SYNP: | | SYNK: | | SYNR: | | SYND: | | SYU: | | SYH: | | SYNW: | | TEL: | | THN: | | CGC: | | CYI: | | DSL: | | CMP: | | LEP: | | LEN: | | LET: | | LBO: | | HHG: | | PSEU: | | PMA: | | PMM: | | PMT: | | PMN: | | PMI: | | PMB: | | PMC: | | PMF: | | PMG: | | PMH: | | PMJ: | | PME: | | PRC: | | PRM: | | AMR: | | MAR: | | MPK: | | CAN: | | CSN: | | HAO: | | CYU: | | GLP: | | GEN: | | GEE: | | CYT: | | CYP: | | CYC: | | CYN: | | CYH: | | CYJ: | | TER: | | MIC: | | ARP: | | GEI: | | OAC: | | ONI: | | MPRO: | | CEP: | | GVI: | | GLJ: | | ANA: | | NPU: | | NOS: | | NOP: | | NON: | | AVA: | | ANB: | | ACY: | | AWA: | | NAZ: | | CSG: | | CALO: | | CALT: | | CALH: | | RIV: | | FIS: | | NCN: | | CTHE: | | PLP: | | SCS: | | RRS: | | RCA: | | CAU: | | CAG: | | CHL: | | CTM: | | GPH: | |
» show all
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| Reference |
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| Authors |
Walker CJ, Mansfield KE, Rezzano IN, Hanamoto CM, Smith KM, Castelfranco PA |
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| Title |
The magnesium-protoporphyrin IX (oxidative) cyclase system. Studies on the mechanism and specificity of the reaction sequence. |
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| Journal |
Biochem. J. 255 (1988) 685-92. |
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| Reference |
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| Authors |
Bollivar DW, Beale SI. |
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| Title |
The Chlorophyll Biosynthetic Enzyme Mg-Protoporphyrin IX Monomethyl Ester (Oxidative) Cyclase (Characterization and Partial Purification from Chlamydomonas reinhardtii and Synechocystis sp. PCC 6803). |
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| Journal |
Plant. Physiol. 112 (1996) 105-114. |
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| Reference |
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| Authors |
Pinta V, Picaud M, Reiss-Husson F, Astier C |
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| Title |
Rubrivivax gelatinosus acsF (previously orf358) codes for a conserved, putative binuclear-iron-cluster-containing protein involved in aerobic oxidative cyclization of Mg-protoporphyrin IX monomethylester. |
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| Journal |
J. Bacteriol. 184 (2002) 746-53. |
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| Reference |
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| Authors |
Tottey S, Block MA, Allen M, Westergren T, Albrieux C, Scheller HV, Merchant S, Jensen PE |
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| Title |
Arabidopsis CHL27, located in both envelope and thylakoid membranes, is required for the synthesis of protochlorophyllide. |
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| Journal |
Proc. Natl. Acad. Sci. U. S. A. 100 (2003) 16119-24. |
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| Sequence |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: CAS: 92353-62-3 |
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| LinkDB |
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