KEGG   ORTHOLOGY: K05797Help
Entry
K05797                      KO                                     

Name
pchF
Definition
4-cresol dehydrogenase (hydroxylating) flavoprotein subunit [EC:1.17.99.1]
Pathway
Toluene degradation
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Xenobiotics biodegradation and metabolism
   00623 Toluene degradation
    K05797  pchF; 4-cresol dehydrogenase (hydroxylating) flavoprotein subunit
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.17  Acting on CH or CH2 groups
   1.17.99  With unknown physiological acceptors
    1.17.99.1  4-methylphenol dehydrogenase (hydroxylating)
     K05797  pchF; 4-cresol dehydrogenase (hydroxylating) flavoprotein subunit
BRITE hierarchy
Other DBs
Genes
PCQ: 
PcP3B5_13510(pchF_1) PcP3B5_26440(pchF_2) PcP3B5_26540(pchF_3)
PPUT: 
PDR: 
PALK: 
PSOS: 
POS17_3788(Neut_0635) POS17_5141(pchF)
PAT: 
AMB: 
CYQ: 
CZA: 
NOC: 
NHL: 
Nhal_1759
NWA: 
Nwat_1784
TMB: 
Thimo_2585
MPUR: 
MARPU_05365
HAK: 
KO116_00403
OCE: 
GU3_14310
GBI: 
PG2T_11235 PG2T_11275
BUG: 
BC1001_1419
BGE: 
BC1002_1210
BGF: 
BC1003_1960
BYI: 
BYI23_B003260(pchF)
BUO: 
BRPE64_BCDS09980
BUE: 
BRPE67_BCDS05760(pchF)
BUQ: 
AC233_09285
BXE: 
Bxe_A2781
BXB: 
DR64_464(pchF)
BPH: 
Bphy_1706
BPX: 
BUPH_01934
BPY: 
Bphyt_1669
BFN: 
OI25_3960(pchF)
BCAI: 
K788_0002432
PSPW: 
BJG93_06750
AXY: 
AXYL_04709
AIS: 
BUW96_16125
PNA: 
Pnap_3838
CTT: 
CtCNB1_1149
CKE: 
B5M06_10280
MNR: 
ACZ75_05905
NEU: 
NE0741
NET: 
Neut_1656
NIT: 
NAL212_2695
NCO: 
AAW31_08160
NUR: 
ATY38_06915
NMU: 
Nmul_A1475
NLC: 
EBAPG3_008560
EBA: 
ebA3165(pchF) ebA5380
RBU: 
PG1C_11260
AZA: 
AZKH_2917
AZI: 
AzCIB_2865
TMZ: 
Tmz1t_1840
THU: 
AC731_016445
TCL: 
Tchl_1574 Tchl_1580 Tchl_1596
SKU: 
Sulku_2563
CCOL: 
BN865_03820
CCF: 
YSQ_00025
CCOF: 
VC76_00030(pchF_2)
CCOO: 
ATE51_00010(pchF)
CURE: 
CUREO_0963
GME: 
Gmet_2125(pcmI)
GEO: 
Geob_0119(pcmI)
GEM: 
GM21_2847
CCX: 
COCOR_00350(pchF)
SUR: 
STAUR_5202
AGE: 
AA314_01948
CCRO: 
CMC5_053350
MRM: 
A7982_07674
DTI: 
Desti_5508
NAR: 
Saro_3479 Saro_3875
NPP: 
PP1Y_AT11456 PP1Y_AT15458 PP1Y_AT15495 PP1Y_AT31786 PP1Y_AT31825
NPN: 
JI59_21370
SSY: 
SLG_11340 SLG_11370 SLG_27820 SLG_38080
SPHB: 
EP837_03814
SPHR: 
BSY17_949
SPHG: 
AZE99_04610
ERY: 
CP97_14095 CP97_14115
AAY: 
WYH_02883(pchF_1) WYH_03108(pchF_2) WYH_03116(pchF_3) WYH_03119(pchF_4)
RRU: 
Rru_A3112
RRF: 
F11_15945
MAGQ: 
MGMAQ_0999
RHA: 
RHA1_ro03282
ROA: 
Pd630_LPD07539
SALS: 
SLNWT_0108
SGU: 
SGLAU_25455
SRN: 
A4G23_02275(pchF)
STRD: 
NI25_07845
MIO: 
AOA12_16300
CPHY: 
B5808_01085
ARL: 
AFL94_17620
NOA: 
BKM31_24930
MMAR: 
MODMU_2955(vaoA)
ACTN: 
L083_7156
MPK: 
VL20_5235
CSN: 
Cyast_1815
NOP: 
Nos7524_0706
PLP: 
Ple7327_1082
SUS: 
Acid_2038
FLI: 
Fleli_2307
NDE: 
NIDE0022(pchF)
AG: 
AGO01130(pchF)
 » show all
TaxonomyKoalaUniProt
Reference
PMID:7929007
  Authors
Kim J, Fuller JH, Cecchini G, McIntire WS
  Title
Cloning, sequencing, and expression of the structural genes for the cytochrome and flavoprotein subunits of p-cresol methylhydroxylase from two strains of Pseudomonas putida.
  Journal
J Bacteriol 176:6349-61 (1994)
DOI:10.1128/JB.176.20.6349-6361.1994
Reference
  Authors
Chen YF, Chao H, Zhou NY
  Title
The catabolism of 2,4-xylenol and p-cresol share the enzymes for the oxidation of para-methyl group in Pseudomonas putida NCIMB 9866.
  Journal
Appl Microbiol Biotechnol 98:1349-56 (2014)
DOI:10.1007/s00253-013-5001-z
  Sequence
LinkDB All DBs

KEGG   ORTHOLOGY: K20200Help
Entry
K20200                      KO                                     

Name
pchC
Definition
4-cresol dehydrogenase [hydroxylating] cytochrome subunit
Pathway
Toluene degradation
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Xenobiotics biodegradation and metabolism
   00623 Toluene degradation
    K20200  pchC; 4-cresol dehydrogenase [hydroxylating] cytochrome subunit
BRITE hierarchy
Other DBs
Genes
PPUT: 
PDR: 
PALK: 
PSOS: 
OCE: 
EBA: 
ebB97(pchC)
TaxonomyKoalaUniProt
Reference
PMID:7929007
  Authors
Kim J, Fuller JH, Cecchini G, McIntire WS
  Title
Cloning, sequencing, and expression of the structural genes for the cytochrome and flavoprotein subunits of p-cresol methylhydroxylase from two strains of Pseudomonas putida.
  Journal
J Bacteriol 176:6349-61 (1994)
DOI:10.1128/JB.176.20.6349-6361.1994
  Sequence
LinkDB All DBs

KEGG   ENZYME: 1.17.99.1Help
Entry
EC 1.17.99.1                Enzyme                                 

Name
4-methylphenol dehydrogenase (hydroxylating);
p-cresol-(acceptor) oxidoreductase (hydroxylating);
p-cresol methylhydroxylase;
4-cresol dehydrogenase (hydroxylating)
Class
Oxidoreductases;
Acting on CH or CH2 groups;
With unknown physiological acceptors
BRITE hierarchy
Sysname
4-methylphenol:acceptor oxidoreductase (methyl-hydroxylating)
Reaction(IUBMB)
4-methylphenol + 2 acceptor + H2O = 4-hydroxybenzaldehyde + 2 reduced acceptor [RN:R07223]
Reaction(KEGG)
R07223 > R02675;
(other) R11194
Show
Substrate
4-methylphenol [CPD:C01468];
acceptor [CPD:C00028];
H2O [CPD:C00001]
Product
4-hydroxybenzaldehyde [CPD:C00633];
reduced acceptor [CPD:C00030]
Comment
A flavocytochrome c (FAD). Phenazine methosulfate can act as acceptor. A quinone methide is probably formed as intermediate. The first hydroxylation forms 4-hydroxybenzyl alcohol; a second hydroxylation converts this into 4-hydroxybenzaldehyde.
History
EC 1.17.99.1 created 1983, modified 2001, modified 2011, modified 2015
Pathway
Toluene degradation
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K05797  
4-cresol dehydrogenase (hydroxylating) flavoprotein subunit
Genes
PCQ: 
PcP3B5_13510(pchF_1) PcP3B5_26440(pchF_2) PcP3B5_26540(pchF_3)
PPUT: 
PDR: 
PALK: 
PSOS: 
POS17_3788(Neut_0635) POS17_5141(pchF)
PAT: 
AMB: 
CYQ: 
CZA: 
NOC: 
NHL: 
Nhal_1759
NWA: 
Nwat_1784
TMB: 
Thimo_2585
MPUR: 
MARPU_05365
HAK: 
KO116_00403
OCE: 
GU3_14310
GBI: 
PG2T_11235 PG2T_11275
BUG: 
BC1001_1419
BGE: 
BC1002_1210
BGF: 
BC1003_1960
BYI: 
BYI23_B003260(pchF)
BUO: 
BRPE64_BCDS09980
BUE: 
BRPE67_BCDS05760(pchF)
BUQ: 
AC233_09285
BXE: 
Bxe_A2781
BXB: 
DR64_464(pchF)
BPH: 
Bphy_1706
BPX: 
BUPH_01934
BPY: 
Bphyt_1669
BFN: 
OI25_3960(pchF)
BCAI: 
K788_0002432
PSPW: 
BJG93_06750
AXY: 
AXYL_04709
AIS: 
BUW96_16125
PNA: 
Pnap_3838
CTT: 
CtCNB1_1149
CKE: 
B5M06_10280
MNR: 
ACZ75_05905
NEU: 
NE0741
NET: 
Neut_1656
NIT: 
NAL212_2695
NCO: 
AAW31_08160
NUR: 
ATY38_06915
NMU: 
Nmul_A1475
NLC: 
EBAPG3_008560
EBA: 
ebA3165(pchF) ebA5380
RBU: 
PG1C_11260
AZA: 
AZKH_2917
AZI: 
AzCIB_2865
TMZ: 
Tmz1t_1840
THU: 
AC731_016445
TCL: 
Tchl_1574 Tchl_1580 Tchl_1596
SKU: 
Sulku_2563
CCOL: 
BN865_03820
CCF: 
YSQ_00025
CCOF: 
VC76_00030(pchF_2)
CCOO: 
ATE51_00010(pchF)
CURE: 
CUREO_0963
GME: 
Gmet_2125(pcmI)
GEO: 
Geob_0119(pcmI)
GEM: 
GM21_2847
CCX: 
COCOR_00350(pchF)
SUR: 
STAUR_5202
AGE: 
AA314_01948
CCRO: 
CMC5_053350
MRM: 
A7982_07674
DTI: 
Desti_5508
NAR: 
Saro_3479 Saro_3875
NPP: 
PP1Y_AT11456 PP1Y_AT15458 PP1Y_AT15495 PP1Y_AT31786 PP1Y_AT31825
NPN: 
JI59_21370
SSY: 
SLG_11340 SLG_11370 SLG_27820 SLG_38080
SPHB: 
EP837_03814
SPHR: 
BSY17_949
SPHG: 
AZE99_04610
ERY: 
CP97_14095 CP97_14115
AAY: 
WYH_02883(pchF_1) WYH_03108(pchF_2) WYH_03116(pchF_3) WYH_03119(pchF_4)
RRU: 
Rru_A3112
RRF: 
F11_15945
MAGQ: 
MGMAQ_0999
RHA: 
RHA1_ro03282
ROA: 
Pd630_LPD07539
SALS: 
SLNWT_0108
SGU: 
SGLAU_25455
SRN: 
A4G23_02275(pchF)
STRD: 
NI25_07845
MIO: 
AOA12_16300
CPHY: 
B5808_01085
ARL: 
AFL94_17620
NOA: 
BKM31_24930
MMAR: 
MODMU_2955(vaoA)
ACTN: 
L083_7156
MPK: 
VL20_5235
CSN: 
Cyast_1815
NOP: 
Nos7524_0706
PLP: 
Ple7327_1082
SUS: 
Acid_2038
FLI: 
Fleli_2307
NDE: 
NIDE0022(pchF)
 » show all
Taxonomy
Reference
1  [PMID:588247]
  Authors
Hopper DJ, Taylor DG.
  Title
The purification and properties of p-cresol-(acceptor) oxidoreductase (hydroxylating), a flavocytochrome from Pseudomonas putida.
  Journal
Biochem. J. 167 (1977) 155-62.
Reference
2  [PMID:7391034]
  Authors
McIntire W, Edmondson DE, Singer TP, Hopper DJ.
  Title
8 alpha-O-Tyrosyl-FAD: a new form of covalently bound flavin from p-cresol methylhydroxylase.
  Journal
J. Biol. Chem. 255 (1980) 6553-5.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
66772-07-4
LinkDB All DBs

KEGG   REACTION: R02675Help
Entry
R02675                      Reaction                               

Name
4-cresol:acceptor oxidoreductase (methyl-hydroxylating)
Definition
4-Cresol + 2 Oxidized ferredoxin + H2O <=> 4-Hydroxybenzaldehyde + 2 Reduced ferredoxin
Equation
Comment
multi-step reaction
possibly intermediates (quinone methide and 4-hydroxybenzyl alcohol)
Reaction class
C00633_C01468
Enzyme
Pathway
Toluene degradation
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K05797  
4-cresol dehydrogenase (hydroxylating) flavoprotein subunit [EC:1.17.99.1]
K20200  
4-cresol dehydrogenase [hydroxylating] cytochrome subunit
LinkDB All DBs

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