KEGG   ORTHOLOGY: K13027Help
Entry
K13027                      KO                                     

Name
CYP79A1
Definition
tyrosine N-monooxygenase [EC:1.14.14.36]
Pathway
Cyanoamino acid metabolism
Glucosinolate biosynthesis
2-Oxocarboxylic acid metabolism
Module
M00369  
Cyanogenic glycoside biosynthesis, tyrosine => dhurrin
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Overview
   01210 2-Oxocarboxylic acid metabolism
    K13027  CYP79A1; tyrosine N-monooxygenase
  Metabolism of other amino acids
   00460 Cyanoamino acid metabolism
    K13027  CYP79A1; tyrosine N-monooxygenase
  Biosynthesis of other secondary metabolites
   00966 Glucosinolate biosynthesis
    K13027  CYP79A1; tyrosine N-monooxygenase
KEGG modules [BR:ko00002]
 Pathway module
  Secondary metabolism
   Biosynthesis of secondary metabolites
    M00369  Cyanogenic glycoside biosynthesis, tyrosine => dhurrin
     K13027  CYP79A1; tyrosine N-monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.36  tyrosine N-monooxygenase
     K13027  CYP79A1; tyrosine N-monooxygenase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, plant type
  CYP79 family
   K13027  CYP79A1; tyrosine N-monooxygenase
BRITE hierarchy
Other DBs
Genes
BDI: 
SBI: 
ZMA: 
SITA: 
TaxonomyKoalaUniProt
Reference
PMID:7487064
  Authors
Koch BM, Sibbesen O, Halkier BA, Svendsen I, Moller BL
  Title
The primary sequence of cytochrome P450tyr, the multifunctional N-hydroxylase catalyzing the conversion of L-tyrosine to p-hydroxyphenylacetaldehyde oxime in the biosynthesis of the cyanogenic glucoside dhurrin in Sorghum bicolor (L.) Moench.
  Journal
Arch Biochem Biophys 323:177-86 (1995)
DOI:10.1006/abbi.1995.0024
  Sequence
LinkDB All DBs

KEGG   ORTHOLOGY: K11818Help
Entry
K11818            Tight     KO                                     

Name
CYP83B1
Definition
cytochrome P450 family 83 subfamily B polypeptide 1 [EC:1.14.-.-]
Pathway
Tryptophan metabolism
Glucosinolate biosynthesis
2-Oxocarboxylic acid metabolism
Module
M00370  
Glucosinolate biosynthesis, tryptophan => glucobrassicin
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Overview
   01210 2-Oxocarboxylic acid metabolism
    K11818  CYP83B1; cytochrome P450 family 83 subfamily B polypeptide 1
  Amino acid metabolism
   00380 Tryptophan metabolism
    K11818  CYP83B1; cytochrome P450 family 83 subfamily B polypeptide 1
  Biosynthesis of other secondary metabolites
   00966 Glucosinolate biosynthesis
    K11818  CYP83B1; cytochrome P450 family 83 subfamily B polypeptide 1
KEGG modules [BR:ko00002]
 Pathway module
  Secondary metabolism
   Biosynthesis of secondary metabolites
    M00370  Glucosinolate biosynthesis, tryptophan => glucobrassicin
     K11818  CYP83B1; cytochrome P450 family 83 subfamily B polypeptide 1
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.-  Acting on paired donors, with incorporation or reduction of molecular oxygen
    1.14.-.-  
     K11818  CYP83B1; cytochrome P450 family 83 subfamily B polypeptide 1
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, plant type
  CYP83 family
   K11818  CYP83B1; cytochrome P450 family 83 subfamily B polypeptide 1
BRITE hierarchy
Other DBs
Genes
ATH: 
AT4G31500(CYP83B1)
ALY: 
CRB: 
EUS: 
BRP: 
103833295(CYP83B1)
BNA: 
106361266 106372082(CYP83B1-5)
TaxonomyKoalaUniProt
Reference
  Authors
Naur P, Petersen BL, Mikkelsen MD, Bak S, Rasmussen H, Olsen CE, Halkier BA
  Title
CYP83A1 and CYP83B1, two nonredundant cytochrome P450 enzymes metabolizing oximes in the biosynthesis of glucosinolates in Arabidopsis.
  Journal
Plant Physiol 133:63-72 (2003)
DOI:10.1104/pp.102.019240
LinkDB All DBs

KEGG   ORTHOLOGY: K11819Help
Entry
K11819                      KO                                     

Name
SUR1, RTY
Definition
S-alkyl-thiohydroximate lyase SUR1 [EC:4.4.1.-]
Pathway
Tryptophan metabolism
Glucosinolate biosynthesis
2-Oxocarboxylic acid metabolism
Module
M00370  
Glucosinolate biosynthesis, tryptophan => glucobrassicin
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Overview
   01210 2-Oxocarboxylic acid metabolism
    K11819  SUR1, RTY; S-alkyl-thiohydroximate lyase SUR1
  Amino acid metabolism
   00380 Tryptophan metabolism
    K11819  SUR1, RTY; S-alkyl-thiohydroximate lyase SUR1
  Biosynthesis of other secondary metabolites
   00966 Glucosinolate biosynthesis
    K11819  SUR1, RTY; S-alkyl-thiohydroximate lyase SUR1
KEGG modules [BR:ko00002]
 Pathway module
  Secondary metabolism
   Biosynthesis of secondary metabolites
    M00370  Glucosinolate biosynthesis, tryptophan => glucobrassicin
     K11819  SUR1, RTY; S-alkyl-thiohydroximate lyase SUR1
Enzymes [BR:ko01000]
 4. Lyases
  4.4  Carbon-sulfur lyases
   4.4.1  Carbon-sulfur lyases (only sub-subclass identified to date)
    4.4.1.-  
     K11819  SUR1, RTY; S-alkyl-thiohydroximate lyase SUR1
Amino acid related enzymes [BR:ko01007]
 Aminotransferase (transaminase)
  Others
   K11819  SUR1, RTY; S-alkyl-thiohydroximate lyase SUR1
BRITE hierarchy
Other DBs
Genes
ATH: 
AT2G20610(SUR1)
ALY: 
CRB: 
EUS: 
BRP: 
BNA: 
TaxonomyKoalaUniProt
LinkDB All DBs

KEGG   ENZYME: 2.4.1.195Help
Entry
EC 2.4.1.195                Enzyme                                 

Name
N-hydroxythioamide S-beta-glucosyltransferase;
desulfoglucosinolate-uridine diphosphate glucosyltransferase;
uridine diphosphoglucose-thiohydroximate glucosyltransferase;
thiohydroximate beta-D-glucosyltransferase;
UDPG:thiohydroximate glucosyltransferase;
thiohydroximate S-glucosyltransferase;
thiohydroximate glucosyltransferase;
UDP-glucose:thiohydroximate S-beta-D-glucosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-glucose:N-hydroxy-2-phenylethanethioamide S-beta-D-glucosyltransferase
Reaction(IUBMB)
UDP-glucose + N-hydroxy-2-phenylethanethioamide = UDP + desulfoglucotropeolin [RN:R03213]
Reaction(KEGG)
Substrate
UDP-glucose [CPD:C00029];
N-hydroxy-2-phenylethanethioamide
Product
UDP [CPD:C00015];
desulfoglucotropeolin [CPD:C01069]
Comment
Involved with EC 2.8.2.24, desulfoglucosinolate sulfotransferase, in the biosynthesis of thioglucosides in cruciferous plants.
History
EC 2.4.1.195 created 1992, modified 2006
Pathway
Tryptophan metabolism
Glucosinolate biosynthesis
Biosynthesis of secondary metabolites
Orthology
K11820  
N-hydroxythioamide S-beta-glucosyltransferase
Genes
RSS: 
ATH: 
AT1G24100(UGT74B1)
ALY: 
CRB: 
EUS: 
BRP: 
103840711(UGT74B1-1)
BNA: 
THJ: 
CIT: 
CIC: 
TCC: 
18603841 18609376
GRA: 
105766111 105785553 105789117
GHI: 
107903698 107916109 107922512 107957313 107963785
EGR: 
104427824 104432928
GMX: 
100787855 100805508 100806069
PVU: 
PHAVU_002G075200g
VRA: 
106754697
VAR: 
108334879
CCAJ: 
109800656 109801468
MTR: 
MTR_5g035560 MTR_5g035580
CAM: 
101500102
ADU: 
107488943 107491373
AIP: 
107643497 107643933
LJA: 
Lj2g3v1371330.1(Lj2g3v1371330.1)
LANG: 
109330572 109332223
FVE: 
101294357 101298891 101308673
PPER: 
18776421 18794038
PMUM: 
103338304 103343266
MDM: 
103425490 103455316
ZJU: 
107418463 107419884 107419915 107419916
RCU: 
8270386
JCU: 
105640428 105649838
POP: 
POPTR_0015s08330g(POPTRDRAFT_666418)
VVI: 
100260341 100260432
SLY: 
101251379
SPEN: 
107019146 107019181
SOT: 
102603088 102603422
INI: 
109154647 109154662
SIND: 
105168211
BVG: 
104894392 104905872 104908451
BDI: 
100835161
ATR: 
18448709
 » show all
Taxonomy
Reference
1  [PMID:2524977]
  Authors
Jain JC, Reed DW, GrootWassink JW, Underhill EW.
  Title
A radioassay of enzymes catalyzing the glucosylation and sulfation steps of glucosinolate biosynthesis in Brassica species.
  Journal
Anal. Biochem. 178 (1989) 137-40.
Reference
2  [PMID:8373190]
  Authors
Reed DW, Davin L, Jain JC, Deluca V, Nelson L, Underhill EW.
  Title
Purification and properties of UDP-glucose:thiohydroximate glucosyltransferase from Brassica napus L. seedlings.
  Journal
Arch. Biochem. Biophys. 305 (1993) 526-32.
Reference
3  [PMID:11198818]
  Authors
Fahey JW, Zalcmann AT, Talalay P.
  Title
The chemical diversity and distribution of glucosinolates and isothiocyanates among plants.
  Journal
Phytochemistry. 56 (2001) 5-51.
Reference
4
  Authors
Grubb, C.D., Zipp, B.J., Ludwig-Muller, J., Masuno, M.N., Molinski, T.F. and Abel, S.
  Title
Arabidopsis glucosyltransferase UGT74B1 functions in glucosinolate biosynthesis and auxin homeostasis.
  Journal
Plant J. 40 (2004) 893-908.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9068-14-8
LinkDB All DBs

KEGG   ORTHOLOGY: K11821Help
Entry
K11821            Tight     KO                                     

Name
ST5A_B_C
Definition
desulfoglucosinolate sulfotransferase A/B/C [EC:2.8.2.-]
Pathway
Tryptophan metabolism
Glucosinolate biosynthesis
2-Oxocarboxylic acid metabolism
Module
M00370  
Glucosinolate biosynthesis, tryptophan => glucobrassicin
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Overview
   01210 2-Oxocarboxylic acid metabolism
    K11821  ST5A_B_C; desulfoglucosinolate sulfotransferase A/B/C
  Amino acid metabolism
   00380 Tryptophan metabolism
    K11821  ST5A_B_C; desulfoglucosinolate sulfotransferase A/B/C
  Biosynthesis of other secondary metabolites
   00966 Glucosinolate biosynthesis
    K11821  ST5A_B_C; desulfoglucosinolate sulfotransferase A/B/C
KEGG modules [BR:ko00002]
 Pathway module
  Secondary metabolism
   Biosynthesis of secondary metabolites
    M00370  Glucosinolate biosynthesis, tryptophan => glucobrassicin
     K11821  ST5A_B_C; desulfoglucosinolate sulfotransferase A/B/C
Enzymes [BR:ko01000]
 2. Transferases
  2.8  Transferring sulfur-containing groups
   2.8.2  Sulfotransferases
    2.8.2.-  
     K11821  ST5A_B_C; desulfoglucosinolate sulfotransferase A/B/C
BRITE hierarchy
Other DBs
Genes
RSS: 
ATH: 
AT1G74100(SOT16)
ALY: 
CRB: 
EUS: 
BRP: 
103831884(ST5a-2) 103852881(ST5a)
BNA: 
TaxonomyKoalaUniProt
LinkDB All DBs

KEGG   REACTION: R10671Help
Entry
R10671                      Reaction                               

Name
L-tyrosine,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (N-hydroxylating)
Definition
L-Tyrosine + 2 Oxygen + 2 [Reduced NADPH---hemoprotein reductase] <=> (E)-4-Hydroxyphenylacetaldehyde oxime + 2 [Oxidized NADPH---hemoprotein reductase] + 3 H2O + CO2
Equation
C00082 + 2 C00007 + 2 C03024 <=> C04350 + 2 C03161 + 3 C00001 + C00011
Comment
three-step reaction (see R00730+R04460+R07190, similar to R09578, R09579+R09580+R09581)
Reaction class
C00082_C04350
Enzyme
Pathway
Glucosinolate biosynthesis
Biosynthesis of secondary metabolites
2-Oxocarboxylic acid metabolism
Orthology
K13027  
tyrosine N-monooxygenase [EC:1.14.14.36]
LinkDB All DBs

KEGG   REACTION: R10672Help
Entry
R10672                      Reaction                               

Name
4-hydroxyphenylacetaldehyde-oxime,NADPH:oxygen oxidoreductase (adding cysteine)
Definition
(E)-4-Hydroxyphenylacetaldehyde oxime + L-Cysteine + NADPH + Oxygen + H+ <=> S-(Hydroxyphenylacetothiohydroximoyl)-L-cysteine + NADP+ + 2 H2O
Equation
Comment
multi-step reaction (similar to R08168, R08607+R08608)
Reaction class
C00005_C00006
C00097_C17238
C04350_C17238
Enzyme
1.14.-.-
Pathway
Glucosinolate biosynthesis
Biosynthesis of secondary metabolites
2-Oxocarboxylic acid metabolism
Orthology
K11818  
cytochrome P450 family 83 subfamily B polypeptide 1 [EC:1.14.-.-]
LinkDB All DBs

KEGG   REACTION: R08660Help
Entry
R08660                      Reaction                               

Name
S-(hydroxyphenylacetothiohydroximoyl)-L-cysteine phenylacetothiohydroximate-lyase (deaminating; pyruvate-forming)
Definition
S-(Hydroxyphenylacetothiohydroximoyl)-L-cysteine + H2O <=> p-Hydroxyphenylacetothiohydroximate + Pyruvate + Ammonia
Equation
Reaction class
C00022_C17238
C17238_C17239
Enzyme
4.4.1.-
Pathway
Glucosinolate biosynthesis
Biosynthesis of secondary metabolites
2-Oxocarboxylic acid metabolism
Orthology
K11819  
S-alkyl-thiohydroximate lyase SUR1 [EC:4.4.1.-]
LinkDB All DBs

KEGG   REACTION: R08661Help
Entry
R08661                      Reaction                               

Name
UDP-glucose:p-hydroxyphenylacetothiohydroximate S-beta-D-glucosyltransferase
Definition
p-Hydroxyphenylacetothiohydroximate + UDP-glucose <=> p-Hydroxybenzyldesulphoglucosinolate + UDP
Equation
Reaction class
C00015_C00029
C17239_C17240
Enzyme
Pathway
Glucosinolate biosynthesis
Biosynthesis of secondary metabolites
2-Oxocarboxylic acid metabolism
LinkDB All DBs

KEGG   REACTION: R08662Help
Entry
R08662                      Reaction                               

Name
3'-phosphoadenylyl-sulfate:desulfo-p-hydroxybenzylglucosinolate sulfotransferase
Definition
p-Hydroxybenzyldesulphoglucosinolate + 3'-Phosphoadenylyl sulfate <=> Sinalbin + Adenosine 3',5'-bisphosphate
Equation
Reaction class
C00053_C00054
C08426_C17240
Enzyme
2.8.2.-
Pathway
Glucosinolate biosynthesis
Biosynthesis of secondary metabolites
2-Oxocarboxylic acid metabolism
Orthology
K11821  
desulfoglucosinolate sulfotransferase A/B/C [EC:2.8.2.-]
LinkDB All DBs

DBGET integrated database retrieval system