KEGG   ORTHOLOGY: K14190Help
Entry
K14190                      KO                                     

Name
VTC2_5
Definition
GDP-L-galactose phosphorylase [EC:2.7.7.69]
Pathway
Ascorbate and aldarate metabolism
Module
M00114  
Ascorbate biosynthesis, plants, glucose-6P => ascorbate
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Carbohydrate metabolism
   00053 Ascorbate and aldarate metabolism
    K14190  VTC2_5; GDP-L-galactose phosphorylase
KEGG modules [BR:ko00002]
 Pathway module
  Nucleotide and amino acid metabolism
   Cofactor and vitamin biosynthesis
    M00114  Ascorbate biosynthesis, plants, glucose-6P => ascorbate
     K14190  VTC2_5; GDP-L-galactose phosphorylase
Enzymes [BR:ko01000]
 2. Transferases
  2.7  Transferring phosphorus-containing groups
   2.7.7  Nucleotidyltransferases
    2.7.7.69  GDP-L-galactose phosphorylase
     K14190  VTC2_5; GDP-L-galactose phosphorylase
BRITE hierarchy
Other DBs
RN: 
Genes
RSS: 
ATH: 
AT4G26850(VTC2) AT5G55120(VTC5)
ALY: 
CRB: 
EUS: 
BRP: 
BNA: 
THJ: 
CIT: 
CIC: 
TCC: 
18593449 18601891
GRA: 
105768479 105781073 105782389
GHI: 
107903465 107917812 107919728 107921993 107932761
EGR: 
104423704 104423716 104423718 104448198 104450258 108953908 108954081
GMX: 
100785588 100800738 100806541
PVU: 
PHAVU_006G046700g PHAVU_008G269600g
VRA: 
106765047 106780161
VAR: 
108321941 108338475
CCAJ: 
109790922 109811290
MTR: 
MTR_3g053020 MTR_5g093390
CAM: 
101497835 101510212
ADU: 
107480942 107482709 107496337
AIP: 
107608900 107624932 107633751
LJA: 
Lj0g3v0129419.1(Lj0g3v0129419.1) Lj0g3v0268149.1(Lj0g3v0268149.1)
LANG: 
109330267 109338702 109361102
FVE: 
101304522 101307284 101314355
PPER: 
18778931
PMUM: 
103326528
MDM: 
103413187 103427292 103435625 103448375
PXB: 
103940897 103941667 103951401 103952371
ZJU: 
107416252 107416393 107432716
CSV: 
101205337
CMO: 
103490128
RCU: 
8262342 8263219 8287622
JCU: 
105633002 105638608
POP: 
POPTR_0001s36560g(POPTRDRAFT_642193) POPTR_0005s14440g POPTR_0011s08940g(POPTRDRAFT_660608)
VVI: 
100253859 100262099
SLY: 
101055572(GGP) 101255942
SPEN: 
107011245 107023056
SOT: 
102593176 102596542
INI: 
109162014 109177940
SIND: 
105174892 105176671 105177885
BVG: 
104891705 104906838
NNU: 
104588033 104588921 104594070 104601771 104609968
OSA: 
4351698
DOSA: 
Os12t0190000-01(Os12g0190000)
OBR: 
102720297 102721498
BDI: 
100833569 100842186 100843203
ATS: 
F775_19319 F775_27680 F775_32461
SBI: 
8084736
ZMA: 
100272546(si946084h12) 100283852
SITA: 
101757506 101780560
PDA: 
103700830 103700917 103710494 103711043 103717173 103720528
EGU: 
105033399 105036432 105037736 105038499 105041039 105046718 105048537
MUS: 
103987203 103987671 103987732 104000762
ATR: 
18433669
SMO: 
SELMODRAFT_105410 SELMODRAFT_110588 SELMODRAFT_139516 SELMODRAFT_427287 SELMODRAFT_439945 SELMODRAFT_99090
PPP: 
PHYPADRAFT_166416 PHYPADRAFT_23110 PHYPADRAFT_3338
CRE: 
CHLREDRAFT_113717
VCN: 
VOLCADRAFT_58668
OLU: 
OSTLU_3702
BPG: 
Bathy16g00370
MIS: 
MICPUN_64582
MPP: 
MICPUCDRAFT_63706
CSL: 
COCSUDRAFT_13851
CVR: 
CHLNCDRAFT_57139
APRO: 
F751_3621
TET: 
TTHERM_000683404
 » show all
TaxonomyKoalaUniProt
Reference
  Authors
Linster CL, Gomez TA, Christensen KC, Adler LN, Young BD, Brenner C, Clarke SG.
  Title
Arabidopsis VTC2 Encodes a GDP-L-Galactose Phosphorylase, the Last Unknown Enzyme in the Smirnoff-Wheeler Pathway to Ascorbic Acid in Plants.
  Journal
J Biol Chem 282:18879-85 (2007)
DOI:10.1074/jbc.M702094200
  Sequence
[ath:AT4G26850]
LinkDB All DBs

KEGG   ENZYME: 2.7.7.69Help
Entry
EC 2.7.7.69                 Enzyme                                 

Name
GDP-L-galactose phosphorylase;
VTC2;
VTC5
Class
Transferases;
Transferring phosphorus-containing groups;
Nucleotidyltransferases
BRITE hierarchy
Sysname
GDP:alpha-L-galactose 1-phosphate guanylyltransferase
Reaction(IUBMB)
GDP-beta-L-galactose + phosphate = beta-L-galactose 1-phosphate + GDP
Reaction(KEGG)
(other) R07678
Show
Substrate
GDP-beta-L-galactose;
phosphate [CPD:C00009]
Product
beta-L-galactose 1-phosphate [CPD:C15926];
GDP [CPD:C00035]
Comment
The enzyme catalyses a reaction of the Smirnoff-Wheeler pathway, the major route to ascorbate biosynthesis in plants.
History
EC 2.7.7.69 created 2010
Pathway
Ascorbate and aldarate metabolism
Metabolic pathways
Biosynthesis of secondary metabolites
Orthology
K14190  
GDP-L-galactose phosphorylase
Genes
RSS: 
ATH: 
AT4G26850(VTC2) AT5G55120(VTC5)
ALY: 
CRB: 
EUS: 
BRP: 
BNA: 
THJ: 
CIT: 
CIC: 
TCC: 
18593449 18601891
GRA: 
105768479 105781073 105782389
GHI: 
107903465 107917812 107919728 107921993 107932761
EGR: 
104423704 104423716 104423718 104448198 104450258 108953908 108954081
GMX: 
100785588 100800738 100806541
PVU: 
PHAVU_006G046700g PHAVU_008G269600g
VRA: 
106765047 106780161
VAR: 
108321941 108338475
CCAJ: 
109790922 109811290
MTR: 
MTR_3g053020 MTR_5g093390
CAM: 
101497835 101510212
ADU: 
107480942 107482709 107496337
AIP: 
107608900 107624932 107633751
LJA: 
Lj0g3v0129419.1(Lj0g3v0129419.1) Lj0g3v0268149.1(Lj0g3v0268149.1)
LANG: 
109330267 109338702 109361102
FVE: 
101304522 101307284 101314355
PPER: 
18778931
PMUM: 
103326528
MDM: 
103413187 103427292 103435625 103448375
PXB: 
103940897 103941667 103951401 103952371
ZJU: 
107416252 107416393 107432716
CSV: 
101205337
CMO: 
103490128
RCU: 
8262342 8263219 8287622
JCU: 
105633002 105638608
POP: 
POPTR_0001s36560g(POPTRDRAFT_642193) POPTR_0005s14440g POPTR_0011s08940g(POPTRDRAFT_660608)
VVI: 
100253859 100262099
SLY: 
101055572(GGP) 101255942
SPEN: 
107011245 107023056
SOT: 
102593176 102596542
INI: 
109162014 109177940
SIND: 
105174892 105176671 105177885
BVG: 
104891705 104906838
NNU: 
104588033 104588921 104594070 104601771 104609968
OSA: 
4351698
DOSA: 
Os12t0190000-01(Os12g0190000)
OBR: 
102720297 102721498
BDI: 
100833569 100842186 100843203
ATS: 
F775_19319 F775_27680 F775_32461
SBI: 
8084736
ZMA: 
100272546(si946084h12) 100283852
SITA: 
101757506 101780560
PDA: 
103700830 103700917 103710494 103711043 103717173 103720528
EGU: 
105033399 105036432 105037736 105038499 105041039 105046718 105048537
MUS: 
103987203 103987671 103987732 104000762
ATR: 
18433669
SMO: 
SELMODRAFT_105410 SELMODRAFT_110588 SELMODRAFT_139516 SELMODRAFT_427287 SELMODRAFT_439945 SELMODRAFT_99090
PPP: 
PHYPADRAFT_166416 PHYPADRAFT_23110 PHYPADRAFT_3338
CRE: 
CHLREDRAFT_113717
VCN: 
VOLCADRAFT_58668
OLU: 
OSTLU_3702
BPG: 
Bathy16g00370
MIS: 
MICPUN_64582
MPP: 
MICPUCDRAFT_63706
CSL: 
COCSUDRAFT_13851
CVR: 
CHLNCDRAFT_57139
APRO: 
F751_3621
TET: 
TTHERM_000683404
 » show all
Taxonomy
Reference
1  [PMID:17462988]
  Authors
Linster CL, Gomez TA, Christensen KC, Adler LN, Young BD, Brenner C, Clarke SG.
  Title
Arabidopsis VTC2 Encodes a GDP-L-Galactose Phosphorylase, the Last Unknown Enzyme in the Smirnoff-Wheeler Pathway to Ascorbic Acid in Plants.
  Journal
J. Biol. Chem. 282 (2007) 18879-85.
  Sequence
[ath:AT4G26850]
Reference
2  [PMID:18463094]
  Authors
Linster CL, Adler LN, Webb K, Christensen KC, Brenner C, Clarke SG
  Title
A second GDP-L-galactose phosphorylase in arabidopsis en route to vitamin C. Covalent intermediate and substrate requirements for the conserved reaction.
  Journal
J. Biol. Chem. 283 (2008) 18483-92.
  Sequence
Reference
3  [PMID:17877701]
  Authors
Dowdle J, Ishikawa T, Gatzek S, Rolinski S, Smirnoff N
  Title
Two genes in Arabidopsis thaliana encoding GDP-L-galactose phosphorylase are required for ascorbate biosynthesis and seedling viability.
  Journal
Plant. J. 52 (2007) 673-89.
  Sequence
Reference
4  [PMID:18516687]
  Authors
Muller-Moule P
  Title
An expression analysis of the ascorbate biosynthesis enzyme VTC2.
  Journal
Plant. Mol. Biol. 68 (2008) 31-41.
  Sequence
[ath:AT4G26850]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
LinkDB All DBs

KEGG   REACTION: R07678Help
Entry
R07678                      Reaction                               

Definition
GDP-L-galactose + Orthophosphate <=> beta-L-Galactose 1-phosphate + GDP
Equation
Reaction class
C02280_C15926
Enzyme
Pathway
Ascorbate and aldarate metabolism
Metabolic pathways
Biosynthesis of secondary metabolites
Module
M00114  
Ascorbate biosynthesis, plants, glucose-6P => ascorbate
Orthology
K14190  
GDP-L-galactose phosphorylase [EC:2.7.7.69]
LinkDB All DBs

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