KEGG   ORTHOLOGY: K14534Help
Entry
K14534                      KO                                     

Name
abfD
Definition
4-hydroxybutyryl-CoA dehydratase / vinylacetyl-CoA-Delta-isomerase [EC:4.2.1.120 5.3.3.3]
Pathway
Butanoate metabolism
Carbon fixation pathways in prokaryotes
Carbon metabolism
Module
M00374  
Dicarboxylate-hydroxybutyrate cycle
M00375  
Hydroxypropionate-hydroxybutylate cycle
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Overview
   01200 Carbon metabolism
    K14534  abfD; 4-hydroxybutyryl-CoA dehydratase / vinylacetyl-CoA-Delta-isomerase
  Carbohydrate metabolism
   00650 Butanoate metabolism
    K14534  abfD; 4-hydroxybutyryl-CoA dehydratase / vinylacetyl-CoA-Delta-isomerase
  Energy metabolism
   00720 Carbon fixation pathways in prokaryotes
    K14534  abfD; 4-hydroxybutyryl-CoA dehydratase / vinylacetyl-CoA-Delta-isomerase
KEGG modules [BR:ko00002]
 Pathway module
  Energy metabolism
   Carbon fixation
    M00375  Hydroxypropionate-hydroxybutylate cycle
     K14534  abfD; 4-hydroxybutyryl-CoA dehydratase / vinylacetyl-CoA-Delta-isomerase
    M00374  Dicarboxylate-hydroxybutyrate cycle
     K14534  abfD; 4-hydroxybutyryl-CoA dehydratase / vinylacetyl-CoA-Delta-isomerase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.120  4-hydroxybutanoyl-CoA dehydratase
     K14534  abfD; 4-hydroxybutyryl-CoA dehydratase / vinylacetyl-CoA-Delta-isomerase
 5. Isomerases
  5.3  Intramolecular oxidoreductases
   5.3.3  Transposing C=C bonds
    5.3.3.3  vinylacetyl-CoA Delta-isomerase
     K14534  abfD; 4-hydroxybutyryl-CoA dehydratase / vinylacetyl-CoA-Delta-isomerase
BRITE hierarchy
Other DBs
Genes
PNU: 
VPE: 
VPD: 
VAA: 
RTA: 
HYB: 
MPT: 
LCH: 
METR: 
APP: 
GME: 
Gmet_2215
DEU: 
DBW_0157(abfD)
DOL: 
Dole_2592
DAL: 
Dalk_1215
DTO: 
TOL2_C28460(hpaB)
SAMY: 
DB32_005276
SAT: 
SYN_02445
DTI: 
Desti_0809 Desti_1603 Desti_2004 Desti_3009 Desti_3036
SFU: 
Sfum_3141
DBR: 
Deba_0626
DAV: 
DESACE_06790
OCA: 
OCAR_6245
OCG: 
OCA5_c17860(hbaB)
OCO: 
OCA4_c17860(hbaB)
RHZ: 
RHPLAN_22730
SMAG: 
AN936_12040
SMAZ: 
LH19_13280
SPHQ: 
BWQ93_08330
BACO: 
OXB_3219
CBE: 
Cbei_2100
CBZ: 
Cbs_2100
CBEI: 
LF65_02412
CKL: 
CKL_3020(abfD)
CKR: 
CKR_2668
CLS: 
CXIVA_14640
CPAS: 
Clopa_4009
CSQ: 
CSCA_3616 CSCA_3622
CCK: 
Ccar_01580
CTYK: 
CTK_C07640(abfD)
CEU: 
A7L45_11985
LACY: 
A4V08_31375
CDF: 
CD630_23410(abfD)
PDC: 
CDIF630_02580(abfD)
CDC: 
CD196_2184(abfD)
CDL: 
CDR20291_2230(abfD)
PDF: 
CD630DERM_23410(abfD)
SLP: 
Slip_0662
DDH: 
Desde_1768
DMT: 
DESME_04405
PTH: 
PTH_1222
DOR: 
Desor_0848 Desor_0856 Desor_0867 Desor_2541 Desor_3713
DAI: 
Desaci_1066 Desaci_1680
DMI: 
Desmer_0870 Desmer_3350
ELM: 
ELI_0281
OVA: 
OBV_01110(abfD) OBV_03760(abfD) OBV_28840(abfD) OBV_33370(abfD) OBV_35330(abfD) OBV_40730(abfD)
IBU: 
IB211_00283 IB211_01887c
FPLA: 
A4U99_00480
CHY: 
CHY_1314(abfD) CHY_1730
PED: 
ING2D1G_1210(abfD)
MED: 
MELS_0342
AIN: 
Acin_2034
MID: 
MIP_03610
TRM: 
JO41_12025
FNC: 
HMPREF0946_00150
FNT: 
HMPREF0405_01111
PGI: 
PG_0692(abfD)
PGN: 
PGN_0727
PGT: 
PGTDC60_1817(abfD)
PAH: 
Poras_0448
TFO: 
BFO_1484(abfD)
OSP: 
Odosp_2062
RSI: 
Runsl_1400
TTK: 
TST_0398(abfD)
AFU: 
AF_0333
AFG: 
AFULGI_00003320 AFULGI_00011230
AST: 
Asulf_01733
GAC: 
GACE_1499
GAH: 
GAH_01688
IHO: 
Igni_0595
IIS: 
EYM_00450
PFM: 
Pyrfu_1275
SSO: 
SSO2738(abfD-2)
SOL: 
Ssol_0550
SSOA: 
SULA_0541
SSOL: 
SULB_0543
SSOF: 
SULC_0541
STO: 
STK_16590
SAI: 
Saci_2143(abfD)
SACN: 
SacN8_10430
SACR: 
SacRon12I_10685
SACS: 
SUSAZ_09780
SIS: 
LS215_2744
SIA: 
M1425_2585
SIM: 
M1627_2638
SID: 
M164_2569
SIY: 
YG5714_2751
SIN: 
YN1551_0139
SII: 
LD85_2896
SIH: 
SiH_2522
SIR: 
SiRe_2362
SIC: 
SiL_2419
SULA: 
BFU36_11260
MSE: 
Msed_1321
MCN: 
Mcup_0888
AHO: 
Ahos_2036
AMAN: 
B6F84_01555
PAI: 
PAE2693
PIS: 
Pisl_0248
PCL: 
Pcal_1396
PYR: 
P186_0718
POG: 
Pogu_1298
TNE: 
Tneu_0422
PYW: 
PYWP30_00440
TUZ: 
TUZN_0949
TTN: 
TTX_1102
CLG: 
Calag_0024
NMR: 
Nmar_0207
NIR: 
NSED_00835
NID: 
NPIRD3C_0596(abfD)
NIN: 
NADRNF5_2050(abfD)
NKR: 
NKOR_00855
NCT: 
NMSP_1505
CSY: 
CENSYa_0022
NGA: 
Ngar_c34390
NVN: 
NVIE_017160
NEV: 
NTE_00645
TAA: 
NMY3_03315(abfD)
NBV: 
T478_1248(abfD)
TAH: 
SU86_007675
NDV: 
NDEV_0181(hcd)
BARC: 
AOA65_2031 AOA65_2222
LOKI: 
Lokiarch_00630(abfD_1) Lokiarch_26350(abfD_2)
AG: 
CAB60035(abfD)
 » show all
TaxonomyKoalaUniProt
Reference
PMID:8344309
  Authors
Scherf U, Buckel W
  Title
Purification and properties of an iron-sulfur and FAD-containing 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA delta 3-delta 2-isomerase from Clostridium aminobutyricum.
  Journal
Eur J Biochem 215:421-9 (1993)
DOI:10.1111/j.1432-1033.1993.tb18049.x
  Sequence
LinkDB All DBs

KEGG   ENZYME: 4.2.1.120Help
Entry
EC 4.2.1.120                Enzyme                                 

Name
4-hydroxybutanoyl-CoA dehydratase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
4-hydroxybutanoyl-CoA hydro-lyase
Reaction(IUBMB)
4-hydroxybutanoyl-CoA = (E)-but-2-enoyl-CoA + H2O [RN:R10782]
Reaction(KEGG)
Substrate
4-hydroxybutanoyl-CoA [CPD:C11062]
Product
(E)-but-2-enoyl-CoA [CPD:C00877];
H2O [CPD:C00001]
Comment
Contains FAD and a [4Fe-4S] iron-sulfur cluster. The enzyme has been characterized from several microorganisms, including Clostridium kluyveri, where it participates in succinate fermentation [1,2], Clostridium aminobutyricum, where it participates in 4-aminobutyrate degradation [3,4], and Metallosphaera sedula, where it participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea [5].
History
EC 4.2.1.120 created 2009
Pathway
Butanoate metabolism
Carbon fixation pathways in prokaryotes
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K14534  
4-hydroxybutyryl-CoA dehydratase / vinylacetyl-CoA-Delta-isomerase
Genes
PNU: 
VPE: 
VPD: 
VAA: 
RTA: 
HYB: 
MPT: 
LCH: 
METR: 
APP: 
GME: 
Gmet_2215
DEU: 
DBW_0157(abfD)
DOL: 
Dole_2592
DAL: 
Dalk_1215
DTO: 
TOL2_C28460(hpaB)
SAMY: 
DB32_005276
SAT: 
SYN_02445
DTI: 
Desti_0809 Desti_1603 Desti_2004 Desti_3009 Desti_3036
SFU: 
Sfum_3141
DBR: 
Deba_0626
DAV: 
DESACE_06790
OCA: 
OCAR_6245
OCG: 
OCA5_c17860(hbaB)
OCO: 
OCA4_c17860(hbaB)
RHZ: 
RHPLAN_22730
SMAG: 
AN936_12040
SMAZ: 
LH19_13280
SPHQ: 
BWQ93_08330
BACO: 
OXB_3219
CBE: 
Cbei_2100
CBZ: 
Cbs_2100
CBEI: 
LF65_02412
CKL: 
CKL_3020(abfD)
CKR: 
CKR_2668
CLS: 
CXIVA_14640
CPAS: 
Clopa_4009
CSQ: 
CSCA_3616 CSCA_3622
CCK: 
Ccar_01580
CTYK: 
CTK_C07640(abfD)
CEU: 
A7L45_11985
LACY: 
A4V08_31375
CDF: 
CD630_23410(abfD)
PDC: 
CDIF630_02580(abfD)
CDC: 
CD196_2184(abfD)
CDL: 
CDR20291_2230(abfD)
PDF: 
CD630DERM_23410(abfD)
SLP: 
Slip_0662
DDH: 
Desde_1768
DMT: 
DESME_04405
PTH: 
PTH_1222
DOR: 
Desor_0848 Desor_0856 Desor_0867 Desor_2541 Desor_3713
DAI: 
Desaci_1066 Desaci_1680
DMI: 
Desmer_0870 Desmer_3350
ELM: 
ELI_0281
OVA: 
OBV_01110(abfD) OBV_03760(abfD) OBV_28840(abfD) OBV_33370(abfD) OBV_35330(abfD) OBV_40730(abfD)
IBU: 
IB211_00283 IB211_01887c
FPLA: 
A4U99_00480
CHY: 
CHY_1314(abfD) CHY_1730
PED: 
ING2D1G_1210(abfD)
MED: 
MELS_0342
AIN: 
Acin_2034
MID: 
MIP_03610
TRM: 
JO41_12025
FNC: 
HMPREF0946_00150
FNT: 
HMPREF0405_01111
PGI: 
PG_0692(abfD)
PGN: 
PGN_0727
PGT: 
PGTDC60_1817(abfD)
PAH: 
Poras_0448
TFO: 
BFO_1484(abfD)
OSP: 
Odosp_2062
RSI: 
Runsl_1400
TTK: 
TST_0398(abfD)
AFU: 
AF_0333
AFG: 
AFULGI_00003320 AFULGI_00011230
AST: 
Asulf_01733
GAC: 
GACE_1499
GAH: 
GAH_01688
IHO: 
Igni_0595
IIS: 
EYM_00450
PFM: 
Pyrfu_1275
SSO: 
SSO2738(abfD-2)
SOL: 
Ssol_0550
SSOA: 
SULA_0541
SSOL: 
SULB_0543
SSOF: 
SULC_0541
STO: 
STK_16590
SAI: 
Saci_2143(abfD)
SACN: 
SacN8_10430
SACR: 
SacRon12I_10685
SACS: 
SUSAZ_09780
SIS: 
LS215_2744
SIA: 
M1425_2585
SIM: 
M1627_2638
SID: 
M164_2569
SIY: 
YG5714_2751
SIN: 
YN1551_0139
SII: 
LD85_2896
SIH: 
SiH_2522
SIR: 
SiRe_2362
SIC: 
SiL_2419
SULA: 
BFU36_11260
MSE: 
Msed_1321
MCN: 
Mcup_0888
AHO: 
Ahos_2036
AMAN: 
B6F84_01555
PAI: 
PAE2693
PIS: 
Pisl_0248
PCL: 
Pcal_1396
PYR: 
P186_0718
POG: 
Pogu_1298
TNE: 
Tneu_0422
PYW: 
PYWP30_00440
TUZ: 
TUZN_0949
TTN: 
TTX_1102
CLG: 
Calag_0024
NMR: 
Nmar_0207
NIR: 
NSED_00835
NID: 
NPIRD3C_0596(abfD)
NIN: 
NADRNF5_2050(abfD)
NKR: 
NKOR_00855
NCT: 
NMSP_1505
CSY: 
CENSYa_0022
NGA: 
Ngar_c34390
NVN: 
NVIE_017160
NEV: 
NTE_00645
TAA: 
NMY3_03315(abfD)
NBV: 
T478_1248(abfD)
TAH: 
SU86_007675
NDV: 
NDEV_0181(hcd)
BARC: 
AOA65_2031 AOA65_2222
LOKI: 
Lokiarch_00630(abfD_1) Lokiarch_26350(abfD_2)
 » show all
Taxonomy
Reference
1  [PMID:13687513]
  Authors
BARTSCH RG, BARKER HA
  Title
A vinylacetyl isomerase from Clostridium kluyveri.
  Journal
Arch. Biochem. Biophys. 92 (1961) 122-32.
Reference
2  [PMID:8161284]
  Authors
Scherf U, Sohling B, Gottschalk G, Linder D, Buckel W
  Title
Succinate-ethanol fermentation in Clostridium kluyveri: purification and characterisation of 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA delta 3-delta 2-isomerase.
  Journal
Arch. Microbiol. 161 (1994) 239-45.
  Sequence
[ckl:CKL_3020]
Reference
3  [PMID:8344309]
  Authors
Scherf U, Buckel W
  Title
Purification and properties of an iron-sulfur and FAD-containing 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA delta 3-delta 2-isomerase from Clostridium aminobutyricum.
  Journal
Eur. J. Biochem. 215 (1993) 421-9.
  Sequence
Reference
4  [PMID:8794752]
  Authors
Muh U, Cinkaya I, Albracht SP, Buckel W
  Title
4-Hydroxybutyryl-CoA dehydratase from Clostridium aminobutyricum: characterization of FAD and iron-sulfur clusters involved in an overall non-redox reaction.
  Journal
Biochemistry. 35 (1996) 11710-8.
Reference
5  [PMID:18079405]
  Authors
Berg IA, Kockelkorn D, Buckel W, Fuchs G
  Title
A 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea.
  Journal
Science. 318 (2007) 1782-6.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
LinkDB All DBs

KEGG   REACTION: R10782Help
Entry
R10782                      Reaction                               

Name
4-hydroxybutanoyl-CoA hydro-lyase
Definition
4-Hydroxybutyryl-CoA <=> Crotonoyl-CoA + H2O
Equation
Reaction class
C00877_C11062
Enzyme
Pathway
Butanoate metabolism
Carbon fixation pathways in prokaryotes
Metabolic pathways
Microbial metabolism in diverse environments
Carbon metabolism
Module
M00374  
Dicarboxylate-hydroxybutyrate cycle
M00375  
Hydroxypropionate-hydroxybutylate cycle
Orthology
K14534  
4-hydroxybutyryl-CoA dehydratase / vinylacetyl-CoA-Delta-isomerase [EC:4.2.1.120 5.3.3.3]
Other DBs
RHEA: 
LinkDB All DBs

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