2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoate hydrolase [EC:3.7.1.13]

PMID:20368802

Gai Z, Wang X, Liu X, Tai C, Tang H, He X, Wu G, Deng Z, Xu P

The genes coding for the conversion of carbazole to catechol are flanked by IS6100 elements in Sphingomonas sp. strain XLDN2-5.

PLoS One 5:e10018 (2010)
DOI:10.1371/journal.pone.0010018

Hydrolases;
Acting on carbon-carbon bonds;
In ketonic substances

(2E,4E)-6-(2-aminophenyl)-2-hydroxy-6-oxohexa-2,4-dienoate acylhydrolase

(2E,4E)-6-(2-aminophenyl)-2-hydroxy-6-oxohexa-2,4-dienoate + H2O = anthranilate + (2E)-2-hydroxypenta-2,4-dienoate

(other) R05365

(2E,4E)-6-(2-aminophenyl)-2-hydroxy-6-oxohexa-2,4-dienoate;
H2O [CPD:C00001]

This enzyme catalyses the third step in the aerobic degradation pathway of carbazole. The effect of the presence of an amino group or hydroxyl group at the 2-position of the substrate is small. The enzyme has no cofactor requirement [2].

EC 3.7.1.13 created 2010

1  [PMID:12619671]

Nojiri H, Taira H, Iwata K, Morii K, Nam JW, Yoshida T, Habe H, Nakamura S, Shimizu K, Yamane H, Omori T

Purification and characterization of meta-cleavage compound hydrolase from a carbazole degrader Pseudomonas resinovorans strain CA10.

Biosci. Biotechnol. Biochem. 67 (2003) 36-45.

[pre:PCA10_p0310]

2  [PMID:12651123]

Riddle RR, Gibbs PR, Willson RC, Benedik MJ

Purification and properties of 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase involved in microbial degradation of carbazole.

Protein. Expr. Purif. 28 (2003) 182-9.

[pre:PCA10_p0310]