(E)-2-methylbutanal oxime monooxygenase [EC:1.14.14.41]

PMID:13680228

Zhang P, Bohl-Zenger S, Puonti-Kaerlas J, Potrykus I, Gruissem W

Two cassava promoters related to vascular expression and storage root formation.

Planta 218:192-203 (2003)
DOI:10.1007/s00425-003-1098-0

[ag:AAP57704]

PMID:21736650

Kannangara R, Motawia MS, Hansen NK, Paquette SM, Olsen CE, Moller BL, Jorgensen K

Characterization and expression profile of two UDP-glucosyltransferases, UGT85K4  and UGT85K5, catalyzing the last step in cyanogenic glucoside biosynthesis in cassava.

Plant J 68:287-301 (2011)
DOI:10.1111/j.1365-313X.2011.04695.x

PMID:21045121

Jorgensen K, Morant AV, Morant M, Jensen NB, Olsen CE, Kannangara R, Motawia MS, Moller BL, Bak S

Biosynthesis of the cyanogenic glucosides linamarin and lotaustralin in cassava:  isolation, biochemical characterization, and expression pattern of CYP71E7, the oxime-metabolizing cytochrome P450 enzyme.

Plant Physiol 155:282-92 (2011)
DOI:10.1104/pp.110.164053

[ag:AAP57704]

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

(E)-2-methylbutanal oxime,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase

(1) (E)-2-methylbutanal oxime + [reduced NADPH---hemoprotein reductase] + O2 = 2-hydroxy-2-methylbutanenitrile + [oxidized NADPH---hemoprotein reductase] + 2 H2O (overall reaction) [RN:R11597];
(1a) (E)-2-methylbutanal oxime = (Z)-2-methylbutanal oxime [RN:R10030];
(1b) (Z)-2-methylbutanal oxime = 2-methylbutanenitrile + H2O [RN:R11639];
(1c) 2-methylbutanenitrile + [reduced NADPH---hemoprotein reductase] + O2 = 2-hydroxy-2-methylbutanenitrile + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R11640];
(2) (E)-2-methylpropanal oxime + [reduced NADPH---hemoprotein reductase] + O2 = 2-hydroxy-2-methylpropanenitrile + [oxidized NADPH---hemoprotein reductase] + 2 H2O (overall reaction) [RN:R11598];
(2a) (E)-2-methylpropanal oxime = (Z)-2-methylpropanal oxime [RN:R10034];
(2b) (Z)-2-methylpropanal oxime = 2-methylpropanenitrile + H2O [RN:R11641];
(2c) 2-methylpropanenitrile + [reduced NADPH---hemoprotein reductase] + O2 = 2-hydroxy-2-methylpropanenitrile + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R11642]

R10030 R10034 R11597 R11598 R11639 R11640 R11641 R11642

(E)-2-methylbutanal oxime [CPD:C19491];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
(Z)-2-methylbutanal oxime [CPD:C20312];
2-methylbutanenitrile [CPD:C21525];
(E)-2-methylpropanal oxime [CPD:C03219];
(Z)-2-methylpropanal oxime [CPD:C20315];
2-methylpropanenitrile [CPD:C02420]

This cytochrome P-450 (heme thiolate) enzyme is involved in the biosynthesis of the cyanogenic glucosides lotaustralin and linamarin. It catalyses three different activities - isomerization of its substrate, the (E) isomer, to the (Z) isomer, dehydration, and C-hydroxylation.

EC 1.14.14.41 created 2017

1  [PMID:21045121]

Jorgensen K, Morant AV, Morant M, Jensen NB, Olsen CE, Kannangara R, Motawia MS, Moller BL, Bak S

Biosynthesis of the cyanogenic glucosides linamarin and lotaustralin in cassava:  isolation, biochemical characterization, and expression pattern of CYP71E7, the oxime-metabolizing cytochrome P450 enzyme.

Plant. Physiol. 155 (2011) 282-92.

[ag:AAP57704]