| Entry |
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| Name |
tetrachloroethene reductive dehalogenase;
tetrachloroethene reductase
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| Class |
Oxidoreductases;
Catalysing the reaction X-H + Y-H = X-Y;
With unknown physiological acceptors
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| Sysname |
acceptor:trichloroethene oxidoreductase (chlorinating)
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| Reaction(IUBMB) |
trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor [RN: R05753]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
tetrachloroethene [CPD: C06789];
reduced acceptor [CPD: C00030]
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| Comment |
This enzyme allows the common pollutant tetrachloroethene to support bacterial growth and is responsible for disposal of a number of chlorinated hydrocarbons. The reaction occurs in the reverse direction. The enzyme also reduces trichloroethene to dichloroethene. Although the physiological reductant is unknown, the supply of reductant in some organisms involves menaquinol, which is reduced by molecular hydrogen via the action of EC 1.12.5.1, hydrogen:quinone oxidoreductase. The enzyme contains a corrinoid and two iron-sulfur clusters. Methyl viologen can act as electron donor in vitro.
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| History |
EC 1.21.99.5 created 2001 as EC 1.97.1.8, transferred 2017 to EC 1.21.99.5
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| Pathway |
| Chloroalkane and chloroalkene degradation | | Metabolic pathways | | Microbial metabolism in diverse environments |
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| Orthology |
| tetrachloroethene reductive dehalogenase |
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| Genes |
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| Reference |
1 |
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| Authors |
Holliger, C, Wohlfarth, G. and Diekert, G. |
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| Title |
Reductive dechlorination in the energy metabolism of anaerobic bacteria. |
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| Journal |
FEMS Microbiol. Rev. 22 (1998) 383-398. |
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| Reference |
2 |
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| Authors |
Glod, G., Angst, W., Holliger, C. and Schwarzenbach, R.P. |
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| Title |
Corrinoid-mediated reduction of tetrachloroethene, trichloroethene, and trichlorofluoroethene in homogeneous aqueous solution: Reaction kinetics and reaction mechanisms. |
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| Journal |
Environ. Sci. Technol. 31 (1997) 253-260. |
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| Reference |
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| Authors |
Neumann A, Wohlfarth G, Diekert G. |
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| Title |
Purification and characterization of tetrachloroethene reductive dehalogenase from Dehalospirillum multivorans. |
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| Journal |
J. Biol. Chem. 271 (1996) 16515-9. |
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| Reference |
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| Authors |
Schumacher W, Holliger C, Zehnder AJ, Hagen WR. |
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| Title |
Redox chemistry of cobalamin and iron-sulfur cofactors in the tetrachloroethene reductase of Dehalobacter restrictus. |
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| Journal |
FEBS. Lett. 409 (1997) 421-5. |
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| Reference |
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| Authors |
Schumacher W, Holliger C. |
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| Title |
The proton/electron ration of the menaquinone-dependent electron transport from dihydrogen to tetrachloroethene in "Dehalobacter restrictus". |
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| Journal |
J. Bacteriol. 178 (1996) 2328-33. |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: CAS: 163913-51-7 |
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| LinkDB |
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