Oxidoreductases;
Acting on diphenols and related substances as donors;
With oxygen as acceptor

3-amino-4-hydroxybenzoate:N-acetyl-L-cysteine:oxygen oxidoreductase

2 3-amino-4-hydroxybenzoate + N-acetyl-L-cysteine + 2 O2 = grixazone B + 4 H2O + CO2 [RN:R10720]

R10720

3-amino-4-hydroxybenzoate [CPD:C12115];
N-acetyl-L-cysteine [CPD:C06809];
O2 [CPD:C00007]

A type 3 multi copper protein. The enzyme, isolated from the bacterium Streptomyces griseus, catalyses an 8 electron oxidation. Activation of the enzyme requires a copper chaperone (GriE). It also acts on 3-amino-4-hydroxybenzaldehyde, giving grixazone A. The second aldehyde group is presumably lost as formate. The enzyme also catalyses the reaction of EC 1.10.3.4 o-aminophenol oxidase.

EC 1.10.3.15 created 2014

1  [PMID:17003031]

Suzuki H, Ohnishi Y, Furusho Y, Sakuda S, Horinouchi S.

Novel benzene ring biosynthesis from C(3) and C(4) primary metabolites by two enzymes.

J. Biol. Chem. 281 (2006) 36944-51.

[sgr:SGR_4246]

2  [PMID:19268377]

Le Roes-Hill M, Goodwin C, Burton S

Phenoxazinone synthase: what's in a name?

Trends. Biotechnol. 27 (2009) 248-58.

[sgr:SGR_4246]