| Entry |
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| Name |
versatile peroxidase;
VP;
hybrid peroxidase;
polyvalent peroxidase;
reactive-black-5:hydrogen-peroxide oxidoreductase
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| Class |
Oxidoreductases;
Acting on a peroxide as acceptor;
Peroxidases
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| Sysname |
1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol:hydrogen-peroxide oxidoreductase
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| Reaction(IUBMB) |
(1) 1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol + H2O2 = 4-hydroxy-3-methoxybenzaldehyde + 2-methoxyphenol + glycolaldehyde + H2O [RN: R11503];
(2) 2 manganese(II) + 2 H+ + H2O2 = 2 manganese(III) + 2 H2O [RN: R00011]
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| Reaction(KEGG) |
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| Substrate |
1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol [CPD: C21422];
H2O2 [CPD: C00027];
manganese [CPD: C00034];
H+ [CPD: C00080]
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| Product |
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| Comment |
A hemoprotein. This ligninolytic peroxidase combines the substrate-specificity characteristics of the two other ligninolytic peroxidases, EC 1.11.1.13, manganese peroxidase and EC 1.11.1.14, lignin peroxidase. Unlike these two enzymes, it is also able to oxidize phenols, hydroquinones and both low- and high-redox-potential dyes, due to a hybrid molecular architecture that involves multiple binding sites for substrates [2,4].
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| History |
EC 1.11.1.16 created 2006, modified 2016
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| Reference |
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| Authors |
Martinez MJ, Ruiz-Duenas FJ, Guillen F, Martinez AT. |
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| Title |
Purification and catalytic properties of two manganese peroxidase isoenzymes from Pleurotus eryngii. |
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| Journal |
Eur. J. Biochem. 237 (1996) 424-32. |
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| Reference |
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| Authors |
Heinfling A, Ruiz-Duenas FJ, Martinez MJ, Bergbauer M, Szewzyk U, Martinez AT. |
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| Title |
A study on reducing substrates of manganese-oxidizing peroxidases from Pleurotus eryngii and Bjerkandera adusta. |
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| Journal |
FEBS. Lett. 428 (1998) 141-6. |
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| Reference |
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| Authors |
Ruiz-Duenas FJ, Martinez MJ, Martinez AT. |
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| Title |
Molecular characterization of a novel peroxidase isolated from the ligninolytic fungus Pleurotus eryngii. |
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| Journal |
Mol. Microbiol. 31 (1999) 223-35. |
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| Reference |
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| Authors |
Camarero S, Sarkar S, Ruiz-Duenas FJ, Martinez MJ, Martinez AT. |
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| Title |
Description of a versatile peroxidase involved in the natural degradation of lignin that has both manganese peroxidase and lignin peroxidase substrate interaction sites. |
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| Journal |
J. Biol. Chem. 274 (1999) 10324-30. |
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| Sequence |
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| Reference |
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| Authors |
Ruiz-Duenas FJ, Martinez MJ, Martinez AT. |
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| Title |
Heterologous expression of Pleurotus eryngii peroxidase confirms its ability to oxidize Mn(2+) and different aromatic substrates. |
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| Journal |
Appl. Environ. Microbiol. 65 (1999) 4705-7. |
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| Reference |
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| Authors |
Camarero S, Ruiz-Duenas FJ, Sarkar S, Martinez MJ, Martinez AT. |
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| Title |
The cloning of a new peroxidase found in lignocellulose cultures of Pleurotus eryngii and sequence comparison with other fungal peroxidases. |
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| Journal |
FEMS. Microbiol. Lett. 191 (2000) 37-43. |
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| Sequence |
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| Reference |
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| Authors |
Ruiz-Duenas FJ, Camarero S, Perez-Boada M, Martinez MJ, Martinez AT. |
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| Title |
A new versatile peroxidase from Pleurotus. |
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| Journal |
Biochem. Soc. Trans. 29 (2001) 116-22. |
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| Reference |
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| Authors |
Banci L, Camarero S, Martinez AT, Martinez MJ, Perez-Boada M, Pierattelli R, Ruiz-Duenas FJ. |
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| Title |
NMR study of manganese(II) binding by a new versatile peroxidase from the white-rot fungus Pleurotus eryngii. |
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| Journal |
J. Biol. Inorg. Chem. 8 (2003) 751-60. |
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| Reference |
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| Authors |
Perez-Boada M, Ruiz-Duenas FJ, Pogni R, Basosi R, Choinowski T, Martinez MJ, Piontek K, Martinez AT. |
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| Title |
Versatile peroxidase oxidation of high redox potential aromatic compounds: site-directed mutagenesis, spectroscopic and crystallographic investigation of three long-range electron transfer pathways. |
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| Journal |
J. Mol. Biol. 354 (2005) 385-402. |
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| Reference |
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| Authors |
Caramelo L, Martinez MJ, Martinez AT. |
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| Title |
A search for ligninolytic peroxidases in the fungus pleurotus eryngii involving alpha-keto-gamma-thiomethylbutyric acid and lignin model dimers |
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| Journal |
Appl. Environ. Microbiol. 65 (1999) 916-22. |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: CAS: 42613-30-9 114995-15-2 |
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| LinkDB |
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