Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)

Renilla-luciferin:oxygen 2-oxidoreductase (decarboxylating)

Renilla luciferin + O2 = oxidized Renilla luciferin + CO2 + hnu [RN:R03137]

R03137

Renilla luciferin [CPD:C00982];
O2 [CPD:C00007]

From the soft coral coelenterate Renilla reniformis. The luciferin is bound to a luciferin-binding protein (BP-LH2). The bioluminescent reaction between the luciferin complex, luciferase and oxygen is triggered by calcium ions. In vivo, the excited state luciferin---luciferase complex undergoes the process of nonradiative energy transfer to an accessory protein, Renilla green fluorescent protein, which results in green bioluminescence. In vitro, Renilla luciferase emits blue light in the absence of any green fluorescent protein.

EC 1.13.12.5 created 1976, modified 1981, modified 1982, modified 2004

1  [PMID:4154104]

Cormier MJ, Hori K, Anderson JM.

Bioluminescence in coelenterates.

Biochim. Biophys. Acta. 346 (1974) 137-64.

2  [PMID:237531]

Hori K, Anderson JM, Ward WW, Cormier MJ.

Renilla luciferin as the substrate for calcium induced photoprotein bioluminescence. Assignment of luciferin tautomers in aequorin and mnemiopsin.

Biochemistry. 14 (1975) 2371-6.

3  [PMID:236561]

Shimomura O, Johnson FH.

Chemical nature of bioluminescence systems in coelenterates.

Proc. Natl. Acad. Sci. U. S. A. 72 (1975) 1546-9.

4  [PMID:33174]

Charbonneau H, Cormier MJ.

Ca2+-induced bioluminescence in Renilla reniformis. Purification and characterization of a calcium-triggered luciferin-binding protein.

J. Biol. Chem. 254 (1979) 769-80.

5  [PMID:4149963]

Anderson JM, Charbonneau H, Cormier MJ.

Mechanism of calcium induction of Renilla bioluminescence. Involvement of a calcium-triggered luciferin binding protein.

Biochemistry. 13 (1974) 1195-200.

6  [PMID:1674607]

Lorenz WW, McCann RO, Longiaru M, Cormier MJ.

Isolation and expression of a cDNA encoding Renilla reniformis luciferase.

Proc. Natl. Acad. Sci. U. S. A. 88 (1991) 4438-42.

[ag:AAA29804]