Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)

Photinus-luciferin:oxygen 4-oxidoreductase (decarboxylating, ATP-hydrolysing)

Photinus luciferin + O2 + ATP = oxidized Photinus luciferin + CO2 + AMP + diphosphate + hnu [RN:R04036]

R04036

Photinus luciferin [CPD:C02740];
O2 [CPD:C00007];
ATP [CPD:C00002]

Photinus (firefly) is a bioluminescent insect. The first step in the reaction is the formation of an acid anhydride between the carboxylic group and AMP, with the release of diphosphate. The enzyme may be assayed by measurement of light emission.

EC 1.13.12.7 created 1976, modified 1981, modified 1982

1  [PMID:6064360]

Hopkins TA, Seliger HH, White EH, Cass MH.

The chemiluminescence of firefly luciferin. A model for the bioluminescent reaction and identification of the product excited state.

J. Am. Chem. Soc. 89 (1967) 7148-50.

2

White, E.H., McCapra, F., Field, G.F. and McElroy, W.D.

The structure and synthesis of firefly luciferin.

J. Am. Chem. Soc. 83 (1961) 2402-2403.

3  [PMID:5784183]

White EH, Rapaport E, Hopkins TA, Seliger HH.

Chemi- and bioluminescence of firefly luciferin.

J. Am. Chem. Soc. 91 (1969) 2178-80.