| Entry |
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| Name |
deacetoxyvindoline 4-hydroxylase;
desacetoxyvindoline 4-hydroxylase;
desacetyoxyvindoline-17-hydroxylase;
D17H;
desacetoxyvindoline,2-oxoglutarate:oxygen oxidoreductase (4beta-hydroxylating)
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| Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
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| Sysname |
deacetoxyvindoline,2-oxoglutarate:oxygen oxidoreductase (4beta-hydroxylating)
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| Reaction(IUBMB) |
deacetoxyvindoline + 2-oxoglutarate + O2 = deacetylvindoline + succinate + CO2 [RN: R05857]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
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| Comment |
Requires Fe2+ and ascorbate. Also acts on 3-hydroxy-16-methoxy-2,3-dihydrotabersonine and to a lesser extent on 16-methoxy-2,3-dihydrotabersonine.
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| History |
EC 1.14.11.20 created 2002, modified 2005
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| Pathway |
| Indole alkaloid biosynthesis |
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| Orthology |
| deacetoxyvindoline 4-hydroxylase |
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| Reference |
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| Authors |
De Carolis E, Chan F, Balsevich J, De Luca V |
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| Title |
Isolation and Characterization of a 2-Oxoglutarate Dependent Dioxygenase Involved in the Second-to-Last Step in Vindoline Biosynthesis. |
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| Journal |
Plant. Physiol. 94 (1990) 1323-1329. |
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| Sequence |
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| Reference |
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| Authors |
De Carolis E, De Luca V. |
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| Title |
Purification, characterization, and kinetic analysis of a 2-oxoglutarate-dependent dioxygenase involved in vindoline biosynthesis from Catharanthus roseus. |
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| Journal |
J. Biol. Chem. 268 (1993) 5504-11. |
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| Sequence |
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| Reference |
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| Authors |
Vazquez-Flota FA, De Luca V. |
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| Title |
Developmental and light regulation of desacetoxyvindoline 4-hydroxylase in catharanthus roseus (L.) G. Don. . Evidence Of a multilevel regulatory mechanism |
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| Journal |
Plant. Physiol. 117 (1998) 1351-61. |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: CAS: 132084-83-4 |
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| LinkDB |
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