Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor

dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase

a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]

R07368 > R02160 R03126 R06539 R08082

dihydroflavonol [CPD:C15570];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]

In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from the satsuma Citrus unshiu also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.

EC 1.14.11.23 created 2004

1  [PMID:12180990]

Wellmann F, Lukacin R, Moriguchi T, Britsch L, Schiltz E, Matern U.

Functional expression and mutational analysis of flavonol synthase from Citrus unshiu.

Eur. J. Biochem. 269 (2002) 4134-42.

[ag:BAA36554]

2  [PMID:12620339]

Lukacin R, Wellmann F, Britsch L, Martens S, Matern U.

Flavonol synthase from Citrus unshiu is a bifunctional dioxygenase.

Phytochemistry. 62 (2003) 287-92.

3  [PMID:12782296]

Martens S, Forkmann G, Britsch L, Wellmann F, Matern U, Lukacin R.

Divergent evolution of flavonoid 2-oxoglutarate-dependent dioxygenases in parsley.

FEBS. Lett. 544 (2003) 93-8.

[ag:AAP57395]

4  [PMID:14570878]

Turnbull JJ, Nakajima J, Welford RW, Yamazaki M, Saito K, Schofield CJ.

Mechanistic studies on three 2-oxoglutarate-dependent oxygenases of flavonoid biosynthesis: anthocyanidin synthase, flavonol synthase, and flavanone 3beta-hydroxylase.

J. Biol. Chem. 279 (2004) 1206-16.