| Entry |
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| Name |
deacetoxycephalosporin-C hydroxylase;
deacetylcephalosporin C synthase;
3'-methylcephem hydroxylase;
DACS;
DAOC hydroxylase;
deacetoxycephalosporin C hydroxylase
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| Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
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| Sysname |
deacetoxycephalosporin-C,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating)
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| Reaction(IUBMB) |
deacetoxycephalosporin C + 2-oxoglutarate + O2 = deacetylcephalosporin C + succinate + CO2 [RN: R05229]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
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| Comment |
Requires iron(II). The enzyme can also use 3-exomethylenecephalosporin C as a substrate to form deacetoxycephalosporin C, although more slowly [2]. In Acremonium chrysogenum, the enzyme forms part of a bifunctional protein along with EC 1.14.20.1, deactoxycephalosporin-C synthase. It is a separate enzyme in Streptomyces clavuligerus.
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| History |
EC 1.14.11.26 created 2005
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| Pathway |
| Penicillin and cephalosporin biosynthesis | | Metabolic pathways | | Biosynthesis of antibiotics |
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| Orthology |
| deacetoxycephalosporin-C hydroxylase |
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| Genes |
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| Reference |
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| Authors |
Dotzlaf JE, Yeh WK. |
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| Title |
Copurification and characterization of deacetoxycephalosporin C synthetase/hydroxylase from Cephalosporium acremonium. |
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| Journal |
J. Bacteriol. 169 (1987) 1611-8. |
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| Reference |
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| Authors |
Baker BJ, Dotzlaf JE, Yeh WK. |
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| Title |
Deacetoxycephalosporin C hydroxylase of Streptomyces clavuligerus. Purification, characterization, bifunctionality, and evolutionary implication. |
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| Journal |
J. Biol. Chem. 266 (1991) 5087-93. |
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| Sequence |
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| Reference |
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| Authors |
Coque JJ, Enguita FJ, Cardoza RE, Martin JF, Liras P. |
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| Title |
Characterization of the cefF gene of Nocardia lactamdurans encoding a 3'-methylcephem hydroxylase different from the 7-cephem hydroxylase. |
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| Journal |
Appl. Microbiol. Biotechnol. 44 (1996) 605-9. |
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| Sequence |
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| Reference |
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| Authors |
Ghag SK, Brems DN, Hassell TC, Yeh WK. |
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| Title |
Refolding and purification of Cephalosporium acremonium deacetoxycephalosporin C synthetase/hydroxylase from granules of recombinant Escherichia coli. |
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| Journal |
Biotechnol. Appl. Biochem. 24 ( Pt 2) (1996) 109-19. |
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| Reference |
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| Authors |
Lloyd MD, Lipscomb SJ, Hewitson KS, Hensgens CM, Baldwin JE, Schofield CJ. |
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| Title |
Controlling the substrate selectivity of deacetoxycephalosporin/deacetylcephalosporin C synthase. |
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| Journal |
J. Biol. Chem. 279 (2004) 15420-6. |
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| Reference |
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| Authors |
Wu XB, Fan KQ, Wang QH, Yang KQ. |
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| Title |
C-terminus mutations of Acremonium chrysogenum deacetoxy/deacetylcephalosporin C synthase with improved activity toward penicillin analogs. |
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| Journal |
FEMS. Microbiol. Lett. 246 (2005) 103-10. |
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| Reference |
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| Authors |
Martin JF, Gutierrez S, Fernandez FJ, Velasco J, Fierro F, Marcos AT, Kosalkova K. |
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| Title |
Expression of genes and processing of enzymes for the biosynthesis of penicillins and cephalosporins. |
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| Journal |
Antonie. Van. Leeuwenhoek. 65 (1994) 227-43. |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: CAS: 69772-89-0 |
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| LinkDB |
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