Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor

L-arginine,2-oxoglutarate:oxygen oxidoreductase (succinate-forming)

2-oxoglutarate + L-arginine + O2 = succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O (overall reaction) [RN:R09785];
(1a) 2-oxoglutarate + L-arginine + O2 = succinate + CO2 + L-hydroxyarginine [RN:R10081];
(1b) L-hydroxyarginine = guanidine + (S)-1-pyrroline-5-carboxylate + H2O [RN:R10082]

R09785 R10081 R10082

2-oxoglutarate [CPD:C00026];
L-arginine [CPD:C00062];
O2 [CPD:C00007];
L-hydroxyarginine [CPD:C20330]

This is one of two simultaneous reactions catalysed by the enzyme, which is responsible for ethylene production in bacteria of the Pseudomonas syringae group.
In the other reaction [EC 1.13.12.19, 2-oxoglutarate dioxygenase (ethylene-forming)] the enzyme catalyses the dioxygenation of 2-oxoglutarate forming ethylene and three molecules of carbon dioxide.
The enzyme catalyses two cycles of the ethylene-forming reaction for each cycle of the succinate-forming reaction, so that the stoichiometry of the products ethylene and succinate is 2:1.

EC 1.14.11.34 created 2011

1  [PMID:1770346]

Nagahama K, Ogawa T, Fujii T, Tazaki M, Tanase S, Morino Y, Fukuda H

Purification and properties of an ethylene-forming enzyme from Pseudomonas syringae pv. phaseolicola PK2.

J. Gen. Microbiol. 137 (1991) 2281-6.

[ag:BAA02477]

2  [PMID:1445291]

Fukuda H, Ogawa T, Tazaki M, Nagahama K, Fujii T, Tanase S, Morino Y

Two reactions are simultaneously catalyzed by a single enzyme: the arginine-dependent simultaneous formation of two products, ethylene and succinate, from 2-oxoglutarate by an enzyme from Pseudomonas syringae.

Biochem. Biophys. Res. Commun. 188 (1992) 483-9.

[ag:BAA02477]

3  [PMID:1445325]

Fukuda H, Ogawa T, Ishihara K, Fujii T, Nagahama K, Omata T, Inoue Y, Tanase S, Morino Y

Molecular cloning in Escherichia coli, expression, and nucleotide sequence of the gene for the ethylene-forming enzyme of Pseudomonas syringae pv. phaseolicola PK2.

Biochem. Biophys. Res. Commun. 188 (1992) 826-32.

[ag:BAA02477]