| Entry |
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| Name |
kanamycin B dioxygenase;
kanJ (gene name)
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| Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
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| Sysname |
kanamycin-B,2-oxoglutarate:oxygen oxidoreductase (deaminating, 2'-hydroxylating)
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| Reaction(IUBMB) |
kanamycin B + 2-oxoglutarate + O2 = 2'-dehydrokanamycin A + succinate + NH3 + CO2 [RN: R10312]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
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| Comment |
Requires Fe2+ and ascorbate. Found in the bacterium Streptomyces kanamyceticus where it is involved in the conversion of the aminoglycoside antibiotic kanamycin B to kanamycin A.
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| History |
EC 1.14.11.37 created 2013, modified 2013
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| Pathway |
| Neomycin, kanamycin and gentamicin biosynthesis | | Biosynthesis of antibiotics |
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| Orthology |
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| Reference |
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| Authors |
Sucipto H, Kudo F, Eguchi T |
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| Title |
The last step of kanamycin biosynthesis: unique deamination reaction catalyzed by the alpha-ketoglutarate-dependent nonheme iron dioxygenase KanJ and the NADPH-dependent reductase KanK. |
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| Journal |
Angew. Chem. Int. Ed. Engl. 51 (2012) 3428-31. |
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| Sequence |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: |
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| LinkDB |
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