ENZYME: 1.14.11.51
Entry
EC 1.14.11.51 Enzyme
Name
DNA N6-methyladenine demethylase;
ALKBH1
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
Sysname
DNA-N6-methyladenosine,2-oxoglutarate:oxygen oxidoreductase (formaldehyde-forming)
Reaction(IUBMB)
N6-methyladenine in DNA + 2-oxoglutarate + O2 = adenine in DNA + formaldehyde + succinate + CO2 [RN:
R11345
]
Reaction(KEGG)
R11345
Substrate
N6-methyladenine in DNA [CPD:
C03391
];
2-oxoglutarate [CPD:
C00026
];
O2 [CPD:
C00007
]
Product
adenine in DNA [CPD:
C00821
];
formaldehyde [CPD:
C00067
];
succinate [CPD:
C00042
];
CO2 [CPD:
C00011
]
Comment
Contains iron(II). Catalyses oxidative demethylation of DNA N6-methyladenine, a prevalent modification in LINE-1 transposons, which are specifically enriched on the human X chromosome.
History
EC 1.14.11.51 created 2016
Reference
1 [PMID:
27027282
]
Authors
Wu TP, Wang T, Seetin MG, Lai Y, Zhu S, Lin K, Liu Y, Byrum SD, Mackintosh SG, Zhong M, Tackett A, Wang G, Hon LS, Fang G, Swenberg JA, Xiao AZ
Title
DNA methylation on N(6)-adenine in mammalian embryonic stem cells.
Journal
Nature. 532 (2016) 329-33.
Other DBs
ExplorEnz - The Enzyme Database:
1.14.11.51
IUBMB Enzyme Nomenclature:
1.14.11.51
ExPASy - ENZYME nomenclature database:
1.14.11.51
BRENDA, the Enzyme Database:
1.14.11.51
LinkDB
DBGET
integrated database retrieval system
