| Entry |
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| Name |
L-proline trans-4-hydroxylase
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| Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
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| Sysname |
L-proline,2-oxoglutarate:oxygen oxidoreductase (trans-4-hydroxylating)
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| Reaction(IUBMB) |
L-proline + 2-oxoglutarate + O2 = trans-4-hydroxy-L-proline + succinate + CO2 [RN: R01252]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
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| Comment |
Requires Fe2+ and ascorbate. The enzyme, isolated from multiple bacterial species, only produces trans-4-hydroxy-L-proline (cf. EC 1.14.11.56, L-proline cis-4-hydroxylase).
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| History |
EC 1.14.11.57 created 2017
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| Orthology |
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| Reference |
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| Authors |
Lawrence CC, Sobey WJ, Field RA, Baldwin JE, Schofield CJ |
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| Title |
Purification and initial characterization of proline 4-hydroxylase from Streptomyces griseoviridus P8648: a 2-oxoacid, ferrous-dependent dioxygenase involved in etamycin biosynthesis. |
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| Journal |
Biochem. J. 313 ( Pt 1) (1996) 185-91. |
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| Reference |
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| Authors |
Shibasaki T, Mori H, Chiba S, Ozaki A |
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| Title |
Microbial proline 4-hydroxylase screening and gene cloning. |
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| Journal |
Appl. Environ. Microbiol. 65 (1999) 4028-31. |
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| Sequence |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: |
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| LinkDB |
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