KEGG   ENZYME: 1.14.11.9Help
Entry
EC 1.14.11.9                Enzyme                                 

Name
flavanone 3-dioxygenase;
naringenin 3-hydroxylase;
flavanone 3-hydroxylase;
flavanone 3beta-hydroxylase;
flavanone synthase I;
(2S)-flavanone 3-hydroxylase;
naringenin,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating);
F3H;
flavanone,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
BRITE hierarchy
Sysname
(2S)-flavan-4-one,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating)
Reaction(IUBMB)
a (2S)-flavan-4-one + 2-oxoglutarate + O2 = a (2R,3R)-dihydroflavonol + succinate + CO2 [RN:R07329]
Reaction(KEGG)
Substrate
(2S)-flavan-4-one [CPD:C02099];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
(2R,3R)-dihydroflavonol [CPD:C21417];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
Requires Fe2+ and ascorbate. This plant enzyme catalyses an early step in the flavonoid biosynthesis pathway, leading to the production of flavanols and anthocyanins. Substrates include (2S)-naringenin, (2S)-eriodictyol, (2S)-dihydrotricetin and (2S)-pinocembrin. Some enzymes are bifuctional and also catalyse EC 1.14.11.23, flavonol synthase.
History
EC 1.14.11.9 created 1983, modified 1989, modified 2004, modified 2016
Pathway
Flavonoid biosynthesis
Metabolic pathways
Biosynthesis of secondary metabolites
Orthology
K00475  
naringenin 3-dioxygenase
Genes
ATH: 
ALY: 
CRB: 
EUS: 
BRP: 
BNA: 
THJ: 
CIT: 
CIC: 
TCC: 
GRA: 
105763220 105803713
GHI: 
107912810 107912992 107945804
EGR: 
104422756
GMX: 
100780057(f3h) 100799462 100815441(F3H) 100818312 732548(F3H)
PVU: 
PHAVU_003G261900g
VRA: 
106769061
VAR: 
108323664
MTR: 
MTR_3g058610 MTR_5g022020 MTR_8g075830 MTR_8g075890
CAM: 
101492596 101492925 101501974
ADU: 
107476683
AIP: 
107622822
LJA: 
Lj3g3v1487200.1(Lj3g3v1487200.1) Lj4g3v1037630.1(Lj4g3v1037630.1)
LANG: 
109330585 109332349 109359463
FVE: 
101300182
PPER: 
18770366
PMUM: 
103340206
MDM: 
103400025(F3H) 103436139(MdF3H)
PXB: 
103953484
ZJU: 
107424368
CSV: 
101206443 101215613
CMO: 
103491109 103498778
RCU: 
8268714
JCU: 
105629005
POP: 
POPTR_0005s11570g POPTR_0005s11600g POPTR_0005s11610g POPTR_1289s002001 POPTR_1289s002002
VVI: 
100233079(F3H) 100253950 104879126
SLY: 
100736482(F3H)
SPEN: 
107011910
SOT: 
102577463
INI: 
109167724(F3H) 109167725 109177773
SIND: 
105166042
BVG: 
104895196 104905115 104905116 104905117 104905155
NNU: 
104587834 104588723 104605233 104605566
OSA: 
9270463
DOSA: 
Os04t0662600-00(Os04g0662600)
OBR: 
102719670
BDI: 
100823527
ATS: 
F775_13583
ZMA: 
542712(fht1)
SITA: 
101777126
PDA: 
103697163 103708217
EGU: 
105054663 105059410
MUS: 
103970367 103992006
ATR: 
18441761 18441765
 » show all
Taxonomy
Reference
1
  Authors
Forkmann, G., Heller, W. and Grisebach, H.
  Title
Anthocyanin biosynthesis in flowers of Matthiola incana flavanone 3- and flavonoid 3'-hydroxylases.
  Journal
Z. Naturforsch. C: Biosci. 35 (1980) 691-695.
Reference
2  [PMID:8541503]
  Authors
Charrier B, Coronado C, Kondorosi A, Ratet P
  Title
Molecular characterization and expression of alfalfa (Medicago sativa L.) flavanone-3-hydroxylase and dihydroflavonol-4-reductase encoding genes.
  Journal
Plant. Mol. Biol. 29 (1995) 773-86.
Reference
3  [PMID:8685272]
  Authors
Pelletier MK, Shirley BW
  Title
Analysis of flavanone 3-hydroxylase in Arabidopsis seedlings. Coordinate regulation with chalcone synthase and chalcone isomerase.
  Journal
Plant. Physiol. 111 (1996) 339-45.
Reference
4  [PMID:15013767]
  Authors
Wellmann F, Matern U, Lukacin R.
  Title
Significance of C-terminal sequence elements for Petunia flavanone 3beta-hydroxylase activity.
  Journal
FEBS. Lett. 561 (2004) 149-54.
Reference
5  [PMID:16147863]
  Authors
Jin Z, Grotewold E, Qu W, Fu G, Zhao D
  Title
Cloning and characterization of a flavanone 3-hydroxylase gene from Saussurea medusa.
  Journal
DNA. Seq. 16 (2005) 121-9.
Reference
6  [PMID:16779664]
  Authors
Shen G, Pang Y, Wu W, Deng Z, Zhao L, Cao Y, Sun X, Tang K
  Title
Cloning and characterization of a flavanone 3-hydroxylase gene from Ginkgo biloba.
  Journal
Biosci. Rep. 26 (2006) 19-29.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
75991-43-4
LinkDB All DBs

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