| Entry |
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| Name |
3-methyl-2-oxobutanoate dehydrogenase (ferredoxin);
2-ketoisovalerate ferredoxin reductase;
3-methyl-2-oxobutanoate synthase (ferredoxin);
VOR;
branched-chain ketoacid ferredoxin reductase;
branched-chain oxo acid ferredoxin reductase;
keto-valine-ferredoxin oxidoreductase;
ketoisovalerate ferredoxin reductase;
2-oxoisovalerate ferredoxin reductase
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| Class |
Oxidoreductases;
Acting on the aldehyde or oxo group of donors;
With an iron-sulfur protein as acceptor
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| Sysname |
3-methyl-2-oxobutanoate:ferredoxin oxidoreductase (decarboxylating; CoA-2-methylpropanoylating)
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| Reaction(IUBMB) |
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + H+ [RN: R07160]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
S-(2-methylpropanoyl)-CoA;
CO2 [CPD: C00011];
reduced ferredoxin [CPD: C00138];
H+ [CPD: C00080]
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| Comment |
The enzyme is CoA-dependent and contains thiamine diphosphate and iron-sulfur clusters. Preferentially utilizes 2-oxo-acid derivatives of branched chain amino acids, e.g. 3-methyl-2-oxopentanoate, 4-methyl-2-oxo-pentanoate, 2-oxobutyrate and 3-methylthiopropanamine. This enzyme is a member of the 2-oxoacid oxidoreductases, a family of enzymes that oxidatively decarboxylate different 2-oxoacids to form their CoA derivatives, and are differentiated based on their substrate specificity. For examples of other members of this family, see EC 1.2.7.1, pyruvate synthase, and EC 1.2.7.3, 2-oxoglutarate synthase.
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| History |
EC 1.2.7.7 created 2003
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| Pathway |
| Valine, leucine and isoleucine degradation | | Metabolic pathways |
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| Orthology |
| 2-oxoisovalerate ferredoxin oxidoreductase, alpha subunit | | 2-oxoisovalerate ferredoxin oxidoreductase, beta subunit | | 2-oxoisovalerate ferredoxin oxidoreductase, delta subunit | | 2-oxoisovalerate ferredoxin oxidoreductase, gamma subunit |
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| Genes |
PEF: | | DEJ: | | DML: | | AFW: | | DAV: | | PBU: | | PBAS: | | SLR: | | ABA: | | SUS: | | CABY: | | MMP: | | MMQ: | | MMX: | | MMZ: | | MMD: | | MMAK: | | MMAO: | | MVN: | | MVO: | | MAC: | | MBA: | | MBY: | | MBW: | | MBAR: | | MBAK: | | MMA: | | MMAZ: | | MMJ: | | MMAC: | | MVC: | | MEK: | | MLS: | | METM: | | MEF: | | MEQ: | | MSJ: | | MSZ: | | MSW: | | MTHE: | | MTHR: | | MHOR: | | MBU: | | MMET: | | MMH: | | MHAZ: | | MZH: | | MPY: | | MHZ: | | MTP: | | MCJ: | | MHI: | | MTH: | | MMG: | | METC: | | MWO: | | MSI: | | MRU: | | MMIL: | | MEYE: | | MOL: | | MEL: | | MEW: | | METH: | | MFC: | | MFI: | | MCUB: | | MFV: | | TAC: | | TVO: | | MER: | | MEAR: | | PHO: | | PAB: | | PFU: | | PFI: | | PYN: | | PYA: | | PYS: | | PYC: | | TKO: | | TON: | | TGA: | | TSI: | | TBA: | | THE: | | THA: | | THM: | | TLT: | | THS: | | TNU: | | TEU: | | TGY: | | THV: | | TCH: | | TPEP: | | PPAC: | | ABI: | | ACF: | | KCR: | |
» show all
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| Reference |
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| Authors |
Heider J, Mai X, Adams MW. |
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| Title |
Characterization of 2-ketoisovalerate ferredoxin oxidoreductase, a new and reversible coenzyme A-dependent enzyme involved in peptide fermentation by hyperthermophilic archaea. |
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| Journal |
J. Bacteriol. 178 (1996) 780-7. |
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| Reference |
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| Authors |
Tersteegen A, Linder D, Thauer RK, Hedderich R. |
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| Title |
Structures and functions of four anabolic 2-oxoacid oxidoreductases in Methanobacterium thermoautotrophicum. |
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| Journal |
Eur. J. Biochem. 244 (1997) 862-8. |
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| Reference |
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| Authors |
Schut GJ, Menon AL, Adams MW. |
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| Title |
2-keto acid oxidoreductases from Pyrococcus furiosus and Thermococcus litoralis. |
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| Journal |
Methods. Enzymol. 331 (2001) 144-58. |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: |
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| LinkDB |
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