| Entry |
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| Name |
nitroalkane oxidase;
nitroethane oxidase;
NAO;
nitroethane:oxygen oxidoreductase
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| Class |
Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With oxygen as acceptor
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| Sysname |
nitroalkane:oxygen oxidoreductase
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| Reaction(IUBMB) |
a nitroalkane + H2O + O2 = an aldehyde or ketone + nitrite + H2O2 [RN: R10388 R10389]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
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| Comment |
Has an absolute requirement for FAD [4]. While nitroethane may be the physiological substrate [2], the enzyme also acts on several other nitroalkanes, including 1-nitropropane, 2-nitropropane, 1-nitrobutane, 1-nitropentane, 1-nitrohexane, nitrocyclohexane and some nitroalkanols [4]. Differs from EC 1.13.11.16, nitronate monooxygenase, in that the preferred substrates are neutral nitroalkanes rather than anionic nitronates [4].
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| History |
EC 1.7.3.1 created 1961, modified 2006, modified 2009
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| Pathway |
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| Orthology |
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| Genes |
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| Reference |
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| Authors |
LITTLE HN. |
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| Title |
Oxidation of nitroethane by extracts from Neurospora. |
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| Journal |
J. Biol. Chem. 193 (1951) 347-58. |
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| Reference |
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| Authors |
Kido T, Hashizume K, Soda K. |
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| Title |
Purification and properties of nitroalkane oxidase from Fusarium oxysporum. |
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| Journal |
J. Bacteriol. 133 (1978) 53-8. |
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| Reference |
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| Authors |
Daubner SC, Gadda G, Valley MP, Fitzpatrick PF. |
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| Title |
Cloning of nitroalkane oxidase from Fusarium oxysporum identifies a new member of the acyl-CoA dehydrogenase superfamily. |
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| Journal |
Proc. Natl. Acad. Sci. U. S. A. 99 (2002) 2702-7. |
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| Sequence |
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| Reference |
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| Authors |
Fitzpatrick PF, Orville AM, Nagpal A, Valley MP. |
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| Title |
Nitroalkane oxidase, a carbanion-forming flavoprotein homologous to acyl-CoA dehydrogenase. |
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| Journal |
Arch. Biochem. Biophys. 433 (2005) 157-65. |
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| Reference |
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| Authors |
Valley MP, Tichy SE, Fitzpatrick PF. |
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| Title |
Establishing the kinetic competency of the cationic imine intermediate in nitroalkane oxidase. |
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| Journal |
J. Am. Chem. Soc. 127 (2005) 2062-6. |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: UM-BBD (Biocatalysis/Biodegradation Database): BRENDA, the Enzyme Database: CAS: 9029-36-1 65802-82-6 |
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| LinkDB |
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