| Entry |
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|---|
| Name |
methylenetetrahydrofolate---tRNA-(uracil54-C5)-methyltransferase (FADH2-oxidizing);
folate-dependent ribothymidyl synthase;
methylenetetrahydrofolate-transfer ribonucleate uracil 5-methyltransferase;
5,10-methylenetetrahydrofolate:tRNA-UPsiC (uracil-5-)-methyl-transferase;
5,10-methylenetetrahydrofolate:tRNA (uracil-5-)-methyl-transferase;
TrmFO;
folate/FAD-dependent tRNA T54 methyltransferase
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| Class |
Transferases;
Transferring one-carbon groups;
Methyltransferases
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| Sysname |
5,10-methylenetetrahydrofolate:tRNA (uracil54-C5)-methyltransferase
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| Reaction(IUBMB) |
5,10-methylenetetrahydrofolate + uracil54 in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil54 in tRNA + FAD [RN: R03704]
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| Reaction(KEGG) |
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| Substrate |
5,10-methylenetetrahydrofolate [CPD: C00143];
uracil54 in tRNA;
FADH2 [CPD: C01352]
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| Product |
tetrahydrofolate [CPD: C00101];
5-methyluracil54 in tRNA;
FAD [CPD: C00016]
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| Comment |
Up to 25% of the bases in mature tRNA are post-translationally modified or hypermodified. One almost universal post-translational modification is the conversion of U54 into ribothymidine in the TPsiC loop, and this modification is found in most species studied to date [2]. Unlike this enzyme, which uses 5,10-methylenetetrahydrofolate and FADH2 to supply the atoms for methylation of U54, EC 2.1.1.35, tRNA (uracil54-C5)-methyltransferase, uses S-adenosyl-L-methionine.
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| History |
EC 2.1.1.74 created 1983 as EC 2.1.2.12, transferred 1984 to EC 2.1.1.74, modified 2011
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| Orthology |
| methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase |
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| Genes |
SNJ: | | GSU: | | GSK: | | GME: | | GUR: | | GLO: | | GBM: | | GEO: | | GEM: | | GEB: | | GPI: | | GAO: | | GSB: | | PCA: | | PPD: | | PACE: | | PEF: | | DES: | | DEU: | | DVU: | | DVL: | | DVM: | | DVG: | | DDE: | | DDS: | | DDN: | | DMA: | | DSA: | | DAS: | | DAF: | | DHY: | | DPI: | | DGG: | | DFI: | | DEJ: | | DPG: | | LIP: | | LIR: | | DBA: | | DOA: | | DRT: | | BBA: | | BBAT: | | BBW: | | BBAC: | | BEX: | | BMX: | | DPS: | | DAK: | | DPR: | | DSF: | | ADE: | | ACP: | | AFW: | | ANK: | | MXA: | | MFU: | | MSD: | | MYM: | | CCX: | | SUR: | | AGE: | | VIN: | | SCL: | | SCU: | | CCRO: | | SAMY: | | LLU: | | MRM: | | HOH: | | SAT: | | DAO: | | DTI: | | DBR: | | HMR: | | DAV: | | EAA: | | MLO: | | MLN: | | MCI: | | MOP: | | MAM: | | MAMO: | | MESO: | | MESW: | | MES: | | HOE: | | AAK: | | PLA: | | SME: | | SMK: | | SMQ: | | SMX: | | SMI: | | SMEG: | | SMEL: | | SMER: | | SMD: | | RHI: | | SFH: | | SFD: | | SIX: | | SAME: | | EAD: | | EAH: | | ATU: | | ARA: | | ATF: | | ATA: | | AVI: | | AGR: | | AGC: | | ARO: | | RET: | | REC: | | REL: | | REI: | | REP: | | RLE: | | RLT: | | RLG: | | RLB: | | RLU: | | RTR: | | RIR: | | RHL: | | RGA: | | RHN: | | RPHA: | | RHT: | | RHX: | | NGL: | | NGG: | | SHZ: | | BME: | | BMEL: | | BMI: | | BMZ: | | BMG: | | BMW: | | BMEE: | | BMF: | | BMB: | | BMC: | | BAA: | | BABO: | | BABR: | | BABT: | | BABB: | | BABU: | | BABS: | | BABC: | | BMS: | | BSI: | | BSF: | | BSUI: | | BSUP: | | BSUV: | | BSUC: | | BMT: | | BSZ: | | BSV: | | BSW: | | BSG: | | BOV: | | BCS: | | BSK: | | BOL: | | BCAR: | | BCAS: | | BMR: | | BPP: | | BPV: | | BCET: | | BCEE: | | BVL: | | OAN: | | OAH: | | OPS: | | BJA: | | BJU: | | BJP: | | BRA: | | BBT: | | BRS: | | AOL: | | BRC: | | BRAD: | | BIC: | | RPA: | | RPB: | | RPC: | | RPD: | | RPE: | | RPT: | | RPX: | | NWI: | | NHA: | | OCA: | | OCG: | | OCO: | | BOP: | | BOS: | | BVV: | | BHE: | | BHN: | | BHS: | | BQR: | | BBK: | | BTR: | | BTX: | | BGR: | | BCD: | | BAUS: | | BVN: | | BANC: | | XAU: | | AZC: | | SNO: | | MEX: | | MEA: | | MDI: | | MCH: | | MRD: | | MET: | | MPO: | | MNO: | | MOR: | | META: | | MAQU: | | BID: | | MSL: | | CHEL: | | CDQ: | | HDN: | | HDT: | | HMC: | | HNI: | | RVA: | | PHL: | | FIL: | | FIY: | | DEQ: | | BVR: | | RHZ: | | MEY: | | MAAD: | | MMED: | | AUA: | | MCG: | | THD: | | PSIN: | | RBM: | | CCR: | | CCS: | | CAK: | | CSE: | | CHQ: | | PZU: | | BSB: | | BRD: | | BNE: | | BRG: | | BRL: | | AEX: | | CBOT: | | SIL: | | SIT: | | RMB: | | RSP: | | RSH: | | RSQ: | | RSK: | | RCP: | | RHP: | | JAN: | | RDE: | | RLI: | | PDE: | | PAMI: | | PYE: | | PCON: | | DSH: | | KVU: | | KVL: | | KRO: | | PSF: | | PGA: | | PGL: | | PGD: | | PHP: | | OAT: | | OAR: | | OTM: | | LMD: | | RED: | | PTP: | | CID: | | CMAR: | | MALG: | | CON: | | RSU: | | RHM: | | RHC: | | PPHR: | | HAT: | | LAP: | | LAGG: | | DAA: | | YAN: | | SUAM: | | DON: | | TPRO: | | TOM: | | PABY: | | THW: | | RMM: | | LVS: | | MMR: | | HNE: | | HBA: | | HBC: | | ZMO: | | ZMN: | | ZMM: | | ZMB: | | ZMI: | | ZMC: | | ZMR: | | ZMP: | | NAR: | | NPP: | | NPN: | | NRE: | | SAL: | | SPHK: | | SPHP: | | SMAG: | | SMAZ: | | STER: | | SGI: | | SPHL: | | SPHQ: | | SWI: | | SPHM: | | STAX: | | SPHI: | | SSAN: | | SMY: | | SPAN: | | SKR: | | SPLM: | | SJP: | | SCH: | | SSY: | | SYB: | | SBD: | | SPMI: | | SPHB: | | SPHR: | | SINB: | | CIJ: | | SPHG: | | BLAS: | | ELI: | | ELQ: | | ERY: | | EGN: | | AAY: | | AMX: | | AEP: | | ANH: | | ADO: | | CNA: | | CMAN: | | PNS: | | PORL: | | RRU: | | RRF: | | RCE: | | RPM: | | MAG: | | MGY: | | MAGX: | | MAGN: | | AZL: | | ALI: | | ABS: | | ABQ: | | ABF: | | ATI: | | AHU: | | TMO: | | TXI: | | MAGQ: | | HJO: | | PBR: | | MGM: | | MAI: | | MAN: | | PGV: | | APB: | | BSU: | | BSR: | | BSL: | | BSH: | | BSY: | | BSUT: | | BSUL: | | BSUS: | | BSS: | | BST: | | BSO: | | BSN: | | BSQ: | | BSP: | | BLI: | | BLD: | | BLH: | | BAY: | | BAQ: | | BYA: | | BAMP: | | BAML: | | BAMA: | | BAMN: | | BAMB: | | BAMT: | | BAMY: | | BMP: | | BAO: | | BAZ: | | BQL: | | BXH: | | BQY: | | BAMI: | | BAMC: | | BAMF: | | BAE: | | BATR: | | BHA: | | BAN: | | BAR: | | BAT: | | BAH: | | BAI: | | BAX: | | BANT: | | BANR: | | BANS: | | BANH: | | BANV: | | BCE: | | BCA: | | BCZ: | | BCR: | | BCB: | | BCU: | | BCG: | | BCQ: | | BCX: | | BAL: | | BNC: | | BCF: | | BCER: | | BCEF: | | BCY: | | BTK: | | BTL: | | BTB: | | BTT: | | BTHR: | | BTHI: | | BTC: | | BTF: | | BTM: | | BTG: | | BTI: | | BTN: | | BTHT: | | BTHU: | | BTW: | | BTHY: | | BWE: | | BWW: | | BTY: | | BMYC: | | BMYO: | | BCL: | | BPU: | | BPUM: | | BPUS: | | BPF: | | BMQ: | | BMD: | | BMH: | | BMEG: | | BCO: | | BJS: | | BACI: | | BIF: | | BLE: | | BMET: | | GST: | | BACW: | | BACP: | | BACB: | | BBY: | | BACO: | | BACY: | | BACL: | | BALM: | | BEO: | | BSM: | | BSJ: | | BON: | | BGY: | | BFX: | | BGI: | | BWH: | | BXI: | | BHK: | | GWC: | | GMC: | | GTH: | | PTL: | | AFL: | | AGN: | | ANM: | | AAMY: | | ANL: | | LSP: | | LGY: | | LFU: | | FPN: | | FAR: | | SAU: | | SAV: | | SAW: | | SAH: | | SAJ: | | SAM: | | SAS: | | SAR: | | SAC: | | SAX: | | SAA: | | SAO: | | SAE: | | SAD: | | SUU: | | SUV: | | SUE: | | SUJ: | | SUK: | | SUC: | | SUT: | | SUQ: | | SUZ: | | SUD: | | SUX: | | SUW: | | SUG: | | SUF: | | SAUA: | | SAUE: | | SAUN: | | SAUS: | | SAUU: | | SAUG: | | SAUZ: | | SAUT: | | SAUJ: | | SAUK: | | SAUQ: | | SAUV: | | SAUW: | | SAUX: | | SAUY: | | SAB: | | SUY: | | SAUB: | | SAUM: | | SAUC: | | SAUR: | | SAUI: | | SAUD: | | SAUF: | | SAMS: | | SUH: | | SEP: | | SER: | | SEPP: | | SEPS: | | SHA: | | SHH: | | SSP: | | SCA: | | SLG: | | SLN: | | SSD: | | SDT: | | SWA: | | SPAS: | | SXY: | | SXL: | | SXO: | | SHU: | | SCAP: | | SSCH: | | SSCZ: | | SAGQ: | | SEQO: | | SSIF: | | MCL: | | MCAK: | | SBAC: | | LMO: | | LMF: | | LMH: | | LMC: | | LMN: | | LMY: | | LMT: | | LMG: | | LMS: | | LMJ: | | LMQ: | | LML: | | LMP: | | LMW: | | LMX: | | LMZ: | | LMON: | | LMOC: | | LMOS: | | LMOO: | | LMOY: | | LMOT: | | LMOA: | | LMOL: | | LMOG: | | LMOE: | | LMOB: | | LMOJ: | | LMOZ: | | LMOD: | | LMOW: | | LMOX: | | LMOQ: | | LMR: | | LMOK: | | LMV: | | LMOM: | | LIN: | | LWE: | | LSG: | | LIV: | | LII: | | LIW: | | LIA: | | LIO: | | ESI: | | EAT: | | EAN: | | EXM: | | EXU: | | GOT: | | BBE: | | BLR: | | PJD: | | GYM: | | PPY: | | PPM: | | PPO: | | PPOL: | | PPQ: | | PPOY: | | PMS: | | PMQ: | | PMW: | | PTA: | | PLV: | | PSAB: | | PDU: | | PGM: | | POD: | | PAEF: | | PSTE: | | PAEA: | | PAEE: | | PAEH: | | PAEJ: | | PBJ: | | PIH: | | PRI: | | PPEO: | | PNP: | | POW: | | PBV: | | PXL: | | PYG: | | PSWU: | | TCO: | | ANX: | | AAC: | | AAD: | | BTS: | | TUM: | | SIV: | | SSIL: | | PLN: | | PKU: | | PRT: | | PLL: | | PANA: | | PDG: | | PHC: | | PMAR: | | PPLA: | | JEO: | | KUR: | | SPSY: | | SPOR: | | SPOP: | | SURE: | | RST: | | LLA: | | LLK: | | LLT: | | LLS: | | LLD: | | LLX: | | LLC: | | LLM: | | LLR: | | LLN: | | LLI: | | LLW: | | LLJ: | | LGR: | | LGV: | | LPK: | | SPY: | | SPZ: | | SPYM: | | SPM: | | SPG: | | SPS: | | SPH: | | SPI: | | SPJ: | | SPK: | | SPF: | | SPA: | | SPB: | | STG: | | SOZ: | | STZ: | | STX: | | SPYA: | | SPYH: | | SPYO: | | SPN: | | SPD: | | SPR: | | SPW: | | SPX: | | SNE: | | SPV: | | SNM: | | SJJ: | | SPP: | | SNT: | | SNC: | | SNB: | | SNP: | | SNI: | | SNV: | | SNX: | | SND: | | SNU: | | SPNG: | | SPNE: | | SPNU: | | SPNM: | | SPNO: | | SPNN: | | SAG: | | SAN: | | SAK: | | SGC: | | SAGS: | | SAGL: | | SAGM: | | SAGI: | | SAGR: | | SAGP: | | SAGC: | | SAGT: | | SAGE: | | SAGG: | | SAGN: | | SMU: | | SMC: | | SMJ: | | SMUT: | | SMUA: | | STC: | | STL: | | STE: | | STN: | | STU: | | STW: | | STHE: | | STHS: | | SSA: | | SSB: | | SSU: | | SSV: | | SSI: | | SSS: | | SST: | | SSF: | | SSK: | | SSQ: | | SSW: | | SUI: | | SUO: | | SRP: | | SUP: | | SSUS: | | SSUT: | | SSUI: | | SSUY: | | SGO: | | SEQ: | | SEZO: | | SEQU: | | SEU: | | SUB: | | SDS: | | SDG: | | SDA: | | SDC: | | SDQ: | | SGA: | | SGG: | | SGT: | | SMB: | | SOR: | | STK: | | STB: | | SCP: | | SCF: | | SSR: | | STF: | | STJ: | | STRS: | | SSAH: | | STD: | | SMN: | | SIF: | | SIE: | | SIB: | | SIU: | | SANG: | | SANC: | | SANS: | | SCG: | | SCON: | | SCOS: | | SOI: | | SIK: | | SIQ: | | SIO: | | SIZ: | | SLU: | | SIG: | | SIP: | | STV: | | LPL: | | LPJ: | | LPT: | | LPS: | | LPR: | | LPZ: | | LPB: | | LJO: | | LJF: | | LJH: | | LJN: | | LAC: | | LAI: | | LAD: | | LAF: | | LSA: | | LSL: | | LSI: | | LSJ: | | LDB: | | LBU: | | LDE: | | LDL: | | LCA: | | LPQ: | | LPI: | | LPAP: | | LCB: | | LCZ: | | LCS: | | LCE: | | LCW: | | LCL: | | LCX: | | LGA: | | LHE: | | LHL: | | LHR: | | LHV: | | LHH: | | LHD: | | LRH: | | LRG: | | LRL: | | LRA: | | LRO: | | LRC: | | LCR: | | LAM: | | LAY: | | LKE: | | LRM: | | LAW: | | LAE: | | LGL: | | LPX: | | LJE: | | LBT: | | LCV: | | EFA: | | EFL: | | EFI: | | EFD: | | EFS: | | EFN: | | EFQ: | | EFC: | | EFAU: | | EFU: | | EFM: | | EFT: | | EHR: | | ECAS: | | EMU: | | EDU: | | EGA: | | ESS: | | MPS: | | MPX: | | VTE: | | VPI: | | CML: | | CLB: | | CTH: | | CTX: | | RUK: | | CCE: | | CCL: | | ESR: | | ESU: | | CSS: | | CSD: | | CCEL: | | EHA: | | RAL: | | RBR: | | RCH: | | RUS: | | RBP: | | FPR: | | FPA: | | CLO: | | STH: | | SWO: | | SLP: | | DSY: | | DHD: | | DDH: | | DDL: | | DMT: | | DRM: | | DAE: | | DCA: | | DKU: | | DRU: | | DGI: | | DFG: | | PTH: | | DAU: | | TJR: | | SGY: | | DOR: | | DAI: | | DMI: | | DED: | | DEC: | | DRS: | | HMO: | | OVA: | | TMR: | | SAY: | | SAP: | | IBU: | | FPLA: | | CHY: | | TEP: | | TAE: | | MTA: | | ADG: | | TPZ: | | CSC: | | ATE: | | COB: | | CHD: | | COW: | | CKI: | | CKN: | | CLC: | | TOC: | | CPO: | | TNR: | | MAS: | | NTH: | | HOR: | | VPR: | | VAT: | | MED: | | DPN: | | SSG: | | SRI: | | SELE: | | SELO: | | SELT: | | PUF: | | PFT: | | AFN: | | AIN: | | ERH: | | ERS: | | ERL: | | TUR: | | FRO: | | ERB: | | MMY: | | MMYM: | | MMYI: | | MML: | | MCP: | | MCAC: | | MCAP: | | MCAR: | | MCAI: | | MLC: | | MLH: | | MAA: | | MAL: | | MBV: | | MBH: | | MBI: | | MBQ: | | MPF: | | MPUT: | | MYT: | | ACL: | | ABRA: | | APAL: | | AOC: | | MFL: | | MFW: | | SCR: | | SSYR: | | SDI: | | STAI: | | SAPI: | | SMIA: | | SCQ: | | SSAB: | | SERI: | | STUR: | | SLL: | | SKN: | | SCJ: | | SHJ: | | MBJ: | | RXY: | | RRD: | | CCU: | | SHI: | | ELE: | | EYY: | | AEQ: | | DDT: | | APV: | | OLS: | | OLO: | | CGO: | | CBAC: | | SYN: | | SYZ: | | SYY: | | SYT: | | SYS: | | SYQ: | | SYJ: | | SYW: | | SYC: | | SYF: | | SYG: | | SYR: | | SYX: | | SYP: | | CYA: | | CYB: | | SYNE: | | SYNK: | | SYNR: | | SYND: | | SYU: | | SYH: | | SYNW: | | TEL: | | THN: | | CGC: | | CYI: | | CMP: | | LEP: | | LEN: | | LBO: | | PMN: | | PMF: | | PMH: | | PMJ: | | PRC: | | PRM: | | AMR: | | MAR: | | MPK: | | CAN: | | CSN: | | GLP: | | GEN: | | GEE: | | CYT: | | CYP: | | CYC: | | CYN: | | CYH: | | CYJ: | | MIC: | | GEI: | | OAC: | | MPRO: | | CEP: | | GVI: | | GLJ: | | ANA: | | NPU: | | NOS: | | NOP: | | NON: | | AVA: | | ANB: | | ACY: | | AWA: | | NAZ: | | CSG: | | CALO: | | CALT: | | CALH: | | RIV: | | FIS: | | NCN: | | CTHE: | | PLP: | | SCS: | | MBF: | | ATM: | | ABAO: | | ABAT: | | CAP: | | DRA: | | DGE: | | DDR: | | DMR: | | DPT: | | DGO: | | DPD: | | DSW: | | DCH: | | DAB: | | DPU: | | TRA: | | TTH: | | TTJ: | | TTS: | | TTL: | | TSC: | | THC: | | TOS: | | TAQ: | | TBC: | | MRB: | | MRE: | | MSV: | | OPR: | | MHD: | | CCZ: | | FGI: | | MIN: | | ABA: | | ACA: | | ACM: | | GMA: | | TSA: | | TRS: | | SUS: | | CTM: | | ABAC: | | FNU: | | FNC: | | FNT: | | FUS: | | FNE: | | FHW: | | IPO: | | TAI: | | ACO: | | TLI: | | AMO: | | CPOR: | | FSU: | | FSC: | | GAU: | | GPH: | | GBA: | | AAE: | | HYA: | | HHO: | | HYS: | | HTH: | | HTE: | | TAL: | | TRD: | | SUL: | | SAF: | | PMX: | | TTK: | | TAM: | | DTE: | | TMA: | | TMM: | | TMI: | | TMW: | | TMQ: | | TMX: | | TPT: | | TRQ: | | TNA: | | TNP: | | THQ: | | THZ: | | THR: | | TLE: | | TTA: | | PHY: | | TME: | | TAF: | | THP: | | THER: | | FNO: | | FPE: | | FIA: | | KOL: | | KPF: | | MPG: | | CEX: | | DIN: | | DDF: | | DAP: | | CNI: | | FSI: | | DTH: | | DTU: | | NDE: | | NMV: | | NIO: | | LFC: | | LFI: | | LFP: | | LEG: | | TID: | | TOP: | | TCM: | | CTHI: | | MOX: | |
» show all
 |
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| Reference |
|
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| Authors |
Delk AS, Nagle DP Jr, Rabinowitz JC. |
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| Title |
Methylenetetrahydrofolate-dependent biosynthesis of ribothymidine in transfer RNA of Streptococcus faecalis. Evidence for reduction of the 1-carbon unit by FADH2. |
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| Journal |
J. Biol. Chem. 255 (1980) 4387-90. |
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| Reference |
|
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| Authors |
Becker HF, Motorin Y, Sissler M, Florentz C, Grosjean H. |
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| Title |
Major identity determinants for enzymatic formation of ribothymidine and pseudouridine in the T psi-loop of yeast tRNAs. |
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| Journal |
J. Mol. Biol. 274 (1997) 505-18. |
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| Reference |
|
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| Authors |
Nishimasu H, Ishitani R, Yamashita K, Iwashita C, Hirata A, Hori H, Nureki O |
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| Title |
Atomic structure of a folate/FAD-dependent tRNA T54 methyltransferase. |
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| Journal |
Proc. Natl. Acad. Sci. U. S. A. 106 (2009) 8180-5. |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: CAS: 56831-74-4 |
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| LinkDB |
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