| Entry |
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| Name |
N-substituted formamide deformylase;
NfdA
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| Class |
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
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| Sysname |
N-benzylformamide amidohydrolase
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| Reaction(IUBMB) |
N-benzylformamide + H2O = formate + benzylamine [RN: R07303]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
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| Comment |
Zinc is a cofactor. While N-benzylformamide is the best substrate, the enzyme from Arthrobacter pascens can also act on the N-substituted formamides N-butylformamide, N-allylformamide, N-[2-(cyclohex-1-enyl)ethyl]formamide and N-(1-phenylethyl)formamide, but much more slowly. Amides of other acids do not act as substrates.
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| History |
EC 3.5.1.91 created 2005
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| Orthology |
| N-substituted formamide deformylase |
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| Reference |
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| Authors |
Fukatsu H, Hashimoto Y, Goda M, Higashibata H, Kobayashi M. |
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| Title |
Amine-synthesizing enzyme N-substituted formamide deformylase: screening, purification, characterization, and gene cloning. |
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| Journal |
Proc. Natl. Acad. Sci. U. S. A. 101 (2004) 13726-31. |
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| Sequence |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: CAS: 115299-95-1 |
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| LinkDB |
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