KEGG ENZYME: 3.5.5.1

ENZYME: 3.5.5.1

Entry

EC 3.5.5.1 Enzyme

Name

nitrilase;
acetonitrilase;
benzonitrilase

Class

Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In nitriles

Sysname

nitrile aminohydrolase

Reaction(IUBMB)

a nitrile + 2 H2O = a carboxylate + NH3 [RN:R00540]

Reaction(KEGG)

R00540 > R01887 R03093 R03542 R05591 R07855

Substrate

nitrile [CPD:C00726];
H2O [CPD:C00001]

Product

carboxylate [CPD:C00060];
NH3 [CPD:C00014]

Comment

Acts on a wide range of aromatic nitriles including (indol-3-yl)acetonitrile, and also on some aliphatic nitriles, and on the corresponding acid amides. cf. EC 4.2.1.84 nitrile hydratase.

History

EC 3.5.5.1 created 1965, modified 1989

Pathway

ec00380	Tryptophan metabolism
ec00460	Cyanoamino acid metabolism
ec00627	Aminobenzoate degradation
ec00643	Styrene degradation
ec00910	Nitrogen metabolism
ec01120	Microbial metabolism in diverse environments

Orthology

K01501

nitrilase

Genes

CEL:	CELE_ZK1058.6(nit-1)
CBR:	CBG03597(Cbr-nit-1)
ATH:	AT3G44300(NIT2) AT3G44310(NIT1) AT3G44320(NIT3)
ALY:	ARALYDRAFT_323132 ARALYDRAFT_484849
CRB:	CARUB_v10005182mg CARUB_v10017504mg CARUB_v10017572mg
EUS:	EUTSA_v10010499mg EUTSA_v10010504mg EUTSA_v10023557mg
BRP:	103848073 103851084(NIT-T1) 103856931(BrNIT3) 103865089(BrNIT1)
BNA:	106348366 106402388
RCU:	8268610
OSA:	4330076(OJ1626_B09.5)
DOSA:	Os02t0635000-01(Os02g0635000)
BDI:	100822233
SBI:	8074792
ZMA:	542573(NIT1)
SITA:	101766965
LTH:	KLTH0E00330g
PPA:	PAS_chr2-1_0037
DHA:	DEHA2D00990g
PIC:	PICST_36697(NIT1)
CLU:	CLUG_04100
NCR:	NCU05387
PAN:	PODANSg6829
MGR:	MGG_03280
NHE:	NECHADRAFT_30373
MAW:	MAC_09596
MAJ:	MAA_08327
SSL:	SS1G_10951
BFU:	BC1G_06521
ANI:	AN7367.2
AFM:	AFUA_6G13450
AOR:	AOR_1_826154(AO090003000470)
ANG:	ANI_1_1394104(An12g01260) ANI_1_254054(An06g01960)
AFV:	AFLA_033490
ACT:	ACLA_086750
NFI:	NFIA_059410
PCS:	Pc22g01000 Pc22g07020
CIM:	CIMG_10233
CPW:	CPC735_013280
PBL:	PAAG_04639
PBN:	PADG_04797
URE:	UREG_03497
PNO:	SNOG_02380
BZE:	COCCADRAFT_106140 COCCADRAFT_83227
TML:	GSTUM_00003299001
TBR:	Tb09.160.0770
TCR:	509979.150 510039.40
LMA:	LMJF_26_2280
LIF:	LINJ_26_2290
DDA:	Dd703_3697
DDD:	Dda3937_04568
DZE:	Dd1591_2288
PAO:	Pat9b_1901
FAU:	Fraau_0752
VEJ:	VEJY3_18251
PAP:	PSPA7_3027
PPU:	PP_4463
PPUH:	B479_13035
PFL:	PFL_2909
PPRC:	PFLCHA0_c29550(nitA)
PFO:	Pfl01_2571
PFS:	PFLU_2708
PFE:	PSF113_2665
PFC:	PflA506_2986
PPZ:	H045_09705
PEN:	PSEEN1080 PSEEN3513
PBA:	PSEBR_a3130
PPUU:	PputUW4_02136 PputUW4_02461
PAR:	Psyc_0202
PCR:	Pcryo_0222
SSE:	Ssed_0525
SPL:	Spea_2863
SHL:	Shal_2959
SVO:	SVI_3911
PHA:	PSHAa2676
PAT:	Patl_0189 Patl_2399
MAQ:	Maqu_2722
MBS:	MRBBS_2036
AMC:	MADE_1001325
GAG:	Glaag_4118
LPN:	lpg0950
LPF:	lpl0979
LPP:	lpp1012
LPC:	LPC_2337(nitA)
LPA:	lpa_01433(nitA)
LLO:	LLO_1945
MCA:	MCA0381
MMT:	Metme_0532
MAH:	MEALZ_3479
NOC:	Noc_2655
NHL:	Nhal_0547
NWA:	Nwat_0461
ALV:	Alvin_2521
AEH:	Mlg_0411
HHA:	Hhal_2135
TGR:	Tgr7_2268
TKM:	TK90_1987
HCH:	HCH_05328
KKO:	Kkor_0639
ASA:	ASA_2402
NMA:	NMA2044
NME:	NMB0441
NMC:	NMC1720
NMN:	NMCC_1705
NMI:	NMO_1599
NGK:	NGK_1800
NLA:	NLA_4840
CVI:	CV_2097
LHK:	LHK_02142
PSE:	NH8B_1273 NH8B_1744
RSO:	RSc2658
RSC:	RCFBP_10789
RSL:	RPSI07_0858 RPSI07_mp0987(nit)
RPI:	Rpic_2895
RPF:	Rpic12D_2489
REU:	Reut_A1028
REH:	H16_A1125(nit)
RME:	Rmet_0992(nit)
CTI:	RALTA_A1105
BMA:	BMA2336
BMV:	BMASAVP1_A0489
BML:	BMA10229_A1110
BMN:	BMA10247_2217
BMAL:	DM55_1447
BMAE:	DM78_2707
BMAQ:	DM76_1425
BMAI:	DM57_1113
BMAF:	DM51_1978
BMAZ:	BM44_1026
BPS:	BPSL2837
BPM:	BURPS1710b_3333(opaA)
BPL:	BURPS1106A_3322
BPD:	BURPS668_3289
BPR:	GBP346_A3467
BPSE:	BDL_2608
BPSM:	BBQ_473
BPSU:	BBN_600
BPSD:	BBX_1007
BPZ:	BP1026B_I0474
BPQ:	BPC006_I3371
BPK:	BBK_2093
BPSH:	DR55_1682
BPSA:	BBU_2725
BPSO:	X996_1321
BUT:	X994_3319
BTE:	BTH_I1297
BTQ:	BTQ_2636
BTJ:	BTJ_3059
BTZ:	BTL_989
BTD:	BTI_771
BTV:	BTHA_1080
BTHE:	BTN_398
BTHM:	BTRA_1197
BOK:	DM82_2508
BVI:	Bcep1808_0666 Bcep1808_0701
BVE:	AK36_140
BUR:	Bcep18194_A3790 Bcep18194_A3828
BCN:	Bcen_0220 Bcen_0256
BCH:	Bcen2424_0704 Bcen2424_0740
BCM:	Bcenmc03_0671 Bcenmc03_0710
BCJ:	BCAL3280 BCAL3323
BCEN:	DM39_513(nit2) DM39_549
BCEW:	DM40_1397(nit4) DM40_1432
BCEO:	I35_0552
BAM:	Bamb_0595 Bamb_0633
BAC:	BamMC406_0620 BamMC406_0658
BMU:	Bmul_2644 Bmul_2680
BMJ:	BMULJ_00558 BMULJ_00594
BMK:	DM80_927 DM80_966(nit2)
BCT:	GEM_2784 GEM_2822
BCED:	DM42_1097 DM42_1138(nit2)
BPYR:	ABD05_09095
BCON:	NL30_20595
BUB:	BW23_1032
BGL:	bglu_1g01570 bglu_1g06240
BGP:	BGL_1c06090(opaA)
BUG:	BC1001_0441
BGE:	BC1002_0496
BGF:	BC1003_0453
BGD:	bgla_1g01700 bgla_1g06650
BUK:	MYA_0610 MYA_0649
BUE:	BRPE67_ACDS03700
BXE:	Bxe_A3971
BXB:	DR64_1647
BPH:	Bphy_2508
BRH:	RBRH_01652
BPX:	BUPH_04330
BPY:	Bphyt_0727
PNU:	Pnuc_1777
BPE:	BP2910
BPC:	BPTD_2879
BPER:	BN118_2913
BPET:	B1917_0923
BPEU:	Q425_24480
BPA:	BPP2486
BPAR:	BN117_1812
BBR:	BB1116 BB1933
BBM:	BN115_1062 BN115_3104
BBH:	BN112_1582 BN112_2334
BBX:	BBS798_1084 BBS798_1834
BPT:	Bpet2740
AXY:	AXYL_01231(nit1) AXYL_01249(nit2)
PUT:	PT7_1546
RFR:	Rfer_0849
POL:	Bpro_3376 Bpro_4654
PNA:	Pnap_3896 Pnap_4743
AAV:	Aave_4655
AJS:	Ajs_4017
DIA:	Dtpsy_3367
AAA:	Acav_4464 Acav_4591
VEI:	Veis_0781
DAC:	Daci_0848
DEL:	DelCs14_5683
VAP:	Vapar_5158
VPE:	Varpa_5874
CTT:	CtCNB1_4747
ADN:	Alide_4241
ADK:	Alide2_4581
RTA:	Rta_37060
MPT:	Mpe_A0244
HAR:	HEAR0861
MMS:	mma_0836 mma_2480
HSE:	Hsero_1422
CFU:	CFU_3251(opaA)
LCH:	Lcho_3521
TIN:	Tint_0108
NEU:	NE1327
NET:	Neut_1264
NMU:	Nmul_A1056
TBD:	Tbd_0511
MFA:	Mfla_1745
MMB:	Mmol_1416
MEH:	M301_1672
MEI:	Msip34_1247
MEP:	MPQ_1319
SLT:	Slit_2094
GCA:	Galf_2048
EBA:	ebA970
DSU:	Dsui_3402
DAR:	Daro_3329
AZO:	azo1295
TMZ:	Tmz1t_1219
APP:	CAP2UW1_4066
CJE:	Cj1056c
CJJ:	CJJ81176_1076
CJR:	CJE1199
CFF:	CFF8240_0615
CCV:	CCV52592_0982
CHA:	CHAB381_0911
SFU:	Sfum_2312
MES:	Meso_0079
SFH:	SFHH103_06513(nit)
RLG:	Rleg_5502
OAN:	Oant_4151
BJA:	blr3397(nit)
RPA:	RPA4166
RPT:	Rpal_4645
RPX:	Rpdx1_4378
XAU:	Xaut_1567
AZC:	AZC_1804
MEA:	Mex_2p1120
MDI:	METDI2664
BID:	Bind_3461
MSL:	Msil_3811
PHL:	KKY_3824
CAK:	Caul_5248
RSP:	RSP_3399
RSH:	Rsph17029_3044
RSK:	RSKD131_3564
OAT:	OAN307_c22280
MMR:	Mmar10_1113
ZMO:	ZMO1207
ZMN:	Za10_0124
SWI:	Swit_2469
GBE:	GbCGDNIH1_0330
ACR:	Acry_3060
GDI:	GDI0018
GDJ:	Gdia_1775
ABS:	AZOBR_p350044(nitA)
MGM:	Mmc1_1403
AFE:	Lferr_0706
AFR:	AFE_0551
GMC:	GY4MC1_1997
GTH:	Geoth_2087
BSE:	Bsel_2856
BBE:	BBR47_51710
CKL:	CKL_2025
CKR:	CKR_1770
CDF:	CD630_28420
PDC:	CDIF630_03109(nit)
CDC:	CD196_2686
CDL:	CDR20291_2733
PDF:	CD630DERM_28420
NTH:	Nther_1072
HPK:	Hprae_1182
MED:	MELS_1676
AFN:	Acfer_0950
MSM:	MSMEG_1120 MSMEG_5373
MSG:	MSMEI_1088
NBR:	O3I_007265
RHA:	RHA1_ro03332 RHA1_ro03335 RHA1_ro03530
AAU:	AAur_0337
PDX:	Psed_2545
RXY:	Rxyl_0235
SYF:	Synpcc7942_0839
SYR:	SynRCC307_1156
SYX:	SynWH7803_1035
PMN:	PMN2A_0052
PMB:	A9601_06711
PMC:	P9515_06801
PMF:	P9303_18921
PMG:	P9301_06411 P9301_13131
PME:	NATL1_06731
PSL:	Psta_3064
SUS:	Acid_5967
SLI:	Slin_1649
RSI:	Runsl_5495
MTT:	Ftrac_1361
CAT:	CA2559_04585
FBC:	FB2170_08229
CAO:	Celal_0969
CLY:	Celly_0836
KDI:	Krodi_1527
LAN:	Lacal_0657
CPB:	Cphamn1_1310
HMA:	pNG7354(nitB)