KEGG   ENZYME: 4.1.99.1Help
Entry
EC 4.1.99.1                 Enzyme                                 

Name
tryptophanase;
L-tryptophanase;
L-tryptophan indole-lyase (deaminating);
TNase
Class
Lyases;
Carbon-carbon lyases;
Other carbon-carbon lyases
BRITE hierarchy
Sysname
L-tryptophan indole-lyase (deaminating; pyruvate-forming)
Reaction(IUBMB)
L-tryptophan + H2O = indole + pyruvate + NH3 (overall reaction) [RN:R00673];
(1a) L-tryptophan = indole + 2-aminoprop-2-enoate;
(1b) 2-aminoprop-2-enoate = 2-iminopropanoate (spontaneous);
(1c) 2-iminopropanoate + H2O = pyruvate + NH3 (spontaneous)
Reaction(KEGG)
Substrate
L-tryptophan [CPD:C00078];
H2O [CPD:C00001];
2-aminoprop-2-enoate [CPD:C02218];
2-iminopropanoate [CPD:C20904]
Product
indole [CPD:C00463];
pyruvate [CPD:C00022];
NH3 [CPD:C00014];
2-aminoprop-2-enoate [CPD:C02218];
2-iminopropanoate [CPD:C20904]
Comment
A pyridoxal-phosphate protein, requiring K+. The enzyme cleaves a carbon-carbon bond, releasing indole and an unstable enamine product that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form pyruvate and ammonia. The latter reaction, which can occur spontaneously, can also be catalysed by EC 3.5.99.10, 2-iminobutanoate/2-iminopropanoate deaminase. Also catalyses 2,3-elimination and beta-replacement reactions of some indole-substituted tryptophan analogues of L-cysteine, L-serine and other 3-substituted amino acids.
History
EC 4.1.99.1 created 1972
Pathway
ec00380  Tryptophan metabolism
Orthology
K01667  tryptophanase
Genes
PAN: PODANSg724
FVR: FVEG_14027
MAW: MAC_08116
MAJ: MAA_10321
ELA: UCREL1_916
EHI: EHI_111610(347.t00005)
EDI: EDI_311960
EIV: EIN_327340
ECO: b3708(tnaA)
ECJ: JW3686(tnaA)
ECD: ECDH10B_3895(tnaA)
EBW: BWG_3399(tnaA)
ECOK: ECMDS42_3145(tnaA)
ECE: Z5203(tnaA)
ECS: ECs4645(tnaA)
ECF: ECH74115_5139(tnaA)
ETW: ECSP_4756(tnaA)
ELX: CDCO157_4381(tnaA)
EOJ: ECO26_4874(tnaA)
EOI: ECO111_4535(tnaA)
EOH: ECO103_4450(tnaA)
ECG: E2348C_4019(tnaA)
EOK: G2583_4499(tnaA)
ELR: ECO55CA74_21605(tnaA)
ECC: c4631(tnaA)
ECP: ECP_3908
ECI: UTI89_C4261(tnaA)
ECV: APECO1_2752(tnaA)
ECX: EcHS_A3922(tnaA)
ECW: EcE24377A_4217(tnaA)
ECM: EcSMS35_4075(tnaA)
ECY: ECSE_3994
ECR: ECIAI1_3887(tnaA)
ECQ: ECED1_4399(tnaA)
ECK: EC55989_4178(tnaA)
ECT: ECIAI39_4312(tnaA)
EOC: CE10_4352(tnaA)
EUM: ECUMN_4239(tnaA)
ECZ: ECS88_4131(tnaA)
ELO: EC042_4065(tnaA)
ELN: NRG857_18470(tnaA)
ELH: ETEC_3999
ESE: ECSF_3553
ESO: O3O_00035(tnaA)
ESM: O3M_25225(tnaA)
ESL: O3K_25305(tnaA)
EBR: ECB_03592(tnaA)
EBD: ECBD_4324
EKF: KO11_03575(tnaA)
EAB: ECABU_c41910(tnaA)
EDJ: ECDH1ME8569_3596(tnaA)
EIH: ECOK1_4157(tnaA)
ENA: ECNA114_3857(tnaA)
ELU: UM146_18730(tnaA)
EUN: UMNK88_4517(tnaA)
ELW: ECW_m4007(tnaA)
ELL: WFL_19585(tnaA)
ELC: i14_4224(tnaA)
ELD: i02_4224(tnaA)
ELP: P12B_c3843(tnaA)
EBL: ECD_03592(tnaA)
EBE: B21_03536(tnaA)
ELF: LF82_2274(tnaA)
ECOL: LY180_19205(tnaA)
ECOI: ECOPMV1_04046(tnaA)
ECOJ: P423_20580(tnaA)
ECOO: ECRM13514_4774(tnaA)
ECOH: ECRM13516_4558(tnaA)
ECOS: EC958_4181(tnaA)
EFE: EFER_4004(tnaA)
EAL: EAKF1_ch2226c(tnaA)
EMA: C1192_16980(tnaA)
SFL: SF3754(tnaA)
SFX: S4017(tnaA)
SFV: SFV_3805(tnaA)
SFE: SFxv_4081(tnaA)
SFN: SFy_5348
SFS: SFyv_5419
SFT: NCTC1_04050(tnaA)
SBC: SbBS512_E4214(tnaA)
CCON: AFK62_02325(tnaA)
CDM: AFK67_02310(tnaA)
CMW: AFK63_16310(tnaA)
KOX: KOX_09730(tnaA)
KOE: A225_0649
KOY: J415_28015(tnaA)
KOK: KONIH1_03450(tnaA)
KOC: AB185_32730(tnaA)
KOM: HR38_08450(tnaA)
CKO: CKO_02849
CAMA: F384_20245(tnaA)
CAF: AL524_03030(tnaA)
CFAR: CI104_25255(tnaA)
CIR: C2U53_09720(tnaA)
ROR: RORB6_15790(tnaA)
CED: LH89_15965(tnaA)
KLE: AO703_10280(tnaA) AO703_20280(tnaA)
KGO: CEW81_05195(tnaA)
LAX: APT61_22105(tnaA)
LEI: C2U54_04835(tnaA)
LEH: C3F35_11655(tnaA)
YEN: YE0650(tnaA)
YEL: LC20_04527(tnaA)
YEW: CH47_2831(tnaA)
YET: CH48_953(tnaA)
YEE: YE5303_26881(tnaA)
YFR: AW19_3989(tnaA)
YIN: CH53_1040(tnaA)
YKR: CH54_1801(tnaA)
YMA: DA391_18765(tnaA)
SERA: Ser39006_006065(tnaA)
DDD: Dda3937_01355(tnaA)
DZC: W909_17045(tnaA)
DSO: A4U42_05600(tnaA)
DDQ: DDI_3576
DFN: CVE23_18965(tnaA)
PAM: PANA_0116(tnaA)
PLF: PANA5342_4311(tnaA)
PAJ: PAJ_3278(tnaA)
PSTW: DSJ_22365
PLU: plu0799(tnaA)
PTT: VY86_04110(tnaA)
XBV: XBW1_0706(tnaA)
XNE: XNC1_0444(tnaA) XNC1_2962(tnaA)
XNM: XNC2_0435(tnaA) XNC2_2841(tnaA)
XDO: XDD1_0413(tnaA)
PSI: S70_13785(tnaA)
PSX: DR96_507(tnaA)
PRG: RB151_038050(tnaA)
MMK: MU9_1101
ETR: ETAE_0843(tnaA)
ETD: ETAF_0789
ETE: ETEE_2719(tnaA)
ETC: ETAC_04050(tnaA)
EDW: QY76_15975(tnaA)
EDL: AAZ33_04480(tnaA)
PSHI: SAMEA2665130_0893(tnaA)
HIT: NTHI0831(tnaA)
HIU: HIB_08350
HIZ: R2866_1769(tnaA)
HIK: HifGL_000379(tnaA)
HIA: H733_0605
HIH: NF38_01615(tnaA)
HIC: NTHIC486_00286(tnaA)
HIX: NTHI723_00998(tnaA)
HSO: HS_0801(tnaA)
HSM: HSM_1270
PMU: PM1420(tnaA)
PMV: PMCN06_1678(tnaA)
PMP: Pmu_16710(tnaA)
PMUL: DR93_274(tnaA)
MVR: X781_900
VCH: VCA0161(tnaA)
VCF: IR04_00720(tnaA)
VCE: Vch1786_II0954(tnaA)
VCO: VC0395_1117(tnaA)
VCR: VC395_A0154(tnaA)
VCM: VCM66_A0159(tnaA)
VCI: O3Y_14228(tnaA)
VCQ: EN18_04510(tnaA)
VCS: MS6_A0141
VCX: VAA049_1753(tnaA)
VCZ: VAB027_874(tnaA)
VVU: VV2_0854(tnaA)
VVY: VVA1325
VVL: VV93_v1c42380(tnaA)
VPA: VPA0192(tnaA)
VPK: M636_00290(tnaA)
VPF: M634_19065(tnaA)
VCA: M892_18930(tnaA)
VAG: N646_3629
VHR: AL538_20685(tnaA)
VNA: PN96_15750(tnaA)
VRO: BSZ04_05365(tnaA)
VDB: AL552_02290(tnaA)
VSP: VS_1262
VEJ: VEJY3_23856(tnaA)
VNI: VIBNI_A0261(tnaA)
VAN: VAA_01202
LAG: N175_11350(tnaA)
VAU: VANGNB10_cI0734c(tnaA)
VCY: IX92_23840(tnaA) IX92_27170(tnaA)
VCT: JV59_24025(tnaA) JV59_25700(tnaA) JV59_30555(tnaA)
VTU: IX91_08130(tnaA) IX91_22075(tnaA) IX91_23675(tnaA)
VFL: AL536_07365(tnaA)
VMI: AL543_02320(tnaA)
AWD: AWOD_I_1599(tnaA)
GHO: AL542_02385(tnaA)
PSW: LK03_10530(tnaA)
SSE: Ssed_3916
SPSW: Sps_01497
MVS: MVIS_2967(tnaA)
AHA: AHA_1963(tnaA)
AHY: AHML_10600(tnaA)
AHD: AI20_09355(tnaA)
AHR: V428_10890(tnaA)
AHP: V429_10900(tnaA)
AHJ: V469_12505(tnaA)
AHH: RY45_10515(tnaA)
AHI: VU14_12410(tnaA)
ASA: ASA_2330(tnaA)
AVR: B565_2093
AVO: AMS64_10185(tnaA)
AMED: B224_5641
ADH: CK627_16240(tnaA)
ACAV: VI35_20810(tnaA)
AEM: CK911_07465(tnaA)
CVI: CV_1163(tnaA)
CVC: BKX93_18915(tnaA)
PSE: NH8B_1029
RFR: Rfer_2475
OFO: BRW83_0351(tpl)
RGE: RGE_20590(tnaA)
RBN: RBXJA2T_17611(tnaA)
EBA: ebA605(tnaA)
AZA: AZKH_4575(tnaA)
TCL: Tchl_2494
DVU: DVU2204(tnaA)
DVL: Dvul_1032
SAT: SYN_01455
RPA: RPA3564
RPB: RPB_1966
RPC: RPC_2787
RPD: RPD_3423
RPE: RPE_2915
RPT: Rpal_4083
RVA: Rvan_2623
BVR: BVIR_1448
MSC: BN69_2860
RCP: RCAP_rcc00516(tpl) RCAP_rcc02309(tnaA)
PSF: PSE_p0052(tpl)
RSU: NHU_02389(tnaA)
HNE: HNE_0810
MAGX: XM1_0733(tpl)
MAGN: WV31_12030
TMO: TMO_c0531(tnaA)
CTC: CTC_01509
CTET: BN906_01677(tnaA)
CACE: CACET_c32610(tnaA)
AMT: Amet_3003
HSC: HVS_04190(tpl)
STH: STH439(tna-2) STH441(tna-1)
DRM: Dred_2018
TTE: TTE1602(TnaA)
TAE: TepiRe1_2524(tnaA)
TOC: Toce_0634
PUF: UFO1_0888
PFT: JBW_04294
LPIL: LIP_0995
MBJ: KQ51_01254(tnaA)
CMD: B841_11865(tnaA)
CHM: B842_02140(tnaA)
CMQ: B840_02180(tnaA)
SRC: M271_29125(tnaA)
SMAL: SMALA_3214
KSK: KSE_04600(tnaA)
PAC: PPA1068
PAV: TIA2EST22_05315(tnaA)
PAX: TIA2EST36_05285(tnaA)
PAZ: TIA2EST2_05225(tnaA)
PAW: PAZ_c11120(tnaA)
PAD: TIIST44_09280(tnaA)
PCN: TIB1ST10_05480(tnaA)
PACC: PAC1_05600(tnaA)
PACH: PAGK_1083
PACN: TIA1EST1_05245(tnaA)
NCA: Noca_1624
SEN: SACE_1957
KAL: KALB_2889
ABAT: CFX1CAM_0064(tnaA)
CAP: CLDAP_10980(tnaA)
OTE: Oter_0376
AMU: Amuc_1995
SACI: Sinac_1688
TDE: TDE0251(tnaA)
TPED: TPE_1510(tnaA)
BHY: BHWA1_01919(tnaA)
BPW: WESB_0101(tnaA)
BIP: Bint_2781(tnaA)
SUS: Acid_6369
ABAC: LuPra_04134(tpl)
EMI: Emin_0932
FNU: FN1943(tnaA)
FVA: FV113G1_02940(tnaA)
TAI: Taci_0642
TLI: Tlie_1330
BTH: BT_1492(tnaA)
BTHO: Btheta7330_01722(tpl)
BXY: BXY_45090
BOA: Bovatus_01573(tnaA1)
BCEL: BcellWH2_01052(tpl)
BACC: BRDCF_p2216(tnaA)
PGI: PG_1401
PGN: PGN_0880
PGT: PGTDC60_2059(tnaA)
PMUC: ING2E5A_1495(tpl)
PSAC: PSM36_1292
AFD: Alfi_0891
ASH: AL1_23350
DORI: FH5T_08715
BLQ: L21SP5_01020(tpl)
MBAS: ALGA_2691
SRU: SRU_1708(tnaA)
SRM: SRM_01915(tnaA)
RMR: Rmar_1948
NSO: NIASO_14125(tnaA)
SMIZ: 4412673_01210(tpl_2)
SLI: Slin_0829
LBY: Lbys_1646
EAO: BD94_0046
EMN: M876_17465(tnaA)
ELB: VO54_02560(tpl)
CHZ: CHSO_0681(tnaA)
FBA: FIC_02450
IAL: IALB_2618(tnaA)
MRO: MROS_0698
PMO: Pmob_0309
DTN: DTL3_1682(tnaA)
NMV: NITMOv2_0067(tnaA)
NJA: NSJP_0536(tnaA)
HAL: VNG_2373G(tnaA)
HSL: OE_4331R(tnaA)
HDL: HALDL1_16665(tnaA)
HHB: Hhub_3742(tnaA)
HJE: HacjB3_08945(tnaA)
HALH: HTSR_0012
HHSR: HSR6_0012(tnaA)
HSU: HLASF_0008(tnaA)
HSF: HLASA_0008(tnaA)
HMA: rrnAC2439(tnaA)
HHI: HAH_2872(tnaA)
HHN: HISP_14600(tnaA)
HVO: HVO_0009(tnaA)
HME: HFX_0008(tnaA)
HTU: Htur_0018
NMG: Nmag_1937
NAT: NJ7G_0476
SALI: L593_14215(tnaA)
HLR: HALLA_19250(tnaA)
APE: APE_1275(tnaA)
 » show all
Taxonomy
Reference
1  [PMID:14017164]
  Authors
BURNS RO, DEMOSS RD.
  Title
Properties of tryptophanase from Escherichia coli.
  Journal
Biochim. Biophys. Acta. 65 (1962) 233-44.
Reference
2  [PMID:14284727]
  Authors
NEWTON WA, MORINO Y, SNELL EE.
  Title
PROPERTIES OF CRYSTALLINE TRYPTOPHANASE.
  Journal
J. Biol. Chem. 240 (1965) 1211-8.
Reference
3
  Authors
Cowell, J.L., Maser, K. and DeMoss, R.D.
  Title
Tryptophanase from Aeromonas liquifaciens. Purification, molecular weight and some chemical, catalytic and immunological properties.
  Journal
Biochim. Biophys. Acta 315 (1973) 449-463.
Reference
4  [PMID:236639]
  Authors
Snell EE
  Title
Tryptophanase: structure, catalytic activities, and mechanism of action.
  Journal
Adv. Enzymol. Relat. Areas. Mol. Biol. 42 (1975) 287-333.
Other DBs
ExplorEnz - The Enzyme Database: 4.1.99.1
IUBMB Enzyme Nomenclature: 4.1.99.1
ExPASy - ENZYME nomenclature database: 4.1.99.1
BRENDA, the Enzyme Database: 4.1.99.1
CAS: 9024-00-4
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