Entry
Name
Lipopolysaccharide biosynthesis - Photorhabdus laumondii subsp. laumondii TTO1
Description
Lipopolysaccharide (LPS) is the major component of the outer membrane of Gram-negative bacteria consisting of three domains: a hydrophobic anchor called lipid A, a non-repeating core oligosaccharide (OS) and a repetitive glycan polymer called O-antigen or O-polysaccharide (O-PS). The enzymes for the biosynthesis of lipid A are well conserved among Gram-negative bacterial species [MD:
M00060 M00866 ]. The core OS connecting lipid A with O-antigen is divided into inner and outer parts. Five core types, K-12 and R1-R4, are identified in Escherichia coli, with the common inner part composed of Kdo (ketodeoxyoctulosonic acid) and Hep (heptose) residues and the outer part made of various sugar residues. O-antigen is the most variable part used for serotyping of pathogenic Escherichia coli, where about 170 types are known. All three domains are involved in toxicity, pathogenicity, antimicrobial resistance and other activities.
Class
Metabolism; Glycan biosynthesis and metabolism
BRITE hierarchy
Pathway map
plu00540 Lipopolysaccharide biosynthesis
Ortholog table
Module
Other DBs
Organism
Photorhabdus laumondii subsp. laumondii TTO1 [GN:
plu ]
Gene
plu0684 lpxA; acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase [KO:K00677 ] [EC:2.3.1.129 ]
plu3648 lpxC; UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (UDP-3-O-acyl-GlcNAc deacetylase) (EnvA protein) [KO:K02535 ] [EC:3.5.1.108 ]
plu4036 unnamed protein product; Highly similar to putative isomerase YrbH of Escherichia coli [KO:K06041 ] [EC:5.3.1.13 ]
plu2073 kdsA; 2-dehydro-3-deoxyphosphooctonate aldolase (Phospho-2-dehydro-3-deoxyoctonate aldolase) (3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase) (KDO-8-phosphate synthetase) (KDO 8-P synthase) [KO:K01627 ] [EC:2.5.1.55 ]
plu4037 unnamed protein product; Highly similar to ABC transporter, permease protein YrbI of Escherichia coli [KO:K03270 ] [EC:3.1.3.45 ]
plu1634 kdsB; 3-deoxy-manno-octulosonate cytidylyltransferase (CMP-KDO synthetase) (CMP-2-keto-3-deoxyoctulosonic acid synthetase) (CKS) [KO:K00979 ] [EC:2.7.7.38 ]
plu1814 htrB; lipid A biosynthesis lauroyl acyltransferase (heat shock protein B) [KO:K02517 ] [EC:2.3.1.241 2.3.1.-]
plu2863 unnamed protein product; Similar to putative membrane protein YeiU of Escherichia coli [KO:K19803 ] [EC:2.7.4.29 ]
plu2784 unnamed protein product; Similar to putative exported protein of Yersinia pestis and some similarities with crcA protein of Escherichia coli [KO:K12973 ] [EC:2.3.1.251 ]
plu4847 rfaD; ADP-L-glycero-D-manno-heptose-6-epimerase (ADP-glyceromanno-heptose 6-epimerase) [KO:K03274 ] [EC:5.1.3.20 ]
plu4290 unnamed protein product; Similar to glycosyltransferase [KO:K19354 ] [EC:2.4.1.-]
plu4852 rfaG; Lipopolysaccharide core biosynthesis protein RfaG (Glucosyltransferase I) [KO:K02844 ] [EC:2.4.1.-]
plu0452 unnamed protein product; Similar to lipopolysaccharide 1,6-galactosyltransferase (UDP-D-galactose--(Glucosyl)lipopolysaccharide-alpha-1,3-D-galactosyltransferase), RfaB protein of Escherichia coli [KO:K02840 ] [EC:2.4.1.-]
Compound
C00043 UDP-N-acetyl-alpha-D-glucosamine
C01112 D-Arabinose 5-phosphate
C01187 3-Deoxy-D-manno-octulosonate
C04088 Octadecanoyl-[acyl-carrier protein]
C04121 CMP-3-deoxy-D-manno-octulosonate
C04478 3-Deoxy-D-manno-octulosonate 8-phosphate
C04652 UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine
C04738 UDP-3-O-(3-hydroxytetradecanoyl)-N-acetylglucosamine
C04919 2,3,2'3'-Tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate
C05223 Dodecanoyl-[acyl-carrier protein]
C05382 Sedoheptulose 7-phosphate
C06022 UDP-3-O-(3-hydroxytetradecanoyl)-D-glucosamine
C06024 3-Deoxy-D-manno-octulosonyl-lipid IV(A)
C06251 Lauroyl-KDO2-lipid IV(A)
C06397 ADP-D-glycero-beta-D-manno-heptose
C06398 ADP-L-glycero-beta-D-manno-heptose
C07836 D-glycero-beta-D-manno-Heptose 7-phosphate
C07838 D-glycero-beta-D-manno-Heptose 1-phosphate
C11472 D-glycero-beta-D-manno-Heptose 1,7-bisphosphate
C19877 4-O-Phospho-alpha-Kdo-(2->6)-lipid IVA
C19878 D-glycero-alpha-D-manno-Heptose 7-phosphate
C19879 D-glycero-alpha-D-manno-Heptose 1,7-bisphosphate
C19880 D-glycero-alpha-D-manno-Heptose 1-phosphate
C19881 GDP-D-glycero-alpha-D-manno-heptose
C19890 alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[4-P-L-Ara4N]-lipid A
C20933 (9Z)-Hexadec-9-enoyl-KDO2-lipid IV(A)
C21157 KDO2-lipid A 1-diphosphate
C21461 KDO2-lipid A 1-(2-aminoethyl diphosphate)
C21463 KDO2-lipid A 1,4'-bis(2-aminoethyl diphosphate)
C21991 Palmitoleoyl-myristoyl-KDO2-lipid A
C21992 3'-O-Deacylated KDO2-lipid A
C21994 1-Dephospho-KDO2-lipid A
C21997 4'-Dephosphorylated 1-PEtN-KDO-lipid A
C21999 Stearoyl-KDO2-lipid IVA
C22003 (S)-2-Hydroxymyristate-modified lipid A
C22068 H. pylori KDO2-lipid IVA
Reference
Authors
Heinrichs DE, Yethon JA, Whitfield C.
Title
Molecular basis for structural diversity in the core regions of the lipopolysaccharides of Escherichia coli and Salmonella enterica.
Journal
Reference
Authors
Kneidinger B, Marolda C, Graninger M, Zamyatina A, McArthur F, Kosma P, Valvano MA, Messner P.
Title
Biosynthesis pathway of ADP-L-glycero-beta-D-manno-heptose in Escherichia coli.
Journal
Reference
Authors
Wyckoff TJ, Raetz CR, Jackman JE.
Title
Antibacterial and anti-inflammatory agents that target endotoxin.
Journal
Reference
Authors
Shibayama K, Ohsuka S, Tanaka T, Arakawa Y, Ohta M.
Title
Conserved structural regions involved in the catalytic mechanism of Escherichia coli K-12 WaaO (RfaI).
Journal
Reference
Authors
Raetz CR, Whitfield C
Title
Lipopolysaccharide endotoxins.
Journal
Reference
Authors
Rubin EJ, O'Brien JP, Ivanov PL, Brodbelt JS, Trent MS
Title
Identification of a broad family of lipid A late acyltransferases with non-canonical substrate specificity.
Journal
Reference
Authors
Stead CM, Zhao J, Raetz CR, Trent MS
Title
Removal of the outer Kdo from Helicobacter pylori lipopolysaccharide and its impact on the bacterial surface.
Journal
Reference
Authors
Ebbensgaard A, Mordhorst H, Aarestrup FM, Hansen EB
Title
The Role of Outer Membrane Proteins and Lipopolysaccharides for the Sensitivity of Escherichia coli to Antimicrobial Peptides.
Journal
Reference
Authors
Botos I, Noinaj N, Buchanan SK
Title
Insertion of proteins and lipopolysaccharide into the bacterial outer membrane.
Journal
Related pathway
plu00520 Amino sugar and nucleotide sugar metabolism
KO pathway
LinkDB
All DBs