KEGG   PATHWAY: sbm03030
Entry
sbm03030                    Pathway                                
Name
DNA replication - Shewanella baltica OS185
Description
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
Class
Genetic Information Processing; Replication and repair
Pathway map
sbm03030  DNA replication
sbm03030

Organism
Shewanella baltica OS185 [GN:sbm]
Gene
Shew185_2344  DNA polymerase III, epsilon subunit [KO:K02342] [EC:2.7.7.7]
Shew185_2015  DNA polymerase III, epsilon subunit [KO:K02342] [EC:2.7.7.7]
Shew185_2671  Exonuclease RNase T and DNA polymerase III [KO:K02342] [EC:2.7.7.7]
Shew185_1460  DNA polymerase III, alpha subunit [KO:K02337] [EC:2.7.7.7]
Shew185_2615  DNA polymerase III, subunits gamma and tau [KO:K02343] [EC:2.7.7.7]
Shew185_2451  DNA polymerase III, delta prime subunit [KO:K02341] [EC:2.7.7.7]
Shew185_3313  DNA polymerase III, delta subunit [KO:K02340] [EC:2.7.7.7]
Shew185_3120  DNA polymerase III chi subunit HolC [KO:K02339] [EC:2.7.7.7]
Shew185_0002  DNA polymerase III, beta subunit [KO:K02338] [EC:2.7.7.7]
Shew185_0716  replicative DNA helicase [KO:K02314] [EC:5.6.2.3]
Shew185_1189  DNA primase [KO:K02316] [EC:2.7.7.101]
Shew185_0556  single-strand binding protein [KO:K03111]
Shew185_2014  Ribonuclease H [KO:K03469] [EC:3.1.26.4]
Shew185_1459  Ribonuclease H [KO:K03470] [EC:3.1.26.4]
Shew185_4345  DNA polymerase I [KO:K02335] [EC:2.7.7.7]
Shew185_2679  DNA ligase, NAD-dependent [KO:K01972] [EC:6.5.1.2]
Reference
PMID:8087839
  Authors
Stillman B.
  Title
Smart machines at the DNA replication fork.
  Journal
Cell 78:725-8 (1994)
DOI:10.1016/S0092-8674(94)90362-X
Reference
PMID:9759502
  Authors
Waga S, Stillman B.
  Title
The DNA replication fork in eukaryotic cells.
  Journal
Annu Rev Biochem 67:721-51 (1998)
DOI:10.1146/annurev.biochem.67.1.721
KO pathway
ko03030   
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