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Entry
spi03030                    Pathway                                

Name
DNA replication - Streptococcus pyogenes MGAS10750 (serotype M4)
Description
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
Class
Genetic Information Processing; Replication and repair
BRITE hierarchy
Pathway map
spi03030  DNA replication
spi03030

Ortholog table
Module
spi_M00260  DNA polymerase III complex, bacteria [PATH:spi03030]
Organism
Streptococcus pyogenes MGAS10750 (serotype M4) [GN:spi]
Gene
MGAS10750_Spy1640  dnaQ; DNA polymerase III, epsilon chain [KO:K02342] [EC:2.7.7.7]
MGAS10750_Spy1140  dnaE; DNA polymerase III alpha subunit [KO:K02337] [EC:2.7.7.7]
MGAS10750_Spy1766  polC; DNA polymerase III alpha subunit [KO:K03763] [EC:2.7.7.7]
MGAS10750_Spy1211  DNA polymerase III subunit gamma/tau [KO:K02343] [EC:2.7.7.7]
MGAS10750_Spy1212  DNA polymerase III subunit gamma/tau [KO:K02343] [EC:2.7.7.7]
MGAS10750_Spy0327  dnaX; DNA polymerase III, delta' subunit [KO:K02341] [EC:2.7.7.7]
MGAS10750_Spy1253  DNA polymerase III, delta subunit [KO:K02340] [EC:2.7.7.7]
MGAS10750_Spy0002  dnaN; DNA polymerase III, beta chain [KO:K02338] [EC:2.7.7.7]
MGAS10750_Spy1951  holB; Replicative DNA helicase [KO:K02314] [EC:3.6.4.12]
MGAS10750_Spy0686  dnaG; DNA primase [KO:K02316] [EC:2.7.7.-]
MGAS10750_Spy1613  ssb2; Single strand binding protein [KO:K03111]
MGAS10750_Spy0109  Single-strand DNA binding protein [KO:K03111]
MGAS10750_Spy1032  Ribonuclease HII [KO:K03470] [EC:3.1.26.4]
MGAS10750_Spy1623  Ribonuclease HIII [KO:K03471] [EC:3.1.26.4]
MGAS10750_Spy0165  polA; DNA polymerase I [KO:K02335] [EC:2.7.7.7]
MGAS10750_Spy0655  lig; NAD-dependent DNA ligase [KO:K01972] [EC:6.5.1.2]
Reference
PMID:8087839
  Authors
Stillman B.
  Title
Smart machines at the DNA replication fork.
  Journal
Cell 78:725-8 (1994)
DOI:10.1016/S0092-8674(94)90362-X
Reference
PMID:9759502
  Authors
Waga S, Stillman B.
  Title
The DNA replication fork in eukaryotic cells.
  Journal
Annu Rev Biochem 67:721-51 (1998)
DOI:10.1146/annurev.biochem.67.1.721
KO pathway
ko03030   
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