KEGG   PATHWAY: vsp03030
Entry
vsp03030                    Pathway                                
Name
DNA replication - Vibrio atlanticus
Description
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
Class
Genetic Information Processing; Replication and repair
Pathway map
vsp03030  DNA replication
vsp03030

Organism
Vibrio atlanticus [GN:vsp]
Gene
VS_2941  putative DNA polymerase III, epsilon subunit [KO:K02342] [EC:2.7.7.7]
VS_1822  DNA polymerase III polC-type [KO:K02342] [EC:2.7.7.7]
VS_II1160  DNA polymerase III, alpha subunit [KO:K02342] [EC:2.7.7.7]
VS_1253  putative exonuclease [KO:K02342] [EC:2.7.7.7]
VS_2324  DNA polymerase III, epsilon subunit [KO:K02342] [EC:2.7.7.7]
VS_2337  DNA polymerase III alpha subunit [KO:K02337] [EC:2.7.7.7]
VS_0915  DNA polymerase III subunit tau [KO:K02343] [EC:2.7.7.7]
VS_1030  DNA polymerase III delta' subunit [KO:K02341] [EC:2.7.7.7]
VS_0716  DNA polymerase III, delta subunit [KO:K02340] [EC:2.7.7.7]
VS_2469  Similar to DNA polymerase III, psi subunit [KO:K02344] [EC:2.7.7.7]
VS_2731  DNA polymerase III chi subunit [KO:K02339] [EC:2.7.7.7]
VS_0011  DNA polymerase III beta subunit [KO:K02338] [EC:2.7.7.7]
VS_0284  Replicative DNA helicase [KO:K02314] [EC:5.6.2.3]
VS_0398  DNA primase [KO:K02316] [EC:2.7.7.101]
VS_0323  Single-strand binding protein (SSB) (Helix-destabilizing protein) [KO:K03111]
VS_2325  Ribonuclease HI [KO:K03469] [EC:3.1.26.4]
VS_1653  putative Ribonuclease HI [KO:K03469] [EC:3.1.26.4]
VS_2153  Ribonuclease HI [KO:K03469] [EC:3.1.26.4]
VS_2338  Ribonuclease HII [KO:K03470] [EC:3.1.26.4]
VS_0108  DNA polymerase I [KO:K02335] [EC:2.7.7.7]
VS_2286  DNA ligase [KO:K01972] [EC:6.5.1.2]
Reference
PMID:8087839
  Authors
Stillman B.
  Title
Smart machines at the DNA replication fork.
  Journal
Cell 78:725-8 (1994)
DOI:10.1016/S0092-8674(94)90362-X
Reference
PMID:9759502
  Authors
Waga S, Stillman B.
  Title
The DNA replication fork in eukaryotic cells.
  Journal
Annu Rev Biochem 67:721-51 (1998)
DOI:10.1146/annurev.biochem.67.1.721
KO pathway
ko03030   
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