| Entry |
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| Name |
2-hydroxy-4-carboxymuconate semialdehyde hemiacetal dehydrogenase;
2-hydroxy-4-carboxymuconate 6-semialdehyde dehydrogenase;
4-carboxy-2-hydroxy-cis,cis-muconate-6-semialdehyde:NADP+ oxidoreductase;
alpha-hydroxy-gamma-carboxymuconic epsilon-semialdehyde dehydrogenase;
4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase;
LigC;
ProD
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| Class |
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
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| Sysname |
4-carboxy-2-hydroxymuconate semialdehyde hemiacetal:NADP+ 2-oxidoreductase
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| Reaction(IUBMB) |
4-carboxy-2-hydroxymuconate semialdehyde hemiacetal + NADP+ = 2-oxo-2H-pyran-4,6-dicarboxylate + NADPH + H+ [RN: R04279]
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| Reaction(KEGG) |
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| Substrate |
4-carboxy-2-hydroxymuconate semialdehyde hemiacetal;
NADP+ [CPD: C00006]
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| Product |
2-oxo-2H-pyran-4,6-dicarboxylate;
NADPH [CPD: C00005];
H+ [CPD: C00080]
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| Comment |
The enzyme does not act on unsubstituted aliphatic or aromatic aldehydes or glucose; NAD+ can replace NADP+, but with lower affinity. The enzyme was initially believed to act on 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde and produce 4-carboxy-2-hydroxy-cis,cis-muconate [1]. However, later studies showed that the substrate is the hemiacetal form [3], and the product is 2-oxo-2H-pyran-4,6-dicarboxylate [2,4].
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| History |
EC 1.1.1.312 created 1978 as EC 1.2.1.45, transferred 2011 to EC 1.1.1.312
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| Pathway |
| ec01120 | Microbial metabolism in diverse environments |
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| Orthology |
| K10219 | 2-hydroxy-4-carboxymuconate semialdehyde hemiacetal dehydrogenase |
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| Genes |
» show all
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| Reference |
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| Authors |
Maruyama K, Ariga N, Tsuda M, Deguchi K. |
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| Title |
Purification and properties of alpha-hydroxy-gamma-carboxymuconic epsilon-semialdehyde dehydrogenase. |
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| Journal |
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| Reference |
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| Authors |
Maruyama K |
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| Title |
Isolation and identification of the reaction product of alpha-hydroxy-gamma-carboxymuconic epsilon-semialdehyde dehydrogenase. |
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| Journal |
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| Reference |
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| Authors |
Maruyama K. |
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| Title |
Purification and properties of 2-pyrone-4,6-dicarboxylate hydrolase. |
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| Journal |
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| Reference |
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| Authors |
Masai E, Momose K, Hara H, Nishikawa S, Katayama Y, Fukuda M |
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| Title |
Genetic and biochemical characterization of 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase and its role in the protocatechuate 4,5-cleavage pathway in Sphingomonas paucimobilis SYK-6. |
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| Journal |
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| Sequence |
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| Other DBs |
| ExPASy - ENZYME nomenclature database: | 1.1.1.312 |
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| LinkDB |
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