KEGG   ENZYME: 1.1.3.23
Entry
EC 1.1.3.23                 Enzyme                                 

Name
thiamine oxidase;
thiamin dehydrogenase;
thiamine dehydrogenase;
thiamin:oxygen 5-oxidoreductase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With oxygen as acceptor
Sysname
thiamine:oxygen 5-oxidoreductase
Reaction(IUBMB)
thiamine + 2 O2 + H2O = thiamine acetic acid + 2 H2O2 [RN:R02132]
Reaction(KEGG)
R02132;
(other) R00033 R00072
Substrate
thiamine [CPD:C00378];
O2 [CPD:C00007];
H2O [CPD:C00001]
Product
thiamine acetic acid [CPD:C02892];
H2O2 [CPD:C00027]
Comment
A flavoprotein (FAD). The product differs from thiamine in replacement of -CH2.CH2.OH by -CH2.COOH; the two-step oxidation proceeds without the release of the intermediate aldehyde from the enzyme.
History
EC 1.1.3.23 created 1984
Pathway
ec00730  Thiamine metabolism
ec01100  Metabolic pathways
Reference
1  [PMID:8076]
  Authors
Edmondson DE, Kenney WC, Singer TP.
  Title
Structural elucidation and properties of 8alpha-(N1-histidyl)riboflavin: the flavin component of thiamine dehydrogenase and beta-cyclopiazonate oxidocyclase.
  Journal
Biochemistry 15:2937-45 (1976)
DOI:10.1021/bi00659a001
Reference
2  [PMID:2988447]
  Authors
Gomez-Moreno C, Edmondson DE.
  Title
Evidence for an aldehyde intermediate in the catalytic mechanism of thiamine oxidase.
  Journal
Arch Biochem Biophys 239:46-52 (1985)
DOI:10.1016/0003-9861(85)90810-0
Reference
3  [PMID:4987737]
  Authors
Neal RA.
  Title
Bacterial metabolism of thiamine. 3. Metabolism of thiamine to 3-(2'-methyl-4'-amino-5'-pyrimidylmethyl)-4-methyl-thiazole-5-acetic acid (thiamine acetic acid) by a flavoprotein isolated from a soil microorganism.
  Journal
J Biol Chem 245:2599-604 (1970)
Other DBs
ExplorEnz - The Enzyme Database: 1.1.3.23
IUBMB Enzyme Nomenclature: 1.1.3.23
ExPASy - ENZYME nomenclature database: 1.1.3.23
BRENDA, the Enzyme Database: 1.1.3.23
CAS: 96779-44-1
LinkDB

KEGG   REACTION: R00072
Entry
R00072                      Reaction                               

Name
thiamine-aldehyde:oxygen 5-oxidoreductase
Definition
Thiamine aldehyde + Oxygen + H2O <=> Hydrogen peroxide + Thiamine acetic acid
Equation
Comment
second step of two-step reaction (see R02132, R00033+R00072)
following after R00033
Reaction class
RC00080  C02892_C05856
RC02755  C00007_C00027
Enzyme
Pathway
rn00730  Thiamine metabolism
rn01100  Metabolic pathways
Reference
1  [PMID:2988447]
  Authors
Gomez-Moreno C, Edmondson DE.
  Title
Evidence for an aldehyde intermediate in the catalytic mechanism of thiamine oxidase.
  Journal
Arch Biochem Biophys 239:46-52 (1985)
DOI:10.1016/0003-9861(85)90810-0
LinkDB

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