KEGG   ENZYME: 1.13.11.29
Entry
EC 1.13.11.29               Enzyme                                 

Name
stizolobate synthase
Class
Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of two atoms of oxygen
Sysname
3,4-dihydroxy-L-phenylalanine:oxygen 4,5-oxidoreductase (recyclizing)
Reaction(IUBMB)
L-dopa + O2 = 4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde [RN:R02075]
Reaction(KEGG)
R02075;
(other) R04598 R04599
Substrate
L-dopa [CPD:C00355];
O2 [CPD:C00007]
Product
4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde [CPD:C04796]
Comment
The intermediate product undergoes ring closure and oxidation, with NAD(P)+ as acceptor, to stizolobic acid. The enzyme requires Zn2+.
History
EC 1.13.11.29 created 1978
Pathway
ec00350  Tyrosine metabolism
Reference
1  [PMID:9285]
  Authors
Saito K, Komamine A.
  Title
Biosynthesis of stizolobinic acid and stizolobic acid in higher plants. An enzyme system(s) catalyzing the conversion of dihydroxyphenylalanine into stizolobinic acid and stizolobic acid from etiolated seedlings of Stizolobium hassjoo.
  Journal
Eur J Biochem 68:237-43 (1976)
DOI:10.1111/j.1432-1033.1976.tb10783.x
Reference
2  [PMID:624278]
  Authors
Saito K, Komamine A.
  Title
Biosynthesis of stizolobinic acid and stizolobic acid in higher plants.
  Journal
Eur J Biochem 82:385-92 (1978)
DOI:10.1111/j.1432-1033.1978.tb12033.x
Other DBs
ExplorEnz - The Enzyme Database: 1.13.11.29
IUBMB Enzyme Nomenclature: 1.13.11.29
ExPASy - ENZYME nomenclature database: 1.13.11.29
BRENDA, the Enzyme Database: 1.13.11.29
CAS: 65979-39-7
LinkDB

KEGG   REACTION: R04598
Entry
R04598                      Reaction                               

Name
3,4-Dihydroxy-L-phenylalanine:oxygen 4,5-oxidoreductase (recyclizing)
Definition
Stizolobate + NADH + H+ <=> 4-(L-Alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde + NAD+
Equation
Comment
possibly two-step reaction
following after R02075
Reaction class
RC00001  C00003_C00004
RC00259  C04796_C06047
Enzyme
Pathway
rn00350  Tyrosine metabolism
LinkDB

KEGG   REACTION: R04599
Entry
R04599                      Reaction                               

Name
3,4-Dihydroxy-L-phenylalanine:oxygen 4,5-oxidoreductase (recyclizing)
Definition
Stizolobate + NADPH + H+ <=> 4-(L-Alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+
Equation
Comment
possibly two-step reaction
following after R02075
Reaction class
RC00001  C00005_C00006
RC00259  C04796_C06047
Enzyme
Pathway
rn00350  Tyrosine metabolism
LinkDB

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