KEGG   ENZYME: 1.13.11.30
Entry
EC 1.13.11.30               Enzyme                                 

Name
stizolobinate synthase
Class
Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of two atoms of oxygen
Sysname
3,4-dihydroxy-L-phenylalanine:oxygen 2,3-oxidoreductase (recyclizing)
Reaction(IUBMB)
L-dopa + O2 = 5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde [RN:R02076]
Reaction(KEGG)
R02076;
(other) R04600 R04601
Substrate
L-dopa [CPD:C00355];
O2 [CPD:C00007]
Product
5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde [CPD:C04797]
Comment
The intermediate product undergoes ring closure and oxidation, with NAD(P)+ as acceptor, to stizolobinic acid. The enzyme requires Zn2+.
History
EC 1.13.11.30 created 1978
Pathway
ec00350  Tyrosine metabolism
Reference
1  [PMID:9285]
  Authors
Saito K, Komamine A.
  Title
Biosynthesis of stizolobinic acid and stizolobic acid in higher plants. An enzyme system(s) catalyzing the conversion of dihydroxyphenylalanine into stizolobinic acid and stizolobic acid from etiolated seedlings of Stizolobium hassjoo.
  Journal
Eur J Biochem 68:237-43 (1976)
DOI:10.1111/j.1432-1033.1976.tb10783.x
Reference
2  [PMID:624278]
  Authors
Saito K, Komamine A.
  Title
Biosynthesis of stizolobinic acid and stizolobic acid in higher plants.
  Journal
Eur J Biochem 82:385-92 (1978)
DOI:10.1111/j.1432-1033.1978.tb12033.x
Other DBs
ExplorEnz - The Enzyme Database: 1.13.11.30
IUBMB Enzyme Nomenclature: 1.13.11.30
ExPASy - ENZYME nomenclature database: 1.13.11.30
BRENDA, the Enzyme Database: 1.13.11.30
CAS: 65979-38-6
LinkDB

KEGG   REACTION: R04600
Entry
R04600                      Reaction                               

Name
3,4-Dihydroxy-L-phenylalanine:oxygen 2,3-oxidoreductase (recyclizing)
Definition
Stizolobinate + NADH + H+ <=> 5-(L-Alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde + NAD+
Equation
Comment
possibly two-step reaction
following after R02076
Reaction class
RC00001  C00003_C00004
RC00260  C04797_C06048
Enzyme
Pathway
rn00350  Tyrosine metabolism
LinkDB

KEGG   REACTION: R04601
Entry
R04601                      Reaction                               

Name
3,4-Dihydroxy-L-phenylalanine:oxygen 2,3-oxidoreductase (recyclizing)
Definition
Stizolobinate + NADPH + H+ <=> 5-(L-Alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+
Equation
Comment
possibly two-step reaction
following after R02076
Reaction class
RC00001  C00005_C00006
RC00260  C04797_C06048
Enzyme
Pathway
rn00350  Tyrosine metabolism
LinkDB

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