| Entry |
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| Name |
4-hydroxyphenylacetate 3-monooxygenase;
p-hydroxyphenylacetate 3-hydroxylase;
4-hydroxyphenylacetic acid-3-hydroxylase;
p-hydroxyphenylacetate hydroxylase (FAD);
4 HPA 3-hydroxylase;
p-hydroxyphenylacetate 3-hydroxylase (FAD);
HpaB
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| Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
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| Sysname |
4-hydroxyphenylacetate,FADH2:oxygen oxidoreductase (3-hydroxylating)
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| Reaction(IUBMB) |
4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O
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| Reaction(KEGG) |
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| Substrate |
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| Product |
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| Comment |
The enzyme from Escherichia coli attacks a broad spectrum of phenolic compounds. The enzyme uses FADH2 as a substrate rather than a cofactor [4]. FADH2 is provided by EC 1.5.1.36, flavin reductase (NADH) [5,6].
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| History |
EC 1.14.14.9 created 1972 as EC 1.14.13.3, transferred 2011 to EC 1.14.14.9
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| Pathway |
| ec01120 | Microbial metabolism in diverse environments |
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| Orthology |
| K00483 | 4-hydroxyphenylacetate 3-monooxygenase |
| K16901 | anthranilate 3-monooxygenase (FAD) / 4-hydroxyphenylacetate 3-monooxygenase |
| K23492 | 4-hydroxyphenylacetate 3-monooxygenase |
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| Genes |
» show all
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| Reference |
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| Authors |
ADACHI K, TAKEDA Y, SENOH S, KITA H. |
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| Title |
METABOLISM OF P-HYDROXYPHENYLACETIC ACID IN PSEUDOMONAS OVALIS. |
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| Journal |
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| Reference |
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| Authors |
Prieto MA, Perez-Aranda A, Garcia JL |
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| Title |
Characterization of an Escherichia coli aromatic hydroxylase with a broad substrate range. |
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| Journal |
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| Reference |
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| Authors |
Prieto MA, Garcia JL |
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| Title |
Molecular characterization of 4-hydroxyphenylacetate 3-hydroxylase of Escherichia coli. A two-protein component enzyme. |
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| Journal |
J Biol Chem 269:22823-9 (1994) |
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| Sequence |
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| Reference |
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| Authors |
Xun L, Sandvik ER |
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| Title |
Characterization of 4-hydroxyphenylacetate 3-hydroxylase (HpaB) of Escherichia coli as a reduced flavin adenine dinucleotide-utilizing monooxygenase. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Galan B, Diaz E, Prieto MA, Garcia JL |
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| Title |
Functional analysis of the small component of the 4-hydroxyphenylacetate 3-monooxygenase of Escherichia coli W: a prototype of a new Flavin:NAD(P)H reductase subfamily. |
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| Journal |
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| Reference |
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| Authors |
Louie TM, Xie XS, Xun L |
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| Title |
Coordinated production and utilization of FADH2 by NAD(P)H-flavin oxidoreductase and 4-hydroxyphenylacetate 3-monooxygenase. |
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| Journal |
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| Other DBs |
| ExPASy - ENZYME nomenclature database: | 1.14.14.9 |
| UM-BBD (Biocatalysis/Biodegradation Database): | 1.14.14.9 |
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| LinkDB |
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