KEGG   ORTHOLOGY: K00434
Entry
K00434                      KO                                     

Name
E1.11.1.11
Definition
L-ascorbate peroxidase [EC:1.11.1.11]
Pathway
ko00053  Ascorbate and aldarate metabolism
ko00480  Glutathione metabolism
ko01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09101 Carbohydrate metabolism
   00053 Ascorbate and aldarate metabolism
    K00434  E1.11.1.11; L-ascorbate peroxidase
  09106 Metabolism of other amino acids
   00480 Glutathione metabolism
    K00434  E1.11.1.11; L-ascorbate peroxidase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.11  Acting on a peroxide as acceptor
   1.11.1  Peroxidases
    1.11.1.11  L-ascorbate peroxidase
     K00434  E1.11.1.11; L-ascorbate peroxidase
Other DBs
RN: R00644 R09540
GO: 0016688
Genes
HMG: 100213023
ATH: AT1G07890(APX1) AT1G77490(TAPX) AT3G09640(APX2) AT4G08390(SAPX) AT4G09010(TL29) AT4G32320(APX6) AT4G35000(APX3) AT4G35970(APX5)
ALY: 9303117 9303168 9305354 9308432 9310603 9318697 9322135 9322136 9325208 9328486
CRB: 17878438 17879152 17880619 17884052 17884559 17890815 17893627 17893747 17896270 17899405
CSAT: 104702532 104707160 104707215 104713416 104716524 104716637 104716949 104719794 104720283 104721287 104729589 104729717 104730042 104736879 104736925 104739373 104745137 104747469 104747470 104751645 104754395 104754965 104764660 104765991 104766972 104766975 104792024 104792033
EUS: EUTSA_v10008402mg EUTSA_v10018581mg EUTSA_v10021381mg EUTSA_v10022797mg EUTSA_v10023023mg EUTSA_v10025686mg EUTSA_v10025911mg EUTSA_v10025942mg EUTSA_v10028739mg EUTSA_v10028759mg
BRP: 103832195 103836413 103838791 103843426 103848043 103858501 103860476 103862119 103862385 103871533 103871729
BNA: 106346376 106346377 106347996 106354606 106362267 106386647 106389449 106401842 106407140 106415142 106419633 106420215 106422138 106423276 106427935 106428784 106433208 106435662 106435671 106438471 106439834 106446154 106446926 111199406 111207743
BOE: 106294764 106300129 106303893 106306187 106307224 106308404 106312994 106324757 106324893 106328411 106328985
RSZ: 108812703 108814310 108814761 108814785 108820821 108822828 108824179 108837581 108844696 108848897 108851335 108851337 108856542 108856708
LJA: Lj1g3v0250130.2(Lj1g3v0250130.2) Lj1g3v0839140.1(Lj1g3v0839140.1) Lj1g3v0839140.2(Lj1g3v0839140.2) Lj1g3v1901620.1(Lj1g3v1901620.1) Lj3g3v3465510.1(Lj3g3v3465510.1)
DOSA: Os02t0553200-01(Os02g0553200) Os03t0285700-01(Os03g0285700) Os04t0223300-00(Os04g0223300) Os04t0434800-01(Os04g0434800) Os04t0602100-01(Os04g0602100) Os07t0694700-01(Os07g0694700) Os08t0522400-01(Os08g0522400) Os08t0549100-01(Os08g0549100) Os12t0178100-01(Os12g0178100) Os12t0178200-01(Os12g0178200)
ATS: 109739529(LOC109739529) 109747802(LOC109747802) 109748346(LOC109748346) 109749043(LOC109749043) 109749624(LOC109749624) 109757934(LOC109757934) 109771362(LOC109771362) 109785628(LOC109785628) 109786189(LOC109786189)
SMIN: v1.2.006460.t2(symbB.v1.2.006460.t2) v1.2.015684.t1(symbB.v1.2.015684.t1) v1.2.018236.t1(symbB.v1.2.018236.t1) v1.2.018679.t1(symbB.v1.2.018679.t1) v1.2.024207.t2(symbB.v1.2.024207.t2) v1.2.024207.t3(symbB.v1.2.024207.t3) v1.2.028328.t1(symbB.v1.2.028328.t1) v1.2.032093.t1(symbB.v1.2.032093.t1)
LMA: LMJF_34_0070(APX)
LIF: LINJ_34_0070(APX)
LBZ: LBRM_20_0150(APX)
LPAN: LPMP_200320(APX)
 » show all
Reference
PMID:12351682 (T.cruzi)
  Authors
Wilkinson SR, Obado SO, Mauricio IL, Kelly JM
  Title
Trypanosoma cruzi expresses a plant-like ascorbate-dependent hemoperoxidase localized to the endoplasmic reticulum.
  Journal
Proc Natl Acad Sci U S A 99:13453-8 (2002)
DOI:10.1073/pnas.202422899
Reference
PMID:18460785 (A.thaliana)
  Authors
Ishikawa T, Shigeoka S
  Title
Recent advances in ascorbate biosynthesis and the physiological significance of ascorbate peroxidase in photosynthesizing organisms.
  Journal
Biosci Biotechnol Biochem 72:1143-54 (2008)
DOI:10.1271/bbb.80062
Reference
PMID:16784232 (G.max)
  Authors
Macdonald IK, Badyal SK, Ghamsari L, Moody PC, Raven EL
  Title
Interaction of ascorbate peroxidase with substrates: a mechanistic and structural analysis.
  Journal
Biochemistry 45:7808-17 (2006)
DOI:10.1021/bi0606849
  Sequence
[gmx:553156]
LinkDB

KEGG   ENZYME: 1.11.1.11
Entry
EC 1.11.1.11                Enzyme                                 

Name
L-ascorbate peroxidase;
L-ascorbic acid peroxidase;
L-ascorbic acid-specific peroxidase;
ascorbate peroxidase;
ascorbic acid peroxidase
Class
Oxidoreductases;
Acting on a peroxide as acceptor;
Peroxidases
Sysname
L-ascorbate:hydrogen-peroxide oxidoreductase
Reaction(IUBMB)
2 L-ascorbate + H2O2 + 2 H+ = L-ascorbate + L-dehydroascorbate + 2 H2O (overall reaction) [RN:R00644];
(1a) 2 L-ascorbate + H2O2 + 2 H+ = 2 monodehydroascorbate + 2 H2O [RN:R09540];
(1b) 2 monodehydroascorbate = L-ascorbate + L-dehydroascorbate (spontaneous) [RN:R03186]
Reaction(KEGG)
Substrate
L-ascorbate [CPD:C00072];
H2O2 [CPD:C00027];
H+ [CPD:C00080];
monodehydroascorbate [CPD:C01041]
Product
L-ascorbate [CPD:C00072];
L-dehydroascorbate [CPD:C05422];
H2O [CPD:C00001];
monodehydroascorbate [CPD:C01041]
Comment
A heme protein. Oxidizes ascorbate and low molecular weight aromatic substrates. The monodehydroascorbate radical produced is either directly reduced back to ascorbate by EC 1.6.5.4 [monodehydroascorbate reductase (NADH)] or undergoes non-enzymic disproportionation to ascorbate and dehydroascorbate.
History
EC 1.11.1.11 created 1983, modified 2010, modified 2011
Pathway
ec00053  Ascorbate and aldarate metabolism
ec00480  Glutathione metabolism
ec01100  Metabolic pathways
Orthology
K00434  L-ascorbate peroxidase
Genes
HMG: 100213023
ATH: AT1G07890(APX1) AT1G77490(TAPX) AT3G09640(APX2) AT4G08390(SAPX) AT4G09010(TL29) AT4G32320(APX6) AT4G35000(APX3) AT4G35970(APX5)
ALY: 9303117 9303168 9305354 9308432 9310603 9318697 9322135 9322136 9325208 9328486
CRB: 17878438 17879152 17880619 17884052 17884559 17890815 17893627 17893747 17896270 17899405
CSAT: 104702532 104707160 104707215 104713416 104716524 104716637 104716949 104719794 104720283 104721287 104729589 104729717 104730042 104736879 104736925 104739373 104745137 104747469 104747470 104751645 104754395 104754965 104764660 104765991 104766972 104766975 104792024 104792033
EUS: EUTSA_v10008402mg EUTSA_v10018581mg EUTSA_v10021381mg EUTSA_v10022797mg EUTSA_v10023023mg EUTSA_v10025686mg EUTSA_v10025911mg EUTSA_v10025942mg EUTSA_v10028739mg EUTSA_v10028759mg
BRP: 103832195 103836413 103838791 103843426 103848043 103858501 103860476 103862119 103862385 103871533 103871729
BNA: 106346376 106346377 106347996 106354606 106362267 106386647 106389449 106401842 106407140 106415142 106419633 106420215 106422138 106423276 106427935 106428784 106433208 106435662 106435671 106438471 106439834 106446154 106446926 111199406 111207743
BOE: 106294764 106300129 106303893 106306187 106307224 106308404 106312994 106324757 106324893 106328411 106328985
RSZ: 108812703 108814310 108814761 108814785 108820821 108822828 108824179 108837581 108844696 108848897 108851335 108851337 108856542 108856708
LJA: Lj1g3v0250130.2(Lj1g3v0250130.2) Lj1g3v0839140.1(Lj1g3v0839140.1) Lj1g3v0839140.2(Lj1g3v0839140.2) Lj1g3v1901620.1(Lj1g3v1901620.1) Lj3g3v3465510.1(Lj3g3v3465510.1)
DOSA: Os02t0553200-01(Os02g0553200) Os03t0285700-01(Os03g0285700) Os04t0223300-00(Os04g0223300) Os04t0434800-01(Os04g0434800) Os04t0602100-01(Os04g0602100) Os07t0694700-01(Os07g0694700) Os08t0522400-01(Os08g0522400) Os08t0549100-01(Os08g0549100) Os12t0178100-01(Os12g0178100) Os12t0178200-01(Os12g0178200)
ATS: 109739529(LOC109739529) 109747802(LOC109747802) 109748346(LOC109748346) 109749043(LOC109749043) 109749624(LOC109749624) 109757934(LOC109757934) 109771362(LOC109771362) 109785628(LOC109785628) 109786189(LOC109786189)
SMIN: v1.2.006460.t2(symbB.v1.2.006460.t2) v1.2.015684.t1(symbB.v1.2.015684.t1) v1.2.018236.t1(symbB.v1.2.018236.t1) v1.2.018679.t1(symbB.v1.2.018679.t1) v1.2.024207.t2(symbB.v1.2.024207.t2) v1.2.024207.t3(symbB.v1.2.024207.t3) v1.2.028328.t1(symbB.v1.2.028328.t1) v1.2.032093.t1(symbB.v1.2.032093.t1)
LMA: LMJF_34_0070(APX)
LIF: LINJ_34_0070(APX)
LBZ: LBRM_20_0150(APX)
LPAN: LPMP_200320(APX)
 » show all
Reference
1  [PMID:6772104]
  Authors
Shigeoka S, Nakano Y, Kitaoka S.
  Title
Purification and some properties of L-ascorbic-acid-specific peroxidase in Euglena gracilis Z.
  Journal
Arch Biochem Biophys 201:121-7 (1980)
DOI:10.1016/0003-9861(80)90495-6
Reference
2  [PMID:6768357]
  Authors
Shigeoka S, Nakano Y, Kitaoka S.
  Title
Metabolism of hydrogen peroxide in Euglena gracilis Z by L-ascorbic acid peroxidase.
  Journal
Biochem J 186:377-80 (1980)
DOI:10.1042/bj1860377
Reference
3
  Authors
Nakano, Y and Asada, K.
  Title
Purification of ascorbate peroxidase in spinach chloroplasts; its inactivation in ascorbate-depleted medium and reactivation by monodehydroascorbate radical.
  Journal
Plant Cell Physiol 28:131-140 (1987)
Reference
4  [PMID:7703247]
  Authors
Patterson WR, Poulos TL
  Title
Crystal structure of recombinant pea cytosolic ascorbate peroxidase.
  Journal
Biochemistry 34:4331-41 (1995)
DOI:10.1021/bi00013a023
Reference
5  [PMID:15236572]
  Authors
Sharp KH, Moody PC, Brown KA, Raven EL
  Title
Crystal structure of the ascorbate peroxidase-salicylhydroxamic acid complex.
  Journal
Biochemistry 43:8644-51 (2004)
DOI:10.1021/bi049343q
  Sequence
[gmx:553156]
Reference
6  [PMID:16784232]
  Authors
Macdonald IK, Badyal SK, Ghamsari L, Moody PC, Raven EL
  Title
Interaction of ascorbate peroxidase with substrates: a mechanistic and structural analysis.
  Journal
Biochemistry 45:7808-17 (2006)
DOI:10.1021/bi0606849
  Sequence
[gmx:553156]
Other DBs
ExplorEnz - The Enzyme Database: 1.11.1.11
IUBMB Enzyme Nomenclature: 1.11.1.11
ExPASy - ENZYME nomenclature database: 1.11.1.11
BRENDA, the Enzyme Database: 1.11.1.11
CAS: 72906-87-7
LinkDB

KEGG   REACTION: R00644
Entry
R00644                      Reaction                               

Name
L-ascorbate:hydrogen-peroxide oxidoreductase
Definition
Ascorbate + Hydrogen peroxide <=> Dehydroascorbate + 2 H2O
Equation
Reaction class
RC00092  C00072_C05422
Enzyme
Pathway
rn00480  Glutathione metabolism
rn01100  Metabolic pathways
Orthology
K00434  L-ascorbate peroxidase [EC:1.11.1.11]
Other DBs
RHEA: 22999
LinkDB

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