KEGG   ORTHOLOGY: K04283
Entry
K04283                      KO                                     

Name
TRYR
Definition
trypanothione-disulfide reductase [EC:1.8.1.12]
Pathway
ko00480  Glutathione metabolism
ko01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09106 Metabolism of other amino acids
   00480 Glutathione metabolism
    K04283  TRYR; trypanothione-disulfide reductase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.8  Acting on a sulfur group of donors
   1.8.1  With NAD+ or NADP+ as acceptor
    1.8.1.12  trypanothione-disulfide reductase
     K04283  TRYR; trypanothione-disulfide reductase
Other DBs
RN: R03821 R08356 R08357
GO: 0015042
Genes
TBR: Tb10.406.0520
TBG: TbgDal_X12680
TCR: 503555.30
LMA: LMJF_05_0350(TRYR)
LIF: LINJ_05_0350(TRYR)
LDO: LDBPK_050350
LMI: LMXM_05_0350
LBZ: LBRM_05_0350(TRYR)
LPAN: LPMP_050340(TRYR)
AG: CAA78264(TPR) CAA80668
Reference
PMID:3718941
  Authors
Shames SL, Fairlamb AH, Cerami A, Walsh CT
  Title
Purification and characterization of trypanothione reductase from Crithidia fasciculata, a newly discovered member of the family of disulfide-containing flavoprotein reductases.
  Journal
Biochemistry 25:3519-26 (1986)
DOI:10.1021/bi00360a007
  Sequence
Reference
PMID:7607216
  Authors
Cunningham ML, Fairlamb AH
  Title
Trypanothione reductase from Leishmania donovani. Purification, characterisation and inhibition by trivalent antimonials.
  Journal
Eur J Biochem 230:460-8 (1995)
DOI:10.1111/j.1432-1033.1995.0460h.x
  Sequence
LinkDB

KEGG   ENZYME: 1.8.1.12
Entry
EC 1.8.1.12                 Enzyme                                 

Name
trypanothione-disulfide reductase;
trypanothione reductase;
NADPH2:trypanothione oxidoreductase
Class
Oxidoreductases;
Acting on a sulfur group of donors;
With NAD+ or NADP+ as acceptor
Sysname
trypanothione:NADP+ oxidoreductase
Reaction(IUBMB)
trypanothione + NADP+ = trypanothione disulfide + NADPH + H+ [RN:R03821]
Reaction(KEGG)
R03821;
(other) R08356 R08357
Substrate
trypanothione [CPD:C02090];
NADP+ [CPD:C00006]
Product
trypanothione disulfide [CPD:C03170];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
Trypanothione disulfide is the oxidized form of N1,N8-bis(glutathionyl)-spermidine from the insect-parasitic trypanosomatid Crithidia fasciculata. The enzyme from Crithidia fasciculata is a flavoprotein (FAD), whose activity is dependent on a redox-active cystine at the active centre. (cf. EC 1.8.1.7, glutathione-disulfide reductase)
History
EC 1.8.1.12 created 1989 as EC 1.6.4.8, transferred 2002 to EC 1.8.1.12
Pathway
ec00480  Glutathione metabolism
ec01100  Metabolic pathways
Orthology
K04283  trypanothione-disulfide reductase
Genes
TBR: Tb10.406.0520
TBG: TbgDal_X12680
TCR: 503555.30
LMA: LMJF_05_0350(TRYR)
LIF: LINJ_05_0350(TRYR)
LDO: LDBPK_050350
LMI: LMXM_05_0350
LBZ: LBRM_05_0350(TRYR)
LPAN: LPMP_050340(TRYR)
Reference
1  [PMID:3718941]
  Authors
Shames SL, Fairlamb AH, Cerami A, Walsh CT.
  Title
Purification and characterization of trypanothione reductase from Crithidia fasciculata, a newly discovered member of the family of disulfide-containing flavoprotein reductases.
  Journal
Biochemistry 25:3519-26 (1986)
DOI:10.1021/bi00360a007
  Sequence
Reference
2  [PMID:9057833]
  Authors
Marsh IR, Bradley M.
  Title
Substrate specificity of trypanothione reductase.
  Journal
Eur J Biochem 243:690-4 (1997)
DOI:10.1111/j.1432-1033.1997.00690.x
Reference
3  [PMID:7607216]
  Authors
Cunningham ML, Fairlamb AH.
  Title
Trypanothione reductase from Leishmania donovani. Purification, characterisation and inhibition by trivalent antimonials.
  Journal
Eur J Biochem 230:460-8 (1995)
DOI:10.1111/j.1432-1033.1995.0460h.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.8.1.12
IUBMB Enzyme Nomenclature: 1.8.1.12
ExPASy - ENZYME nomenclature database: 1.8.1.12
BRENDA, the Enzyme Database: 1.8.1.12
CAS: 102210-35-5
LinkDB

KEGG   REACTION: R08357
Entry
R08357                      Reaction                               

Name
homotrypanothione:NADP+ oxidoreductase
Definition
Homotrypanothione + NADP+ <=> Homotrypanothione disulfide + NADPH + H+
Equation
Reaction class
RC00001  C00005_C00006
RC01003  C16567_C16568
Enzyme
Pathway
rn00480  Glutathione metabolism
Orthology
K04283  trypanothione-disulfide reductase [EC:1.8.1.12]
LinkDB

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