KEGG   ORTHOLOGY: K05280
Entry
K05280                      KO                                     

Name
CYP75B1
Definition
flavonoid 3'-monooxygenase [EC:1.14.14.82]
Pathway
ko00941  Flavonoid biosynthesis
ko00944  Flavone and flavonol biosynthesis
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00941 Flavonoid biosynthesis
    K05280  CYP75B1; flavonoid 3'-monooxygenase
   00944 Flavone and flavonol biosynthesis
    K05280  CYP75B1; flavonoid 3'-monooxygenase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K05280  CYP75B1; flavonoid 3'-monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.82  flavonoid 3'-monooxygenase
     K05280  CYP75B1; flavonoid 3'-monooxygenase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, plant type
  CYP75 family
   K05280  CYP75B1; flavonoid 3'-monooxygenase
Other DBs
RN: R02442 R03124 R06537 R06538 R08002 R08032
GO: 0016711
Genes
ATH: AT5G07990(TT7)
ALY: 9309404
CRB: 17881114
CSAT: 104708304 104734816 104769003
EUS: EUTSA_v10013289mg
BRP: 103847118
BNA: 106419056 111197716
BOE: 106313667
RSZ: 108814778
THJ: 104801243
CPAP: 110811144
CIT: 102620909
TCC: 18604820
GRA: 105764705
GAB: 108479810
VUN: 114164840
LJA: Lj1g3v2293820.1(Lj1g3v2293820.1) Lj4g3v1708650.1(Lj4g3v1708650.1) Lj4g3v2267360.1(Lj4g3v2267360.1)
ADU: 107471019
AIP: 107623580
RCN: 112178545
PPER: 18777306
PMUM: 103338591
PXB: 103930751
ZJU: 107419096
CSV: 101207160
CMO: 103494036
CMAX: 111499893
CMOS: 111440987
CPEP: 111792334
RCU: 8284440
JCU: 105640611
MESC: 110617654
PEU: 105115867
JRE: 108982908
QSU: 112039462
VVI: 100232999(F3'H) 100263810(F3'H)
SLY: 101266618
SPEN: 107015315
SOT: 102593656
CANN: 107862334
NTA: 107773493(NtF3'H) 107795677(F3'H1)
NTO: 104093246
NAU: 109223789
INI: 109160581(Magenta)
SIND: 105157984
OEU: 111401587
CCAV: 112514430
DCR: 108216209
BVG: 104907876
SOE: 110789886
DOSA: Os10t0317900-01(Os10g0317900) Os10t0320100-01(Os10g0320100)
ATS: 109737789(LOC109737789) 109767455(LOC109767455) 109773560(LOC109773560)
MUS: 103979831
ATR: 18428045
AG: AAD56282(CYP75B2)
 » show all
Reference
  Authors
Schoenbohm C, Martens S, Eder C, Forkmann G, Weisshaar B
  Title
Identification of the Arabidopsis thaliana flavonoid 3'-hydroxylase gene and functional expression of the encoded P450 enzyme.
  Journal
Biol Chem 381:749-53 (2000)
DOI:10.1515/BC.2000.095
  Sequence
[ath:AT5G07990]
LinkDB

KEGG   ENZYME: 1.14.14.82
Entry
EC 1.14.14.82               Enzyme                                 

Name
flavonoid 3'-monooxygenase;
CYP75B1 (gene name);
flavonoid 3'-hydroxylase;
flavonoid 3-hydroxylase (incorrect);
NADPH:flavonoid-3'-hydroxylase (incorrect);
flavonoid 3-monooxygenase (incorrect)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
flavonoid,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (3'-hydroxylating)
Reaction(IUBMB)
a flavonoid + [reduced NADPH---hemoprotein reductase] + O2 = a 3'-hydroxyflavonoid + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R03615]
Reaction(KEGG)
Substrate
flavonoid [CPD:C01579];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
3'-hydroxyflavonoid [CPD:C02790];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein found in plants. Acts on a number of flavonoids, including the flavanone naringenin and the flavone apigenin. Does not act on 4-coumarate or 4-coumaroyl-CoA.
History
EC 1.14.14.82 created 1983 as EC 1.14.13.21, transferred 2018 to EC 1.14.14.82
Pathway
ec00941  Flavonoid biosynthesis
ec00944  Flavone and flavonol biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K05280  flavonoid 3'-monooxygenase
Genes
ATH: AT5G07990(TT7)
ALY: 9309404
CRB: 17881114
CSAT: 104708304 104734816 104769003
EUS: EUTSA_v10013289mg
BRP: 103847118
BNA: 106419056 111197716
BOE: 106313667
RSZ: 108814778
THJ: 104801243
CPAP: 110811144
CIT: 102620909
TCC: 18604820
GRA: 105764705
GAB: 108479810
VUN: 114164840
LJA: Lj1g3v2293820.1(Lj1g3v2293820.1) Lj4g3v1708650.1(Lj4g3v1708650.1) Lj4g3v2267360.1(Lj4g3v2267360.1)
ADU: 107471019
AIP: 107623580
RCN: 112178545
PPER: 18777306
PMUM: 103338591
PXB: 103930751
ZJU: 107419096
CSV: 101207160
CMO: 103494036
CMAX: 111499893
CMOS: 111440987
CPEP: 111792334
RCU: 8284440
JCU: 105640611
MESC: 110617654
PEU: 105115867
JRE: 108982908
QSU: 112039462
VVI: 100232999(F3'H) 100263810(F3'H)
SLY: 101266618
SPEN: 107015315
SOT: 102593656
CANN: 107862334
NTA: 107773493(NtF3'H) 107795677(F3'H1)
NTO: 104093246
NAU: 109223789
INI: 109160581(Magenta)
SIND: 105157984
OEU: 111401587
CCAV: 112514430
DCR: 108216209
BVG: 104907876
SOE: 110789886
DOSA: Os10t0317900-01(Os10g0317900) Os10t0320100-01(Os10g0320100)
ATS: 109737789(LOC109737789) 109767455(LOC109767455) 109773560(LOC109773560)
MUS: 103979831
ATR: 18428045
 » show all
Reference
1
  Authors
Forkmann, G., Heller, W. and Grisebach, H.
  Title
Anthocyanin biosynthesis in flowers of Matthiola incana flavanone 3- and flavonoid 3'-hydroxylases.
  Journal
Z Naturforsch C: Biosci 35:691-695 (1980)
Reference
2  [PMID:10504566]
  Authors
Brugliera F, Barri-Rewell G, Holton TA, Mason JG
  Title
Isolation and characterization of a flavonoid 3'-hydroxylase cDNA clone corresponding to the Ht1 locus of Petunia hybrida.
  Journal
Plant J 19:441-51 (1999)
DOI:10.1046/j.1365-313X.1999.00539.x
  Sequence
Reference
3  [PMID:11030432]
  Authors
Schoenbohm C, Martens S, Eder C, Forkmann G, Weisshaar B
  Title
Identification of the Arabidopsis thaliana flavonoid 3'-hydroxylase gene and functional expression of the encoded P450 enzyme.
  Journal
Biol Chem 381:749-53 (2000)
DOI:10.1515/BC.2000.095
  Sequence
[ath:AT5G07990]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.82
IUBMB Enzyme Nomenclature: 1.14.14.82
ExPASy - ENZYME nomenclature database: 1.14.14.82
BRENDA, the Enzyme Database: 1.14.14.82
CAS: 75991-44-5
LinkDB

KEGG   REACTION: R06537
Entry
R06537                      Reaction                               

Name
apigenin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (3'-hydroxylating)
Definition
Apigenin + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> Luteolin + [Oxidized NADPH---hemoprotein reductase] + H2O
Equation
Comment
flavonoid 3'-hydroxylase
Reaction class
RC00001  C00005_C00006
RC00046  C01477_C01514
Enzyme
Pathway
rn00941  Flavonoid biosynthesis
rn00944  Flavone and flavonol biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Orthology
K05280  flavonoid 3'-monooxygenase [EC:1.14.14.82]
K13083  flavonoid 3',5'-hydroxylase [EC:1.14.14.81]
LinkDB

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