KEGG   ORTHOLOGY: K05297
Entry
K05297                      KO                                     

Name
rubB, alkT
Definition
rubredoxin---NAD+ reductase [EC:1.18.1.1]
Pathway
ko00071  Fatty acid degradation
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09103 Lipid metabolism
   00071 Fatty acid degradation
    K05297  rubB, alkT; rubredoxin---NAD+ reductase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.18  Acting on iron-sulfur proteins as donors
   1.18.1  With NAD+ or NADP+ as acceptor
    1.18.1.1  rubredoxin---NAD+ reductase
     K05297  rubB, alkT; rubredoxin---NAD+ reductase
Other DBs
RN: R02000
COG: COG1251
GO: 0015044
Genes
XBA: C7S18_09175
VAG: N646_3145
VEX: VEA_001294
VDB: AL552_01515
VNA: PN96_15080
VEJ: VEJY3_23206
VNL: D3H41_24160
SKS: FCN78_06135 FCN78_12245
PAE: PA5349
PAEV: N297_5529(alkT)
PAEI: N296_5529(alkT)
PAU: PA14_70620(rubB)
PNC: NCGM2_6116(rubB)
PAEB: NCGM1900_6194(rubB)
PAEP: PA1S_28900
PAEM: U769_29400
PAEL: T223_29360
PAEG: AI22_05090
PAEC: M802_5527(alkT)
PAEO: M801_5394(alkT)
PMY: Pmen_4402
PMK: MDS_4734
PPSE: BN5_4231(rubB)
PCQ: PcP3B5_60770(alkT)
PPU: PP_5314(alkT) PP_5371
PPF: Pput_5223
PPT: PPS_5163
PPB: PPUBIRD1_5107(rubB)
PPI: YSA_04864
PPX: T1E_3940(rubB) T1E_3999
PPUH: B479_26305
PPUT: L483_31710
PPUN: PP4_53750(rubB)
PPUD: DW66_5733
PMON: X969_25520
PMOT: X970_25155
PST: PSPTO_5473(rubB)
PSB: Psyr_5028
PSYR: N018_25385
PSP: PSPPH_5110(rubB)
PVD: CFBP1590__5340(alkT)
PFL: PFL_6103(rubB)
PPRC: PFLCHA0_c60620(alkT)
PPRO: PPC_6056
PFO: Pfl01_5601(norW)
PFS: PFLU_6033
PFE: PSF113_0127(rubB)
PFC: PflA506_5316(rubB)
PFB: VO64_2925
PFW: PF1751_v1c54440(rubB)
PEN: PSEEN5459(rubB)
PSA: PST_0438(rubB)
PSZ: PSTAB_0469(rubB)
PSR: PSTAA_0491(rubB)
PSTT: CH92_01830
PKC: PKB_5680(alkT)
PSEM: TO66_31050
PSEC: CCOS191_5209(alkT)
PSOS: POS17_6064
PANR: A7J50_5768
PSET: THL1_5641
PSIL: PMA3_29615
PALI: A3K91_2007
ACB: A1S_0994
ABM: ABSDF2404(rubB)
ABY: ABAYE1067 ABAYE2799(rubB)
ABB: ABBFA_02593(rubB)
ABAD: ABD1_09470(rubB)
ABAZ: P795_12740
ABAU: IX87_00560
ABAA: IX88_06985
ACC: BDGL_000275(rubB)
ACI: ACIAD1065(rubB)
ASJ: AsACE_CH00799(rubB)
AGU: AS4_32640(rubB)
AUG: URS_2062
MCT: MCR_0041
MCS: DR90_38
MCAT: MC25239_00086(norW)
MCUN: NCTC10297_00560(norW)
SDN: Sden_3466
PART: PARC_a1586
MAQ: Maqu_3591
MHC: MARHY3494(rubB)
MAD: HP15_3349
MBS: MRBBS_3587(alkT)
MARJ: MARI_30590(alkT_2)
SAGA: M5M_15220
MICC: AUP74_00095(rubB_1) AUP74_02936(rubB_2)
MICT: FIU95_16400(rubB)
CBU: CBU_0276
CBD: CBUD_1816
CBG: CbuG_1731
CBC: CbuK_0471
TCX: Tcr_0037
TIG: THII_3133
AEH: Mlg_0757
TGR: Tgr7_0681
TKM: TK90_0972
TVR: TVD_08070
HCH: HCH_00777
CSA: Csal_3250
HEL: HELO_4414(rubB)
HAM: HALO0041
HCO: LOKO_02284(alkT)
HOL: HORIV_72020(rubB)
HSR: HSBAA_65910(alkT)
ABO: ABO_0162(rubB)
ADI: B5T_02839 B5T_04348(rubB)
APAC: S7S_17715
AXE: P40_21175
TOL: TOL_0163
OAI: OLEAN_C38720(rubB)
OCE: GU3_07995
SALN: SALB1_1968
LHK: LHK_03088
BCON: NL30_16215
PNU: Pnuc_1378
PLG: NCTC10937_05175(alkT)
CABA: SBC2_52550(norW)
AMIM: MIM_c34090
RFR: Rfer_0092
HYB: Q5W_21975
MPT: Mpe_A3669
HAR: HEAR0644
MMS: mma_0610(rubB)
JAG: GJA_3183(alkT) GJA_559(rubB)
CFU: CFU_0845(rubB)
CARE: LT85_0937
CPRA: CPter91_0986(alkT)
NEU: NE1420
NET: Neut_1534
DOE: DENOEST_0186(rubB)
TBD: Tbd_2315
MFA: Mfla_2173
GCA: Galf_2385
SPLB: SFPGR_33950(alkT)
SLAC: SKTS_14210(rubB)
EBA: ebA2965(norW) ebA6036
AOA: dqs_2624
AZA: AZKH_0051(norW) AZKH_2404(rubB)
BPRC: D521_1162
MDI: METDI1722
MEX: Mext_1149
MCH: Mchl_1308
MPO: Mpop_4666
MOR: MOC_5231
META: Y590_04930
SPKC: KC8_06485
SECH: B18_09050
SPHT: K426_24049
BLAS: BSY18_2508
ASZ: ASN_3251
MAG: amb3427
MAGX: XM1_4310(rubB)
MAGN: WV31_00580
MAGQ: MGMAQ_0418(rubB)
MGM: Mmc1_3270
 » show all
Reference
  Authors
Smits TH, Witholt B, van Beilen JB
  Title
Functional characterization of genes involved in alkane oxidation by Pseudomonas aeruginosa.
  Journal
Antonie Van Leeuwenhoek 84:193-200 (2003)
DOI:10.1023/A:1026000622765
  Sequence
[pae:PA5349]
Reference
  Authors
Miri M, Bambai B, Tabandeh F, Sadeghizadeh M, Kamali N
  Title
Production of a recombinant alkane hydroxylase (AlkB2) from Alcanivorax borkumensis.
  Journal
Biotechnol Lett 32:497-502 (2010)
DOI:10.1007/s10529-009-0177-0
  Sequence
[abo:ABO_0162]
Reference
  Authors
Geissdorfer W, Kok RG, Ratajczak A, Hellingwerf KJ, Hillen W
  Title
The genes rubA and rubB for alkane degradation in Acinetobacter sp. strain ADP1 are in an operon with estB, encoding an esterase, and oxyR.
  Journal
J Bacteriol 181:4292-8 (1999)
DOI:10.1128/JB.181.14.4292-4298.1999
  Sequence
[aci:ACIAD1065]
LinkDB

KEGG   ORTHOLOGY: K21738
Entry
K21738                      KO                                     

Name
alkT
Definition
rubredoxin---NAD+ reductase [EC:1.18.1.1]
Pathway
ko00071  Fatty acid degradation
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09103 Lipid metabolism
   00071 Fatty acid degradation
    K21738  alkT; rubredoxin---NAD+ reductase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.18  Acting on iron-sulfur proteins as donors
   1.18.1  With NAD+ or NADP+ as acceptor
    1.18.1.1  rubredoxin---NAD+ reductase
     K21738  alkT; rubredoxin---NAD+ reductase
Other DBs
RN: R02000
COG: COG0446
GO: 0015044
Genes
PMY: Pmen_0430
MAQ: Maqu_0442
MHC: MARHY2849(alkT)
MSR: AU15_22050
MARI: ACP86_20395
MLQ: ASQ50_08875
MARJ: MARI_17880(alkT_1)
SPSE: SULPSESMR1_04189(alkT)
MON: G8E03_03690
SPHY: CHN51_00400
AG: CAB54063(alkT)
 » show all
Reference
PMID:2319593
  Authors
Eggink G, Engel H, Vriend G, Terpstra P, Witholt B
  Title
Rubredoxin reductase of Pseudomonas oleovorans. Structural relationship to other flavoprotein oxidoreductases based on one NAD and two FAD fingerprints.
  Journal
J Mol Biol 212:135-42 (1990)
DOI:10.1016/0022-2836(90)90310-I
  Sequence
[ag:CAB54063] [pmy:Pmen_0430]
Reference
PMID:4335861
  Title
Enzymatic -oxidation. VI. Isolation of homogeneous reduced diphosphopyridine nucleotide-rubredoxin reductase.
  Journal
J Biol Chem 247:2109-16 (1972)
LinkDB

KEGG   ORTHOLOGY: K22567
Entry
K22567                      KO                                     

Name
nroR
Definition
rubredoxin---NAD+ reductase [EC:1.18.1.1]
Pathway
ko00071  Fatty acid degradation
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09103 Lipid metabolism
   00071 Fatty acid degradation
    K22567  nroR; rubredoxin---NAD+ reductase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.18  Acting on iron-sulfur proteins as donors
   1.18.1  With NAD+ or NADP+ as acceptor
    1.18.1.1  rubredoxin---NAD+ reductase
     K22567  nroR; rubredoxin---NAD+ reductase
Other DBs
RN: R02000
COG: COG1251
GO: 0015044
Genes
CAC: CA_C2448
CAE: SMB_G2483
CAY: CEA_G2462
Reference
  Authors
Guedon E, Petitdemange H
  Title
Identification of the gene encoding NADH-rubredoxin oxidoreductase in Clostridium acetobutylicum.
  Journal
Biochem Biophys Res Commun 285:496-502 (2001)
DOI:10.1006/bbrc.2001.5196
  Sequence
[cac:CA_C2448]
Reference
  Authors
Riebe O, Fischer RJ, Wampler DA, Kurtz DM Jr, Bahl H
  Title
Pathway for H2O2 and O2 detoxification in Clostridium acetobutylicum.
  Journal
Microbiology 155:16-24 (2009)
DOI:10.1099/mic.0.022756-0
  Sequence
[cac:CA_C2448]
LinkDB

KEGG   ENZYME: 1.18.1.1
Entry
EC 1.18.1.1                 Enzyme                                 

Name
rubredoxin---NAD+ reductase;
rubredoxin reductase;
rubredoxin-nicotinamide adenine dinucleotide reductase;
dihydronicotinamide adenine dinucleotide-rubredoxin reductase;
reduced nicotinamide adenine dinucleotide-rubredoxin reductase;
NADH-rubredoxin reductase;
rubredoxin-NAD reductase;
NADH: rubredoxin oxidoreductase;
DPNH-rubredoxin reductase;
NADH-rubredoxin oxidoreductase
Class
Oxidoreductases;
Acting on iron-sulfur proteins as donors;
With NAD+ or NADP+ as acceptor
Sysname
rubredoxin:NAD+ oxidoreductase
Reaction(IUBMB)
2 reduced rubredoxin + NAD+ + H+ = 2 oxidized rubredoxin + NADH [RN:R02000]
Reaction(KEGG)
R02000
Substrate
reduced rubredoxin [CPD:C00340];
NAD+ [CPD:C00003];
H+ [CPD:C00080]
Product
oxidized rubredoxin [CPD:C00435];
NADH [CPD:C00004]
Comment
Requires FAD. The enzyme from Clostridium acetobutylicum reduces rubredoxin, ferricyanide and dichlorophenolindophenol, but not ferredoxin or flavodoxin. The reaction does not occur when NADPH is substituted for NADH. Contains iron at the redox centre.
History
EC 1.18.1.1 created 1972 as EC 1.6.7.2, transferred 1978 to EC 1.18.1.1, modified 2001
Pathway
ec00071  Fatty acid degradation
Orthology
K05297  rubredoxin---NAD+ reductase
K21738  rubredoxin---NAD+ reductase
K22567  rubredoxin---NAD+ reductase
Genes
XBA: C7S18_09175
VAG: N646_3145
VEX: VEA_001294
VDB: AL552_01515
VNA: PN96_15080
VEJ: VEJY3_23206
VNL: D3H41_24160
SKS: FCN78_06135 FCN78_12245
PAE: PA5349
PAEV: N297_5529(alkT)
PAEI: N296_5529(alkT)
PAU: PA14_70620(rubB)
PNC: NCGM2_6116(rubB)
PAEB: NCGM1900_6194(rubB)
PAEP: PA1S_28900
PAEM: U769_29400
PAEL: T223_29360
PAEG: AI22_05090
PAEC: M802_5527(alkT)
PAEO: M801_5394(alkT)
PMK: MDS_4734
PPSE: BN5_4231(rubB)
PCQ: PcP3B5_60770(alkT)
PPU: PP_5314(alkT) PP_5371
PPF: Pput_5223
PPT: PPS_5163
PPB: PPUBIRD1_5107(rubB)
PPI: YSA_04864
PPX: T1E_3940(rubB) T1E_3999
PPUH: B479_26305
PPUT: L483_31710
PPUN: PP4_53750(rubB)
PPUD: DW66_5733
PMON: X969_25520
PMOT: X970_25155
PST: PSPTO_5473(rubB)
PSB: Psyr_5028
PSYR: N018_25385
PSP: PSPPH_5110(rubB)
PVD: CFBP1590__5340(alkT)
PFL: PFL_6103(rubB)
PPRC: PFLCHA0_c60620(alkT)
PPRO: PPC_6056
PFO: Pfl01_5601(norW)
PFS: PFLU_6033
PFE: PSF113_0127(rubB)
PFC: PflA506_5316(rubB)
PFB: VO64_2925
PFW: PF1751_v1c54440(rubB)
PEN: PSEEN5459(rubB)
PSA: PST_0438(rubB)
PSZ: PSTAB_0469(rubB)
PSR: PSTAA_0491(rubB)
PSTT: CH92_01830
PKC: PKB_5680(alkT)
PSEM: TO66_31050
PSEC: CCOS191_5209(alkT)
PSOS: POS17_6064
PANR: A7J50_5768
PSET: THL1_5641
PSIL: PMA3_29615
PALI: A3K91_2007
ACB: A1S_0994
ABM: ABSDF2404(rubB)
ABY: ABAYE1067 ABAYE2799(rubB)
ABB: ABBFA_02593(rubB)
ABAD: ABD1_09470(rubB)
ABAZ: P795_12740
ABAU: IX87_00560
ABAA: IX88_06985
ACC: BDGL_000275(rubB)
ACI: ACIAD1065(rubB)
ASJ: AsACE_CH00799(rubB)
AGU: AS4_32640(rubB)
AUG: URS_2062
MCT: MCR_0041
MCS: DR90_38
MCAT: MC25239_00086(norW)
MCUN: NCTC10297_00560(norW)
SDN: Sden_3466
PART: PARC_a1586
MHC: MARHY2849(alkT) MARHY3494(rubB)
MAD: HP15_3349
MBS: MRBBS_3587(alkT)
MARJ: MARI_17880(alkT_1) MARI_30590(alkT_2)
SAGA: M5M_15220
MICC: AUP74_00095(rubB_1) AUP74_02936(rubB_2)
MICT: FIU95_16400(rubB)
CBU: CBU_0276
CBD: CBUD_1816
CBG: CbuG_1731
CBC: CbuK_0471
TCX: Tcr_0037
TIG: THII_3133
AEH: Mlg_0757
TGR: Tgr7_0681
TKM: TK90_0972
TVR: TVD_08070
HCH: HCH_00777
CSA: Csal_3250
HEL: HELO_4414(rubB)
HAM: HALO0041
HCO: LOKO_02284(alkT)
HOL: HORIV_72020(rubB)
HSR: HSBAA_65910(alkT)
ABO: ABO_0162(rubB)
ADI: B5T_02839 B5T_04348(rubB)
APAC: S7S_17715
AXE: P40_21175
TOL: TOL_0163
OAI: OLEAN_C38720(rubB)
OCE: GU3_07995
SALN: SALB1_1968
LHK: LHK_03088
BCON: NL30_16215
PNU: Pnuc_1378
PLG: NCTC10937_05175(alkT)
CABA: SBC2_52550(norW)
AMIM: MIM_c34090
RFR: Rfer_0092
HYB: Q5W_21975
MPT: Mpe_A3669
HAR: HEAR0644
MMS: mma_0610(rubB)
JAG: GJA_3183(alkT) GJA_559(rubB)
CFU: CFU_0845(rubB)
CARE: LT85_0937
CPRA: CPter91_0986(alkT)
NEU: NE1420
NET: Neut_1534
DOE: DENOEST_0186(rubB)
TBD: Tbd_2315
MFA: Mfla_2173
GCA: Galf_2385
SPLB: SFPGR_33950(alkT)
SLAC: SKTS_14210(rubB)
EBA: ebA2965(norW) ebA6036
AOA: dqs_2624
AZA: AZKH_0051(norW) AZKH_2404(rubB)
BPRC: D521_1162
MDI: METDI1722
MEX: Mext_1149
MCH: Mchl_1308
MPO: Mpop_4666
MOR: MOC_5231
META: Y590_04930
SPSE: SULPSESMR1_04189(alkT)
SPKC: KC8_06485
SECH: B18_09050
SPHT: K426_24049
BLAS: BSY18_2508
ASZ: ASN_3251
MAG: amb3427
MAGX: XM1_4310(rubB)
MAGN: WV31_00580
MAGQ: MGMAQ_0418(rubB)
MGM: Mmc1_3270
CAC: CA_C2448
CAE: SMB_G2483
CAY: CEA_G2462
 » show all
Reference
1  [PMID:4294330]
  Authors
Peterson JA, Kusunose M, Kusunose E, Coon MJ.
  Title
Enzymatic omega-oxidation. II. Function of rubredoxin as the electron carrier in omega-hydroxylation.
  Journal
J Biol Chem 242:4334-40 (1967)
Reference
2  [PMID:4335861]
  Authors
.
  Title
Enzymatic  -oxidation. VI. Isolation of homogeneous reduced diphosphopyridine nucleotide-rubredoxin reductase.
  Journal
J Biol Chem 247:2109-16 (1972)
Reference
3  [PMID:4403503]
  Authors
Ueda T, Coon MJ.
  Title
Enzymatic   oxidation. VII. Reduced diphosphopyridine nucleotide-rubredoxin reductase: properties and function as an electron carrier in   hydroxylation.
  Journal
J Biol Chem 247:5010-6 (1972)
Reference
4  [PMID:526302]
  Authors
Petitdemange H, Marczak R, Blusson H, Gay R.
  Title
Isolation and properties of reduced nicotinamide adenine dinucleotiderubredoxin oxidoreductase of Clostridium acetobutylicum.
  Journal
Biochem Biophys Res Commun 91:1258-65 (1979)
DOI:10.1016/0006-291X(79)91202-6
Other DBs
ExplorEnz - The Enzyme Database: 1.18.1.1
IUBMB Enzyme Nomenclature: 1.18.1.1
ExPASy - ENZYME nomenclature database: 1.18.1.1
BRENDA, the Enzyme Database: 1.18.1.1
CAS: 9032-27-3
LinkDB

KEGG   REACTION: R02000
Entry
R02000                      Reaction                               

Name
rubredoxin:NAD+ oxidoreductase
Definition
2 Reduced rubredoxin + NAD+ + H+ <=> 2 Oxidized rubredoxin + NADH
Equation
Reaction class
RC00001  C00003_C00004
Enzyme
1.18.1.1        1.18.1.3        1.18.1.4
Pathway
rn00071  Fatty acid degradation
Orthology
K00529  3-phenylpropionate/trans-cinnamate dioxygenase ferredoxin reductase component [EC:1.18.1.3]
K05297  rubredoxin---NAD+ reductase [EC:1.18.1.1]
K21738  rubredoxin---NAD+ reductase [EC:1.18.1.1]
K22567  rubredoxin---NAD+ reductase [EC:1.18.1.1]
Other DBs
RHEA: 18600
LinkDB

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