KEGG   ORTHOLOGY: K11778
Entry
K11778                      KO                                     

Name
DHDDS, RER2, SRT1
Definition
ditrans,polycis-polyprenyl diphosphate synthase [EC:2.5.1.87]
Pathway
ko00900  Terpenoid backbone biosynthesis
ko01110  Biosynthesis of secondary metabolites
Disease
H00527  Retinitis pigmentosa
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00900 Terpenoid backbone biosynthesis
    K11778  DHDDS, RER2, SRT1; ditrans,polycis-polyprenyl diphosphate synthase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01006 Prenyltransferases
    K11778  DHDDS, RER2, SRT1; ditrans,polycis-polyprenyl diphosphate synthase
Enzymes [BR:ko01000]
 2. Transferases
  2.5  Transferring alkyl or aryl groups, other than methyl groups
   2.5.1  Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
    2.5.1.87  ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific]
     K11778  DHDDS, RER2, SRT1; ditrans,polycis-polyprenyl diphosphate synthase
Prenyltransferases [BR:ko01006]
 Terpene biosynthesis
  Prenyl diphosphate synthase
   K11778  DHDDS, RER2, SRT1; ditrans,polycis-polyprenyl diphosphate synthase
Other DBs
RN: R05556
COG: COG0020
GO: 0045547
Genes
HSA: 79947(DHDDS)
PTR: 456662(DHDDS)
PPS: 100972313(DHDDS)
GGO: 101125977(DHDDS)
PON: 100171948(DHDDS)
NLE: 100603801(DHDDS)
MCC: 714635(DHDDS)
MCF: 101926129(DHDDS)
CSAB: 103225956(DHDDS)
RRO: 104675752(DHDDS)
RBB: 108518187(DHDDS)
CJC: 100405741(DHDDS)
SBQ: 101046544(DHDDS)
MMU: 67422(Dhdds)
MCAL: 110293104(Dhdds)
MPAH: 110323123(Dhdds)
RNO: 298541(Dhdds)
MUN: 110552313(Dhdds)
CGE: 100767025(Dhdds)
NGI: 103737115(Dhdds)
HGL: 101711794(Dhdds)
CCAN: 109683062(Dhdds)
OCU: 100358963(DHDDS)
TUP: 102482449(DHDDS)
CFA: 612157(DHDDS)
VVP: 112931674(DHDDS)
AML: 100463534(DHDDS)
UMR: 103666770(DHDDS)
UAH: 113268456(DHDDS)
ORO: 101375137(DHDDS)
ELK: 111154780
FCA: 101085905(DHDDS)
PTG: 102966133(DHDDS)
PPAD: 109256774(DHDDS)
AJU: 106977611(DHDDS)
BTA: 523264(DHDDS)
BOM: 102271246(DHDDS)
BIU: 109573388(DHDDS)
BBUB: 102394454(DHDDS)
CHX: 102169144(DHDDS)
OAS: 101110654(DHDDS)
SSC: 100524683(DHDDS)
CFR: 102518547(DHDDS)
CDK: 105100689(DHDDS)
BACU: 102998703(DHDDS)
LVE: 103088197(DHDDS)
OOR: 101277244(DHDDS)
DLE: 111163358(DHDDS)
PCAD: 102994453(DHDDS)
ECB: 100057242(DHDDS)
EPZ: 103550540(DHDDS)
EAI: 106839917(DHDDS)
MYB: 102238802(DHDDS)
MYD: 102758520(DHDDS)
MNA: 107539928(DHDDS)
HAI: 109386757(DHDDS)
DRO: 112299245(DHDDS)
PALE: 102891420(DHDDS)
RAY: 107503570(DHDDS)
MJV: 108399008
LAV: 100675778(DHDDS)
TMU: 101345368
MDO: 100012356(DHDDS)
SHR: 100922631(DHDDS)
PCW: 110206127(DHDDS)
OAA: 100083017(DHDDS)
MGP: 100541233(DHDDS)
CJO: 107324014(DHDDS)
NMEL: 110387387(DHDDS)
APLA: 101804205(DHDDS)
ACYG: 106047921(DHDDS)
TGU: 100230447(DHDDS)
LSR: 110472863(DHDDS)
SCAN: 103823525(DHDDS)
GFR: 102038851(DHDDS)
FAB: 101816671(DHDDS)
PHI: 102105638(DHDDS)
PMAJ: 107214256(DHDDS)
CCAE: 111939089(DHDDS)
CCW: 104694634(DHDDS)
ETL: 114068054(DHDDS)
FPG: 101919832(DHDDS)
FCH: 102049798(DHDDS)
CLV: 102098335(DHDDS)
EGZ: 104134887(DHDDS)
NNI: 104017719(DHDDS)
ACUN: 113488396(DHDDS)
PADL: 103925863(DHDDS)
AAM: 106485593(DHDDS)
ASN: 102374803(DHDDS)
AMJ: 102557872(DHDDS)
PSS: 102444519(DHDDS)
CMY: 102941423(DHDDS)
CPIC: 101949942(DHDDS)
ACS: 100552810(dhdds)
PVT: 110086158(DHDDS)
PBI: 103067643(DHDDS)
PMUR: 107293795(DHDDS)
TSR: 106547071(DHDDS)
PMUA: 114601527(DHDDS)
GJA: 107110426(DHDDS)
XLA: 379162(dhdds.S) 398626(dhdds.L)
XTR: 100135207(dhdds)
NPR: 108800534(DHDDS)
DRE: 406468(dhdds)
SRX: 107707633(dhdds) 107719311
SGH: 107552517(dhdds) 107564097
IPU: 108256361(dhdds)
PHYP: 113533089(dhdds)
AMEX: 103040074(dhdds)
EEE: 113583554(dhdds)
TRU: 101067613(dhdds)
LCO: 104924709(dhdds)
NCC: 104959484
MZE: 101465746(dhdds)
ONL: 100705873(dhdds)
OLA: 101156768(dhdds)
XMA: 102232893(dhdds)
XCO: 114141550(dhdds)
PRET: 103477772(dhdds)
CVG: 107082951(dhdds)
NFU: 107379325(dhdds)
KMR: 108240318(dhdds)
ALIM: 106532228(dhdds)
AOCE: 111578588(dhdds)
CSEM: 103388088(dhdds)
POV: 109632991(dhdds)
LCF: 108899452(dhdds)
SDU: 111235772(dhdds)
SLAL: 111651765(dhdds)
HCQ: 109515435(dhdds)
BPEC: 110174299(dhdds)
MALB: 109954705(dhdds)
ELS: 105028368(dhdds)
SFM: 108919101(dhdds)
PKI: 111844563(dhdds)
LCM: 102361662(DHDDS)
CMK: 103179564(dhdds)
RTP: 109937745(dhdds)
BFO: 118415667
CIN: 100179261
SPU: 591381
APLC: 110974411
SKO: 100375715
DME: Dmel_CG10778(CG10778)
DER: 6551609
DSE: 6620258
DSI: Dsimw501_GD16139(Dsim_GD16139)
DAN: 6503683
DSR: 110184809
DPE: 6600428
DMN: 108153958
DWI: 6644774
DAZ: 108618390
DNV: 108655954
DHE: 111602838
DVI: 6631360
MDE: 101899706
LCQ: 111674513
AAG: 5567376
AALB: 109426183
AME: 551358
BIM: 100747096
BTER: 100652219
CCAL: 108626907
OBB: 114875046
ACEP: 105619665
PBAR: 105429911
DQU: 106743998
CFO: 105258120
LHU: 105679126
PGC: 109860006
OBO: 105276969
PCF: 106793556
NVI: 100122811
CSOL: 105363743
MDL: 103569336
TCA: 661474
DPA: 109536787
ATD: 109603363
BMOR: 732991(Dhdds)
BMAN: 114241813
PMAC: 106715662
PRAP: 110996230
HAW: 110377390
TNL: 113507305
PXY: 105386183
DNX: 107163442
BTAB: 109042179
CLEC: 106661118
ZNE: 110838943
PVM: 113800507
TUT: 107362638
DPTE: 113790149
CSCU: 111614013
PTEP: 107448745
CEL: CELE_T01G1.4(T01G1.4)
BMY: Bm1_28770
TSP: Tsp_03385
PCAN: 112565857
CRG: 105317193
MYI: 110456448
OBI: 106875357
LAK: 106155728
SHX: MS3_05662
EGL: EGR_09410
NVE: 5510461
EPA: 110251744
ADF: 107338445
AMIL: 114958441
PDAM: 113671882
SPIS: 111333576
DGT: 114536433
HMG: 101238202
AQU: 100633152
ATH: AT1G11755(LEW1) AT2G17570(cPT1) AT2G23400 AT2G23410(CPT) AT5G58770(cPT4) AT5G58780(cPT5) AT5G58782(cPT6) AT5G58784 AT5G60500(cPT8) AT5G60510(cPT9)
LJA: Lj0g3v0314369.1(Lj0g3v0314369.1) Lj0g3v0326269.1(Lj0g3v0326269.1) Lj0g3v0326269.3(Lj0g3v0326269.3) Lj1g3v0131170.1(Lj1g3v0131170.1)
SLY: 101244138(CPT4) 101250227(SlCPTBP) 101253666(CPT3) 101256886(CPT7) 101267417 104644439
DOSA: Os01t0857200-01(Os01g0857200) Os02t0197700-01(Os02g0197700) Os03t0197000-01(Os03g0197000) Os06t0167400-01(Os06g0167400) Os07t0607700-01(Os07g0607700)
ATS: 109742971(LOC109742971) 109757670(LOC109757670) 109757672(LOC109757672) 109757676(LOC109757676) 109759695(LOC109759695) 109762622 109769504 109772847(LOC109772847)
APRO: F751_3077
SCE: YBR002C(RER2) YMR101C(SRT1)
KMX: KLMA_30092(SRT1) KLMA_60115(RER2)
NCS: NCAS_0E01380(NCAS0E01380) NCAS_0F01440(NCAS0F01440)
NDI: NDAI_0G01550(NDAI0G01550) NDAI_0H02320(NDAI0H02320)
TPF: TPHA_0A04470(TPHA0A04470) TPHA_0G02950(TPHA0G02950)
TBL: TBLA_0B08960(TBLA0B08960) TBLA_0F00550(TBLA0F00550)
TDL: TDEL_0A02280(TDEL0A02280) TDEL_0D04190(TDEL0D04190)
KAF: KAFR_0A00610(KAFR0A00610) KAFR_0F02540(KAFR0F02540)
SLB: AWJ20_395(RER2)
NCR: NCU03460
NTE: NEUTE1DRAFT84674(NEUTE1DRAFT_84674)
MGR: MGG_12110
SSCK: SPSK_09391
MAW: MAC_02906
MAJ: MAA_01682
BFU: BCIN_05g03890(Bcrer2)
MBE: MBM_04118
ANI: AN5600.2
ANG: ANI_1_1896184(An04g05250)
PTE: PTT_19071
CNE: CND03430
CNB: CNBD2910
TASA: A1Q1_02236
ABP: AGABI1DRAFT110974(AGABI1DRAFT_110974)
ABV: AGABI2DRAFT190174(AGABI2DRAFT_190174)
MGL: MGL_1818
MRT: MRET_1995
DFA: DFA_08567
PCB: PCHAS_093380(PC000683.01.0)
TAN: TA04455
TPV: TP03_0421
BBO: BBOV_IV002310(21.m02978)
 » show all
Reference
  Authors
Jones J, Viswanathan K, Krag SS, Betenbaugh MJ
  Title
Polyprenyl lipid synthesis in mammalian cells expressing human cis-prenyl transferase.
  Journal
Biochem Biophys Res Commun 331:379-83 (2005)
DOI:10.1016/j.bbrc.2005.03.181
Reference
PMID:9858571
  Authors
Sato M, Sato K, Nishikawa S, Hirata A, Kato J, Nakano A
  Title
The yeast RER2 gene, identified by endoplasmic reticulum protein localization mutations, encodes cis-prenyltransferase, a key enzyme in dolichol synthesis.
  Journal
Mol Cell Biol 19:471-83 (1999)
DOI:10.1128/MCB.19.1.471
  Sequence
Reference
  Authors
Oh SK, Han KH, Ryu SB, Kang H
  Title
Molecular cloning, expression, and functional analysis of a cis-prenyltransferase from Arabidopsis thaliana. Implications in rubber biosynthesis.
  Journal
J Biol Chem 275:18482-8 (2000)
DOI:10.1074/jbc.M002000200
  Sequence
[ath:AT2G23410]
Reference
  Authors
Cunillera N, Arro M, Fores O, Manzano D, Ferrer A
  Title
Characterization of dehydrodolichyl diphosphate synthase of Arabidopsis thaliana, a key enzyme in dolichol biosynthesis.
  Journal
FEBS Lett 477:170-4 (2000)
DOI:10.1016/S0014-5793(00)01798-1
  Sequence
[ath:AT2G23410]
Reference
  Authors
Zhang H, Ohyama K, Boudet J, Chen Z, Yang J, Zhang M, Muranaka T, Maurel C, Zhu JK, Gong Z
  Title
Dolichol biosynthesis and its effects on the unfolded protein response and abiotic stress resistance in Arabidopsis.
  Journal
Plant Cell 20:1879-98 (2008)
DOI:10.1105/tpc.108.061150
LinkDB

KEGG   ORTHOLOGY: K19177
Entry
K19177                      KO                                     

Name
NUS1
Definition
dehydrodolichyl diphosphate syntase complex subunit NUS1 [EC:2.5.1.87]
Pathway
ko00900  Terpenoid backbone biosynthesis
ko01110  Biosynthesis of secondary metabolites
Disease
H00118  Congenital disorders of glycosylation type I
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00900 Terpenoid backbone biosynthesis
    K19177  NUS1; dehydrodolichyl diphosphate syntase complex subunit NUS1
Enzymes [BR:ko01000]
 2. Transferases
  2.5  Transferring alkyl or aryl groups, other than methyl groups
   2.5.1  Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
    2.5.1.87  ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific]
     K19177  NUS1; dehydrodolichyl diphosphate syntase complex subunit NUS1
Other DBs
RN: R05556
COG: COG0020
GO: 0045547
Genes
HSA: 116150(NUS1)
PTR: 462965(NUS1) 741304
PPS: 100982942(NUS1) 100988367
GGO: 101137725(NUS1)
PON: 100460016(NUS1)
NLE: 100602333(NUS1)
MCC: 715015(NUS1)
MCF: 102126512(NUS1)
CSAB: 103240491(NUS1)
RRO: 104654169(NUS1)
RBB: 108523008(NUS1)
CJC: 100412462(NUS1) 100415318
SBQ: 101028238(NUS1)
MMU: 52014(Nus1)
MCAL: 110303806(Nus1)
MPAH: 110326546(Nus1)
RNO: 294400(Nus1)
MUN: 110541276(Nus1)
CGE: 100761045 100761477(Nus1)
NGI: 103736296(Nus1)
HGL: 101700845(Nus1)
CCAN: 109693829(Nus1)
OCU: 100339242(NUS1)
TUP: 102478223(NUS1)
CFA: 484102(NUS1)
VVP: 112922764(NUS1)
AML: 100466277(NUS1)
UMR: 103663529(NUS1)
UAH: 113259390(NUS1)
ORO: 101380578(NUS1)
ELK: 111147663
FCA: 101094180(NUS1)
PTG: 102955679(NUS1)
PPAD: 109248467(NUS1)
AJU: 106968326(NUS1)
BTA: 100849670(NUS1)
BOM: 102270031(NUS1)
BIU: 109563493(NUS1)
BBUB: 102409239(NUS1)
CHX: 102173744(NUS1)
OAS: 101102568(NUS1)
SSC: 100154658(NUS1)
CFR: 102506402(NUS1)
CDK: 105088343(NUS1)
BACU: 103004192(NUS1)
LVE: 103082209(NUS1)
OOR: 101271325(NUS1)
DLE: 111164997(NUS1)
PCAD: 102991431(NUS1)
ECB: 100067138(NUS1)
EPZ: 103549504(NUS1)
EAI: 106847018(NUS1)
MYB: 102244874(NUS1)
MYD: 102764339(NUS1)
MNA: 107526295(NUS1)
HAI: 109396468(NUS1)
DRO: 112297035(NUS1)
PALE: 102885564(NUS1)
RAY: 107520185(NUS1)
MJV: 108390217
LAV: 100666506(NUS1)
TMU: 101347937
MDO: 100023437(NUS1)
SHR: 100934010(NUS1)
PCW: 110197587(NUS1)
OAA: 100088240(NUS1)
GGA: 421733(NUS1)
MGP: 100550429(NUS1)
CJO: 107311865(NUS1)
NMEL: 110395566(NUS1)
APLA: 101791531(NUS1)
ACYG: 106047572(NUS1)
TGU: 100187707(NUS1)
LSR: 110476139(NUS1)
SCAN: 103822057(NUS1)
GFR: 102040482(NUS1)
FAB: 101820130(NUS1)
PHI: 102108008(NUS1)
PMAJ: 107202140(NUS1)
CCAE: 111927186(NUS1)
CCW: 104691119(NUS1)
ETL: 114065298(NUS1)
FPG: 101924551(NUS1)
FCH: 102047823(NUS1)
CLV: 102094487(NUS1)
EGZ: 104131963(NUS1)
NNI: 104008829(NUS1)
ACUN: 113477847(NUS1)
PADL: 103918385(NUS1)
AAM: 106497998(NUS1)
ASN: 102387052(NUS1)
AMJ: 102561763(NUS1)
PSS: 102445967(NUS1)
CMY: 102936640(NUS1)
CPIC: 101946143(NUS1)
ACS: 100553689(nus1)
PVT: 110083607(NUS1)
PBI: 103054739(NUS1)
PMUR: 107295731(NUS1)
TSR: 106544536(NUS1)
PMUA: 114594087(NUS1)
GJA: 107108616(NUS1)
XLA: 108716803(nus1.L) 108717920(nus1.S)
XTR: 548844(nus1)
NPR: 108792824(NUS1)
DRE: 436629(nus1)
SRX: 107727674(nus1) 107736830
SGH: 107549681 107603090(nus1)
CCAR: 109065449 109112841(nus1)
IPU: 108257862(nus1)
PHYP: 113531922(nus1)
AMEX: 103039433(nus1)
EEE: 113572279(nus1)
TRU: 101071314(nus1)
LCO: 104939621(nus1)
NCC: 104940985 104957329(nus1)
MZE: 101469329(nus1)
ONL: 100691678(nus1)
OLA: 101169897(nus1)
XMA: 102217942(nus1)
XCO: 114159052(nus1)
PRET: 103458032(nus1)
CVG: 107094775(nus1)
NFU: 107395992(nus1)
KMR: 108228908(nus1)
ALIM: 106518274(nus1)
AOCE: 111585738(nus1)
CSEM: 103380613(nus1)
LCF: 108899340(nus1)
SDU: 111216398(nus1)
HCQ: 109516760(nus1)
BPEC: 110156971(nus1)
MALB: 109966584(nus1)
SASA: 100194740(ngbr) 106607785
ELS: 105017956(nus1)
SFM: 108928556(nus1)
PKI: 111836149(nus1)
LCM: 102351688(NUS1)
CMK: 103182587(nus1)
RTP: 109933903(nus1)
BFO: 118423145
CIN: 100176533
SPU: 100892751
APLC: 110984493
SKO: 100375768
DME: Dmel_CG4775(Tango14)
DER: 6543251
DSE: 6617348
DSI: Dsimw501_GD23075(Dsim_GD23075)
DSR: 110186105
DPE: 6595534
DMN: 108161769
DWI: 6651797
DAZ: 108611457
DNV: 115563641
DHE: 111599471
MDE: 101897427
LCQ: 111681395
AAG: 5580031
AME: 724564
BIM: 100747527
BTER: 100643549
CCAL: 108625059
OBB: 114877138
AEC: 105147263
ACEP: 105625044
PBAR: 105426617
VEM: 105556965
HST: 105183400
DQU: 106749871
CFO: 105249679
LHU: 105670318
PGC: 109863897
OBO: 105284777
PCF: 106784762
NVI: 100116350
CSOL: 105362407
MDL: 103570334
TCA: 661408
DPA: 109535846
NVL: 108559741
BMOR: 101738415
BMAN: 114252448
PMAC: 106708760
PRAP: 110992575
HAW: 110372933
TNL: 113505379
PXY: 105398216
API: 100574241(ACYPI27127)
DNX: 107165308
AGS: 114128632
RMD: 113554167
BTAB: 109040891
CLEC: 106662915
ZNE: 110826612
PVM: 113824156
TUT: 107362134
DPTE: 113790931
BMY: Bm1_55685
TSP: Tsp_08749
PCAN: 112566815
CRG: 105318229
MYI: 110456884
OBI: 106872942
SHX: MS3_09874
NVE: 5513785
EPA: 110251800
ADF: 107335638
AMIL: 114971284
PDAM: 113666726
SPIS: 111328448
DGT: 114536673
HMG: 101236904
GMX: 100818888
SCE: YDL193W(NUS1)
ERC: Ecym_8059
KMX: KLMA_60506(NUS1)
NCS: NCAS_0E03730(NCAS0E03730)
NDI: NDAI_0A02900(NDAI0A02900)
TPF: TPHA_0P00910(TPHA0P00910)
TBL: TBLA_0A05890(TBLA0A05890) TBLA_0F02120(TBLA0F02120)
TDL: TDEL_0C02380(TDEL0C02380)
KAF: KAFR_0L01300(KAFR0L01300)
CAL: CAALFM_C304940WA(CaO19.5963)
SLB: AWJ20_3988(NUS1)
NCR: NCU09312
NTE: NEUTE1DRAFT91460(NEUTE1DRAFT_91460)
MGR: MGG_04560
SSCK: SPSK_05260
MAW: MAC_00557
MAJ: MAA_07252
CMT: CCM_06941
BFU: BCIN_02g03370(Bcnus1)
MBE: MBM_05324
ANI: AN8878.2
ANG: ANI_1_888034(An03g06910)
ABE: ARB_06604
TVE: TRV_04799
PTE: PTT_09135
ABP: AGABI1DRAFT51492(AGABI1DRAFT_51492)
ABV: AGABI2DRAFT197250(AGABI2DRAFT_197250)
MRT: MRET_1901
DFA: DFA_11636
 » show all
Reference
  Authors
Park EJ, Grabinska KA, Guan Z, Stranecky V, Hartmannova H, Hodanova K, Baresova V, Sovova J, Jozsef L, Ondruskova N, Hansikova H, Honzik T, Zeman J, Hulkova H, Wen R, Kmoch S, Sessa WC
  Title
Mutation of Nogo-B receptor, a subunit of cis-prenyltransferase, causes a congenital disorder of glycosylation.
  Journal
Cell Metab 20:448-57 (2014)
DOI:10.1016/j.cmet.2014.06.016
  Sequence
[hsa:116150]
LinkDB

KEGG   ENZYME: 2.5.1.87
Entry
EC 2.5.1.87                 Enzyme                                 

Name
ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific];
RER2;
Rer2p;
Rer2p Z-prenyltransferase;
Srt1p;
Srt2p Z-prenyltransferase;
ACPT;
dehydrodolichyl diphosphate synthase 1
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
Sysname
(2E,6E)-farnesyl-diphosphate:isopentenyl-diphosphate cistransferase (adding 10--55 isopentenyl units)
Reaction(IUBMB)
(2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate = n diphosphate + ditrans,polycis-polyprenyl diphosphate (n = 10--55) [RN:R05556]
Reaction(KEGG)
R05556
Substrate
(2E,6E)-farnesyl diphosphate [CPD:C00448];
isopentenyl diphosphate [CPD:C00129]
Product
diphosphate [CPD:C00013];
ditrans,polycis-polyprenyl diphosphate [CPD:C05859]
Comment
The enzyme is involved in biosynthesis of dolichol (a long-chain polyprenol) with a saturated alpha-isoprene unit, which serves as a glycosyl carrier in protein glycosylation [1]. The yeast Saccharomyces cerevisiae has two different enzymes that catalyse this reaction. Rer2p synthesizes a well-defined family of polyprenols of 13--18 isoprene residues with dominating C(80) (16 isoprene residues) extending to C(120), while Srt1p synthesizes mainly polyprenol with 22 isoprene subunits. Largest Srt1p products reach C(290) [2]. The enzyme from Arabidopsis thaliana catalyses the formation of polyprenyl diphosphates with predominant carbon number C(120) [4].
History
EC 2.5.1.87 created 2010
Pathway
ec00900  Terpenoid backbone biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K11778  ditrans,polycis-polyprenyl diphosphate synthase
K19177  dehydrodolichyl diphosphate syntase complex subunit NUS1
Genes
HSA: 116150(NUS1) 79947(DHDDS)
PTR: 456662(DHDDS) 462965(NUS1) 741304
PPS: 100972313(DHDDS) 100982942(NUS1) 100988367
GGO: 101125977(DHDDS) 101137725(NUS1)
PON: 100171948(DHDDS) 100460016(NUS1)
NLE: 100602333(NUS1) 100603801(DHDDS)
MCC: 714635(DHDDS) 715015(NUS1)
MCF: 101926129(DHDDS) 102126512(NUS1)
CSAB: 103225956(DHDDS) 103240491(NUS1)
RRO: 104654169(NUS1) 104675752(DHDDS)
RBB: 108518187(DHDDS) 108523008(NUS1)
CJC: 100405741(DHDDS) 100412462(NUS1) 100415318
SBQ: 101028238(NUS1) 101046544(DHDDS)
MMU: 52014(Nus1) 67422(Dhdds)
MCAL: 110293104(Dhdds) 110303806(Nus1)
MPAH: 110323123(Dhdds) 110326546(Nus1)
RNO: 294400(Nus1) 298541(Dhdds)
MUN: 110541276(Nus1) 110552313(Dhdds)
CGE: 100761045 100761477(Nus1) 100767025(Dhdds)
NGI: 103736296(Nus1) 103737115(Dhdds)
HGL: 101700845(Nus1) 101711794(Dhdds)
CCAN: 109683062(Dhdds) 109693829(Nus1)
OCU: 100339242(NUS1) 100358963(DHDDS)
TUP: 102478223(NUS1) 102482449(DHDDS)
CFA: 484102(NUS1) 612157(DHDDS)
VVP: 112922764(NUS1) 112931674(DHDDS)
AML: 100463534(DHDDS) 100466277(NUS1)
UMR: 103663529(NUS1) 103666770(DHDDS)
UAH: 113259390(NUS1) 113268456(DHDDS)
ORO: 101375137(DHDDS) 101380578(NUS1)
FCA: 101085905(DHDDS) 101094180(NUS1)
PTG: 102955679(NUS1) 102966133(DHDDS)
PPAD: 109248467(NUS1) 109256774(DHDDS)
AJU: 106968326(NUS1) 106977611(DHDDS)
BTA: 100849670(NUS1) 523264(DHDDS)
BOM: 102270031(NUS1) 102271246(DHDDS)
BIU: 109563493(NUS1) 109573388(DHDDS)
BBUB: 102394454(DHDDS) 102409239(NUS1)
CHX: 102169144(DHDDS) 102173744(NUS1)
OAS: 101102568(NUS1) 101110654(DHDDS)
SSC: 100154658(NUS1) 100524683(DHDDS)
CFR: 102506402(NUS1) 102518547(DHDDS)
CDK: 105088343(NUS1) 105100689(DHDDS)
BACU: 102998703(DHDDS) 103004192(NUS1)
LVE: 103082209(NUS1) 103088197(DHDDS)
OOR: 101271325(NUS1) 101277244(DHDDS)
DLE: 111163358(DHDDS) 111164997(NUS1)
PCAD: 102991431(NUS1) 102994453(DHDDS)
ECB: 100057242(DHDDS) 100067138(NUS1)
EPZ: 103549504(NUS1) 103550540(DHDDS)
EAI: 106839917(DHDDS) 106847018(NUS1)
MYB: 102238802(DHDDS) 102244874(NUS1)
MYD: 102758520(DHDDS) 102764339(NUS1)
MNA: 107526295(NUS1) 107539928(DHDDS)
HAI: 109386757(DHDDS) 109396468(NUS1)
DRO: 112297035(NUS1) 112299245(DHDDS)
PALE: 102885564(NUS1) 102891420(DHDDS)
RAY: 107503570(DHDDS) 107520185(NUS1)
LAV: 100666506(NUS1) 100675778(DHDDS)
MDO: 100012356(DHDDS) 100023437(NUS1)
SHR: 100922631(DHDDS) 100934010(NUS1)
PCW: 110197587(NUS1) 110206127(DHDDS)
OAA: 100083017(DHDDS) 100088240(NUS1)
GGA: 421733(NUS1)
MGP: 100541233(DHDDS) 100550429(NUS1)
CJO: 107311865(NUS1) 107324014(DHDDS)
NMEL: 110387387(DHDDS) 110395566(NUS1)
APLA: 101791531(NUS1) 101804205(DHDDS)
ACYG: 106047572(NUS1) 106047921(DHDDS)
TGU: 100187707(NUS1) 100230447(DHDDS)
LSR: 110472863(DHDDS) 110476139(NUS1)
SCAN: 103822057(NUS1) 103823525(DHDDS)
GFR: 102038851(DHDDS) 102040482(NUS1)
FAB: 101816671(DHDDS) 101820130(NUS1)
PHI: 102105638(DHDDS) 102108008(NUS1)
PMAJ: 107202140(NUS1) 107214256(DHDDS)
CCAE: 111927186(NUS1) 111939089(DHDDS)
CCW: 104691119(NUS1) 104694634(DHDDS)
ETL: 114065298(NUS1) 114068054(DHDDS)
FPG: 101919832(DHDDS) 101924551(NUS1)
FCH: 102047823(NUS1) 102049798(DHDDS)
CLV: 102094487(NUS1) 102098335(DHDDS)
EGZ: 104131963(NUS1) 104134887(DHDDS)
NNI: 104008829(NUS1) 104017719(DHDDS)
ACUN: 113477847(NUS1) 113488396(DHDDS)
PADL: 103918385(NUS1) 103925863(DHDDS)
AAM: 106485593(DHDDS) 106497998(NUS1)
ASN: 102374803(DHDDS) 102387052(NUS1)
AMJ: 102557872(DHDDS) 102561763(NUS1)
PSS: 102444519(DHDDS) 102445967(NUS1)
CMY: 102936640(NUS1) 102941423(DHDDS)
CPIC: 101946143(NUS1) 101949942(DHDDS)
ACS: 100552810(dhdds) 100553689(nus1)
PVT: 110083607(NUS1) 110086158(DHDDS)
PBI: 103054739(NUS1) 103067643(DHDDS)
PMUR: 107293795(DHDDS) 107295731(NUS1)
TSR: 106544536(NUS1) 106547071(DHDDS)
PMUA: 114594087(NUS1) 114601527(DHDDS)
GJA: 107108616(NUS1) 107110426(DHDDS)
XLA: 108716803(nus1.L) 108717920(nus1.S) 379162(dhdds.S) 398626(dhdds.L)
XTR: 100135207(dhdds) 548844(nus1)
NPR: 108792824(NUS1) 108800534(DHDDS)
DRE: 406468(dhdds) 436629(nus1)
CCAR: 109065449 109112841(nus1)
IPU: 108256361(dhdds) 108257862(nus1)
PHYP: 113531922(nus1) 113533089(dhdds)
AMEX: 103039433(nus1) 103040074(dhdds)
EEE: 113572279(nus1) 113583554(dhdds)
TRU: 101067613(dhdds) 101071314(nus1)
LCO: 104924709(dhdds) 104939621(nus1)
MZE: 101465746(dhdds) 101469329(nus1)
ONL: 100691678(nus1) 100705873(dhdds)
OLA: 101156768(dhdds) 101169897(nus1)
XMA: 102217942(nus1) 102232893(dhdds)
XCO: 114141550(dhdds) 114159052(nus1)
PRET: 103458032(nus1) 103477772(dhdds)
CVG: 107082951(dhdds) 107094775(nus1)
NFU: 107379325(dhdds) 107395992(nus1)
KMR: 108228908(nus1) 108240318(dhdds)
ALIM: 106518274(nus1) 106532228(dhdds)
AOCE: 111578588(dhdds) 111585738(nus1)
CSEM: 103380613(nus1) 103388088(dhdds)
LCF: 108899340(nus1) 108899452(dhdds)
SDU: 111216398(nus1) 111235772(dhdds)
HCQ: 109515435(dhdds) 109516760(nus1)
BPEC: 110156971(nus1) 110174299(dhdds)
MALB: 109954705(dhdds) 109966584(nus1)
ELS: 105017956(nus1) 105028368(dhdds)
SFM: 108919101(dhdds) 108928556(nus1)
PKI: 111836149(nus1) 111844563(dhdds)
LCM: 102351688(NUS1) 102361662(DHDDS)
CMK: 103179564(dhdds) 103182587(nus1)
RTP: 109933903(nus1) 109937745(dhdds)
DME: Dmel_CG10778(CG10778) Dmel_CG4775(Tango14)
DSI: Dsimw501_GD16139(Dsim_GD16139) Dsimw501_GD23075(Dsim_GD23075)
DAN: 6503683
DVI: 6631360
ATD: 109603363
BMOR: 101738415 732991(Dhdds)
API: 100161888 100164640 100574241(ACYPI27127)
CEL: CELE_T01G1.4(T01G1.4)
EGL: EGR_09410
ATH: AT1G11755(LEW1) AT2G17570(cPT1) AT2G23400 AT2G23410(CPT) AT5G58770(cPT4) AT5G58780(cPT5) AT5G58782(cPT6) AT5G58784 AT5G60500(cPT8) AT5G60510(cPT9)
LJA: Lj0g3v0314369.1(Lj0g3v0314369.1) Lj0g3v0326269.1(Lj0g3v0326269.1) Lj0g3v0326269.3(Lj0g3v0326269.3) Lj1g3v0131170.1(Lj1g3v0131170.1)
SLY: 101244138(CPT4) 101250227(SlCPTBP) 101253666(CPT3) 101256886(CPT7) 101267417 104644439
DOSA: Os01t0857200-01(Os01g0857200) Os02t0197700-01(Os02g0197700) Os03t0197000-01(Os03g0197000) Os06t0167400-01(Os06g0167400) Os07t0607700-01(Os07g0607700)
ATS: 109742971(LOC109742971) 109757670(LOC109757670) 109757672(LOC109757672) 109757676(LOC109757676) 109759695(LOC109759695) 109762622 109769504 109772847(LOC109772847)
APRO: F751_3077
SCE: YBR002C(RER2) YDL193W(NUS1) YMR101C(SRT1)
KMX: KLMA_30092(SRT1) KLMA_60115(RER2) KLMA_60506(NUS1)
NCS: NCAS_0E01380(NCAS0E01380) NCAS_0E03730(NCAS0E03730) NCAS_0F01440(NCAS0F01440)
NDI: NDAI_0A02900(NDAI0A02900) NDAI_0G01550(NDAI0G01550) NDAI_0H02320(NDAI0H02320)
TPF: TPHA_0A04470(TPHA0A04470) TPHA_0G02950(TPHA0G02950) TPHA_0P00910(TPHA0P00910)
TBL: TBLA_0A05890(TBLA0A05890) TBLA_0B08960(TBLA0B08960) TBLA_0F00550(TBLA0F00550) TBLA_0F02120(TBLA0F02120)
TDL: TDEL_0A02280(TDEL0A02280) TDEL_0C02380(TDEL0C02380) TDEL_0D04190(TDEL0D04190)
KAF: KAFR_0A00610(KAFR0A00610) KAFR_0F02540(KAFR0F02540) KAFR_0L01300(KAFR0L01300)
CAL: CAALFM_C112270WA(SRT1) CAALFM_C304940WA(CaO19.5963) CAALFM_C505330CA(RER2)
SLB: AWJ20_395(RER2) AWJ20_3988(NUS1)
NTE: NEUTE1DRAFT84674(NEUTE1DRAFT_84674) NEUTE1DRAFT91460(NEUTE1DRAFT_91460)
BFU: BCIN_02g03370(Bcnus1) BCIN_05g03890(Bcrer2)
ANG: ANI_1_1896184(An04g05250) ANI_1_888034(An03g06910)
CNE: CND03430
CNB: CNBD2910
TASA: A1Q1_02236
ABP: AGABI1DRAFT110974(AGABI1DRAFT_110974) AGABI1DRAFT51492(AGABI1DRAFT_51492)
ABV: AGABI2DRAFT190174(AGABI2DRAFT_190174) AGABI2DRAFT197250(AGABI2DRAFT_197250)
MGL: MGL_1818
PCB: PCHAS_093380(PC000683.01.0)
TAN: TA04455
TPV: TP03_0421
BBO: BBOV_IV002310(21.m02978)
 » show all
Reference
1  [PMID:11442630]
  Authors
Sato M, Fujisaki S, Sato K, Nishimura Y, Nakano A
  Title
Yeast Saccharomyces cerevisiae has two cis-prenyltransferases with different properties and localizations. Implication for their distinct physiological roles  in dolichol synthesis.
  Journal
Genes Cells 6:495-506 (2001)
DOI:10.1046/j.1365-2443.2001.00438.x
  Sequence
Reference
2  [PMID:17345630]
  Authors
Poznanski J, Szkopinska A
  Title
Precise bacterial polyprenol length control fails in Saccharomyces cerevisiae.
  Journal
Biopolymers 86:155-64 (2007)
DOI:10.1002/bip.20715
  Sequence
Reference
3  [PMID:9858571]
  Authors
Sato M, Sato K, Nishikawa S, Hirata A, Kato J, Nakano A.
  Title
The yeast RER2 gene, identified by endoplasmic reticulum protein localization mutations, encodes cis-prenyltransferase, a key enzyme in dolichol synthesis.
  Journal
Mol Cell Biol 19:471-83 (1999)
DOI:10.1128/MCB.19.1.471
  Sequence
Reference
4  [PMID:10764783]
  Authors
Oh SK, Han KH, Ryu SB, Kang H
  Title
Molecular cloning, expression, and functional analysis of a cis-prenyltransferase from Arabidopsis thaliana. Implications in rubber biosynthesis.
  Journal
J Biol Chem 275:18482-8 (2000)
DOI:10.1074/jbc.M002000200
  Sequence
[ath:AT2G23410]
Reference
5  [PMID:10908715]
  Authors
Cunillera N, Arro M, Fores O, Manzano D, Ferrer A
  Title
Characterization of dehydrodolichyl diphosphate synthase of Arabidopsis thaliana, a key enzyme in dolichol biosynthesis.
  Journal
FEBS Lett 477:170-4 (2000)
DOI:10.1016/S0014-5793(00)01798-1
  Sequence
[ath:AT2G23410]
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.87
IUBMB Enzyme Nomenclature: 2.5.1.87
ExPASy - ENZYME nomenclature database: 2.5.1.87
BRENDA, the Enzyme Database: 2.5.1.87
LinkDB

KEGG   REACTION: R05556
Entry
R05556                      Reaction                               

Name
(2E,6E)-farnesyl-diphosphate:isopentenyl-diphosphate cistransferase (adding 10-55 isopentenyl units)
Definition
trans,trans-Farnesyl diphosphate + n Isopentenyl diphosphate <=> Dehydrodolichol diphosphate + n Diphosphate
Equation
C00448 + n C00129 <=> C05859 + n C00013
Comment
multistep-reaction (n = 10-55)
Reaction class
RC00279  C00129_C05859
RC02839  C00448_C05859
Enzyme
Pathway
rn00900  Terpenoid backbone biosynthesis
rn01110  Biosynthesis of secondary metabolites
Orthology
K11778  ditrans,polycis-polyprenyl diphosphate synthase [EC:2.5.1.87]
K19177  dehydrodolichyl diphosphate syntase complex subunit NUS1 [EC:2.5.1.87]
Other DBs
RHEA: 53011
LinkDB

DBGET integrated database retrieval system