KEGG   ORTHOLOGY: K12711Help
Entry
K12711                      KO                                     

Name
novM, cloM, couM
Definition
L-demethylnoviosyl transferase [EC:2.4.1.302]
Pathway
ko00401  Novobiocin biosynthesis
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
ko01130  Biosynthesis of antibiotics
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00401 Novobiocin biosynthesis
    K12711  novM, cloM, couM; L-demethylnoviosyl transferase
Enzymes [BR:ko01000]
 2. Transferases
  2.4  Glycosyltransferases
   2.4.1  Hexosyltransferases
    2.4.1.302  L-demethylnoviosyl transferase
     K12711  novM, cloM, couM; L-demethylnoviosyl transferase
BRITE hierarchy
Other DBs
RN: R06767 R06770 R06773
CAZy: GT1
Genes
NGV: CDO52_02205
AG: AAF67506(novM) AAG29785(couM) AAN65229(cloM)
TaxonomyKoalaUniProt
Reference
  Authors
Steffensky M, Muhlenweg A, Wang ZX, Li SM, Heide L
  Title
Identification of the novobiocin biosynthetic gene cluster of Streptomyces spheroides NCIB 11891.
  Journal
Antimicrob Agents Chemother 44:1214-22 (2000)
DOI:10.1128/AAC.44.5.1214-1222.2000
  Sequence
Reference
  Authors
Pojer F, Li SM, Heide L.
  Title
Molecular cloning and sequence analysis of the clorobiocin biosynthetic gene cluster: new insights into the biosynthesis of aminocoumarin antibiotics.
  Journal
Microbiology 148:3901-11 (2002)
DOI:10.1099/00221287-148-12-3901
  Sequence
Reference
  Authors
Freel Meyers CL, Oberthur M, Heide L, Kahne D, Walsh CT
  Title
Assembly of dimeric variants of coumermycins by tandem action of the four biosynthetic enzymes CouL, CouM, CouP, and NovN.
  Journal
Biochemistry 43:15022-36 (2004)
DOI:10.1021/bi048457z
LinkDB All DBs

KEGG   ENZYME: 2.4.1.302Help
Entry
EC 2.4.1.302                Enzyme                                 

Name
L-demethylnoviosyl transferase;
novM (gene name);
dTDP-beta-L-noviose:novobiocic acid 7-O-noviosyltransferase;
L-noviosyl transferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
dTDP-4-O-demethyl-beta-L-noviose:novobiocic acid 7-O-[4-O-demethyl-L-noviosyl]transferase
Reaction(IUBMB)
dTDP-4-O-demethyl-beta-L-noviose + novobiocic acid = dTDP + demethyldecarbamoyl novobiocin [RN:R06770]
Reaction(KEGG)
Substrate
dTDP-4-O-demethyl-beta-L-noviose [CPD:C12481];
novobiocic acid [CPD:C12474]
Product
dTDP [CPD:C00363];
demethyldecarbamoyl novobiocin
Comment
The enzyme is involved in the biosynthesis of the aminocoumarin antibiotic, novobiocin.
History
EC 2.4.1.302 created 2013, modified 2016
Pathway
ec00401  Novobiocin biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
ec01130  Biosynthesis of antibiotics
Orthology
K12711  L-demethylnoviosyl transferase
Genes
NGV: CDO52_02205
Taxonomy
Reference
1  [PMID:12680772]
  Authors
Freel Meyers CL, Oberthur M, Anderson JW, Kahne D, Walsh CT
  Title
Initial characterization of novobiocic acid noviosyl transferase activity of NovM in biosynthesis of the antibiotic novobiocin.
  Journal
Biochemistry 42:4179-89 (2003)
DOI:10.1021/bi0340088
  Sequence
Reference
2  [PMID:12633109]
  Authors
Albermann C, Soriano A, Jiang J, Vollmer H, Biggins JB, Barton WA, Lesniak J, Nikolov DB, Thorson JS
  Title
Substrate specificity of NovM: implications for novobiocin biosynthesis and glycorandomization.
  Journal
Org Lett 5:933-6 (2003)
DOI:10.1021/ol0341086
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.302
IUBMB Enzyme Nomenclature: 2.4.1.302
ExPASy - ENZYME nomenclature database: 2.4.1.302
BRENDA, the Enzyme Database: 2.4.1.302
LinkDB All DBs

KEGG   REACTION: R06770Help
Entry
R06770                      Reaction                               

Name
dTDP-4-O-demethyl-beta-L-noviose:novobiocic-acid 7-O-[4-O-demethyl-L-noviosyl]transferase
Definition
Novobiocic acid + dTDP-5-dimethyl-L-lyxose <=> Demethyldecarbamoylnovobiocin + dTDP
Equation
Reaction class
RC00005  C00363_C12481
RC00171  C12474_C12475
Enzyme
Pathway
rn00401  Novobiocin biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
rn01130  Biosynthesis of antibiotics
Orthology
K12711  L-demethylnoviosyl transferase [EC:2.4.1.302]
Other DBs
RHEA: 36698
LinkDB All DBs

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