KEGG   ORTHOLOGY: K12742
Entry
K12742                      KO                                     

Name
ispS
Definition
isoprene synthase [EC:4.2.3.27]
Pathway
ko00900  Terpenoid backbone biosynthesis
ko01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00900 Terpenoid backbone biosynthesis
    K12742  ispS; isoprene synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.27  isoprene synthase
     K12742  ispS; isoprene synthase
Other DBs
RN: R08199
GO: 0034009
Genes
ALY: 9306207
CRB: 17880730
CSAT: 104718686 104723429 104731915
BRP: 103869246
BNA: 106358198 106437444
BOE: 106301302
RSZ: 108834872 108835531
THJ: 104809623 104813173 104814037 104817852
CIT: 102613157 102614425 102626262
GRA: 105780734 105780735
GAB: 108459891
GMX: 100782452(GMTPS3) 100786684(GMTPS23) 100801629(GMTPS8) 100814880(GMTPS9)
LJA: Lj0g3v0356449.1(Lj0g3v0356449.1) Lj3g3v2888440.1(Lj3g3v2888440.1) Lj3g3v2888450.1(Lj3g3v2888450.1)
LANG: 109355202
MDM: 103404781
CMO: 103499736
RCU: 8260996
SIND: 105165226
CCAV: 112529037
ATS: 109735704(LOC109735704) 109764078(LOC109764078) 109777110(LOC109777110)
PEQ: 110032081
ATR: 18436933
AG: BAD98243(ISPS) CAC35696(ISPS)
 » show all
Reference
PMID:11506373 (Populus tremula x Populus alba)
  Authors
Miller B, Oschinski C, Zimmer W
  Title
First isolation of an isoprene synthase gene from poplar and successful expression of the gene in Escherichia coli.
  Journal
Planta 213:483-7 (2001)
DOI:10.1007/s004250100557
  Sequence
LinkDB

KEGG   ENZYME: 4.2.3.27
Entry
EC 4.2.3.27                 Enzyme                                 

Name
isoprene synthase;
ISPC;
ISPS
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
Sysname
dimethylallyl-diphosphate diphosphate-lyase (isoprene-forming)
Reaction(IUBMB)
dimethylallyl diphosphate = isoprene + diphosphate [RN:R08199]
Reaction(KEGG)
R08199
Substrate
dimethylallyl diphosphate [CPD:C00235]
Product
isoprene [CPD:C16521];
diphosphate [CPD:C00013]
Comment
Requires Mg2+ or Mn2+ for activity. This enzyme is located in the chloroplast of isoprene-emitting plants, such as poplar and aspen, and may be activitated by light-dependent changes in chloroplast pH and Mg2+ concentration [2,8].
History
EC 4.2.3.27 created 2007
Pathway
ec00900  Terpenoid backbone biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K12742  isoprene synthase
Genes
ALY: 9306207
CRB: 17880730
CSAT: 104718686 104723429 104731915
BRP: 103869246
BNA: 106358198 106437444
BOE: 106301302
RSZ: 108834872 108835531
THJ: 104809623 104813173 104814037 104817852
CIT: 102613157 102614425 102626262
GRA: 105780734 105780735
GAB: 108459891
GMX: 100782452(GMTPS3) 100786684(GMTPS23) 100801629(GMTPS8) 100814880(GMTPS9)
LJA: Lj0g3v0356449.1(Lj0g3v0356449.1) Lj3g3v2888440.1(Lj3g3v2888440.1) Lj3g3v2888450.1(Lj3g3v2888450.1)
LANG: 109355202
MDM: 103404781
CMO: 103499736
RCU: 8260996
SIND: 105165226
CCAV: 112529037
ATS: 109735704(LOC109735704) 109764078(LOC109764078) 109777110(LOC109777110)
PEQ: 110032081
ATR: 18436933
 » show all
Reference
1  [PMID:16668590]
  Authors
Silver GM, Fall R.
  Title
Enzymatic Synthesis of Isoprene from Dimethylallyl Diphosphate in Aspen Leaf Extracts.
  Journal
Plant Physiol 97:1588-1591 (1991)
DOI:10.1104/pp.97.4.1588
Reference
2  [PMID:7768893]
  Authors
Silver GM, Fall R.
  Title
Characterization of aspen isoprene synthase, an enzyme responsible for leaf isoprene emission to the atmosphere.
  Journal
J Biol Chem 270:13010-6 (1995)
DOI:10.1074/jbc.270.22.13010
Reference
3  [PMID:12226383]
  Authors
Wildermuth MC, Fall R.
  Title
Light-Dependent Isoprene Emission (Characterization of a Thylakoid-Bound Isoprene Synthase in Salix discolor Chloroplasts).
  Journal
Plant Physiol 112:171-182 (1996)
DOI:10.1104/pp.112.1.171
Reference
4
  Authors
Schnitzler, J.P., Arenz, R., Steinbrecher, R. and Lehming, A.
  Title
Characterization of an isoprene synthase from leaves of Quercus petraea.
  Journal
Bot Acta 109:216-221 (1996)
Reference
5  [PMID:11506373]
  Authors
Miller B, Oschinski C, Zimmer W.
  Title
First isolation of an isoprene synthase gene from poplar and successful expression of the gene in Escherichia coli.
  Journal
Planta 213:483-7 (2001)
DOI:10.1007/s004250100557
  Sequence
Reference
6  [PMID:12054742]
  Authors
Sivy TL, Shirk MC, Fall R.
  Title
Isoprene synthase activity parallels fluctuations of isoprene release during growth of Bacillus subtilis.
  Journal
Biochem Biophys Res Commun 294:71-5 (2002)
DOI:10.1016/S0006-291X(02)00435-7
Reference
7  [PMID:15848197]
  Authors
Sasaki K, Ohara K, Yazaki K.
  Title
Gene expression and characterization of isoprene synthase from Populus alba.
  Journal
FEBS Lett 579:2514-8 (2005)
DOI:10.1016/j.febslet.2005.03.066
  Sequence
Reference
8  [PMID:16052321]
  Authors
Schnitzler JP, Zimmer I, Bachl A, Arend M, Fromm J, Fischbach RJ.
  Title
Biochemical properties of isoprene synthase in poplar (Populus x canescens).
  Journal
Planta 222:777-86 (2005)
DOI:10.1007/s00425-005-0022-1
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.27
IUBMB Enzyme Nomenclature: 4.2.3.27
ExPASy - ENZYME nomenclature database: 4.2.3.27
BRENDA, the Enzyme Database: 4.2.3.27
CAS: 139172-14-8
LinkDB

KEGG   REACTION: R08199
Entry
R08199                      Reaction                               

Name
dimethylallyl-diphosphate diphosphate-lyase (isoprene-forming)
Definition
Dimethylallyl diphosphate <=> Isoprene + Diphosphate
Equation
Reaction class
RC00637  C00235_C16521
Enzyme
Pathway
rn00900  Terpenoid backbone biosynthesis
rn01110  Biosynthesis of secondary metabolites
Orthology
K12742  isoprene synthase [EC:4.2.3.27]
Other DBs
RHEA: 13372
LinkDB

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