KEGG   ORTHOLOGY: K14451
Entry
K14451                      KO                                     

Name
mcl2
Definition
(3S)-malyl-CoA thioesterase [EC:3.1.2.30]
Pathway
ko00630  Glyoxylate and dicarboxylate metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
Module
M00373  Ethylmalonyl pathway
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09101 Carbohydrate metabolism
   00630 Glyoxylate and dicarboxylate metabolism
    K14451  mcl2; (3S)-malyl-CoA thioesterase
Enzymes [BR:ko01000]
 3. Hydrolases
  3.1  Acting on ester bonds
   3.1.2  Thioester hydrolases
    3.1.2.30  (3S)-malyl-CoA thioesterase
     K14451  mcl2; (3S)-malyl-CoA thioesterase
Other DBs
RN: R10612
COG: COG2301
Genes
BOJ: CBF45_15340
KGY: EHF36_15360
CPAF: C6V80_02765
BRD: JL11_15545
BRG: A4249_01015
BVC: CEP68_03750
BREV: E7T10_13660
BVY: NCTC9239_00695(mcl2)
AEX: Astex_0375
SIL: SPO0352(citE)
RUT: FIU92_01320(mcl1)
RSP: RSP_0970
RCP: RCAP_rcc00727(citE1)
JAN: Jann_0816
RDE: RD1_1620(citE)
PDE: Pden_0563
DSH: Dshi_2874
PGD: Gal_03134
OTM: OSB_04760(mcl2)
LAQU: R2C4_13635
PTP: RCA23_c14020(citE)
CID: P73_3868
MALG: MALG_02745
RSU: NHU_00724(citE)
RHC: RGUI_0622
SPSE: SULPSESMR1_00394(mcl2)
RMM: ROSMUCSMR3_02505(mcl2)
RID: RIdsm_04538(mcl2)
ROH: FIU89_19180(mcl1)
LVS: LOKVESSMR4R_02803(mcl2)
AHT: ANTHELSMS3_02127(mcl2)
MARU: FIU81_04115(mcl1)
ROT: FIV09_01380(mcl1)
MALU: KU6B_41840(mcl2)
SPHP: LH20_16080
SMAZ: LH19_01485
SGI: SGRAN_0950(mcl2)
SPHU: SPPYR_0495(mcl)
SPHI: TS85_09155
SSAN: NX02_12375
SJP: SJA_C1-26890(citE)
SSY: SLG_14830
SPMI: K663_04105
SPHB: EP837_00844(citE)
SPHR: BSY17_744
SINB: SIDU_01380
SPHT: K426_06735
DGO: DGo_CA0363(citE)
DFC: DFI_10115
BLQ: L21SP5_01621(mcl2)
 » show all
Reference
  Authors
Alber BE
  Title
Biotechnological potential of the ethylmalonyl-CoA pathway.
  Journal
Appl Microbiol Biotechnol 89:17-25 (2011)
DOI:10.1007/s00253-010-2873-z
Reference
  Authors
Erb TJ, Frerichs-Revermann L, Fuchs G, Alber BE
  Title
The apparent malate synthase activity of Rhodobacter sphaeroides is due to two paralogous enzymes, (3S)-Malyl-coenzyme A (CoA)/{beta}-methylmalyl-CoA lyase and (3S)- Malyl-CoA thioesterase.
  Journal
J Bacteriol 192:1249-58 (2010)
DOI:10.1128/JB.01267-09
  Sequence
[rsp:RSP_0970]
LinkDB

KEGG   ORTHOLOGY: K19282
Entry
K19282                      KO                                     

Name
aceB
Definition
bifunctional (S)-malyl-CoA lyase/thioesterase [EC:4.1.3.24 3.1.2.30]
Pathway
ko00630  Glyoxylate and dicarboxylate metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
Module
M00012  Glyoxylate cycle
M00740  Methylaspartate cycle
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09101 Carbohydrate metabolism
   00630 Glyoxylate and dicarboxylate metabolism
    K19282  aceB; bifunctional (S)-malyl-CoA lyase/thioesterase
Enzymes [BR:ko01000]
 3. Hydrolases
  3.1  Acting on ester bonds
   3.1.2  Thioester hydrolases
    3.1.2.30  (3S)-malyl-CoA thioesterase
     K19282  aceB; bifunctional (S)-malyl-CoA lyase/thioesterase
 4. Lyases
  4.1  Carbon-carbon lyases
   4.1.3  Oxo-acid-lyases
    4.1.3.24  malyl-CoA lyase
     K19282  aceB; bifunctional (S)-malyl-CoA lyase/thioesterase
Other DBs
RN: R00473 R10612
GO: 0050083
Genes
HDL: HALDL1_15310
HHB: Hhub_3380(aceB)
HSU: HLASF_2007(aceB)
HSF: HLASA_1993(aceB)
SALR: FQU85_05715
HMA: rrnAC1965(aceB)
HHI: HAH_2476(aceB)
HHN: HISP_12605
HAB: SG26_04910
HTA: BVU17_12045
NPH: NP_4430A(aceB)
NMO: Nmlp_2357(aceB)
HWA: HQ_1720A(aceB)
HWC: Hqrw_1842(aceB)
HVO: HVO_1983(aceB1) HVO_B0200(aceB2)
HME: HFX_2078(aceB)
HDF: AArcSl_1031(aceB)
HTU: Htur_4695
NMG: Nmag_3363(aceB)
 » show all
Reference
  Authors
Khomyakova M, Bukmez O, Thomas LK, Erb TJ, Berg IA
  Title
A methylaspartate cycle in haloarchaea.
  Journal
Science 331:334-7 (2011)
DOI:10.1126/science.1196544
  Sequence
[hma:rrnAC1965]
LinkDB

KEGG   ENZYME: 3.1.2.30
Entry
EC 3.1.2.30                 Enzyme                                 

Name
(3S)-malyl-CoA thioesterase;
mcl2 (gene name)
Class
Hydrolases;
Acting on ester bonds;
Thioester hydrolases
Sysname
(S)-malyl-CoA hydrolase
Reaction(IUBMB)
(S)-malyl-CoA + H2O = (S)-malate + CoA [RN:R10612]
Reaction(KEGG)
R10612
Substrate
(S)-malyl-CoA [CPD:C04348];
H2O [CPD:C00001]
Product
(S)-malate [CPD:C00149];
CoA [CPD:C00010]
Comment
Stimulated by Mg2+ or Mn2+. The enzyme has no activity with (2R,3S)-2-methylmalyl-CoA (cf. EC 4.1.3.24, malyl-CoA lyase) or other CoA esters.
History
EC 3.1.2.30 created 2014
Pathway
ec00630  Glyoxylate and dicarboxylate metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K14451  (3S)-malyl-CoA thioesterase
K19282  bifunctional (S)-malyl-CoA lyase/thioesterase
Genes
BOJ: CBF45_15340
KGY: EHF36_15360
CPAF: C6V80_02765
BRD: JL11_15545
BRG: A4249_01015
BVC: CEP68_03750
BREV: E7T10_13660
BVY: NCTC9239_00695(mcl2)
AEX: Astex_0375
SIL: SPO0352(citE)
RUT: FIU92_01320(mcl1)
RSP: RSP_0970
RCP: RCAP_rcc00727(citE1)
JAN: Jann_0816
RDE: RD1_1620(citE)
PDE: Pden_0563
DSH: Dshi_2874
PGD: Gal_03134
OTM: OSB_04760(mcl2)
LAQU: R2C4_13635
PTP: RCA23_c14020(citE)
CID: P73_3868
MALG: MALG_02745
RSU: NHU_00724(citE)
RHC: RGUI_0622
SPSE: SULPSESMR1_00394(mcl2)
RMM: ROSMUCSMR3_02505(mcl2)
RID: RIdsm_04538(mcl2)
ROH: FIU89_19180(mcl1)
LVS: LOKVESSMR4R_02803(mcl2)
AHT: ANTHELSMS3_02127(mcl2)
MARU: FIU81_04115(mcl1)
ROT: FIV09_01380(mcl1)
MALU: KU6B_41840(mcl2)
SPHP: LH20_16080
SMAZ: LH19_01485
SGI: SGRAN_0950(mcl2)
SPHU: SPPYR_0495(mcl)
SPHI: TS85_09155
SSAN: NX02_12375
SJP: SJA_C1-26890(citE)
SSY: SLG_14830
SPMI: K663_04105
SPHB: EP837_00844(citE)
SPHR: BSY17_744
SINB: SIDU_01380
SPHT: K426_06735
DGO: DGo_CA0363(citE)
DFC: DFI_10115
BLQ: L21SP5_01621(mcl2)
HHB: Hhub_3380(aceB)
HSU: HLASF_2007(aceB)
HSF: HLASA_1993(aceB)
HMA: rrnAC1965(aceB)
HHI: HAH_2476(aceB)
NPH: NP_4430A(aceB)
NMO: Nmlp_2357(aceB)
HWA: HQ_1720A(aceB)
HWC: Hqrw_1842(aceB)
HVO: HVO_1983(aceB1) HVO_B0200(aceB2)
HME: HFX_2078(aceB)
HDF: AArcSl_1031(aceB)
HTU: Htur_4695
NMG: Nmag_3363(aceB)
 » show all
Reference
1  [PMID:20047909]
  Authors
Erb TJ, Frerichs-Revermann L, Fuchs G, Alber BE
  Title
The apparent malate synthase activity of Rhodobacter sphaeroides is due to two paralogous enzymes, (3S)-Malyl-coenzyme A (CoA)/{beta}-methylmalyl-CoA lyase and  (3S)- Malyl-CoA thioesterase.
  Journal
J Bacteriol 192:1249-58 (2010)
DOI:10.1128/JB.01267-09
  Sequence
[rsp:RSP_0970]
Other DBs
ExplorEnz - The Enzyme Database: 3.1.2.30
IUBMB Enzyme Nomenclature: 3.1.2.30
ExPASy - ENZYME nomenclature database: 3.1.2.30
BRENDA, the Enzyme Database: 3.1.2.30
LinkDB

KEGG   REACTION: R10612
Entry
R10612                      Reaction                               

Name
(S)-malyl-CoA hydrolase
Definition
L-Malyl-CoA + H2O <=> (S)-Malate + CoA
Equation
Reaction class
RC00004  C00010_C04348
RC00014  C00149_C04348
Enzyme
Pathway
rn00630  Glyoxylate and dicarboxylate metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01200  Carbon metabolism
Module
M00012  Glyoxylate cycle
M00373  Ethylmalonyl pathway
M00740  Methylaspartate cycle
Orthology
K14451  (3S)-malyl-CoA thioesterase [EC:3.1.2.30]
K19282  bifunctional (S)-malyl-CoA lyase/thioesterase [EC:4.1.3.24 3.1.2.30]
Other DBs
RHEA: 38294
LinkDB

DBGET integrated database retrieval system