KEGG   ORTHOLOGY: K14579
Entry
K14579                      KO                                     

Name
nahAc, ndoB, nbzAc, dntAc
Definition
naphthalene 1,2-dioxygenase subunit alpha [EC:1.14.12.12 1.14.12.23 1.14.12.24]
Pathway
ko00624  Polycyclic aromatic hydrocarbon degradation
ko00626  Naphthalene degradation
ko00627  Aminobenzoate degradation
ko00633  Nitrotoluene degradation
ko00642  Ethylbenzene degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01220  Degradation of aromatic compounds
Module
M00534  Naphthalene degradation, naphthalene => salicylate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00627 Aminobenzoate degradation
    K14579  nahAc, ndoB, nbzAc, dntAc; naphthalene 1,2-dioxygenase subunit alpha
   00633 Nitrotoluene degradation
    K14579  nahAc, ndoB, nbzAc, dntAc; naphthalene 1,2-dioxygenase subunit alpha
   00642 Ethylbenzene degradation
    K14579  nahAc, ndoB, nbzAc, dntAc; naphthalene 1,2-dioxygenase subunit alpha
   00626 Naphthalene degradation
    K14579  nahAc, ndoB, nbzAc, dntAc; naphthalene 1,2-dioxygenase subunit alpha
   00624 Polycyclic aromatic hydrocarbon degradation
    K14579  nahAc, ndoB, nbzAc, dntAc; naphthalene 1,2-dioxygenase subunit alpha
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.12  With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
    1.14.12.12  naphthalene 1,2-dioxygenase
     K14579  nahAc, ndoB, nbzAc, dntAc; naphthalene 1,2-dioxygenase subunit alpha
    1.14.12.23  nitroarene dioxygenase
     K14579  nahAc, ndoB, nbzAc, dntAc; naphthalene 1,2-dioxygenase subunit alpha
    1.14.12.24  2,4-dinitrotoluene dioxygenase
     K14579  nahAc, ndoB, nbzAc, dntAc; naphthalene 1,2-dioxygenase subunit alpha
Other DBs
RN: R02968 R05422 R05423 R05424 R05425 R05426 R05427 R06909 R06930 R06937 R07704 R07706 R09159 R09233
GO: 0018625
Genes
PPI: ND055(nahAc)
PSC: A458_06725
PSTU: UIB01_22625
PBM: CL52_14610
PFK: PFAS1_28610
ALT: ambt_17895
BMK: DM80_1443(ndoB)
PARB: CJU94_38660
PNA: Pnap_2485
AJS: Ajs_3092
AG: AAB09766(dntAc) AAL76202(nbzAc) AAO64274(nahAc) ADQ90222(cnbAc) AGH09226(mntAc)
 » show all
Reference
PMID:8486285
  Authors
Simon MJ, Osslund TD, Saunders R, Ensley BD, Suggs S, Harcourt A, Suen WC, Cruden DL, Gibson DT, Zylstra GJ
  Title
Sequences of genes encoding naphthalene dioxygenase in Pseudomonas putida strains G7 and NCIB 9816-4.
  Journal
Gene 127:31-7 (1993)
DOI:10.1016/0378-1119(93)90613-8
Reference
  Authors
Dennis JJ, Zylstra GJ
  Title
Complete sequence and genetic organization of pDTG1, the 83 kilobase naphthalene degradation plasmid from Pseudomonas putida strain NCIB 9816-4.
  Journal
J Mol Biol 341:753-68 (2004)
DOI:10.1016/j.jmb.2004.06.034
  Sequence
Reference
  Authors
Lessner DJ, Johnson GR, Parales RE, Spain JC, Gibson DT
  Title
Molecular characterization and substrate specificity of nitrobenzene dioxygenase from Comamonas sp. strain JS765.
  Journal
Appl Environ Microbiol 68:634-41 (2002)
DOI:10.1128/AEM.68.2.634-641.2002
  Sequence
Reference
PMID:8759857
  Authors
Suen WC, Haigler BE, Spain JC
  Title
2,4-Dinitrotoluene dioxygenase from Burkholderia sp. strain DNT: similarity to naphthalene dioxygenase.
  Journal
J Bacteriol 178:4926-34 (1996)
DOI:10.1128/JB.178.16.4926-4934.1996
  Sequence
LinkDB

KEGG   ORTHOLOGY: K14580
Entry
K14580                      KO                                     

Name
nahAd, ndoC, nbzAd, dntAd
Definition
naphthalene 1,2-dioxygenase subunit beta [EC:1.14.12.12 1.14.12.23 1.14.12.24]
Pathway
ko00624  Polycyclic aromatic hydrocarbon degradation
ko00626  Naphthalene degradation
ko00627  Aminobenzoate degradation
ko00633  Nitrotoluene degradation
ko00642  Ethylbenzene degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01220  Degradation of aromatic compounds
Module
M00534  Naphthalene degradation, naphthalene => salicylate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00627 Aminobenzoate degradation
    K14580  nahAd, ndoC, nbzAd, dntAd; naphthalene 1,2-dioxygenase subunit beta
   00633 Nitrotoluene degradation
    K14580  nahAd, ndoC, nbzAd, dntAd; naphthalene 1,2-dioxygenase subunit beta
   00642 Ethylbenzene degradation
    K14580  nahAd, ndoC, nbzAd, dntAd; naphthalene 1,2-dioxygenase subunit beta
   00626 Naphthalene degradation
    K14580  nahAd, ndoC, nbzAd, dntAd; naphthalene 1,2-dioxygenase subunit beta
   00624 Polycyclic aromatic hydrocarbon degradation
    K14580  nahAd, ndoC, nbzAd, dntAd; naphthalene 1,2-dioxygenase subunit beta
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.12  With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
    1.14.12.12  naphthalene 1,2-dioxygenase
     K14580  nahAd, ndoC, nbzAd, dntAd; naphthalene 1,2-dioxygenase subunit beta
    1.14.12.23  nitroarene dioxygenase
     K14580  nahAd, ndoC, nbzAd, dntAd; naphthalene 1,2-dioxygenase subunit beta
    1.14.12.24  2,4-dinitrotoluene dioxygenase
     K14580  nahAd, ndoC, nbzAd, dntAd; naphthalene 1,2-dioxygenase subunit beta
Other DBs
RN: R02968 R05422 R05423 R05424 R05425 R05426 R05427 R06909 R06930 R06937 R07704 R07706 R09159 R09233
GO: 0018625
Genes
PPI: ND056(nahAd)
PSC: A458_06720
PSTU: UIB01_22630
PBM: CL52_14615
PFK: PFAS1_28615
ALT: ambt_17890
RMN: TK49_07955
BMK: DM80_1444(ndoC)
PARB: CJU94_38655
PNA: Pnap_2484
AJS: Ajs_3093
AG: AAB09767(dntAd) AAL76203(nbzAd) AAO64275(nahAd) ADQ90223(cnbAd) AGH09227(mntAd)
 » show all
Reference
PMID:8486285
  Authors
Simon MJ, Osslund TD, Saunders R, Ensley BD, Suggs S, Harcourt A, Suen WC, Cruden DL, Gibson DT, Zylstra GJ
  Title
Sequences of genes encoding naphthalene dioxygenase in Pseudomonas putida strains G7 and NCIB 9816-4.
  Journal
Gene 127:31-7 (1993)
DOI:10.1016/0378-1119(93)90613-8
Reference
  Authors
Dennis JJ, Zylstra GJ
  Title
Complete sequence and genetic organization of pDTG1, the 83 kilobase naphthalene degradation plasmid from Pseudomonas putida strain NCIB 9816-4.
  Journal
J Mol Biol 341:753-68 (2004)
DOI:10.1016/j.jmb.2004.06.034
  Sequence
Reference
  Authors
Lessner DJ, Johnson GR, Parales RE, Spain JC, Gibson DT
  Title
Molecular characterization and substrate specificity of nitrobenzene dioxygenase from Comamonas sp. strain JS765.
  Journal
Appl Environ Microbiol 68:634-41 (2002)
DOI:10.1128/AEM.68.2.634-641.2002
  Sequence
Reference
PMID:8759857
  Authors
Suen WC, Haigler BE, Spain JC
  Title
2,4-Dinitrotoluene dioxygenase from Burkholderia sp. strain DNT: similarity to naphthalene dioxygenase.
  Journal
J Bacteriol 178:4926-34 (1996)
DOI:10.1128/JB.178.16.4926-4934.1996
  Sequence
LinkDB

KEGG   ORTHOLOGY: K14578
Entry
K14578                      KO                                     

Name
nahAb, nagAb, ndoA, nbzAb, dntAb
Definition
naphthalene 1,2-dioxygenase ferredoxin component
Pathway
ko00624  Polycyclic aromatic hydrocarbon degradation
ko00626  Naphthalene degradation
ko00627  Aminobenzoate degradation
ko00633  Nitrotoluene degradation
ko00642  Ethylbenzene degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01220  Degradation of aromatic compounds
Module
M00534  Naphthalene degradation, naphthalene => salicylate
M00638  Salicylate degradation, salicylate => gentisate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00627 Aminobenzoate degradation
    K14578  nahAb, nagAb, ndoA, nbzAb, dntAb; naphthalene 1,2-dioxygenase ferredoxin component
   00633 Nitrotoluene degradation
    K14578  nahAb, nagAb, ndoA, nbzAb, dntAb; naphthalene 1,2-dioxygenase ferredoxin component
   00642 Ethylbenzene degradation
    K14578  nahAb, nagAb, ndoA, nbzAb, dntAb; naphthalene 1,2-dioxygenase ferredoxin component
   00626 Naphthalene degradation
    K14578  nahAb, nagAb, ndoA, nbzAb, dntAb; naphthalene 1,2-dioxygenase ferredoxin component
   00624 Polycyclic aromatic hydrocarbon degradation
    K14578  nahAb, nagAb, ndoA, nbzAb, dntAb; naphthalene 1,2-dioxygenase ferredoxin component
Other DBs
RN: R02968 R05422 R05423 R05424 R05425 R05426 R05427 R06909 R06930 R06937 R07704 R07706 R07709 R07710 R09159 R09233
COG: COG2146
Genes
EBT: EBL_c34290(ndoA)
PSD: DSC_14870
PPT: PPS_2607
PPI: ND054(nahAb)
PPUD: DW66_2865
PPJ: RK21_02167
PSC: A458_06730
PSTU: UIB01_22620
PBM: CL52_14605
PFK: PFAS1_28605
RMA: Rmag_0678
VOK: COSY_0630
PSE: NH8B_2003
RSO: RSc1088(nagAb)
RSL: RPSI07_2303(doxA)
RSM: CMR15_20207(doxA)
RSE: F504_1059
REH: H16_A1631(nagI)
BMK: DM80_1442(ndoA)
BUB: BW23_5817(ndoA)
BGU: KS03_3915(ndoA)
BXE: Bxe_C0940
BXB: DR64_7510(ndoA) DR64_8472
BFN: OI25_3048(ndoA)
PPNO: DA70_21380
PPNM: LV28_08940
PPUL: RO07_03170
PSPU: NA29_24080
PAPI: SG18_03360
BPAR: BN117_1348(ndoA)
BPA: BPP2189(ndoA)
BBH: BN112_1870(ndoA)
BBR: BB1586(ndoA)
BBM: BN115_1540(ndoA)
BBX: BBS798_1545(ndoA)
BPT: Bpet2806
AXX: ERS451415_00955(ndoA_1) ERS451415_02890(ndoA_2)
POL: Bpro_0985
AAV: Aave_0167
AJS: Ajs_3091
AAA: Acav_0198
CTES: O987_21885
LIM: L103DPR2_01956(ndoA)
LIH: L63ED372_02713(ndoA)
HYB: Q5W_18510
HPSE: HPF_02900(nagAb)
LCH: Lcho_0795
AZO: azo2514(nagAb)
AOA: dqs_2671
AZA: AZKH_1938
MSL: Msil_1467
JAN: Jann_3956
SHUM: STHU_47060
TMO: TMO_a0552(doxA)
NEV: NTE_01129
AG: AAA25905(nahAb) AAB09765(dntAb) AAL76201(nbzAb) AGH09225(mntAb)
 » show all
Reference
PMID:8486285
  Authors
Simon MJ, Osslund TD, Saunders R, Ensley BD, Suggs S, Harcourt A, Suen WC, Cruden DL, Gibson DT, Zylstra GJ
  Title
Sequences of genes encoding naphthalene dioxygenase in Pseudomonas putida strains G7 and NCIB 9816-4.
  Journal
Gene 127:31-7 (1993)
DOI:10.1016/0378-1119(93)90613-8
Reference
  Authors
Dennis JJ, Zylstra GJ
  Title
Complete sequence and genetic organization of pDTG1, the 83 kilobase naphthalene degradation plasmid from Pseudomonas putida strain NCIB 9816-4.
  Journal
J Mol Biol 341:753-68 (2004)
DOI:10.1016/j.jmb.2004.06.034
  Sequence
LinkDB

KEGG   ORTHOLOGY: K14581
Entry
K14581                      KO                                     

Name
nahAa, nagAa, ndoR, nbzAa, dntAa
Definition
naphthalene 1,2-dioxygenase ferredoxin reductase component [EC:1.18.1.7]
Pathway
ko00624  Polycyclic aromatic hydrocarbon degradation
ko00626  Naphthalene degradation
ko00627  Aminobenzoate degradation
ko00633  Nitrotoluene degradation
ko00642  Ethylbenzene degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01220  Degradation of aromatic compounds
Module
M00534  Naphthalene degradation, naphthalene => salicylate
M00638  Salicylate degradation, salicylate => gentisate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00627 Aminobenzoate degradation
    K14581  nahAa, nagAa, ndoR, nbzAa, dntAa; naphthalene 1,2-dioxygenase ferredoxin reductase component
   00633 Nitrotoluene degradation
    K14581  nahAa, nagAa, ndoR, nbzAa, dntAa; naphthalene 1,2-dioxygenase ferredoxin reductase component
   00642 Ethylbenzene degradation
    K14581  nahAa, nagAa, ndoR, nbzAa, dntAa; naphthalene 1,2-dioxygenase ferredoxin reductase component
   00626 Naphthalene degradation
    K14581  nahAa, nagAa, ndoR, nbzAa, dntAa; naphthalene 1,2-dioxygenase ferredoxin reductase component
   00624 Polycyclic aromatic hydrocarbon degradation
    K14581  nahAa, nagAa, ndoR, nbzAa, dntAa; naphthalene 1,2-dioxygenase ferredoxin reductase component
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.18  Acting on iron-sulfur proteins as donors
   1.18.1  With NAD+ or NADP+ as acceptor
    1.18.1.7  ferredoxin---NAD(P)+ reductase (naphthalene dioxygenase ferredoxin-specific)
     K14581  nahAa, nagAa, ndoR, nbzAa, dntAa; naphthalene 1,2-dioxygenase ferredoxin reductase component
Other DBs
RN: R02968 R05422 R05423 R05424 R05425 R05426 R05427 R06909 R06930 R06937 R07704 R07706 R07709 R07710 R09159 R09233
Genes
EBT: EBL_c34260(ndoR)
PPI: ND053(nahAa)
PSC: A458_06735
PSTU: UIB01_22615
PBM: CL52_14600
PFK: PFAS1_28600
ALT: ambt_18035
MMW: Mmwyl1_2668
MME: Marme_2403
MPC: Mar181_2356
RSO: RSc1091(nagAa)
RSL: RPSI07_2300(ndoR)
RSM: CMR15_20210(ndoR)
RSE: F504_1062
BMK: DM80_1434(ndoR) DM80_1439(ndoR)
BFN: OI25_3043(ndoR)
PPNO: DA70_21365
PPNM: LV28_08955
PPUL: RO07_03185
PSPU: NA29_24095
PAPI: SG18_03375
AFQ: AFA_02200
POL: Bpro_0980
PNA: Pnap_2489
AAV: Aave_0164
AJS: Ajs_3089
AAA: Acav_0195
LCH: Lcho_0800
TIN: Tint_1781
THI: THI_2205
AZO: azo2517(nagAa)
AOA: dqs_2674
SHZ: shn_02370
MNO: Mnod_0136
RBM: TEF_01260
PSF: PSE_0864
LABT: FIU93_04780(nagAa1)
SGI: SGRAN_2805(thnY)
TMO: TMO_2240(ndoR)
AG: AAA25904(nahAa) AAB09763(dntAa) AAD12606(nagAa) AAL76199(nbzAa) AGH09224(mntAa)
 » show all
Reference
PMID:8486285
  Authors
Simon MJ, Osslund TD, Saunders R, Ensley BD, Suggs S, Harcourt A, Suen WC, Cruden DL, Gibson DT, Zylstra GJ
  Title
Sequences of genes encoding naphthalene dioxygenase in Pseudomonas putida strains G7 and NCIB 9816-4.
  Journal
Gene 127:31-7 (1993)
DOI:10.1016/0378-1119(93)90613-8
Reference
  Authors
Dennis JJ, Zylstra GJ
  Title
Complete sequence and genetic organization of pDTG1, the 83 kilobase naphthalene degradation plasmid from Pseudomonas putida strain NCIB 9816-4.
  Journal
J Mol Biol 341:753-68 (2004)
DOI:10.1016/j.jmb.2004.06.034
  Sequence
LinkDB

KEGG   ENZYME: 1.14.12.12
Entry
EC 1.14.12.12               Enzyme                                 

Name
naphthalene 1,2-dioxygenase;
naphthalene dioxygenase;
naphthalene oxygenase;
NDO
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
Sysname
naphthalene,NADH:oxygen oxidoreductase (1,2-hydroxylating)
Reaction(IUBMB)
naphthalene + NADH + H+ + O2 = (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+ [RN:R02968]
Reaction(KEGG)
Substrate
naphthalene [CPD:C00829];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
(1R,2S)-1,2-dihydronaphthalene-1,2-diol [CPD:C04314];
NAD+ [CPD:C00003]
Comment
This enzyme is a member of the ring-hydroxylating dioxygenase (RHD) family of bacterial enzymes that play a critical role in the degradation of aromatic compounds, such as polycyclic aromatic hydrocarbons [5]. This enzyme comprises a multicomponent system, containing a reductase that is an iron-sulfur flavoprotein (FAD; EC 1.18.1.3, ferredoxin---NAD+ reductase), an iron-sulfur oxygenase, and ferredoxin. Requires Fe2+.
History
EC 1.14.12.12 created 1992
Pathway
ec00624  Polycyclic aromatic hydrocarbon degradation
ec00626  Naphthalene degradation
ec00642  Ethylbenzene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K14579  naphthalene 1,2-dioxygenase subunit alpha
K14580  naphthalene 1,2-dioxygenase subunit beta
Genes
PPI: ND055(nahAc) ND056(nahAd)
PSC: A458_06720 A458_06725
PSTU: UIB01_22625 UIB01_22630
PBM: CL52_14610 CL52_14615
PFK: PFAS1_28610 PFAS1_28615
ALT: ambt_17890 ambt_17895
RMN: TK49_07955
BMK: DM80_1443(ndoB) DM80_1444(ndoC)
PARB: CJU94_38655 CJU94_38660
PNA: Pnap_2484 Pnap_2485
 » show all
Reference
1  [PMID:6874638]
  Authors
Ensley BD, Gibson DT.
  Title
Naphthalene dioxygenase: purification and properties of a terminal oxygenase component.
  Journal
J Bacteriol 155:505-11 (1983)
DOI:10.1128/JB.155.2.505-511.1983
Reference
2  [PMID:234247]
  Authors
Jeffrey AM, Yeh HJ, Jerina DM, Patel TR, Davey JF, Gibson DT.
  Title
Initial reactions in the oxidation of naphthalene by Pseudomonas putida.
  Journal
Biochemistry 14:575-84 (1975)
DOI:10.1021/bi00674a018
Reference
3  [PMID:9634695]
  Authors
Kauppi B, Lee K, Carredano E, Parales RE, Gibson DT, Eklund H, Ramaswamy S
  Title
Structure of an aromatic-ring-hydroxylating dioxygenase-naphthalene 1,2-dioxygenase.
  Journal
Structure 6:571-86 (1998)
DOI:10.1016/S0969-2126(98)00059-8
Reference
4  [PMID:10692370]
  Authors
Parales RE, Lee K, Resnick SM, Jiang H, Lessner DJ, Gibson DT
  Title
Substrate specificity of naphthalene dioxygenase: effect of specific amino acids at the active site of the enzyme.
  Journal
J Bacteriol 182:1641-9 (2000)
DOI:10.1128/JB.182.6.1641-1649.2000
Reference
5  [PMID:17014090]
  Authors
Jouanneau Y, Meyer C, Jakoncic J, Stojanoff V, Gaillard J
  Title
Characterization of a naphthalene dioxygenase endowed with an exceptionally broad substrate specificity toward polycyclic aromatic hydrocarbons.
  Journal
Biochemistry 45:12380-91 (2006)
DOI:10.1021/bi0611311
Other DBs
ExplorEnz - The Enzyme Database: 1.14.12.12
IUBMB Enzyme Nomenclature: 1.14.12.12
ExPASy - ENZYME nomenclature database: 1.14.12.12
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.12.12
BRENDA, the Enzyme Database: 1.14.12.12
CAS: 9074-04-8
LinkDB

KEGG   REACTION: R06937
Entry
R06937                      Reaction                               

Name
naphthalene,NADH:oxygen oxidoreductase (1,2-hydroxylating)
Definition
2-Methylnaphthalene + Oxygen + NADH + H+ <=> cis-1,2-Dihydroxy-1,2-dihydro-7-methylnaphthalene + NAD+
Equation
Reaction class
RC00001  C00003_C00004
RC00091  C14098_C14102
Enzyme
Pathway
rn00626  Naphthalene degradation
rn01120  Microbial metabolism in diverse environments
Orthology
K14578  naphthalene 1,2-dioxygenase ferredoxin component
K14579  naphthalene 1,2-dioxygenase subunit alpha [EC:1.14.12.12 1.14.12.23 1.14.12.24]
K14580  naphthalene 1,2-dioxygenase subunit beta [EC:1.14.12.12 1.14.12.23 1.14.12.24]
K14581  naphthalene 1,2-dioxygenase ferredoxin reductase component [EC:1.18.1.7]
LinkDB

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