KEGG   ORTHOLOGY: K14585
Entry
K14585                      KO                                     

Name
nahE
Definition
trans-o-hydroxybenzylidenepyruvate hydratase-aldolase [EC:4.1.2.45]
Pathway
ko00626  Naphthalene degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01220  Degradation of aromatic compounds
Module
M00534  Naphthalene degradation, naphthalene => salicylate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00626 Naphthalene degradation
    K14585  nahE; trans-o-hydroxybenzylidenepyruvate hydratase-aldolase
Enzymes [BR:ko01000]
 4. Lyases
  4.1  Carbon-carbon lyases
   4.1.2  Aldehyde-lyases
    4.1.2.45  trans-o-hydroxybenzylidenepyruvate hydratase-aldolase
     K14585  nahE; trans-o-hydroxybenzylidenepyruvate hydratase-aldolase
Other DBs
RN: R05136
GO: 0018813
Genes
PPI: ND061(nahE)
PSC: A458_06700
PSTU: UIB01_22650
PBM: CL52_14635
PFK: PFAS1_28640
ALT: ambt_17910
CYQ: Q91_1157
CZA: CYCME_1270
CYY: CPC19_06820
GBI: PG2T_00740 PG2T_05760 PG2T_12860
BMK: DM80_1449(phdJ)
BFN: OI25_3035 OI25_3053(phdJ)
PNA: Pnap_2479
CID: P73_0351
NAR: Saro_3836
AG: AAA66357(nahE) AAD45417(nsaE)
 » show all
Reference
  Authors
Peng RH, Xiong AS, Xue Y, Fu XY, Gao F, Zhao W, Tian YS, Yao QH
  Title
Microbial biodegradation of polyaromatic hydrocarbons.
  Journal
FEMS Microbiol Rev 32:927-55 (2008)
DOI:10.1111/j.1574-6976.2008.00127.x
Reference
  Authors
Keck A, Conradt D, Mahler A, Stolz A, Mattes R, Klein J
  Title
Identification and functional analysis of the genes for naphthalenesulfonate catabolism by Sphingomonas xenophaga BN6.
  Journal
Microbiology 152:1929-40 (2006)
DOI:10.1099/mic.0.28783-0
  Sequence
LinkDB

KEGG   ENZYME: 4.1.2.45
Entry
EC 4.1.2.45                 Enzyme                                 

Name
trans-o-hydroxybenzylidenepyruvate hydratase-aldolase;
2'-hydroxybenzalpyruvate aldolase;
NsaE;
tHBPA hydratase-aldolase
Class
Lyases;
Carbon-carbon lyases;
Aldehyde-lyases
Sysname
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate hydro-lyase
Reaction(IUBMB)
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O = salicylaldehyde + pyruvate [RN:R05136]
Reaction(KEGG)
R05136
Substrate
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate [CPD:C06203];
H2O [CPD:C00001]
Product
salicylaldehyde [CPD:C06202];
pyruvate [CPD:C00022]
Comment
This enzyme is involved in naphthalene degradation. The enzyme catalyses a retro-aldol reaction in vitro, and it accepts a broad range of aldehydes and 4-substituted 2-oxobut-3-enoates as substrates [4].
History
EC 4.1.2.45 created 2010, modified 2011
Pathway
ec00626  Naphthalene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K14585  trans-o-hydroxybenzylidenepyruvate hydratase-aldolase
Genes
PPI: ND061(nahE)
PSC: A458_06700
PSTU: UIB01_22650
PBM: CL52_14635
PFK: PFAS1_28640
ALT: ambt_17910
CYQ: Q91_1157
CZA: CYCME_1270
CYY: CPC19_06820
GBI: PG2T_00740 PG2T_05760 PG2T_12860
BMK: DM80_1449(phdJ)
BFN: OI25_3035 OI25_3053(phdJ)
PNA: Pnap_2479
CID: P73_0351
NAR: Saro_3836
 » show all
Reference
1  [PMID:8486638]
  Authors
Kuhm AE, Knackmuss HJ, Stolz A
  Title
Purification and properties of 2'-hydroxybenzalpyruvate aldolase from a bacterium that degrades naphthalenesulfonates.
  Journal
J Biol Chem 268:9484-9 (1993)
Reference
2  [PMID:16804169]
  Authors
Keck A, Conradt D, Mahler A, Stolz A, Mattes R, Klein J
  Title
Identification and functional analysis of the genes for naphthalenesulfonate catabolism by Sphingomonas xenophaga BN6.
  Journal
Microbiology 152:1929-40 (2006)
DOI:10.1099/mic.0.28783-0
  Sequence
Reference
3  [PMID:8002605]
  Authors
Eaton RW
  Title
Organization and evolution of naphthalene catabolic pathways: sequence of the DNA encoding 2-hydroxychromene-2-carboxylate isomerase and trans-o-hydroxybenzylidenepyruvate hydratase-aldolase from the NAH7 plasmid.
  Journal
J Bacteriol 176:7757-62 (1994)
DOI:10.1128/JB.176.24.7757-7762.1994
  Sequence
Reference
4  [PMID:10831455]
  Authors
Eaton RW
  Title
trans-o-Hydroxybenzylidenepyruvate hydratase-aldolase as a biocatalyst.
  Journal
Appl Environ Microbiol 66:2668-72 (2000)
DOI:10.1128/AEM.66.6.2668-2672.2000
Other DBs
ExplorEnz - The Enzyme Database: 4.1.2.45
IUBMB Enzyme Nomenclature: 4.1.2.45
ExPASy - ENZYME nomenclature database: 4.1.2.45
BRENDA, the Enzyme Database: 4.1.2.45
LinkDB

KEGG   REACTION: R05136
Entry
R05136                      Reaction                               

Name
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate hydro-lyase
Definition
Salicylaldehyde + Pyruvate <=> trans-o-Hydroxybenzylidenepyruvate + H2O
Equation
Reaction class
RC00924  C06202_C06203
RC00936  C00022_C06203
Enzyme
Pathway
rn00626  Naphthalene degradation
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01220  Degradation of aromatic compounds
Module
M00534  Naphthalene degradation, naphthalene => salicylate
Orthology
K14585  trans-o-hydroxybenzylidenepyruvate hydratase-aldolase [EC:4.1.2.45]
Other DBs
RHEA: 27392
LinkDB

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